Yuriy V Shatalin

• MS, PhD
• Senior researcher at Russian Academy of Sciences

The aim of this study was to evaluate the kinetic parameters of the oxidation of some 3-aminopyridine-2(1H)-ones by hydrogen peroxide catalyzed by horseradish peroxidase and the affinity of horseradish peroxidase to these compounds. It has been shown that the oxidation of 3-aminopyridine-2(1H)-ones follows kinetics of the pseudo-first order. A hype...

This review is devoted to modern concepts of paraptosis as one of the types of regulated cell death, in comparison with other types of cell death. Paraptosis is a form of cell death caused by stress of the endoplasmic reticulum (ER), accompanied by the accumulation of damaged or incorrectly folded proteins in it, extensive nonautophagic vacuolation...

Previously, we showed that taxifolin derivatives, taxifolin pentaglutarate and a conjugate of taxifolin with glyoxylic acid, improve the mechanical properties of collagen-based materials. In the process of degradation of such materials, biologically active polyphenols are released into the surrounding medium. Two approaches were used to assess the...

The aim of the present work was to estimate the kinetic parameters of oxidation of some 3-aminopyridin-2(1H)-ones by hydrogen peroxide catalyzed by horseradish peroxidase and the affinity of horseradish peroxidase towards these compounds. It was shown that the oxidation of 3-aminopyridin-2(1H)-ones follows pseudo-first-order kinetics. It was also f...

The review is devoted to modern ideas about paraptosis, as one of the types of regulated cell death, in comparison with other types of cell death. Paraptosis is a form of cell death caused by endoplasmic reticulum stress, accompanied by an accumulation of damaged or misfolded proteins, extensive non-autophagic vacuolation of cisterns of endoplasmic...

Our previous research has shown that derivatives of taxifolin, pentaglutarate of taxifolin and a conjugate of taxifolin with glyoxalic acid improve the mechanical properties of the collagen-based materials. During the degradation process of these materials, the biologically active polyphenols are released into the surrounding medium. To evaluate th...

Two novel membranes based on collagen and two polyphenols, taxifolin pentaglutarate (TfG5) and a conjugate of taxifolin with glyoxylic acid (DfTf), were prepared. Fourier transform infrared spectroscopy examination confirmed the preservation of the triple helical structure of collagen. A scanning electron microscopy study showed that both materials...

Disulfiram (DSF) and its oxidized derivatives (DSFoxy) are currently being investigated as possible anticancer agents. We previously found that DSFoxy initiate paraptosis-like death of tumor cells, which is of potential interest for the treatment of tumors resistant to the initiation of apoptosis. Based on bioinformatics analysis of mass spectromet...

In this work, the properties of gel materials based on collagen and derivatives of taxifolin, pentaglutarate of taxifolin, and conjugate of taxifolin with glyoxylic acid were studied. It was shown that the increase in the proportion of the polyphenols in a gel led to the decrease in the rate of degradation of the materials. The materials had no neg...

Polyphenols are secondary metabolites of plants. These compounds are widely distributed in nature and present in human diets.The consumption of polyphenol-rich foods and beverages may be associated with a reduction of the risk of cardiovascular, neurodegenerative, and some other diseases. During the storage, processing, and digestion of polyphenol-...

Vitamin B12, or cobalamin, is essential for normal body function and used in the therapy of different diseases. A several studies have shown that vitamin B12 has anti-inflammatory and antioxidant properties that can play an important role in the prevention of some diseases. On the other hand, it has been reported that vitamin B12 in combination wit...

The aim of the present work was to study the effects of taxifolin, a conjugate of taxifolin with glyoxylic acid, and naringenin on phagocytosis of latex beads by neutrophils and adhesive properties of these cells. It was shown that taxifolin had no effect on the phagocytic activity and adhesive properties of neutrophils. A conjugate of taxifolin wi...

During the storage, processing, and digestion of flavonoid-rich foods and beverages, a condensation of flavonoids with toxic carbonyl compounds occurs. The effect of the resulting products on cells remains largely unknown. The aim of the present study was to evaluate the effects of quercetin, taxifolin, catechin, eriodictyol, hesperetin, naringenin...

Dear Colleagues, Polyphenols are secondary metabolites in plants. These compounds are found in plant-based foods and beverages and are an integral part of our diet. The consumption of polyphenols or their sources may be associated with a reduced risk of a number of diseases, including cardiovascular, neurodegenerative and other diseases. These com...

Turpentine oil, owing to the presence of 7–50 terpenes, has analgesic, anti-inflammatory, immunomodulatory, antibacterial, anticoagulant, antioxidant, and antitumor properties, which are important for medical emulsion preparation. The addition of turpentine oil to squalene emulsions can increase their effectiveness, thereby reducing the concentrati...

Cobalamin is an essential nutrient required for the normal functioning of cells. Its deficiency can lead to various pathological states. Hydroxocobalamin (HOCbl) and cyanocobalamin (CNCbl) are the forms of vitamin B12 that are most commonly used for supplementation. There is substantial evidence indicating that cobalamins can both suppress and prom...

Disulfiram (DSF) and its derivatives were here investigated as antineoplastic agents, and their important feature is the ability to influence the UPS. We have recently shown that hydroxocobalamin catalyzes the aerobic oxidation of diethyldithiocarbamate to form disulfiram and its oxy-derivatives (DSFoxy; i.e., sulfones and sulfoxides), which induce...

Cobalamin is an essential nutrient required for the normal functioning of cells. Its deficiency can lead to various pathological states. Hydroxocobalamin (HOCbl) and cyanocobalamin (CNCbl) are the forms of vitamin B12 that are most commonly used for supplementation. There is substantial evidence indicating that cobalamins can both suppress and prom...

Background Dithiocarbamates and derivatives (including disulfiram, DSF) are currently investigated as antineoplastic agents. We have revealed earlier the ability of hydroxocobalamin (vitamin В12b) combined with diethyldithiocarbamate (DDC) to catalyze the formation of highly cytotoxic oxidized derivatives of DSF (DSFoxy, sulfones and sulfoxides)....

Reduction of benzo[c] [1,7]naphthyridin-4(3H)-ones with sodium borohydride in acetic acid led to a series of 5-ethyl-5,6-dihydrobenzo[c] [1,7]naphthyridin-4(3H)-ones. The latter are photostable fluorophores emitting in the green region of the visible spectrum with a fluorescence quantum yield of up to 0.43 and a Stokes shift of up to 133 nm, contai...

It is known that flavonoids can react with toxic carbonyl compounds in the process of the storage, aging, and digestion of flavonoid-rich foods and beverages. However, the effect of these reactions on the antioxidant properties of the polyphenolic fraction and the properties of the resulting products remain poorly studied. The aim of the present wo...

A series of new 3-amino-4-arylpyridin-2(1H)-one derivatives was synthesized on the basis of oxazolo[5,4-b]pyridin-2(1H)-ones. Their photophysical properties and antioxidant activity were studied. Effective luminophores with an photoluminescence yield of up to 0.76 and substances exhibiting antioxidant activity exceeding ascorbic acid were revealed....

We have shown that hydroxycobalamin (vitamin B12b) increases the toxicity of diethyldithiocarbamate (DDC) to tumor cells by catalyzing the formation of disulfiram (DSF) oxi-derivatives. The purpose of this study was to elucidate the mechanism of tumor cell death induced by the combination DDC + B12b. It was found that cell death induced by DDC + B1...

The properties of gels prepared from collagen and the natural polyphenol taxifolin were studied. The degradation rate of the gel was shown to decrease with increasing fraction of functionalized taxifolin in it. Gel including polyphenol exhibited iron(II)-binding and iron(III)-reducing activity whereas polyphenol released from the gel could reduce i...

It is known that some metals (Cu, Zn, Cd, Au) markedly increase the toxic effect of thiocarbamates. It was shown in the present study that hydroxycobalamin (a form of vitamin B12, HOCbl), which incorporates cobalt, significantly enhances the cytotoxicity of diethyldithiocarbamate (DDC), decreasing its IC50 value in tumor cells three to five times....

Bone tissue engineering is considered an alternative approach for conventional strategies available to treat bone defects. In this study, we have developed bone scaffolds composed of hydroxyapaptite (HAp), gelatin and mesoporous silica, all recognized as promising materials in bone tissue engineering due to favorable biocompatibility, osteoconducti...

The interaction of two structurally close flavanones: taxifolin and naringenin with copper(I) ions and its effect on the distribution of flavonoids and the corresponding ions in a biphasic system octanol–water have been studied. It has been shown that these polyphenols form complexes with copper ions of different stoichiometric ratio depending on t...

The condensation of taxifolin with glyoxylic acid was examined, and the properties of the resulting product were compared with those of taxifolin. The structure of the product was determined by NMR spectroscopy. The ability of the polyphenols to scavenge reactive oxygen species (ROS) was estimated by luminol-dependent chemiluminescence. The iron-ch...

The possibility of producing the material based on collagen and biologically active polyphenol taxifolin was explored, and the properties of the material were studied. The data on the dynamics of the release of polyphenol chemically linked to collagen are represented, and the metal-reducing activity of polyphenol released from the gel is determined...

The possibility of producing a material based on collagen and a biologically active polyphenol, viz., taxifolin, was investigated, and the properties of the material were studied. The data on the release dynamics and metal-reducing activity of polyphenol that is chemically linked to collagen are represented. The effects of taxifolin, taxifolin glut...

Using a spectrophotometric method, changes occurring in solution containing brain Aβ(1-42)-peptide, fullerene C60, and polyvinylpyrrolidone were analyzed. Using the Bent-French method, relative binding constants of fullerene C60 with Aβ(1-42)-peptide and polyvinylpyrrolidone with Aβ(1-42)-peptide were determined. These data suggest that Aβ(1-42)-pe...

Using a spectrophotometric method, changes occurring in solution containing brain Aβ(1–42)-peptide, fullerene C60, and polyvinylpyrrolidone were analyzed. Using the Bent–French method, relative binding constants of fullerene C60 with Aβ(1–42)-peptide and polyvinylpyrrolidone with Aβ(1–42)-peptide were determined. These data suggest that Aβ(1–42)-pe...

The composition of taxifolin-iron ions complexes in an octanol-water biphasic system was studied using the method of absorption spectrophotometry. It was found that at pH 5.0 in an aqueous biphasic system the complex of [Tf · Fe2(OH) k (H2O)8 − k ] is present, but at pH 7.0 and 9.0 the complexes of [Tf2 · Fe(OH) k (H2O)2 − k ] and [Tf · Fe(OH) k (H...

We have used absorption spectroscopy to study the acid-base and metal-binding properties of two structurally similar flavanones: taxifolin and naringenin. We have determined the acid dissociation constants for taxifolin (pKa1 = 7.10 ± 0.05, pKa2 = 8.60 ± 0.09, pKa3 = 8.59 ± 0.19, pKa4 = 11.82 ± 0.36) and naringenin (pKa1 = 7.05 ± 0.05, pKa2 = 8.85...

In this work, the effect of tumor growth on the antioxidant system was studied in blood plasma and the tumor growth zone. As a model system of tumor growth, we used Zajdela ascitic hepatoma transplanted into the abdominal cavity of Wistar rats. The tumor produced a disbalance between pro- and antioxidant systems in the body of the tumor bearer. In...

We studied the effect of liposome preparation containing taxifolin oligomers and taxifolin conjugates with carbonyl compounds on skin regeneration after chemical burn. The preparations containing flavonoid conjugates intensified regeneration processes and reparation of hair follicles and sebaceous glands after chemical burn. The preparation based o...

The effect of the liposomal form formed by taxifolin and metals of variable valency was investigated. It was shown that application of preparations based on the free flavonoid and its complexes with Fe(II/III) and Cu(II) ions after chemical burns results in more effective skin regeneration and repair of hair follicles and sebaceous glands. A tenden...

The effect of the liposomal form formed by taxifolin and metals of variable valency was investigated. It was shown that the application of preparations based on the free flavonoid and its complexes with Fe(II/III) and Cu(II) ions after chemical burns results in a more effective skin regeneration and the repair of hair follicles and sebaceous glands...

The probe 3,7-dihydro-2-methyl-6-(4-methoxyphenyl)imidazol[1,2-a]pyrazine-3-one (MCLA) is widely used for studying the superoxide anion production and the efficiency of antioxidants in biological systems. Here we report that a number of sulfur-containing compounds applied in biochemical and cytological studies are able to suppress MCLA-derived chem...

Dihydroquercetin (flavonoid of plant origin) immobilized with an amino acid in lecithin nanoparticles promotes reduction of inflammatory reactions in the wound after thermal burn. The use of a liposomal complex in burn injury stabilizes endogenous antioxidant system and limits the secondary necrotic zone in the wounds. The treatment was associated...

The action of six synthetic Biginelli pyrimidines on the production of reactive oxygen species (ROS) by polymorphonuclear leukocytes has been studied. It has been shown using the method of luminoldependent chemiluminescence that, at concentrations of 10–100 μM, these compounds stimulate the production of ROS by neutrophils stimulated by phorbol-12-...

The ability of the flavonoid dihydroquercetin to prevent or accelerate the accumulation of reactive oxygen species and the metabolites of oxidative stress, carbonyl compounds has been studied. It has been shown on a model of oxidation of lecithin that dihydroquercetin exhibits a prooxidant effect in the alkaline region of pH, whereas at neutral and...

Using Seidel ascites hepatoma as a model, we studied in detail changes in cell population size and in the level of reactive oxygen species in the tumor growth zone and in the blood plasma of tumor carrier. It was found that reduction-oxidation conditions in the blood plasma and in the tumor growth zone were different. Thus, because of hyperactivity...

Liposomes containing natural flavonoid dihydroquercetin, phospholipid lecithin, and zwitterionic amino acid glycine were used for the therapy of glacial acetic acid-induced chemical burn. No inflammatory reaction was observed in the region of wound. The area of postburn injury did not increase in time. Planimetry and histological studies showed tha...

The behavioral effects of 3,4-dihydro-2(1H)-pyrimidinethione (DPT, a pyrimidine derivative), which is used as a test system for detecting tumor growth, on the ultrastructure and function of Mauthner neurons (MN), were studied in goldfish. Application of DPT to MN was found to lead to increased resistance of neurons to exhaustive stimulation, which...

The antioxidant activity of duroquinone and hypoxen was compared with that of alpha-tocopherol and vitamin C in a model system (luminol-peroxidase-H2O2), and their influence on the level of reactive oxygen species in systems containing polymorphonuclear leukocytes of healthy and tumor-bearing animals was studied. It was shown that, in a model chemi...

The effects of pyrimidine derivative 3,4-dihydro-2(1H)-pyrimidinethione, (DPT) used as a test-system for detection of tumor growth, on the goldfish Mauthner neurons (MN) ultrastructure and function, as manifested in behavioral changes, were studied. The results of investigations demonstrated that an application of DPT on MN had the effects similar...

Amyloid oligomers, protofibrils, and fibrils of various amyloidogenic proteins are known to induce cell death. Tetracycline prevents the formation of fibrils of Aβ peptide and other amyloidogenic proteins and decomposes mature fibrils. It was previously shown that sarcomeric cytoskeletal proteins of the titin family (protein X, protein C, and prote...

It is known that amyloid oligomers, protofibrils, and fibrils induce cell death, and antibiotic tetracycline inhibits the fibrillization of beta amyloid peptides and other amyloidogenic proteins and disassembles their pre-formed fibrils. Earlier we have demonstrated that sarcomeric cytoskeletal proteins of the titin family (X-, C-, and H-proteins)...

Luminescence and absorption stains specific for DNA (acridine orange, ethidium bromide), proteins (silver nitrate), and lipids (Sudan III) were used to study the distribution of DNA, proteins, and lipids in sections of the olfactory bulb in rats, studies being performed after fixation of brains with paraformaldehyde. DNA was found to be more abunda...

A vast number of studies, including the authors' own research, support the important role polymorphonuclear leukocytes (PMNL) in the development of ascite tumors. The method of luminol-dependent chemiluminescence (CL) was used to show the presence of two functionally different PMNL pools in a tumor-bearing organism: 1) "primed" PMNL, which circulat...

Using luminescent and absorption stains, specific for DNA (acridine orange, ethidium bromide), proteins (silver nitrate) and lipids (sudan III), the distribution of these substances was studied in the sections of rat olfactory bulbs, fixed by paraformaldehyde. DNA prevalence was found in glomerular cell layer as compared with the mitral one. Large...