Yu-Jui Fan

Yu-Jui Fan
Taipei Medical University | TMU · College of Biomedical Engineering

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35
Publications
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282
Citations

Publications

Publications (35)
Article
C-Reactive protein (CRP) is an essential biomarker relevant to various disease prognoses. Current biosensors require a significant amount of time for detecting CRP. To address this issue, this work proposes electrokinetic flow-assisted molecule trapping integrated with an impedance biosensor, where a driving signal in terms of a gated sine wave is...
Article
Full-text available
In this study, microneedle-integrated light sheet microscopy (LSM) was developed for trapping and continuously imaging embryos of Caenorhabditis elegans with subcellular resolution. To reduce aberrations when the light sheet was propagated into the device, a microneedle was fabricated using a transparent, water refractive index-matched polymer. It...
Article
Noninvasive photothermal therapy (PTT) represents a promising direction for more modern and precise medical applications. However, PTT efficacy is still not satisfactory due to the existence of heat shock proteins (HSPs) and poorly targeted delivery. Herein, the design of a nanosystem with improved delivery efficacy for anticancer treatment employi...
Article
Epstein-Barr virus (EBV) positivity is one of the indexes for diagnosing nasopharyngeal carcinoma (NPC). Moreover, systemic inflammatory responses can easily be triggered in patients who are both EBV- and coronavirus disease 2019 (COVID-19)-positive. Development of rapid and highly sensitive EBV screening methods has become important. In this study...
Article
In this study, we developed a portable solar-powered centrifuge designed to provide access to centrifugation in remote areas of the world with scarce resources. The autoantibodies in patients’ blood, after centrifuging, were evaluated by nanoplamonic chip integrated with smartphone reader for rheumatoid arthritis detection. The centrifuge is capabl...
Article
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In this study, a multiplex detection system was proposed by integrating a localized surface plasmon resonance (LSPR) sensing array and parallel microfluidic channels. The LSPR sensing array was fabricated by nanoimprinting and gold sputter on a polycarbonate (PC) substrate. The polydimethylsiloxane (PDMS) microfluidic channels and PC LSPR sensing a...
Article
We present the first combination of a microfluidic polymerase chain reaction (PCR) with a gold nanoslit-based surface plasmon resonance (SPR) sensor for detecting the DNA sequence of latent membrane protein 1 (LMP1). The PCR microchannel was produced through a laser scribing technique, and the SPR nanoslit chip was manufactured via hot-embossing na...
Article
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Natural porous scaffolds have been studied and developed for decades in biomedical science in order to support cells with a simulated extracellular matrix in natural tissue as an ideal environment. Such three-dimensional scaffolds provide many degrees of freedom to modulate cell activity, such as porosity, pore size, mechanical strength, biodegrada...
Article
Near-infrared (NIR)-light-modulated photothermal thrombolysis has been investigated to overcome the hemorrhage danger posed by clinical clot-busting substances. A long-standing issue in thrombosis fibrinolytics is the lack of lesion-specific therapy, which should not be ignored. Herein, a novel thrombolysis therapy using photothermal disintegration...
Article
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Candida albicans is a commensal fungus that is responsible for a lot of nosocomial infections in immunocompromised people. Cell culture is currently the predominant method for diagnosing candidiasis, but it is time consuming. In this study, we developed a rapid screen procedure by devising a method for detecting C. albicans with the use of electroc...
Article
Full-text available
In this study, a continuous cell-imaging system with subcellular resolution was developed by integrating a microfluidic platform with lattice lightsheet microscopy (LLSM). To reduce aberrations of the lightsheet propagating into the device, a microfluidic channel sealed with a water refractive index-matched thin film was fabricated. When the lights...
Article
Microflow cytometry was previously pursued to achieve high-throughput cell analyses. It has advantages of parallelization and miniaturization capabilities that allow fewer detecting elements to simultaneously observe multiple channels. However, the major disadvantage is that it is difficult to precisely control parallel cell positions in each chann...
Article
This paper reports a facile electrochemical detection method for Escherichia coli (E. coli) that does not use DNA amplification or immunoassay. The detection principle is based on the activity of the β-galactosidase (β-gal) endogenous enzyme, which hydrolyzes p-aminophenyl-β-d-galactopyranoside (p-APG) into p-aminophenol. After E. coli consumes p-A...
Article
Contact guidance has been extensively explored using patterned adhesion functionalities that predominantly mimic cell-matrix interactions. Whether similar contact guidance can be driven by other types of interactions, such as cell-cell adhesion, still remains a question. Herein, we address this query by engineering a set of microstrip patterns of (...
Article
Full-text available
We developed a new format for liquid crystal (LC)-based multi-microfluidic immunoassays, hosted on a polydimethylsiloxane substrate. In this design, the orientations of the LCs were strongly affected by the interface between the four microchannel walls and surrounding LCs. When the alignment layer was coated inside a microchannel, the LCs oriented...
Article
Surface plasmon resonance (SPR) nanowire array chips with a microfluidic system are an effective detection method for a rapid test device. This study investigated a capped gold nanowire array and a microfluidic test platform to provide a fundamental understanding of the kinetic binding of SPR nanowires and the surface gold refractive index. The dev...
Article
Preconcentration of biomolecules for detection on microfluidic platforms based on electrical kinetic trapping (EKT) through ion concentration polarization (ICP) has been well developed in the past decade. Biomolecules can be entrapped due to the equilibrium of forces between electro-osmosis and ICP when applying a voltage to the system. However, th...
Article
Full-text available
In this study, we developed a portable smartphone-based diffusometry for analyzing the C-reactive protein (CRP) concentration. An optimized fluorescence microscopic add-on system for a smartphone was used to image the 300 nm fluorescent beads. Sequential nanobead images were recorded for a period and the image data were used for fluorescence correl...
Article
This paper reports prototype atmospheric-pressure plasma jet (APPJ)-processed reduced graphene oxide (rGO)-modified carbon electrochemical sensors integrated with 3D-printed microfluidic channels. Dopamine (DA) solutions with various concentrations are used for the model test. The APPJ-calcined rGO coating significantly enhances the electrochemical...
Article
This paper describes a new sensing technique for detecting orchid viruses by measuring the Brownian diffusion of immunobeads in liquid samples. Both the capsid proteins and virus particles of Odontoglossum ringspot virus (ORSV) are detectable targets by using this technique. When the targets bind to immunobeads, the Brownian diffusion of the beads...
Article
Full-text available
We report a novel methodology for fabricating large-area, multilayer, thin-film, high aspect ratio, 3D microfluidic structures with through-layer vias and open channels that can be bonded between hard substrates. It is realized by utilizing a hybrid stamp with a thin plastic sheet embedded underneath a PDMS surface. This hybrid stamp solves an impo...
Article
This paper reports the in situ detection of C-reactive protein (CRP) and antibody-coated nanobead interaction by measuring the variation of the Brownian diffusion of the nanobeads. The measurements are carried out by using total internal reflective fluorescence-enhanced (TIRF-enhanced) micro-particle-tracking velocimetry (micro-PTV), which is highl...
Article
Full-text available
An electrokinetic trapping (EKT)-based nanofluidic preconcentration device with the capability of label-free monitoring of trapped biomolecules through real-time dual-loop electric current measurement was demonstrated. Universal current-voltage (I-V) curves of EKT-based preconcentration devices, consisting of two microchannels connected by ion-sele...
Conference Paper
Full-text available
We report on a novel optoelectronic tweezers (OET) platform integrated with three-dimensional microfluidic networks to enable single-cell manipulation and advance fluid control functions such as mixing droplets and thermal cycles on a chip.
Conference Paper
Full-text available
In this study, a two-section clinical sample detection on viscosity and bimolecule sensing technique based on measuring the Brownian velocity of nanobeads has been successfully developed. Bare and antibody-coated fluorescent nanobeads were used to detect various viscous solutions and different concentrations of bio-molecular samples, respectively....
Article
A new technique is developed to measure the nanoparticles' brownian motions by employing microparticle-tracking velocimetry (micro-PTV) in evanescent wave field, which can provide high signal-to-noise ratio images for analyzing nanoparticles' movements. This method enables real-time detection of C-reactive proteins (CRPs) during the rapid interacti...
Conference Paper
Full-text available
A clinical sorting chip integrated with new protein sensing technique has been developed in this work. Antibody-coated nanobeads reacted with antigen was separated from blood plasma based on dielectrophoresis (DEP) forces. MEMS fabrication process was used to produce the bio-chip and only two photo masks were required in the experiment. By using ox...
Article
A particle manipulating micromixer was developed successfully in this study. The present device was fabricated by simply MEMS process. Only two photomasks, one for electrode array chip and another for micro-channel, were needed to manufacture the micromixer. Dielectrophoresis (DEP) force was used to manipulate the particles in the micro-channel. In...
Article
A novel bio-sensing technique based on the measurement of nanobeads' Brownian motion using a micro-particle tracking velocimetry (micro-PTV) has been successfully developed to detect antigen-antibody interactions. The rapid interaction between antigens (C-reactive proteins, CRPs) and nanobeads with conjugated antibodies (anti-CRPs) has enabled real...

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