Yuan-Chii G Lee

Yuan-Chii G Lee
Taipei Medical University | TMU · Graduate Institute of Biomedical Informatics

PhD

About

38
Publications
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1,229
Citations

Publications

Publications (38)
Article
Apoptotic chromatin condensation inducer in the nucleus (also referred as Acin1) was first characterized as an RNA-binding protein involved in apoptosis. In later reports, Acin1 was identified as an auxiliary component of the exon junction complex (EJC) which is assembled throughout pre-messenger RNA splicing. In this study, results of whole-transc...
Article
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The therapeutic effects of simvastatin for renal cell carcinoma (RCC) are controversial. In this study, the effects of simvastatin on the carcinogenic properties of 3-methylcholanthrene (3MC; an aryl-hydrocarbon receptor [AhR] agonist) in human renal epithelial cells (hRECs) were investigated. We exposed in vitro and in vivo models to 3MC to induce...
Article
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Induced brown adipocytes (also referred to as beige cells) execute thermogenesis, as do the classical adipocytes by consuming stored lipids, being related to metabolic homeostasis. Treatment of phytochemicals, including berberine (BBR), was reported to induce conversion from white adipocytes to beige cells. In this study, results of microRNA (miRNA...
Article
Background We previously showed that 3-O‐β‐D‐glucopyranosyl‐(3R)‐hydroxybutanolide (kinsenoside), a major compound of Anoectochilus formosanus, increased lipolysis through an AMP–activated protein kinase (AMPK)–dependent pathway. Purpose To extend our previous finding, we investigated the in vivo and in vitro effects of kinsenoside on lipolysis an...
Article
Alternative splicing (AS) of pre-messenger (m)RNA is a pivotal mechanism in expanding proteomic diversity, which determines the functions of mammalian cells. By conducting transcriptome analyses to profile splicing events in human colorectal cancer (CRC) tissues compared to adjacent normal counterparts, we noted differential splicing profiles of se...
Article
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A growing body of studies has demonstrated that dysregulated splicing profiles constitute pivotal mechanisms for carcinogenesis. In this study, we identified discriminative splicing profiles of colorectal cancer (CRC) cells compared to adjacent normal tissues using deep RNA-sequencing (RNA-seq). The RNA-seq results and cohort studies indicated a re...
Article
Endothelial nitric oxide synthase (eNOS) modulates vascular blood pressure and is predominantly expressed in endothelial cells and activated through the protein kinase B (Akt/PKB)-dependent pathway. We previously reported that 3-methylcholanthrene (3MC) activates the aryl hydrocarbon receptor (AhR) and reduces PI3K/Akt phosphorylation. This study i...
Article
Keloids is a fibroproliferative disease. The incidence of keloids among Asians has not been thoroughly studied. The objective of this study is to determine the incidence of keloids in Taiwan, which mainly consists of ethnic Chinese. Furthermore, we want to determine the comorbidity rate of other fibrosis-related diseases among keloid patients. This...
Article
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Objective: Toll-like receptors (TLRs) are important molecules for detecting both pathogen invasion and tissue damage. The expression of TLR4 is upregulated in ischemic stroke, at least in the subacute stage. However, the TLR downstream pathways in the context of stroke have not been well studied in previous research. The purpose of this study is t...
Article
Increasing evidence suggests that PRMT5, a protein arginine methyltransferase, is involved in tumorigenesis. However, no systematic research has demonstrated the cell-transforming activity of PRMT5. We investigated the involvement of PRMT5 in tumor formation. First, we showed that PRMT5 was associated with many human cancers, through statistical an...
Article
In an attempt to search for genes with abnormal expression in cancers, Suppressed in Lung Cancer (SLAN, also known as KIAA0256) is found underexpressed in human lung cancer tissues by quantitative real-time PCR (Q-RT-PCR). The study set out to characterize SLAN protein and explore its cellular functions. SLAN or its specific short hairpin RNA, full...
Article
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A cross-talk between different receptor tyrosine kinases (RTKs) plays an important role in the pathogenesis of human cancers. Both NIH-Met5 and T24-Met3 cell lines harboring an inducible human c-Met gene were established. C-Met-related RTKs were screened by RTK microarray analysis. The cross-talk of RTKs was demonstrated by Western blotting and con...
Data
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c-Met expression in NIH/3T3 and NIH-Met5 cell lines in the presence or absence of Tet and/or HGF. The cells and the treatment is the same as Figure 1A, except the expression of IgG was shown as the loading control. M: protein marker.
Data
The relationship among c-Met, Sp1, Axl and PDGFR-α demonstrated by c-Met siRNA. NIH-Met5 cells (1 × 106/plate) were transfected with c-Met siRNA (250 nM and 500 nM) for 24 h. Then, cells were harvested and total protein was extracted and analyzed for c-Met, Sp1, Axl and PDGFR-α expression by Western blotting. β-actin was used as the internal contro...
Article
The estrogen receptor alpha gene (ESR1) is an important mediator of the atheroprotective effect of estrogen on the vasculature system. We examined the potential associations between common single nucleotide polymorphism (SNP) variants of ESR1 and intima-media thickness (IMT) in carotid arteries, a strong predictor of cardiovascular disease (CVD). A...
Data
Oncology. FLJ10540 mediates cell migration and invasion through the PI3K/AKT signaling pathway. (A). The migration and invasion photography results of vehicle-CL1-0, CL1-0-FLJ10540 stable clones, vehicle-RK3E and RK3E-FLJ10540 infected cells were treated with or without LY294002 at the final concentration of 10 mM are shown (200×). The data represe...
Data
Oncology. FLJ10540 overexpression enhances motility of RK3E cells. (A) FLJ10540 stable clones of retrovirus infected Flag-tagged FLJ10540 in RK3E cells was established. The cell lysates were subjected to immunoblot analysis with anti-Flag antibody. (B) For the migration assay, 5×103 cells of vehicle-RK3E and RK3E-FLJ10540 infected cells were seeded...
Data
Oncology. Molecular portraits of lung adenocarcinoma. (A) Supervised hierarchical clustering showed the 826 (634 down- and 192 up-regulated) transcript expression patterns of 26-pairwise lung adenocarcinoma samples. The results were shown in a dendrogram format, in which rows represent individual transcripts and columns represent tissue samples. Es...
Article
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Background: Lung adenocarcinoma is the leading cause of cancer-related deaths among both men and women in the world. Despite recent advances in diagnosis and treatment, the mortality rates with an overall 5-year survival of only 15%. This high mortality is probably attributable to early metastasis. Although several well-known markers correlated wi...
Conference Paper
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The continuous quality improvement has become a trend in the contemporary medical society, and that can be achieved by the specialty database implement. Decision-support system in the academic and clinical aspects are included in the process such continuous quality improvement. The database has its limitation in the decision-support due to deficien...
Article
A significant challenge in the post-genomic era is how to prioritize differentially expressed and uncharacterized novel genes found in hepatocellular carcinoma (HCC) microarray profiling. One such category is cell cycle regulated genes that have only evolved in higher organisms but not in lower eukaryotic cells. Characterization of these genes may...
Article
The explosion of microarray studies has promised to shed light on the identification of disease markers. To find novel prognostic factors, we used the expression profile of a poor prognostic factor of lung cancer, survivin (BIRC5), as a template to search for and compare transcriptome expression profiles in a lung adenocarcinoma microarray dataset....
Article
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It was proposed that Epstein-Barr virus (EBV) is closely associated with nasopharyngeal carcinoma (NPC); however, the molecular mechanisms involved in the effect of EBV on NPC host genes have not yet been well defined. For this study, two sets of microarray experiments, NPC (EBV-free) vs normal epithelial cells and EBV(+) vs EBV(-) NPC arrays, were...
Article
Benzo[a]pyrene (BaP) is one of many polycyclic aromatic hydrocarbons that have been identified as major risk factors for developing various cancers. We previously demonstrated that the liver cancer susceptibility gene glycine N-methyltransferase (GNMT) is capable of binding with BaP and protecting cells from BaP-7,8-diol 9,10-epoxide-DNA adduct for...
Article
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High-throughput techniques such as cDNA microarray, oligonucleotide arrays, and serial analysis of gene expression (SAGE) have been developed and used to automatically screen huge amounts of gene expression data. However, researchers usually spend lots of time and money on discovering gene-disease relationships by utilizing these techniques. We pro...
Article
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Aurora-A, a mitotic serine/threonine kinase with oncogene characteristics, has recently drawn intense attention because of its association with the development of human cancers and its relationship with mitotic progression. Using the gene expression profiles of Aurora-A as a template to search for and compare transcriptome expression profiles in pu...
Article
We examined the metal ion cofactor preference for MST3 (mammalian Ste20-like kinase 3) of the Ste20 serine/threonine kinase family. Four metal ions (Mg(+2), Mn(+2), Zn(2+), and Co(2+)) activate endogenous, exogenous, and baculovirus-expressed recombinant MST3 within the physiological concentration range. In contrast, Fe(+2) and Ca(+2) do not functi...
Article
Full-text available
One possible path towards understanding the biological function of a target protein is through the discovery of how it interfaces within protein–protein interaction networks. The goal of this study was to create a virtual protein–protein interaction model using the concepts of orthologous conservation (or interologs) to elucidate the interacting ne...
Article
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F-box proteins, components of SCF ubiquitin-ligase complexes, are believed to be responsible for substrate recognition and recruitment in SCF-mediated proteolysis. F-box proteins that have been identified to function in the SCF complexes to date mostly have substrate-binding motifs, such as WD repeats or leucine-rich repeats in their C termini. How...
Article
Full-text available
The increasing use of high-throughput and large-scale bioinformatics-based studies has generated a massive amount of data stored in a number of different databases. The major need now is to explore this disparate data to find biologically relevant interactions and pathways. Thus, in the post-genomic era, there is clearly a need for the development...
Article
Numerous genetic changes are associated with metastasis of cancer cells. Previously, we used microarray to identify that collapsin response mediator protein-1 (CRMP-1) was involved in cancer invasion and metastasis. We further characterized that CRMP-1 was a novel invasion-suppression gene. Members of the CRMP gene family are intracellular phosphop...
Article
Full-text available
An analytic strategy was followed to identify putative regulatory genes during the development of human hepatocellular carcinoma (HCC). This strategy employed a bioinformatics analysis that used a database search to identify genes, which are differentially expressed in human HCC and are also under cell cycle regulation. A novel cell cycle regulated...

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