Yumiko Ishii

Yumiko Ishii
The University of Tokyo | Todai · Institute of Medical Science

About

55
Publications
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1,108
Citations

Publications

Publications (55)
Article
Full-text available
Round spermatid injection (ROSI) into unfertilized oocytes enables a male with a severe spermatogenesis disorder to have children. One limitation of the application of this technique in the clinic is the identification and isolation of round spermatids from testis tissue. Here we developed an efficient and simple method to isolate rodent haploid ro...
Article
While the role of p75(NTR) signaling in the regulation of nerve-related cell growth and survival has been well documented, its actions in osteoblasts are poorly understood. In this study, we examined the effects of p75(NTR) on osteoblast proliferation and differentiation using the MC3T3-E1 pre-osteoblast cell line. Proliferation and osteogenic diff...
Article
We describe a novel role for CD271 in the differentiation of mesenchymal stem cells (MSCs), including deciduous dental pulp stem cells (DDPSCs) and murine multipotent MSCs (C3H10T1/2 cells). The CD271(+) subpopulation of deciduous dental pulp cells (CD271(+)/DDPSCs) and the forced expression of CD271 in C3H10T1/2 (10T271) were analyzed by fluoresce...
Article
Although disturbed cytokinesis of mononuclear Hodgkin (H) cells is thought to generate Reed-Sternberg (RS) cells, differentiation of Hodgkin's lymphoma (HL) cells is not fully understood. Recent studies indicate that cells found in a side population (SP) share characteristics of cancer stem cells. In this study we identified an SP in the HL cell li...
Article
Recent studies have demonstrated that human dental pulp contains adult stem cells. A pulse of the thymidine analog BrdU given to young animals at the optimal time could clarify where slow-cycling long-term label-retaining cells (LRCs), putative adult stem cells, reside in the pulp tissue. This study focuses on the mapping of LRCs in growing teeth a...
Article
Although there are numerous reports describing the in vivo bone forming capability of recombinant human bone morphogenetic proteins-2 (rhBMP-2), studies have reported limited effects on human mesenchymal stem cells (hMSCs). However, the reasons for these discrepancies are not well understood. The aim of this study was to investigate the responsiven...
Article
Full-text available
Although human bone marrow stromal cells (BMSCs) have the ability to form bone when transplanted, the responsible factors for in vivo osteogenic abilities are poorly understood. Here we report conditions that are required for human BMSCs to demonstrate their in vivo osteogenic abilities. BMSCs were obtained from healthy donors and their in vivo ost...
Article
Current standard techniques for bone tissue engineering utilize ex vivo expanded osteogenic cells. However, ex vivo expansion requires serum, which may hinder clinical applications. Here, we report the feasibility and efficacy of bone tissue engineering with human bone marrow stromal cells (BMSCs) expanded in serum-free conditions. Bone marrow was...
Article
That generation of allele-specific anti-human leukocyte antigen (HLA) monoclonal antibodies (ASHmAb) is very difficult is well known. This is thought to be due to the unique epitope structure, an assemblage of amino acid residues that lie separately in the amino acid sequence of human HLA, and to its low antigenicity compared with that of common ep...
Article
Full-text available
Although umbilical cord blood has been increasingly used as an alternative donor source to treat hematologic malignancies, cord blood transplantation (CBT) is frequently complicated by graft failure and relapse of primary diseases. Because persistence or increase of recipient-derived hematopoietic or malignant cells has pathogenic import under thes...
Article
Full-text available
Here we report a new anti-rat Thy-1 monoclonal antibody (MAb) 12C5 that recognized a novel epitope on a GPI-anchored Thy-1 antigen. In rat thymocytes, MAb 12C5 reacted with 25 kDa Thy-1 antigen purified with MAb OX7. In rats, MAb 12C5 was expressed on 94% of thymocytes, 46% of spleen cells, 31% of mesenteric lymphnode cells, 76% of bone marrow cell...
Article
This report describes the production and characterization of monoclonal antibody specific for an antigenic determinant, designated Y1 antigen, present on Jurkat cells. Mo-Y1 specifically reacts with Jurkat cells, but not with other cell lines. Immunoprecipitation and subsequent immunochemical analysis demonstrate that Mo-Y1 defines an antigen with...
Article
We have generated a battery of monoclonal antibodies (MAbs) that distinguish different antigen molecules present in human B cells. Two antigen systems, termed L26 and L27, respectively, were expressed in most surface immunoglobulin-positive B cells and thus showed pan-B cell specificity. Two other antigens (L22 and L30) appeared to be expressed mai...
Article
Con A-activated rat thymocytes were used to immunize mice, and immune spleen cells were fused with NS/1 myeloma cells. One clone, designated 5C6-F4, reacted strongly with Con A-activated rat thymocytes and some LPS-activated rat spleen cells but not with normal thymocytes, spleen cells, or bone marrow cells of rat origin. The 5C6-F4 did not react w...
Article
Two antigen systems (L29 & L30) expressed on two distinct human B cell subpopulations were identified by using BL1-4D6 and TB3-7D5 monoclonal antibodies, respectively. L29 was expressed on approximately one-third of B cells in human lymphoid tissues. These B cells associated with L29 were large activated B cells located in the germinal centres of l...
Article
The tumor-specific transplantation antigens (TSTAs) of BALB/c mouse colon tumor C-C36 were characterized by gel filtration (TKS-gel FPLC), ion exchange chromatography (Mono Q FPLC) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The cell surface antigens were solubilized noncytolytically with 2.5% n-butanol. It wa...
Article
Distributions and ultrastructures of T cell subsets within grafts were studied in unmodified, first-set rat renal transplantation systems (F344----WKA, TO----WKA). Rejections took place within 9.0 +/- 0.5 and 12.2 +/- 1.6 days, respectively. Mouse monoclonal antibodies, R1-3B3 and R1-10B5, were used here for the detection of RLyt-1 and RLyt-2 antig...
Article
A novel cell-surface antigen (L25) expressed on human B cells was identified using a B cell-reactive monoclonal antibody (TB1-4D5). This L25 antigen was expressed on most B-lineage cells but not other cell types including thymocytes, T cells, granulocytes and monocytes. Thus, L25 existed on the majority of normal B cells present in the blood and ly...
Article
Twenty-nine cases of non-Hodgkin's lymphoma of Waldeyer's ring (W-NHL) and nasal cavity or paranasal sinus (N-NHL) were studied for tumor-surface marker phenotype and histopathologic correlation with clinical features. Immunostaining procedures on tissue sections by using xenoantisera and monoclonal antibodies to human B- and T-cells enabled the au...
Article
Three novel antigen systems (L22, L23, and L24) expressed on human B cell subpopulations were identified by using TB1-2C3, TB1-2B3, and TB1-3C1 monoclonal antibodies, respectively. L22 was expressed on a minor subpopulation of B cells in human lymphoid tissues and in the peripheral blood. These B cells associated with L22 were resting small B cells...
Article
Two distinct antigen systems (L26 and L27) specifically expressed in human B lymphocytes were identified using TB2-2B3 (2B3) and T3-5B3 (5B3) monoclonal antibodies, respectively. Whereas L26 antigen defined by 2B3 were rarely expressed on the surface of B cells but abundant in the cytoplasm, 127 antigens detected by 5B3 was clearly expressed on the...
Article
Full-text available
Lymphoid cell subpopulations infiltrating into autografts of methylcholanthrene-induced sarcomas in rats immunized with autologous tumor cells were identified in terms of immunohistochemical and cytofluorographic techniques using various monoclonal antibodies raised against different classes of rat lymphohemopoietic cells. These antibodies included...
Article
Two distinct novel antigen systems preferentially expressed in rat granulocytes and macrophages were detected using two different monoclonal antibodies (R2-1A6 and R2-2B1). These two antibodies reacted with approximately 50% of rat bone marrow cells, most granulocytes, blood monocytes, alveolar macrophages and peptone-elicited peritoneal macrophage...
Article
Rat T lymphocyte antigens were defined by using two distinct monoclonal antibodies (R1-3B3 and R1-10B5). R1-3B3 antibody, when tested for its reactivity with rat lymphoid cells by immunofluorescence, stained almost all of thymus and T cells but not the majority of B cells and bone marrow cells. The antigen defined by R1-3B3 existed more abundantly...
Article
Quantitative determination of S-100 protein content on human melanoma tissues and in sera of melanoma patients was made possible through radioimmunoassay (RIA). Melanoma tissue extracts prepared from metastatic sites in both skin and lymph nodes contained 0.08-2.80% of S-100 out of the total extractable proteins, and it was also noticed that the tu...
Article
Human thymus and T cell antigens were identified by using four distinct monoclonal antibodies (MoAb), designated 2D5, 5B3, 7A5 and 9D4. 2D5 antibody reacted with most human thymocytes and a few peripheral lymphocytes as well as with a subpopulation (20%) of bone marrow cells, and precipitated a 45K molecular weight (mol. wt.) component from 125I-la...
Article
Rat thymus and T-cell antigens were defined by using two distinct monoclonal antibodies (R-1-3B3 and R-1-12C5). R-1-3B3 antibody, when tested for its reactivity with rat lymphoid cells by immunofluorescence, labelled virtually all thymus and T cells but not B cells and bone marrow cells. The antigen defined by this R-1-3B3 antibody occurred more ab...
Article
The spectrum of differentiation antigens on lymphocytes, defined by monoclonal antibodies and xenoantisera, was considered to determine the stages of lymphocyte ontogeny. Other accompanied surface markers and functions of T and B cells also supported the idea. Tumor cells of lymphoid malignancies can be reasonably identified by their marker profile...
Article
Xenoantisera, designated AT48 and AT72, were developed by immunizing rabbits with human thymus cell membrane and guinea-pigs with a T-cell glycoprotein purified from leukaemic T-cell membrane. Whereas AT48, after appropriate absorption, reacted exclusively with the majority of thymocytes (mainly cortical thymocytes) among normal lymphoid population...
Article
A xenoantiserum was raised in guinea pigs to a human T cell antigen (Tgp72) partially purified from cell membrane of T cell leukemia cells from a patient with chronic lymphosarcoma cell leukemia of T cell-type by using sequential chromatographic procedures. The antiserum reacted with all of human thymus and peripheral T cells as well as with a vari...
Article
Radioimmunoassay, using radiolabeled melanoma-associated antigens (MAA), rabbit anti-B16 melanoma serum and Staphylococcus aureus Cowan I, was developed for the detection of MAA in tumor tissues as well as in sera of B16 melanoma-bearing C57BL/6 mice. MAA were partially purified from 3 M KC1 extract of murine B16 melanoma tissue by affinity column...
Article
Spleen cells from rats bearing syngeneic methylcholanthrene-induced sarcomas showed impaired proliferative responses to phytohemagglutinin (PHA) as well as to the KCl tumor extract. Studies were then performed to define suppressor cell systems possibly operating in tumor-bearing rats. For this purpose, thymus and spleen cells from tumor-bearing rat...
Article
Five cases of gastrointestinal lymphomas were studied in relation to their surface antigenic markers by using specific antisera against human B cells or T cells. Frozen sections of the tumor tissues stained by immunofluorescence with these antisera were utilized for the detection of surface antigens on the lymphoma cells. The lymphomas reported inc...
Article
The nature of cell-dependent antibody (CDA) and the mechanism of inhibition of antibody-dependent cellular cytotoxicity (ADCC) were studied in the ADCC assay system in which culture cells of methylcholanthrene-induced rat fibrosarcoma (KMT-50) were used as target cells, xenogeneic antiserum (rabbit anti-KMT-50) as the CDA, and human peripheral bloo...
Article
A new type of differentiation antigens on human T cells was demonstrated by using a heterologous anti-human T cell serum (ATS). This type of antigen, referred to as human peripheral T cell antigen (HPTA), was found on peripheral T cells and medullary thymocytes, but not on cortical thymocytes and B cells. The percentage of ATS-reactive lymphocytes...
Article
Anti-rat T lymphocyte serum (ATLS)2 was prepared by immunizing rabbits with purified T cells from rat mesenteric nodes and absorbed with rat red cells and syngeneic sarcoma cells. The specificity of ATLS for rat T cells was confirmed by the following reasons: a) ATLS was not toxic for bone marrow cells but lysed most of the thymocytes and a number...
Article
The role of macrophages from rats treated with a streptococcal preparation (OK-432) for inhibition of syngeneic tumor growth was examined both in vivo and in vitro. Treatment of rats with OK-432 intraperitoneally resulted in a transient inhibition of ascites tumor growth and concomitant increase in survival time. Peritoneal exudate cells from these...
Article
In 43 cases of leukemia and malignant lymphoma the presence of surface antigens and receptors for human T and B cells was tested. Rosetting with sheep erythrocytes was used as a T cell marker. Heterologous antisera, which were rendered specific for human thymocytes (ATS-T) or for peripheral T cells (ALS-T), were also employed. In addition, the auth...
Article
Surface antigenic specificities of human thymus-derived (T) lymphocytes were studied by cytotoxicity tests using a heterologous rabbit anti-human thymus serum. This serum showed higher cytotoxic titres on thymocytes by comparison with peripheral lymphocytes. After proper absorption the antiserum was non-toxic for chronic lymphatic leukaemia cells,...
Article
Anti-human B cell serum (ABS) was developed by sequentially absorbing a rabbit anti-human tonsil serum (AHTS) with human red cells, liver, serum, and thymocytes. AHTS was also absorbed quantitatively with tissues and cells from different sources. ABS was nontoxic for thymocytes but lysed the majority of chronic lymphocytic leukemia (CLL) cells. Thi...
Article
Cell mediated tumor specific immune resistance was clearly demonstrated in vivo and in vitro in autochthonous tumor host systems using 3 methylcholanthrene (MCA) induced rat sarcomas. Serum cytotoxicity was only rarely found even with serum from highly resistant hosts. This does not necessarily indicate lack of participation of humoral antibodies;...
Article
Summary The local GVH reaction was produced by grafting lymphoid cells from parental strain rats under the kidney capsule of F 1 hybrid recipients. The ultrastructural aspects of cells proliferating in the reaction site of the kidney allowed classification into two categories, lymphocytic and plasmacytic. The lymphocytic cells showed a range of ult...

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