Yusuf Ozsensoy

Cumhuriyet University, Faculty of Veterinary Medicine · Deparment of Veterinary Genetics

Sense of hearing is of crucial importance for dogs to perform their duties.There is no study to determine the reference latency intervals of dogs' sense of hearing.The aim of this study is to identify normative data that can be used as the reference interval for the wave latencies I,III and V at 80 dB with respect to the sense of hearing of Kangal...

Effects of situations in which Kangal dogs used as herding dogs and guarding dogs on blood parameters are unknown. Objective of this study was comparison of hematological blood levels of Kangal dogs which are used as herding dogs and guarding dogs. A total of 48 blood samples were collected from Kangal dogs which are used as herding dogs and guardi...

Water buffalo breeding is practiced in various provinces of Turkey, and is also included in the National Breeding Project. It was aimed in this study to investigate polymorphisms on exon 4–intron 5 and exon 4 only of the kappa-casein (CSN3) gene for milk yields of water buffalo bred in Sivas Province. Blood samples were taken from 135 water buffalo...

The aim of this study was to determine the genetic variation of Colored horses by using microsatellite markers. In the study, samples from a total of 28 Colored horse were genotyped using 17 microsatellite markers, 12 of them are recommended by the International Society for Animal Genetics (ISAG) and the other 5 loci included by the StockMarks Geno...

Sense of hearing is of crucial importance for dogs to perform their duties. There is no study to determine the reference latency intervals of dogs' sense of hearing. The aim of this study is to identify normative data that can be used as the reference interval for the wave latencies I, III and V at 80 dB with respect to the sense of hearing of Kang...

Water buffalo breeding by breeder project is ongoing in Sivas province as well as in many other provinces of Turkey Republic. The aim of this study was to investigate gene polymorphisms on Exon 4 – Intron 5 and only Exon 4 of kappa casein (K-CN) gene for milk productivity in Sivas province. Blood samples were taken from a total of 135 water buffalo...

The aim of this study was to determine the genetic structure of Coloured horses in Turkey by analysis of the D-loop sequence of mitochondrial DNA (mtDNA). A total of 28 Coloured horses were examined. DNA was extracted from blood samples using DNA isolation kit; 519 bp long mtDNA D-loop region was amplified by PCR and sequenced by capillary electrop...

Kangal shepherd dogs have excellent sensory organs and perform missions perfectly. For this reason, in many countries of the world, they are preferred for herd guard and as shepherd dog. One of these sensory organs is hearing. The aim of this study was to evaluate the deafness risk estimation analysis of Kangal shepherd dogs breeding in Sivas provi...

The aim of this study was to compare Kangal shepherd dogs with and without ankyloglossia in terms of various physiological and behavioral changes. A total of 24 dogs were used, 11 of which have had ankyloglossia and 13 of which did not have ankyloglossia. Heart rates, respiratory rates, and body temperatures of the dogs were measured at three diffe...

A total of 20 microsatellite DNA markers were used for genetic characterization and determination of phylogenetic relationships of native cattle breeds of Turkey, including the Anatolian Grey (AG), Anatolian Black (AB), South Anatolian Red (SAR), East Anatolian Red (EAR), Southern Anatolian Yellow (SAY), and Zavot (ZAV). DNA samples were isolated f...

In this study, we aimed to determine the gene polymorphisms of antioxidant enzymes that determine or affect antioxidant activity in the occurrence of the disease and/or complications during and after the surgery in patients who were decided to undergo coronary artery bypass surgery due to coronary artery disease. Blood samples taken before operatio...

Introduction: The aim of this study was to determine the genetic polymorphisms of some antioxidant enzymes together with oxidative stress and the response of some antioxidant enzymes against this situation in vascular and endovascular interventions performed for diseases of the infrarenal abdominal aorta. Material and methods: Twenty-four curren...

Background: The aim of the present study is to determine the correlation between the oxidative stress marker and the antioxidant parameters with the aortic cross clamp and pump times and to make contribution to the related studies. Methods: Fifty four elective patients for whom cardiopulmonary bypass operation was planned with different indication...

The aim is to compare some physiological parameters of Kangal dogs in three different (18-23-28ºC) environmental temperatures. Study was included 24 healthy Kangal dogs from Sivas of which 11 were with and 13 were without the ankyloglossia. Pulse, ventilation, and bodily temperatures were measured both during resting and at ends of 5-10-15 minutes...

The present study was aimed to conduct deafness risk estimation analyses of Kangal shepherd dogs that were raised in Sivas province of Turkey. Brainstem Auditory Evoked Response (BAER) test was conducted for to screen deafness in 160 Kangal shepherd dogs that were raised in centrum and towns of Sivas province. Test subjects were not isolated from t...

The objective of this study was to investigate polymorphisms both in exons 1 and 3 of prolactin (PRL) gene for milk productivity of Anatolian water buffalo breed in Sivas province in Turkey. Blood samples were collected from 129 male and female water buffaloes and DNA was isolated by using phenol/chloroform method. Samples of DNA were amplified and...

Kangal köpeklerinde düzenli sürü koruma görevini yerine getirme ve bekçilik amacıyla bağlı tutulma durumlarının kan parametrelerine olan etkileri bilinmemektedir. Bu çalışmanın amacı sürü koruma köpeği olan ve kulübe şartlarında bekçilik görevi yapan Kangal köpeklerinin hematolojik düzeylerinin karşılaştırılmasıdır. Bu çalışmada, sürü koruma köpeği...

In the current study, the mtDNA D-Loop region was analyzed in South Anatolian Red (SAR, n=51), Anatolian Black (NB, n=50), Anatolian Grey (AG, n=54), Native Southern Anatolian Yellow (NSAY, n=51), East Anatolian Red (EAR, n=54) and Zavot (ZAV, n=19) cattle breeds (n=279) to reveal diversity of mitochondrial DNA, differentiation of breeds, and relev...

Sunulan çalışmada, Türkiye yerli sığır ırklarında DGAT1 geni K232A ile PRNP geni promotor ve intron 1 bölgelerinde indel polimorfizmleri araştırılmıştır. Çalışmada materyal olarak, Boz Irk (BI), Yerli Kara (YK), Doğu Anadolu Kırmızısı (DAK), Güney Doğu Anadolu Kırmızısı (GAK) ve Zavot ırklarından olmak üzere toplam 122 kan örneği kullanıldı. Örnekl...

Objective: The identification of meat species in meat products is important for protection of human health, economic reasons, religious factors and for controlling the compliance with food regulations. For this purpose, DNA must be obtained in good quality and quantity. The aim of this study was to compare different DNA isolation methods from diffe...

Aims of this study, were comparisons of some blood parameters which includes leucocyte count (WBC), erythrocyte count (RBC), Hemoglobin (Hg), Hematocrit level (HCT), Platelets count (PLT) and mean volume of erythrocytes (MCV) and some macro element levels which includes iron (Fe), calcium (Ca), phosphor (P) and magnesium (Mg) in both naturally infe...

Et ürünlerinde kullanılan et türlerinin tespiti insan sağlığının korunması, ekonomik sebepler, dini faktörler ve gıda mevzuatına uygunluğun kontrolü açısından önemlidir. Bu amaçla da ilk önce iyi kalitede ve miktarda DNA elde edilmesi gerekmektedir. Bu çalışmanın amacını farklı et ürünlerinde farklı DNA izolasyon yöntemlerinin karşılaştırılması olu...

Amaç: Bu çalışmada Sivas ilinde piyasaya sunulan et ürünlerinde farklı yöntemler kullanılarak tür tayinin yapılması ve bu yöntemlerin karşılaştırılması amaçlanmıştır. Gereç ve Yöntem: Bu çalışmanın materyalini, Sivas’ta 2014 yılında 6 farklı satış noktasından rastgele örnekleme yöntemiyle alınan sucuk, salam, sosis ve köfte örnekleri oluşturdu. Et...

The objective of this study was to determine the intra- and intergenetic diversities of eight different goat populations in Turkey including Hair, Angora, Kilis, Yayladag, Shami, Honamli, Saanen, and Alpine. A total of 244 DNA samples were genotyped using 11 microsatellites loci. The genetic differentiation between breeds was considerable as a resu...

Genetic characterization of populations and thereby determination of diversity are critically important for development of management programs for genetic resources and determination of population migrations in the history. The aim of this study was to determine genetic characterization of cat breeds reared in Turkey using microsatellite DNA marker...

Amaç: Bu çalışmada Sivas ilinde piyasaya sunulan et ürünlerinde farklı yöntemler kullanılarak tür tayinin yapılması ve bu yöntemlerin karşılaştırılması amaçlanmıştır. Ayrıca et ürünleri histolojik yönden de araştırılmıştır. Materyal ve Metot: Bu çalışmanın materyalini, Sivas’ta 2014 yılında 7 farklı satış noktasından rastgele örnekleme yöntemiyle a...

Genetic diversity amongst plant species offers prospects for improving the plant characteristics. Its assessment is necessary to help tackle the threats of environmental fluctuations and for the effective exploitation of genetic resources in breeding programmes. Although wheat is one of the most thoroughly studied crops in terms of genetic polymorp...

Characterization of populations at the molecular level provides an estimation of genetic diversity and distances between and within the populations. For this purpose, microsatellites are widely preferred marker systems in population analyses. Genotypic data at DNA level were also used to test genetic relationships between individuals in a populatio...

Characterization of populations at the molecular level provides an estimation of genetic diversity and distances between and within the populations. For this purpose, microsatellites are widely preferred marker systems in population analyses. Genotypic data at DNA level were also used to test genetic relationships between individuals in a populatio...

Objective of this study was to evaluate potential extinction risk of Turkish native cattle breeds using Mantel and AMOVA tests and Bottleneck analysis. A total of 271 DNA samples were isolated from Anatolian Black, Anatolian Grey, South Anatolian Red, Native Southern Anatolian Yellow, East Anatolian Red and Zavot cattle. Genotypes of twenty microsa...

Objective of this study was to evaluate microsatellite markers in paternity testing of native cattle breeds in Turkey. Blood samples were collected from Anatolian Black (n=51), Anatolian Grey (n=54), South Anatolian Red (n=51), Native Southern Anatolian Yellow (n=51), East Anatolian Red (n=45) and Zavot (n = 19) cattle. From the blood samples DNA w...

Giriş: Bu çalışmanın amacı, Y kromozomuna spesifik 7 mikrosatellit DNA markörleri kullanılarak 6 Türkiye yerli sığır ırkının filogenetik ilişkisinin tespit edilmesidir. Materyal ve Metot: Yerli Kara (YK), Boz Irk (BOZ), Güneydoğu Anadolu Kırmızısı (GAK), Yerli Güney Sarısı (YGS), Doğu Anadolu Kırmızısı (DAK) ve Zavot (ZAV) ırklarından standart feno...

Amaç: Çiğ et, kıyma, hazırlanmış et karışımları ve et ürünlerinin piyasaya arzına ilişkin hususlar 05.12.2012 tarih ve 28488 sayılı Resmi Gazete’de yayımlanan, 2012/74 sayılı "Türk Gıda Kodeksi Et ve Et Ürünleri Tebliği” ne göre büyükbaş ve küçükbaş etleri kendi aralarında ve kanatlı etleri kendi aralarında karıştırılabilir, fakat farklı hayvan tür...

The aim of this study was to determine phylogenetic relationships of 6 native cattle breeds of Turkey using 7 Y chromosome specific microsatellite DNA markers. DNA samples were isolated from Anatolian Black, Anatolian Grey, South Anatolian Red, Native Southern Anatolian Yellow, East Anatolian Red, and Zavot cattle using a standard phenol/chloroform...

Objective of this study was to evaluate microsatellite markers in paternity testing of native cattle breeds in Turkey. Blood samples were collected from Anatolian Black (n=51), Anatolian Grey (n=54), South Anatolian Red (n=51), Native Southern Anatolian Yellow (n=51), East Anatolian Red (n=45) and Zavot (n = 19) cattle. From the blood samples DNA w...

Sirke sinekleri (Drosophilia melanogaster) üzerinde yapılan çalışmalarda, bazı özelliklerinin kalıtımının Mendel Kanunlarına uymadığı ve genlerin kromozomlar üzerinde bileşik olabileceği belirlenmiştir. Dolayısıyla, genetik linkage (bağlantı) kavramı ve ilk genetik bağlantı haritası başarıyla ilk kez ortaya konulmuştur. Gen haritalaması, genlerin v...

Aim: The objective of the present study was identification of the chromosomal regions responsible for egg yield and body weight at different age periods in a Denizli and White Leghorn F2 population. Materials and Methods: An experimental F2 population was constructed by crossing Denizli and White Leghorn breeds and the yields of the animals were re...

Moleküler biyoloji alanındaki gelişmeler sonucunda günümüzde markörler genel olarak genomun özgün bir bölgesini tanımlamak amacıyla kullanılmaktadır. Genom analizleri ve genetik çalışmalarda morfolojik, protein ve DNA markörleri olmak üzere üç tip markör kullanılmaktadır. Polimeraz zincir reaksiyonunun (PZR) keşfinden sonra genetik çalışmalarda PZR...

Deli Dana Hastalığı (BSE) sonrası özellikle ruminant kökenli rendering ürünlerinin hayvan rasyonlarında kullanılması kontrol altına alınmıştır. Gümrük Beyan Belgesinde “hidrolize tüy unu” olarak bildirilen yem materyalinden alınan örnek, T.C. Gümrük Müsteşarlığı Mersin Gümrük Müdürlüğü tarafından gönderilerek hayvansal kökeninin belirlenmesi istenm...

Nowadays, owing to the developments in molecular biology, genetic markers are generally used to describe specific regions of the genome. Three different marker systems, namely, protein and DNA markers, are used in genome analyses and in various genetic studies. Following the discovery of polymerase chain reaction (PCR), PCR-based marker systems are...

As part of a national project titled “In vitro Conservation and Preliminary Molecular Identification of Some Turkish Domestic Animal Genetic Resources‐I (TURKHAYGEN‐I)”, genetic structures, evolutionary relationships and genetic diversities were investigated among six local cattle breeds in Turkey. For DNA isolation, 271 blood samples were collecte...

Giriş ve Amaç: Domuz gribi, Mart 2009’da bir domuz çiftliğinde belirlenmiş ve domuzlardan insana bulaştığı tespit edilmiştir. Dünya Sağlık Örgütü, virüsün kıtalar arasında hızlı yayılım göstermesinden dolayı ismini “Pandemik grip” olarak teyit etmiştir. Altı aydan 24 yaşa kadar olan kişiler, yaşlılar ve kronik hastalığı olanlar ile sağlık personell...

Sakiz (Chios) sheep is located in Mediterranean and Aegean costs of Turkey and characterized by high litter size. Previous studies have suggested that fertility characteristics of Sakiz sheep may be controlled by an unidentified a major F gene. Bone morphogenetic protein 15 (BMP15) and bone morphogenetic protein receptor-1B (BMPR-1B) genes were ide...

Aim: This study was carried out to determine some production characteristics of F1 and F2 Denizli X Leghorn populations including hatching weight, growth performance, live weights of the growing period, egg gravity and egg weight. Material and Methods: Ten Denizli roosters and 30 Leghorn hens were used for generation of the base population. In the...

Çalışmada, süt yağı ve süt verimi üzerine etkili olan diacyleglycerol-acyltransferase 1 (DGAT1) geni K232A polimorfizmi ve süt bileşimine etki ettiği düşünülen prion protein (PRNP) geni promotor ile intron 1 bölgelerinde indel polimorfizmleri incelenmiştir. Süt yağının neredeyse tamamını oluşturan trigliserid sentezini katalizleyen DGAT1 geni süt v...

Mitokondrial DNA (mtDNA) türlerin coğrafi dağılımına göre farklılıklar göstermesi, genomik DNA‘ya oranla daha hızlı evrimleşmesi, rekombinasyon olmaması ve maternal kalıtım göstermesi gibi özelliklerinden dolayı evcil hayvan populasyonlarının filogenetik çalışmalarında sıklıkla tercih edilen belirteçlerden birisidir. Bu çalışmanın amacı, Türkiye'de...

Giriş-Amaç: Vatandaşın yaşam hakkının ve sağlıklı bir hayat sürdürebilmesinin güvence altına alınması sosyal devlet anlayışının temel göstergelerinden bir tanesidir. Sağlıkta Dönüşüm Programı’nın amaçları; sağlık hizmetlerinin etkili, verimli ve hakkaniyete uygun bir şekilde organize edilmesi, finansmanının sağlanması ve sunulmasıdır. Bu çalışmada,...

GİRİŞ/AMAÇ: Sağlıklı bir toplum oluşturulması ve/veya mevcut sağlıklı ortamın devam ettirilmesi hasta ile hekim arasında kurulabilecek ilişkilerin niteliğine bağlıdır. Hastanın hekim ile kuracağı ilişkide hastane ortamında karşılaşacağı problemler önemli yer tutmaktadır. Hasta hakları; Sağlık hizmetlerinden faydalanma ihtiyacı bulunan fertlerin, sı...

Bu araştırma, Denizli x Leghorn F1 ve F2 populasyonun çıkım ağırlığı, büyüme ve verim dönemine ait canlı ağırlıkları, yumurta ağırlığı ve spesifik gravitelerinin belirlenmesi amaçlamıştır. Temel populasyonda 10 Denizli horozu ve 30 Leghorn tavuk kullanılmıştır. İlk generasyonda (F1), 15 erkek, 68 dişi ile elde edilmiştir. İkinci generasyonda ise 47...

Mitochondrial DNA D-loop region is well known as the most evolving segment. Sequence polymorphism of mtDNA D-loop region is widely used for dissemination of genetic diversity in various animal populations. Objective of this study is to determine genetic diversity and phylogenetic relationship of six native cattle breeds of Turkey as part of TUBITAK...

As part of a national project titled “In vitro Conservation and Preliminary Molecular Identification of Some Turkish Domestic Animal Genetic Resources-I (TURKHAYGEN-I)”, genetic structure, evolutionary relationships and genetic diversities were investigated among six local cattle breeds in Turkey. A total of 246 blood samples were collected from An...

Kalıtım derecesi ve damızlık değerlerinin hesaplanması, hayvanın gerçek değerinin tespit edilmesi ile adli tıp çalışmalarında, bir populasyonda bulunan hayvanların genetik ilişkilerinin doğru olarak belirlenmesinin önemi büyüktür. Son zamanlarda, moleküler düzeyde genetik karakterizasyon verileri kullanılarak ırk tespiti çalışmaları yapılmaktadır....

Değişen çevre koşulları dünya fauna ve florasını olumsuz yönde etkilemektedir. Bu çalışmanın amacı, Türkiye Yerli Evcil Genetik Kaynaklarından Bazılarının in vitro Korunması ve Ön Moleküler Tanımlanması- I (TÜRKHAYGEN-I) projesi kapsamında, Türkiye’de bulunan yerli sığır ırklarının mikrosatellit DNA markörler kullanılarak genetik karakterizasyonu v...

In this study, five different isolation protocols to extract total RNA from biopsies of equine endometrium were compared in terms of quality and quantity of RNA samples with respect to downstream gene transcription analysis, such as Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Three phenol-chloroform based protocols (TRIzol, TRItidy, E...

In this study, five different isolation protocols to extract total RNA from biopsies of equine endometrium were compared in terms of quality and quantity of RNA samples with respect to downstream gene transcription analysis, such as Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Three phenol-chloroform based protocols (TRIzol, TRItidy, E...

Değişen çevre koşulları dünya fauna ve florasını olumsuz yönde etkilemektedir. Bu çalışmanın amacı, Türkiye Yerli Evcil Genetik Kaynaklarından Bazılarının İn Vitro Korunması ve Ön Moleküler Tanımlanması- I (TÜRKHAYGEN-I) projesi kapsamında, Türkiye’de bulunan yerli sığır ırklarının mikrosatellit markörler kullanılarak genetik karakterizayonu ve fil...

In this study, a F2 level Denizli X White Leghorn population (n = 441) was used to dissect chromosomal regions, which have an affect on body weight and egg yield. DNA samples were isolated from all F0, F1 and F2 animals using a standard phenol/chloroform method. For chromosomal level screening, a total of 99 markers which were suitable for genome s...

Development of gene maps for human and animals is critical for identification of genomic regions and gene(s) that have an effect on human and animal diseases and animal production traits. Gene level dissection of diseases and these traits allows diagnosis, protection of diseases and development of novel medical treatments. The genetic level knowled...

Bu çalışmanın amacı, beş farklı RNA izolasyon metodu kullanılarak elde edilen total RNA’nın kalite ve miktarlarının reverz transkripsiyon-polimeraz zincir reaksiyonununda (RT-PZR) kullanılabilirliğinin araştırılmasıdır. Ticari olarak mevcut fenol/kloroform esasına dayanan üç farklı izolasyon kiti (Trizol, Tritidy, EZ-RNA) ve kolon esasına dayanan i...

Populasyonların moleküler düzeyde karakterizasyonu, ırk içi ve ırklar arası genetik çeşitliliğin tespiti ile ırkların birbirleriyle olan genetik uzaklıklarının belirlenmesine olanak sağlamaktadır. Bu amaçla mikrosatellit DNA markörleri yaygın olarak kullanılmaktadır. DNA seviyesinde elde edilen genotipik veriler, bir populasyonu oluşturan bireyleri...

Microsatellites are one of the favored markers used in population genetics studies because of their high heterozygosity and versatility. In this study, 11 microsatellites loci were used as molecular markers to determine the intra and inter genetic diversities of eight different goat breeds including Kil, Angora, Kilis, Yayladag, Shami, Honamli, Saa...

At yetistiriciliginde, hayvanin gerçek degerinin belirlenmesi, soy kütügü kayitlari, bilimsel çalismalar ve adli olaylarda ebeveyn ve birey tespiti önem arz etmektedir. Bu amaçla DNA teknolojisi yaygin olarak kullanilmaktadir. Sunulan bu çalismanin amaci, T.C. Kars Cumhuriyet Bassavciligi Hazirlik Bürosu tarafindan gönderilen kisrak at ve yavru ata...

Introduction: Genetic characterization of populations and thereby determination of diversity are critically important for development of management programs for genetic resources and determination of population migrations in the history. Methods: Ninety six of animals of cat populations, Turkish Van, Turkish Angora, Turkish Tekir, Siamese and Persi...