Yu-Bao Zheng

Sun Yat-Sen University of Medical Sciences · Department of Infectious Diseases

Background/purpose of the study: Mortality from hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF) is high. Severe infection is the most important complication that affects the outcomes of ACLF patients. Thymosin α1 (Tα1) can improve immune imbalance and this study aimed to investigate the safety and efficacy of Tα1 treatment fo...

Objective: To identify markers that predict the progression to hepatitis B virus-associated acute-on-chronic liver failure (HBV-ACLF). Methods: We recruited 125 patients with chronic hepatitis B (CHB) between September 2013 and March 2017. During hospitalization, 25 patients progressed to LF and were classified as the LF group, while the remaini...

Background HIF-1α is relevant to inflammation and fibrosis in hepatitis B virus (HBV)-related liver diseases. Thus, we designed a predictive model for decompensated cirrhosis. Methods Peripheral plasma HIF-1α levels were measured in 52 subjects, including 20 patients with HBV-related-compensated-cirrhosis (HBV-CC), 20 patients with HBV-related-dec...

Backgrounds: Health-care workers' (HCWs) exposure to bodily fluids puts them at risk of hepatitis B virus HBV infection. This study investigated HBV vaccination practices and outcomes in HCWs and assessed postvaccination seroprotection across HCWs in different departments. Methods: A survey of HCWs in a Chinese public general hospital was carrie...

The aim of this study was to screen the non-invasive indexes correlated with liver fibrosis and establish a scoring system for the diagnosis of liver fibrosis in hepatitis B patients. Data of 34 non-invasive indexes were collected for 208 hepatitis B patients. Correlation analysis and stepwise discriminant analysis was used to screen out indexes us...

Background: The pathogenesis of HBV-related acute-on-chronic liver failure (HBV-ACLF) is mainly based on a heightened immune-inflammatory reaction; however, the intimate underlying mechanism remains unclear. Objectives: The aim of the study was to explore potential key immune molecular targets that could serve as early predictive markers for HBV-A...

The aim of this study was to analyze the changes in serum protein levels in the progression of hepatitis B using isobaric tags for relative and absolute quantitation (iTRAQ) analysis, in addition to comparing the serum protein levels of patients with chronic hepatitis B (CHB), patients with hepatitis B virus-induced acute-on-chronic liver failure (...

Objective: The natural history of acute-on-chronic hepatitis B liver failure (ACHBLF) is complex and highly variable. However, the global clinical characteristics of this entity remain ill-defined. We aimed to investigate the dynamic patterns of the natural progression as well as their impact on the outcomes of ACHBLF. Methods: The clinical feat...

Interleukin-6/IL-12 family cytokines play a key role in inflammatory diseases via their effects on the differentiation or regulation of T helper cells. The aim of this study was to determine the role of interleukin-27 (IL-27) and its association with helper T cells in hepatitis B virus (HBV)-infected patients. Samples were assessed from 51 HBV-infe...

Selection of drugs for antiviral therapy of patients with hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF) remains difficult. This study was undertaken to evaluate the short-term efficacy of entecavir versus lamivudine on hepatitis B e antigen (HBeAg)-negative patients with ACLF. The data of 182 HBeAg-negative patients with ACL...

The ability of lymphocytes and macrophage-derived cytokines and chemokines to modulate the activation of stromal cells during immune responses is well-documented, but few studies have investigated whether liver myofibroblasts shape the phenotype and function of monocytes in liver disease. In the present study, Kupffer cells were demonstrated to be...

Purpose The present study was done to establish an objective, sensitive prognostic scoring system and to determine the applicability of this model in predicting the 3-month mortality of patients with acute-on-chronic liver failure in hepatitis B (ACLFB). Methods We developed a novel prognostic scoring system, calculated from six clinical indices i...

To investigate optimal timing for therapeutic efficacy of entecavir for acute-on-chronic hepatitis B liver failure (ACLF-HBV) in hepatitis B e antigen (HBeAg)-negative patients. A total of 109 inpatients with ACLF-HBV were recruited from the Department of Infectious Diseases of the Third Affiliated Hospital, Sun Yat-sen University from October 2007...

Uncontrolled hepatic immunoactivation is regarded as the primary pathological mechanism of fulminant hepatic failure (FHF). The major acute-phase mediators associated with FHF, including IL-1β, IL-6, and TNF-α, impair the regeneration of liver cells and stem cell grafts. Amniotic-fluid-derived mesenchymal stem cells (AF-MSCs) have the capacity, und...

Hepatic differentiation of AF-MSCs and IL-1Ra/MSCs in vitro. (a) The expression levels of specific markers for hepatocyte differentiation (ALB and AFP) were the same in differentiated GFP/MSCs and AF-MSCs. 1: differentiated at day 14; 2: differentiated at day 21. (b) IL-1Ra/MSCs and AF-MSCs possess the same hepatocyte differentiation capacity as de...

Lentiviral vector hIL-1Ra-GFP. pWPXL-MOD(TG-005), Lentiviral vector plasmid containing hIL-1Ra-GFP (11.821 kp). The IL-Ra gene is inserted between the Mlu1 and SpeI sites in the lentiviral vector pWPXL-MOD. (TIF)

Proliferation characteristics, including morphological characterization (a), Growth kinetics (b), and the expression level of Octamer-4 (Oct-4) mRNA in AF-MSCs and IL-1Ra/MSCs (C). (a) Morphological characterization of AF-MSCs and IL-1Ra/MSCs. AF-MSCs and IL-1Ra/MSCs with a fibroblastoid, spindle-shaped morphology; there was almost no difference in...

Primers for β-actin, Oct-4, AFP, and ALB used for RT-PCR. Abbreviations: β-actin, Beta-actin; Oct-4, Octamer-4; AFP, α-fetoprotein; ALB, albumin. (DOC)

The aim of this study was to evaluate the effect of pegylated interferon α-2a plus ribavirin therapy on the quality of life (QOL) of chronic hepatitis C patients when this treatment was paid for by healthcare insurance. The QOL questionnaire (GQOLI-74) was used to assess patient QOL. A total of 42 cases received 1-year pegylated interferon α-2a plu...

The purpose of the current study was to establish an objective, simple, and sensitive prognostic scoring system for estimating the severity of acute-on-chronic liver failure in hepatitis B (ACLFB). A novel prognostic scoring system was calculated from six clinical indices including total bilirubin (TB), prothrombin activity (PTA), creatinine (Cr),...

Unlabelled: Our study aimed to investigate the short-term efficacy and long-term prognosis of liver failure patients caused by hepatitis B after a single transplantation with autologous marrow mesenchymal stem cells (MMSCs). A total of 527 inpatients with liver failure caused by hepatitis B were recruited and received the same medical treatments,...

This study aimed to investigate and compare induced differentiation of mesenchymal stem cells from hepatitis B (HB) patients with liver failure into hepatocytes-like cells with different induction systems in vitro. The differentiation of MSCs from HB patients with liver failure was induced in vitro into hepatocytes-like cells by three cell culture...

Hepatocyte-like cells induced from bone marrow mesenchymal stem cells (BMSCs) recover liver function in animal models with liver failure. Our initial findings revealed that human BMSCs improved liver function in hepatitis B patients with end stage liver disease. However, the susceptibility of BMSCs to HBV infection during induction toward hepatocyt...

Since stem cells can differentiate into hepatocyte, stem cell-based therapy becomes a potential alternative treatment for terminal liver diseases. However, an appropriate source of human mesenchymal stem cells (hMSCs) for hepatocytes has not yet been clearly elucidated. The aim of the present study was to investigate the in vitro biological charact...

The interaction of CD40 with CD154 is a pivotal trigger of immune cascade-inducing acute liver failure, however its clinical significance has not been well studied. The present study aimed to investigate the clinical implications of serum-soluble CD154 (sCD154) levels and the expression of CD40 on monocytes in patients with fulminant hepatic failur...

Ba0.5Sr0.5TiO3 thin films doped with different concentration of Ti, Mg and Al dopants were prepared by pulsed laser deposition technique on LaAlO3 substrates. The crystalline properties of these doped thin films were studied using X-ray diffraction, micro-Raman scattering, atomic force microscopy, and transmission electron microscopy. The bandgap e...

In contrast to metal nanodots, nanoring structures are accessible to various sensing and spectroscopy applications at nanometer scale due to their tunable plasmon resonances and large empty volume confined that provides more spaces for molecular attachment. In almost all the applications, fabrication of large area ordered metal nanoring arrays repr...

The effects of rapid thermal annealing on band structure of HfO2 have been investigated using combinational characterization techniques including X-ray diffraction, X-ray photoelectron spectroscopy and ultraviolet–visible spectrophotometer. The results show that the band structure of the thin film is strongly affected by crystalline structure. Diff...

A simple technique has been developed for fabricating tunable nanostructure arrays, combining ion polishing and nanosphere lithography. Nanohole arrays with the controllable shape and in-plane dimensions are demonstrated. The combination of ion polishing and nanosphere lithography yields tunable masks and provides an easy way for flexible nanofabri...

We have investigated the effect of Ti and Mg dopants on the structural properties and band-gap energies of Ba0.5Sr0.5TiO3 (BST) thin films grown on LaAlO3 substrates. The transmission spectra of these BST thin films measured by ultraviolet-visible spectrophotometer show that the band-gap energies are strongly dependent on the dopant concentration....

Thermal behaviour of ultra-thin Co overlayers on rutile TiO2(1 0 0) surface has been studied by in situ X-ray photoelectron spectroscopy (XPS). Metal Co overlayers of about 30 Å have been deposited at room temperature on rutile TiO2(1 0 0) surfaces, followed by annealing to different temperatures. It was found that the interfacial reaction between...

The Al ₂ O ₃ -incoporation effect on the crystal structure and band structure of Ba _0.5 Sr _0.5 TiO ₃ thin films on (100) LaAlO ₃ substrate has been investigated by x-ray diffraction, x-ray photoelectron spectroscopy, and UV-VIS spectrophotometer. The resultant band gaps (E<sub>g</sub...

Selective molecular-beam epitaxy (MBE) growth of GaAs quantum dots (QDs) on the uniform and periodic arrays of triangle nanocavities bounded by SiO2 mask on Si substrate has been realized. These triangle nanocavities with vertical sidewalls, known as windows, are obtained on Si substrate with thin layer SiO2 on the surface by combining inductively...

Atomic force microscopy (AFM, Nanoscope IIIA) is used to study InAs quantum-dot structures grown by molecular-beam epitaxy (MBE) on a shallow spherically shaped crater (also called a dimple) with the continuous polar misorientation and azimuthal orientation prepared on GaAs (001) substrate by a Model 656 Dimple Grinder. It is shown that the step bu...

As a potential alternative treatment for terminal liver diseases, amniotic fluid derived mesenchymal stem cells (AFMSCs) have many advantages over other stem cells: avoiding much ethical controversy and decrease in both quantity and differentiation potential with age. However, the therapeutic role of AFMSC for fulminant hepatic failure (FHF) has no...