Yukio Yoshioka

Hiroshima University | HU · Department of Applied Molecular and Cellular Biology

The immune checkpoint inhibitor (ICI), nivolumab, has revolutionised the treatment of recurrent and metastatic oral cancer. However, the response rate to ICIs remains low, and identifying predictors of nivolumab response is critical. Although the neutrophil-to-lymphocyte ratio (NLR) has been suggested as a predictive marker of nivolumab response in...

Vascular endothelial growth factor A (VEGF-A) and its receptors (VEGFR1 and R2) play important roles in the progression of malignant melanoma through tumor angiogenesis. However, it is not clear whether the VEGF-A/VEGFR1 signaling pathway is involved in the proliferation and migration of melanoma cells. Thus, the effect of VEGF-A on cell migration...

Malocclusion and morphological abnormalities of the jawbone often affect the stomatognathic function and long-term postoperative stability in patients with jaw deformities. There are few reports on the effect of maximum tongue pressure (MTP) in these patients. We investigated the relationship between the MTP and jawbone morphology and the effect of...

Herein, we report on a 66‐year‐old woman with locoregional advanced tongue cancer. She was diagnosed with left side tongue carcinoma and achieved complete remission using nivolumab monotherapy after induction chemotherapy (IC) with docetaxel, cisplatin, and 5‐fluorouracil, followed by intensity‐modulated bioradiotherapy and cetuximab monotherapy. F...

Cancer metastasis and recurrence are potentially lethal. A small number of cancer cell groups called cancer stem cells (CSCs) have both stem cell capacity and cancer-forming ability and are reported to play important roles in cancer metastasis and recurrence. These CSCs are considered to be radiation-resistant (RR). Therefore, understanding the bio...

Purpose To determine the clinical and therapeutic characteristics in adolescent and young adult (AYA) generation with oral cancer. Methods We retrospectively studied AYA patients with oral cancer, aged 16‐40 years who visited our department between 1 April 1997 and 31 December 2017, focusing on clinical characteristics including sex, age distribut...

Restoring the cosmetic and functional aspects of the lip after tumor resection is challenging. We report a case of reconstruction for a defect due to resection of a melanoma using a lower lip musculomucosal flap combined with a tongue flap. A 20-year-old man was referred to our hospital and diagnosed with malignant melanoma with metastatic right su...

Carcinoma ex pleomorphic adenoma is a rare type of cancer of the salivary gland that involves the malignant transformation of a primary or recurrent pleomorphic adenoma, which often metastasises to the lungs or bones, or both. To the best of our knowledge, however, nobody has reported a distant metastasis of this lesion to the brain without such pr...

The control of local failure (LF) is essential to improve outcomes in patients with squamous cell carcinoma of the oral cavity (OSCC). In this study, LF of OSCC was classified into three clinical types: deep recurrence (type 1R), adjacent superficial recurrence (type 2R), and distant primary tumour (type 3R). LF was analyzed after surgical resectio...

Introduction: Suture granuloma is a benign tumor that develops because of the presence of surgical suture materials. It commonly occurs several years after different types of surgeries. Here we report a case involving a 64-year-old man who underwent head and neck surgery for oral squamous cell carcinoma and developed multiple suture granulomas mim...

A crude methanolic extract of the Indonesian sponge Clathria bulbotoxa showed a potent cytotoxic activity against the human epidermoid carcinoma A431 cells. An investigation of the active components led to the isolation of three new compounds named crambescidins 345 (1), 361 (2), and 373 (3), together with the known related metabolites crambescidin...

In the background of the abnormal intraoral hematoma formation, there exists a possible underlying severe bleeding disorders. In this report, we described a case of fibrinolytic activated disseminated intravascular coagulation（DIC）associated with an abdominal aortic aneurysm, and that of idiopathic thrombocytopenic purpura（ITP）revealed by intraoral...

Objectives: Photodynamic therapy (PDT) is a very effective treatment for superficial malignancies that does not result in loss of normal tissue. Here, we report successful PDT treatment of superficial oral cancers and its clinical outcome with long-term follow up. Materials and methods: Thirty-four superficial oral squamous cell carcinomas were...

Adenoid cystic carcinoma (ACC) of the salivary gland often metastasizes to the lungs. It has previously been reported that the combination chemotherapy of weekly paclitaxel plus cetuximab is effective for the treatment of lung metastasis from ACC of the salivary gland. Here, we report a case in which a regimen of this combination therapy provided a...

Bisphosphonates (BPs) have been used in medical practice for the treatment of osteoporosis, bone metastasis, and multiple myeloma. Although many studies have been published, the treatment and prognosis of bisphosphonate-related osteonecrosis of the jaw (BRONJ) remain unclear. This study included 59 patients with BRONJ: 29 had taken oral BPs and 30...

Bisphosphonates (BP) are potent inhibitors of the osteoclast activity used in the treatment of metastatic bone disease and osteoporosis. Bisphosphonate related osteonecrosis of the jaw (BRONJ) is a rare, but severe complication as a pathological fracture, pain and tumor-induced hypercalcemia. Patients taking BP may subsequently develop BRONJ after...

The objective of this study was to investigate the histogenesis of ectomesenchymal chondromyxoid tumors (ECTs) of the tongue. The biochemical characteristics of a rarely occurring tumor of the tongue were analyzed by immunohistochemistry and reverse-transcriptase polymerase chain reaction (RT-PCR), and its biological properties were assessed in pri...

From an extract of the Okinawan sponge Petrosia strongylata, five novel acetylenic metabolites, petrosiols A (1)–E (5), were isolated and chemically characterized. These compounds induced nerve growth factor (NGF)-like neuronal differentiation of PC12 cells. The structure–activity relationships of the petrosiols, their derivatives, and some related...

Ameloblastic carcinoma is a rare, odontogenic, malignant tumor that has features of ameloblastoma in addition to cytologic atypia with or without metastasis. It is classified as primary type; secondary type, intraosseous; and secondary type, peripheral according to the World Health Organization classification of 2005. Ameloblastic carcinoma, second...

A library of extracts established from hundreds of marine organisms was screened by a cytotoxicity test. The active organic extract of an Okinawan marine sponge of the genus Dysidea was subjected to bioassay-guided fractionation to give three new polyoxygenated steroids dysideasterols F-H (1-3), together with two known related compounds (4 and 5)....

A new 10-membered-ring diterpene, cyclolobatriene (1), along with three other known diterpenes, lobatriene (2), eunicol (3), and fuscol (4), were isolated from the Okinawan soft coral Lobophytum pauciflorum. Their structures were established by extensive NMR spectroscopic analyses. Cyclolobatriene (1) is an additional example of rare prenylated ger...

Novel nitrogenous diterpenoids, spongolactams A-C (1-3), were isolated as trace components of an Okinawan marine sponge, Spongia sp., by an LC/MS-guided assay for farnesyl transferase (FTase) inhibitors. Their structures were elucidated by spectroscopic analyses. To evaluate their structures and biological activity, the metabolites were semisynthes...

A long-chain polyhydroxy polyene amide, zooxanthellamide D (ZAD-D, 1, C54H83NO19), was isolated from a cultured marine dinoflagellate of the genus Symbiodinium. ZAD-D (1) is a polyhydroxy amide consisting of a C22-acid part and a C32-amine part and furnishes three tetrahydropyran rings and six isolated butadiene chromophores. The relative stereoche...

Primary intraosseous squamous cell carcinoma (SCC) is a rare malignant tumor of the jaw. We report a case of intraosseous SCC involving the mandible of a 60-year-old male. The serum level of carcinoembryonic antigen (CEA) was exceedingly elevated. Immunohistochemical analysis showed that the tumor was strongly stained with anti-CEA antibody. The pa...

Xestoquinone and related metabolites (the xestoquinone family) occur in marine sponges and are known to show a variety of biological activities. In this study, the first comprehensive evaluation of antifungal activity was performed for xestoquinone and nine natural and unnatural analogues in comparison with their cytotoxicity. The cytotoxicity agai...

The participation of plasminogen activator/plasmin system in the expression and function of E-cadherin was examined in oral squamous cell carcinoma (SCC) cells. Treatment of SCC cells with plasminogen reduced the Ca2+-dependent cell aggregation. SCC cells expressed E-cadherin at the cell membrane, and released a small amount of soluble E-cadherin a...

Leiomyosarcoma of the oral cavity is a rare malignant mesenchymal tumour: This report is of a 60-year-old woman with a leiomyosarcoma involving the mandible. Histologically, the tumour was composed of Spindleshaped cells with oval nuclei and eosinophilic cytoplasm, and atypical mitotic figures were detected. Immunoreactivity for desmin, vimentin, a...

We report a case of dedifferentiated adenoid cystic carcinoma (ACC) of the submaxillary gland. A 70 year-old female was referred with rapidly growing tumor at the left cervical region. Tumor resection associated with radical neck dissection was performed. Subsequently, the patient underwent chemoradiation therapy using cisplatin (CDDP) and 5-fluoro...

A novel fatty acid derivative named zooxanthellactone (ZL) was isolated from several strains of symbiotic microalgae, dinoflagellates of the genus Symbiodinium. The metabolite is structurally related to docosahexaenoic acid (DHA) and seems to be biosynthesized by oxidation and subsequent lactonization. The absolute stereochemistry was determined fr...

Metastatic tumors of the oral region are rare, representing about 1 % of malignant tumors in the oral cavity. In this article, we report a case of carcinoma of the sigmoid colon that metastasized to the maxillary gingiva and review of literature. A 69-year-old man underwent extraction of the right first and second molars of the maxilla, and a rapid...

The immunohistochemical localization of thrombospondin-1 (TSP-1) in human normal oral mucosa and oral squamous cell carcinoma (SCC) was examined. The immunostaining of TSP-1 was mainly localized in the connective tissues adjacent to the epithelium of oral mucosa, whereas epithelial cells showed negligible immunoreactivity for TSP-1. TSP-1 expressio...

The effect of fibroblasts on the activation of matrix metalloproteinase-2 (MMP-2) in oral squamous cell carcinoma (SCC) cells was examined. The plasma membrane of SCC cells failed to bind and activate latent MMP-2. However, treatment of SCC cells with fibroblast-conditioned medium (fibroblast-CM) led to the enhancement of the binding and activation...

The proliferation of mouse submandibular gland carcinoma YT-12 cells was stimulated by endothelial cell growth factor (ECGF)/bovine brain-derived acidic fibroblast growth factor (aFGF) and recombinant human aFGF. To determine whether aFGF was capable of modifying salivary gland carcinogenesis, the effect of brain-derived aFGF was examined in vivo....

This study investigated whether epidermal growth factor (EGF) administration was capable of modifying salivary gland carcinogenesis. Two groups of mice were given 1 mg of 9, 10-dimethyl-1,2-benzanthracene (DMBA) into the left submandibular gland, and then Group 1 mice received 2 μg of EGF and Group 2 mice received vehicle subcutaneously for 8 weeks...

The effect of protein tyrosine kinase (PTK) inhibitors on the replication of herpes simplex virus (HSV) was examined. Tyrphostins AG17, AG213, AG490, and AG555, and herbimycin A all inhibited the plaque formation of HSV type 1 (HSV-1) in Vero cells, but AG17, AG490, and AG555 exhibited a more selective antiviral effect. In the presence of 0.4 micro...

A case of osseous choristoma in the buccal mucosa of a 9 -year-old girl is described. In the literature only 11 cases have been reported previously. The lesion measured 8.5×8.5mm and was removed under local anesthesia.Histologically, the lesion consisted of a well circumscribed mass of dense lamellar bone, surrouded by a thin layer of fibrous conne...