Z-Hun Kim

Hu evergreenpharm

Lumostatic cultures of Haematococcus pluvialis were performed and compared using three different types of specific light uptake rate, qe: cell concentration, cell projection area, and fresh weight. These three parameter are representing one, two, and three-dimensional characteristic values of the biomass, respectively.The qe value using cell concen...

The effect of light is undoubtedly the most important factor of the astaxanthin accumulation in Haematococcus pluvialis. A suitable light source with adequate light intensity is required to accumulate a high-level of astaxanthin. Flashing light has been drawing an increasing interest as a potential alternative light source to enhance the efficiency...

Botryococcus braunii has an outstanding ability to produce lipid; however, it is a slow-growing green microalgae. Statistical optimization of growth media was performed to faster growth and to increase lipid concentration. The effect of media composition on the growth of B. braunii LB572 was examined using fractional factorial design and central co...

The objective of this study is developing microalgal culture systems to produce biodiesel with low-energy inputs in the ocean. The semi-permeable membrane photobioreactors (SPM-PBRs), which are capable of transferring nutrients dissolved in seawater into the algal broth while containing the cells inside, were operated in a reservoir containing Inch...

Salt stress can be used to increase intracellular lipid content of microalgae. However, the growth of microalgae is decreased under salt stress. Thus, a two-stage cultivation strategy with a growth phase followed by a stress stage is required to improve lipid content. In this study, adaptive laboratory evolution (ALE) with salt stress was conducted...

Mychonastes sp. 247 is a newly isolated freshwater microalga. Mychonastes sp. 247 methanolic extract (ME) suppresses the growth of A549 human non-small-cell lung cancer (NSCLC) cells. However, the underlying mechanism of this anticancer effect remains unclear. The present study aimed to reveal the underlying mechanism of the anticancer effect of ME...

This study aimed to evaluate the effects of dietary Chlorella vulgaris (CV) on the distribution of immune cells, intestinal morphology, intestinal barrier function, antioxidant markers, and the cecal microbiome in 10-day-old broiler chickens. A total of 120 day-old Ross 308 male broiler chicks were assigned to two dietary treatments using a randomi...

Microalgae have been known as superior producers of valuable carotenoids via their defensive mechanism against environmental changes. In this study, nitrogen and phosphorus starvation (NPS), high light intensity (HL), and high salinity (HS) stress culture conditions were subjected to indigenous freshwater microalga, Mychonastes pushpae culture to i...

Simple Summary In the agricultural sector, particularly the poultry industry, antibiotics have been used as feed additives to treat and prevent infections or to improve the growth, health, and welfare of chickens. Many nutritional alternative strategies, including natural ingredients, have been investigated for their role in improving gut health wi...

Previously, we confirmed that Mychonastes sp. 246 methanolic extract (ME) markedly reduced the viability of BxPC-3 human pancreatic cancer cells. However, the underlying mechanism ME remained unclear. Hence, we attempted to elucidate the anticancer effect of ME on BxPC-3 human pancreatic cancer cells. First, we investigated the components of ME and...

In this study, culture conditions were optimized using response surface analysis to enhance the cell growth and lutein production of Chlorella miniata, a freshwater microalgae received at the freshwater bioresources culture collection of the Nakdonggang National Institute of Biological Resources. The main independent variables selected for the cult...

In this study we sought to improve lutein and zeaxanthin production in Mychonastes sp. 247 and investigated the effect of environmental factors on lutein and zeaxanthin productivity in Mychonastes sp. The basic medium selection and N:P ratio were adjusted to maximize cell growth in one-stage culture, and lutein and zeaxanthin production conditions...

In this study, we investigated the effect of enhanced collagen synthesis by Mychonastes sp. 249 (a type of freshwater microalgae) extract (MSE) on skin regeneration. The effect of MSE on human dermal fibroblast (HDF) migration was studied to confirm its skin regenerative effect, and at 1.0 mg/mL MSE increased HDF cell migration by 21.2%. To stud...

Gossypol, a natural phenolic aldehyde present in cotton plants, was originally used as a means of contraception, but is currently being studied for its anti-proliferative and anti-metastatic effects on various cancers. However, the intracellular mechanism of action regarding the effects of gossypol on pancreatic cancer cells remains unclear. Here,...

Lactic acid bacteria (LAB) are probiotic candidates that may restore the balance of microbiota populations in intestinal microbial ecosystems by controlling pathogens and thereby promoting host health. The goal of this study was to isolate potential probiotic LAB strains and characterize their antimicrobial abilities against pathogens in intestinal...

Microalgae-derived docosahexaenoic acid (DHA) production has been considered an alternative technology to overcome several disadvantages of fish oil-based DHA production. Despite its potential, efficient production methods for DHA have been rarely reported. Accordingly, in this study we investigated the potential application of Jerusalem artichoke...

In the present work, we report the complete genome sequence of Lactobacillus harbinensis NSMJ42, isolated from makgeolli (a Korean traditional alcoholic beverage) in South Korea. The final genome assembly consists of a 3.29-Mbp chromosome with 3,082 protein-coding sequences and a G+C content of 53.36%.

Microalgal biomass productivity of semi-permeable membrane photobioreactors (SPM-PBRs) highly depends on diffusion of nutrient ions from surrounding environment through SPMs. The objective of this study was to enhance microalgal biomass productivity in floating photobioreactors (PBRs) with semi-permeable membranes (SPMs) grafted with 4-hydroxyphene...

For successful microalgal biodiesel production, the strain should be selected carefully. Fast growth rate and high fatty acid contents are desired traits for algal biodiesel production. In ocean cultivation of microalgae, seawater temperature slowly changes over seasons, and rotating algal strains in accordance with their optimal temperature could...

In this study, hypersaline media were used for ocean cultivation of the marine microalga Tetraselmis sp. KCTC12432BP for enhanced biomass and fatty acid (FA) productivity. Hypersaline media (55, 80, and 105 PSU) were prepared without sterilization by addition of NaCl to seawater obtained from Incheon, Korea. The highest biomass productivity was obt...

Novel carbon-based solid acid catalysts have been synthesized through a sustainable route fromlipid extracted microalgal residue ofDunaliella tertiolecta, for biodiesel production. Two carbon based solid acid catalysts were prepared by surface modification of bio-char with sulfuric acid (H2SO4) and sulfuryl chloride (SO2Cl2), respectively. Treated...

Lipids in microalgae are energy-rich compounds and considered as an attractive feedstock for biodiesel production. To redirect carbon flux from competing pathways to the fatty acid synthesis pathway of Tetraselmis sp., we used three types of chemical inhibitors, which can block the starch synthesis pathway or photorespiration, under nitrogen-suffic...

Various polymers were used as transfection factors for small interfering RNA (siRNA) to effectively suppress human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) gene in transgenic rice cells. Five kinds of polymers (PEI, PVA, PVP, and 8 and 20 kDa PEGs) were applied for delivery of siRNA with lipofectamine used as a control. In the cyt...

Some artificial light sources able to emit photons at specific wavelengths, such as LEDs, are useful for studying the effects of light quality on microalgal growth and production of fatty acids; however, they should not be used for outdoor cultivation of microalgae to produce bioenergy. Instead, various light filters capable of selectively transmit...

The purpose of this study was to inhibit biofouling on a selectively permeable membrane (SPM) and increase biomas productivity in marine photobioreactors (PBRs) for microalgal culti- vation by chemical treatment. Surfaces of a SPM, composed of polyethylene terephthalate (PET), was sulfonated to decrease hydrophobicity through ataching negatively...

Cost-effective microalgae harvesting methods are necessary for economical production of algal biodiesel. In this study, membranes with various pore sizes and materials were examined for their potentials in application to gravity-filtration of Tetraselmis sp. KCTC12432BP. For this test, 10 L of Tetraselmis sp. culture (2 g/L) was loaded on each memb...

Microalgae are attracting much attention as superior biodiesel producers. In particular, under stressful conditions, they accumulate organic compounds consisting entirely of carbon and hydrogen. The aim of this work was to increase intracellular fatty acid content in Dunaliella tertiolecta (Chlorophyceae), Nannochloropsis oculata (Eustigmatophyceae...

Chemically-processed clays (PRCs) by base and acid treatments were developed to effectively remove microalgae, and their removal efficiencies were evaluated and compared to that of natural clay. The processed clays were produced by using sodium hydroxide and different amounts of sulfuric acid, and their characteristics were analyzed. Microalgae rem...

In the present study, we evaluated the in vitro wound-healing properties of two types of rice cell extracts (RCEs; prepared using ethanol and pressurized hot water extraction methods), using human dermal fibroblasts and keratinocytes. The effects of the RCEs (at 25–100 µg/ml) on cytotoxicity and cell migration were assessed. Both RCEs were not cyto...

We investigated the regenerative effects and regulatory mechanisms of human umbilical cord mesenchymal stem cells (UC-MSCs)-derived conditioned medium (CM) in atrophied muscles using an in vivo model. To determine the appropriate harvest point of UC-CM, active factor content was analyzed in the secretome over time. A muscle atrophy model was induce...

Microalgae are considered as superior biodiesel producers, because they could effectively produce high amount of lipid with fast growth rate. In this study, Chlorella vulgaris was exposed to various light wavelengths (λmax 470 nm, λmax 525 nm, and λmax 660 nm) using light emitting diodes (LEDs) to examine effect of light quality on their growth and...

It is important to design photobioreactor by cheap material for economical microalgal biomass production. In this study, two types of marine photobioreactors (MPBR), made by either polyvinyl chloride (MPBR-PVC) or high density poly ethylene (MPBR-HDPE), are used and performance of these were compared. Tetraselmis sp. KCTC 12236BP is a green marine...

The microalgae have been studied for a source of biodiesel production. It is important to select the microalgae, which grows rapidly in local environmental conditions such as temperature range and ingredient of local seawater. The aim of this study was isolating microalga, which has rapid growth rate and high FAME contents in wide temperature range...

Culturing microalgae in the ocean has potentials that may reduce the production cost and provide an option for an economic biofuel production from microalgae. The ocean holds great potentials for mass microalgal cultivation with its high specific heat, mixing energy from waves, and large cultivable area. Suitable photobioreactors (PBRs) that are ca...

Lumostatic operation was applied for efficient astaxanthin production in autotrophic Haematococcus lacustris cultures using 0.4-L bubble column photobioreactors. The lumostatic operation in this study was performed with three different specific light uptake rates (q e) based on cell concentration, cell projection area, and fresh weight as one-, two...

A split-column photobioreactor (SC-PBR), consisting of two bubble columns with different sizes, was developed to enhance photon utilization efficiency in an astaxanthin production process from Haematococcus lacustris. Among the two columns, only the smaller column of SC-PBR was illuminated. Astaxanthin productivities and photon efficiencies of SC-P...

Chinese hamster ovary (CHO) cells have been widely used for production of various recombinant proteins such as cytokines and monoclonal antibodies. The cell aggre-gation and cell death in CHO cell culture directly affect cell viability, and productivity and quality of products. In this study, we investigated preventing effects of storage-protein 2...

A green microalga, Tetraselmis sp., was cultivated in the coastal seawater of Young-Heung Island using semi-permeable membrane photobioreactors (SPM-PBRs) in different seasons. The microalgae in SPM-PBRs were able to grow on nutrients diffused into the PBRs from the surrounding seawater through SPMs. The biomass productivity varied depending on the...

Cryopreservation methods for human mesenchymal stem cells (hMSCs) typically depend on the presence of fetal bovine serum (FBS) with dimethyl sulphoxide (DMSO), which is not appropriate for therapeutic applications. In our previous study, we found that storage protein 2 (SP2), a natural material derived from silkworm hemolymph, has an inhibitory eff...

The purpose of this study was to investigate a relationship between ultraviolet radiation and initial cell density (ICD) of microalgae using a floating marine photobioreactor (PBR). To examine the effect of ultraviolet (UV) radiation in sunlight on biomass productivity as a function of ICD, 0.5-L floating PBRs covered with or without UV cut-off fil...

The present invention provides a photobioreactor for the economical and effective mass production of a photosynthetic microorganism, comprising a culture container capable of shape maintenance, which can hold a photosynthetic microorganism to be cultured and a liquid for dispersing the photosynthetic microorganism, wherein the whole or at least a p...

The therapeutic potential of mesenchymal stem cell-conditioned medium (MSC-CM) has been reported with various types of disease models. Here, we examine the therapeutic effect of umbilical cord MSC-CM (UCMSC-CM) on muscle-related disease, using a dexamethasone (Dex)-induced muscle atrophy in vitro model. The expressions of muscle atrophy-related pro...

In this article, we demonstrate that hydrogel-based composite membranes are used as semipermeable materials for the construction of photobioreactors (PBRs). PBRs are developed to culture microalgae using nutrients dissolved in seawater, and thus they need to be fabricated with membranes possessing sufficient material-transport properties. While hyd...

In this study, we developed a novel composite filler comprising cross-linked hyaluronic acid (HA) and human collagen (COL) derived from the human umbilical cord with the aim of improving biocompatibility and longevity compared to commercially available fillers. After HA/COL composite fillers were made in two different ratios (10:1 and 5:1), physica...

Abstract Objective: The protective effects of human mesenchymal stem cells (MSCs) isolated from different sources (bone marrow, adipose tissue and umbilical cord) on skeletal muscle regeneration were investigated using a hindlimb suspension (HS)-induced muscle atrophy rodent model. Methods: Female SD rats were randomly assigned to three groups:...

Objective: The protective effects of human mesenchymal stem cells (MSCs) isolated from different sources (bone marrow, adipose tissue and umbilical cord) on skeletal muscle regeneration were investigated using a hindlimb suspension (HS)-induced muscle atrophy rodent model. Methods: Female SD rats were randomly assigned to three groups: control, hi...

The present invention provides a novel Tetraselmis sp. MBEyh05Gc strain (deposit number KCTC 12433BP) which grows at 0-37[deg.]C, and a method for producing biodiesel using the same.

This study investigated the effects of silkworm hemolymph-derived storage protein 2 (SP2) on the whitening process in mouse B16F1 melanoma cells. After the cells were treated with various con-centrations of SP2 (0.1 -1.0 mg/mL), cytotoxicity, melanin contents, and differences in mRNA and protein expression associated with melanogenesis were observe...

Biodiesel production from microalgae is a novel green technology designed to cope with fossil fuel depletion and climate change. Photobioreactors (PBRs) have been used to maintain optimum culture conditions for microalgae, including light, temperature, CO2, and nutrients, to produce a stable year-round biomass yield. To design a good PBR, the techn...

This review describes about the relationship between bio-diesel production and composition of polysaccharides derived from micoralga such as Tetraselmis sp., Dunaliella tertiolecta, Botryococcus brauni, and Nannochloropsis oculata. The monosac�charide composition of the polysaccharides obtained from those de-fatted cell walls was elucidated by HPAE...

Published online The popularity of photo-bioreactors (PBRs) for the cultivation of microalgae has increased in the last decade because they can provide a suitable environment in terms of light, nutrients, CO 2, and temperature. Among the many types of PBRs, the bubble-column type is very attractive because of its simple design, easy operation, and...

A water-soluble polysaccharide was isolated and purified from the culture filtrate of the photosynthetic green microalgae Haematococcus lacustris by 75% ethanol precipitation and Sepharose CL-6B column chromatography. The molecular mass of the purified polysaccharide (named HCP) was estimated to be approximately 135 kDa by size-exclusion HPLC and i...

Astaxanthin production is commonly induced under stress conditions such as nutrient deficiency (N or P), high light stress, and variations of temperature, high NaCl concentrations, and other factors. The objective of the present study is the analysis of the effect of oxidative stress by sodium orthovanadate (SOV), a nonspecific inhibitor of protein...

The unicellular green alga, Haematococcus pluvialis, accumulates the highest level of astaxanthin among knownastaxanthi.n-producing organisms. Light is the most important factor to induce astaxanthin by H. pluvialis. BIue andred LEDs, whose 's are 470 and 665 nm, respectively, were used for internally illuminated light sources.Fluorescent lamps wer...

The aim of the present study is to define the influence of N-acetylglucosamine (GlcNAc) in heterotrophic culture of photosynthetic microalgae Chlorellar vulgaris, which can utilize insoluble colloidal chitin supplemented as a sole carbon source in heterotrophic condition, as described in our previous work. For the deep understanding of the cataboli...

Lumostatic operation for cultivation of Haematococcus pluvialis was assessed to test the scale-up strategy of photobioreactors. Lumostatic operation is a method of maintaining a proper light condition based on the specific light uptake rate (qe), by cells. Lumostatic operations were performed in 0.4-, 2-, 10-, and 30-l scale bubble column photobior...