Yuqin Wu

Yuqin Wu
Monash University (Australia) · Faculty of Medicine, Nursing and Health Sciences

PHD candidate

About

24
Publications
3,157
Reads
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110
Citations
Introduction
Yuqin Wu currently studies at the Monash Biomedicine Discovery Institute, Monash University. His PhD project is to explore novel aspects of glucagon biology.

Publications

Publications (24)
Article
This study was conducted to investigate the effects of stocking density and dietary nicotinamide (NAM) and butyrate sodium (BA) supplementation on the growth performance, liver mitochondrial function and gut microbiota of broilers under high stocking density. A total of 342, 26-d-old Cobb500 broilers were divided into 5 groups with 6 replicates. Tr...
Article
Full-text available
This study aimed to determine the impact of stocking density on the liver proteome and cecal microbiota of Peking ducks. A total of 1,200 21-day-old ducks were randomly assigned to 5 stocking density groups of 5, 6, 7, 8 and 9 ducks/m², with 6 replicates for each group. At 40 days of age, duck serum and pectorals were collected for biochemical test...
Article
Full-text available
Fasting is typically used to empty the gastrointestinal tract (GIT) and assess feed metabolizable energy (ME). However, the effects of fasting on energy and nutrient utilization is not well understood. This study aimed to explore the difference in GIT emptying, energy and nutrient utilization of broilers and adult roosters fed corn-soybean meal-bas...
Article
Full-text available
Background The use of newly harvested corn in feed causes wet droppings in broilers and increased feed cost which was termed as “new season grain problem”. The present study was conducted to evaluate the proteomic profile of newly harvested corn and the subsequent influence on intestinal microbiol community for broiler chickens. Methods Newly harv...
Article
Full-text available
This study was conducted to investigate the effects of naturally oxidized corn oil on the inflammatory reaction and intestinal health of broilers. Total 450, 1-day-old Arbor Acres male broilers were randomly divided into five treatments with 6 replicate cages (15 birds in each replicate cage). The dietary treatment array consisted of the varying ra...
Article
Full-text available
Methionine and its hydroxy analogue (MHA) have been shown to benefit mouse intestinal regeneration. The intestinal organoid is a good model that directly reflects the impact of certain nutrients or chemicals on intestinal development. Here, we aimed to establish a chicken intestinal organoid culture method first and then use the model to explore th...
Article
Full-text available
Fasting-induced autophagy in the intestine is beneficial for body health. This study was designed to explore the relationship between the host metabolism and intestinal autophagy. Broilers were randomly assigned into 48 cages. At 0 (CT), 12 (FH12), 24 (FH24), 36 (FH36), 48(FH48), and 72 h (FH72) before 09:00 a.m. on day 25, eight cages of birds wer...
Preprint
Full-text available
Background The use of newly harvested corn in feed causes wet droppings in broilers and increased feed cost which was termed as “new season grain problem”. The present study was conducted to evaluate the proteomic profile of newly harvested corn and the subsequent influence on intestinal microbiol community for broiler chickens. Methods Newly harve...
Article
Full-text available
Xylan oligosaccharides (XOS) can promote proliferation of Pediococcus acidilactic BCC-1, which benefits gut health and growth performance of broilers. The study aimed to investigate the effect of Pediococcus acidilactic BCC-1 (referred to BBC) and XOS on the gut metabolome and microbiota of broilers. The feed conversion ratio of BBC group, XOS grou...
Article
Full-text available
Both obesity and sarcopenia are frequently associated in ageing, and together may promote the progression of related conditions such as diabetes and frailty. However, little is known about the pathophysiological mechanisms underpinning this association. Here we show that systemic alanine metabolism is linked to glycaemic control. We find that expre...
Article
Full-text available
Healthy diets are necessary for both humans and animals, including poultry. These diets contain various nutrients for maintenance and production in laying hens. Therefore, research was undertaken to explore the efficiency of various dietary flaxseed sources on the n-3 deposition in the egg yolk and gene expression in laying hens. Five dietary group...
Article
Food withdrawal is usually used for accurate feed metabolizable energy (ME) assessment in poultry, but its effects on intestinal structure and the absorption of nutrients are unclear. In this study, broilers were fed ad libitum (CT) or withdrew food for 12 (FH12), 24 (FH24), 36 (FH36), or 48 hours (FH48). We showed that food withdrawal increased th...
Article
Full-text available
Background In recent years, increased attention has been focused on breast muscle yield and meat quality in poultry production. Supplementation with nicotinamide and butyrate sodium can improve the meat quality of broilers. However, the potential molecular mechanism is not clear yet. This study was designed to investigate the effects of supplementa...
Article
This study was aimed to explore the comparative acidifying properties of 2‐hydroxy‐4‐(methylthio) butanoic acid (HMTBA) and a combination of DL‐methionine (DLM) and acidifier in male broiler production. A total of 480 1‐day‐old broiler chicks were randomly divided into four treatments: A (low HMTBA, 0.057% HMTBA); B (low acidifier, 0.05% DLM + 0.05...
Article
Full-text available
Background: Polyunsaturated fatty acids (PUFA), particularly n-3, have beneficial effects on human health, and for this reason foodstuffs with increased content of n-3 PUFA are now very common and widely available. Design: This study was conducted to investigate the effect of the duration of a flaxseed diet on Peking duck's growth performance, a...
Article
Full-text available
This study was conducted to investigate the effects of supplementation with nicotinamide (NAM) and sodium butyrate (BA) on meat quality and expression of muscle development genes in broilers reared at a high stocking density. A total of 567, 21-day-old AA broilers were randomly assigned to 5 treatment groups and 2 control groups, with 7 replicates...
Preprint
Background In recent years, increased attention has been focused on breast muscle yield and meat quality in poultry production. Supplementation with nicotinamide and butyrate sodium can improve the meat quality of broilers. However, the potential molecular mechanism is not clear yet. This study was designed to investigate the effects of supplementa...
Preprint
Full-text available
Background In recent years, increased attention has been focused on breast muscle yield and meat quality in poultry production. Supplementation with nicotinamide and butyrate sodium can improve the meat quality of broilers. However, the potential molecular mechanism is not clear yet. This study was designed to investigate the effects of supplementa...
Preprint
Full-text available
Background: In recent years, increased attention has been focused on breast muscle yield and meat quality in poultry production. Supplementation with nicotinamide and butyrate sodium can improve the meat quality of broilers. However, the potential molecular mechanism is not clear yet. This study was designed to investigate the effects of supplement...
Preprint
Full-text available
Background: In recent years, increased attention has been focused on breast muscle yield and meat quality in poultry production. Supplementation with nicotinamide and butyrate sodium can improve the meat quality of broilers. However, the potential molecular mechanism is not clear yet. This study was designed to investigate the effects of supplement...
Article
To study why flaxseed supplementation causes adverse effects on the performance of poultry, we investigated the gut microbiota of Peking ducks after consumption of a flaxseed diet. A total of 792, 12-day-old white Peking ducks were divided into four groups. In the control group, birds were provided with a basal diet. In the three experimental group...
Article
Full-text available
This study aimed to determine the dietary energy and protein levels for optimal growth performance and carcass characters of free force-feeding Peking ducks from 21 to 38 days of age. A total of 1,440 21-d-old Peking ducks were randomly assigned to 4 dietary metabolic energy (ME) and crude protein (CP) treatments: low nutrient (ME 11.93 MJ/kg, CP 1...
Preprint
Full-text available
The aim of this study was to determine the impact of stocking density on the liver proteome and cecal microbiota of Peking ducks. A total of 1,200 ducks with 21-day old were randomly allotted into 5 stocking density groups of 5, 6, 7, 8 and 9 ducks/m2, with 6 replicates for each group. At 40 days of age, duck serum and pectorals were collected for...
Article
Full-text available
Xylanoligosaccharides (XOS) is the hydrolysates of xylan. To compare the proliferation effect of XOS, glucose, fructooligosaccharides (FOS), xylose, XOS and a media without carbohydrate source (control) on Pediococcus acidilactici strain BCC-1. The de-novo sequencing of Pediococcus acidilactici strain BCC-1 was conducted, and the underlying mechani...

Questions

Question (1)
Question
I want to do tissue subcellular fractionation of mouse liver. According to the method, the speed to separate ER is over 200,000g for 120 min (Song et al., 2006). However, liver exosome isolation only needs 100,000 x g for 70 min (Ishiguro et al., 2019). Thus, I'm wondering how to separate exosome and ER?
Song, Y., Hao, Y., Sun, A., Li, T., Li, W., Guo, L., ... & Wei, H. (2006). Sample preparation project for the subcellular proteome of mouse liver. Proteomics, 6(19), 5269-5277.
Ishiguro, K., Yan, I. K., & Patel, T. (2019). Isolation of tissue extracellular vesicles from the Liver. JoVE (Journal of Visualized Experiments), (150), e58649.

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