Yuan Pang

Yuan Pang
Tsinghua University | TH · Department of Mechanical Engineering

PhD

About

41
Publications
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353
Citations

Publications

Publications (41)
Article
Nanocatalytic medicine triggering in situ catalytic reactions has been considered as a promising strategy for tumor-selective therapeutics. However, the targeted distribution of nanocatalysts was still low, considering the absence of targeting propulsion capability. Here, encouraged by the fast-developing controllable microrobotics for targeting de...
Article
Anti‐Tumor Drug Screening In article 2200863 by Huayu Yang, Yuan Pang, and co‐workers, a polydimethylsiloxane (PDMS)‐based oxygen‐permeable microwell device is introduced for generating highly viable and functional hepatocellular carcinoma (HCC) spheroids. The PDMS sheets of the microwell device provide high flux oxygen and function like in vivo ne...
Article
Full-text available
Hepatic alveolar echinococcosis (HAE) is a zoonotic parasitic disease caused by the larvae of Echinococcus multilocularis . Because of its characteristics of diffuse infiltration and growth similar to tumors, the disability rate and mortality rate are high among patients. Although surgery (including hepatectomy, liver transplantation, and autologou...
Article
Bone repair is a complex, multi-stage process, involving angiogenesis and osteogenesis, under the control of sequential stimulation from multiple factors such as FGFs, VEGF, and BMP2. Promoting the bone repair processes at different stages can greatly shorten the period and improve the quality of repair. How to deliver angiogenic and osteogenic cue...
Article
For high‐throughput anti‐cancer drug screening, microwell arrays could serve as an effective tool to generate uniform and scalable tumor spheroids. However, microwell arrays are commonly anchored in non‐oxygen‐permeable culture plates, leading to limited oxygen supply for avascular spheroids. Herein, a PDMS‐based oxygen‐permeable microwell device w...
Article
Increasing lung diseases, mutating coronaviruses, and the development of new compounds urgently require biomimetic in vitro lung models for lung pathology, toxicology, and pharmacology. The current construction strategies for lung models mainly include animal models, 2D cell culture, lung-on-a-chip, and lung organoids. However, current models face...
Article
Full-text available
Cervical cancer induced by human papillomavirus (HPV) causes severe morbidity worldwide. Although cervical conization has been widely accepted as the most conventional surgery against cervical cancer, tissue defects and high recurrence rates have a significant negative impact on women's mental and physical health. Herein we developed an implantable...
Article
Full-text available
Hydrogels have recently received attention as delivery carriers owing to their good biocompatibility and structural similarity to natural extracellular matrices. However, the utilization of traditional single-network (SN) hydrogels is limited by poor mechanical properties and burst drug release. Therefore, we developed a novel double-network (DN) h...
Article
The cover image is based on the Communication to the Editor Ferric ion crosslinking‐based 3D printing of a graphene oxide hydrogel and its evaluation as a bio‐scaff old in tissue engineering by Renhao Lu et al., https://doi.org/10.1002/bit.27592.
Article
Full-text available
Bioelectrochemical systems (BESs) can fulfill the demand for renewable energy and wastewater treatment, but still face significant challenges to improve their overall performance. Core efforts have been made to enhance the bioelectrode performance, yet, previous approaches are fragmented and have limited applicability, unable to flexibly adjust phy...
Article
To address the remaining issue of poor cell immobilization and insufficient mass transfer in scaffold-based tissue engineering approach for future islet transplantation, we employed a macro-porous poly-l-lactide (PLLA) scaffold immobilizing mouse insulinoma cells and studied its function toward an implantable pancreatic tissue in 7-day perfusion cu...
Article
Background: In liver tissue engineering, co-culturing hepatocytes with typical non-parenchymal hepatic cells to form cell aggregates is available to mimic the in vivo microenvironment and promote cell biological functions. With a modular assembly approach, endothelialized hepatic cell aggregates can be packed for perfusion culture, which enables t...
Article
As a precursor of graphene, graphene oxide (GO) exhibits excellent mechanical, thermal, and electrical properties, besides appreciable biocompatibility in tissue engineering applications. However, the current GO‐3D fabrication technology is still in need of optimization and simplification to ensure fine architecture and reasonable mechanical proper...
Article
Hepatocellular carcinoma (HCC) is one of the most lethal tumors worldwide. This study aims to address the lack of faithful and available in vitro models for patient-specific drug screening for HCC. We recently established a novel modeling system using three-dimensional (3D) bioprinting technology and constructed hepatorganoids with HepaRG cells, wh...
Article
Full-text available
Cervical cancer induced by human papillomavirus (HPV) causes severe morbidity worldwide. Although cervical conization has been widely accepted as the most conventional surgery against cervical cancer, tissue defects and high recurrence rates have a significant negative impact on women's mental and physical health. Herein we developed an implantable...
Article
Towards the development of in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy, patient-derived primary tumor cells were employed in this work for three-dimensional (3D) bioprinting. Intrahepatic cholangiocarcinoma cells isolated from patient were bioprinted using a composite hydrogel system...
Article
Full-text available
The existing in vitro models for antitumor drug screening have great limitations. Many compounds that inhibit 2D cultured cells do not exhibit the same pharmacological effects in vivo, thereby wasting human and material resources as well as time during drug development. Therefore, developing new models is critical. The 3D bioprinting technology has...
Article
Studying biological characteristics of tumors and evaluating the treatment effects require appropriate in vitro tumor models. However, the occurrence, progression, and migration of tumors involve spatiotemporal changes, cell-microenvironment and cell-cell interactions, and signal transmission in cells, which makes the construction of in vitro tumor...
Article
Full-text available
The aim of the present study was to design and fabricate polyglycolic acid (PGA) modules on the basis of the Raschig ring as a tissue element for bottom–top tissue engineering to increase the feasibility of cellular-assembly technology. Three types of modules, namely, cylindrical, Raschig ring, and transverse-pore modules, with different numbers an...
Article
Full-text available
Objective Shortage of organ donors, a critical challenge for treatment of end-stage organ failure, has motivated the development of alternative strategies to generate organs in vitro. Here, we aim to describe the hepatorganoids, which is a liver tissue model generated by three-dimensional (3D) bioprinting of HepaRG cells and investigate its liver f...
Article
In order to address the remaining issues of fragile structure and insufficient mass transfer faced in modular assembly-based liver tissue engineering, a Raschig ring-like hollowed micro-scaffold was proposed and fabricated using poly-ε-caprolactone with 60% porosity and 11.4 mm2 effective surface area for cell immobilization. The method of cell ino...
Article
Resolving the origin and development of tumor heterogeneity has proven to be a crucial challenge in cancer research. In vitro tumor models have been widely used for both scientific and clinical research. Currently, tumor models based on 2D cell culture, animal models and 3D cell‐laden constructs are widely used. Heterogeneous tumor models, which co...
Article
Constructing in vitro drug models with a higher degree of bionics is considered to be an important approach to improve the effectiveness of existing drug screening models. 3D printing, co-culture and microfluidics are important methods that commonly used. This study combined the advantages of the three methods. A 3D co-culture microfluidic model wi...
Article
A new three-dimensional (3D) cell printing system was developed and investigated to organize multiple cells/biomaterials with a control precision within 100 lm. This system can be used for the in vitro con- struction of heterogeneous tissue models. The proposed printing system was achieved by the integration of extrusion printing and alternating vi...
Article
A novel biodegradable micro-scaffold with intersecting hollow channels was designed as tissue elements to address the main challenges in current modular assembly-based tissue engineering-the lack of rigid structures to sustain in vivo-like flow conditions, and delicate design to facilitate mass transfer to the cultured cells towards scaling upto hi...
Article
Advanced in vitro cervical tumor model was established by 3D printing to study the epithelial-to-mesenchymal transition (EMT), which is a very important stage of dissemination of carcinoma leading to metastatic tumors. A Hela/hydrogel grid construct composed of gelatin, alginate, matrigel and Hela cells was fabricated by forced extrusion in a layer...
Article
Full-text available
A capillary electrophoresis (CE) incorporating chemiluminescence (CL) detection method was applied to on-line screening and evaluation of antioxidants in alkaloid natural products. Constant and stable CL intensity was derived from the reaction of luminol with reactive oxygen species (ROS) which were generated from a sodium hypochlorite-hydrogen per...
Article
A novel engineering methodology for organizing a large liver tissue equivalent was established by intergrating both 'top down' and 'bottom up' approaches. A three-dimensional (3D) scaffold was engineered comprising 43 culture chambers (volume: 11.63 cm(3)) assembled in a symmetrical pattern on 3 layers, a design which enables further scaling up of...
Article
To realize long-term in vitro culture of hepatocytes at a high density while maintaining a high hepatic function for aggregate-based liver tissue engineering, we report here a novel culture method whereby endothelialized rat hepatocyte aggregates were formed using a PDMS microwell device and cultured in a perfusion bioreactor by introducing spacers...
Article
The antioxidantive biological fingerprints of astragalus was established by capillary electrophoresis (CE) with chemiluminescence (CL) detection. 6 co-possessing peaks were selected as the fingerprints of astragalus and astragaloside was selected as the reference peak. The ability to scavenge radical of astragaloside was confirmed for the first tim...

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