Youn-Joo An

Konkuk University | KU · Department of Environmental Health Science

Owing to their ubiquitous nature, microplastics are a major environmental concern. This study reviewed the toxicity data of microplastics in marine water, and analyzed their species sensitivity distribution (SSD) curves and hazardous concentrations (HCs). Toxicity database of no-observed effect concentration (NOEC), 50% effect concentration (EC50),...

Tire particles (TPs) generated on roads are a main contributor to microplastic environmental pollution. In this study, TP leachates from three vehicle types (bicycle, car, and electric scooter) were prepared. TP leachate toxicity impacts on three organisms (Vigna radiata, Daphnia magna, and Danio rerio) were analyzed, in addition to their chemical...

Soil quality assessment is generally performed using a combination of physical, chemical, and biological indicators in a target area, and the indicator measurement data are subjected to sequential processes such as scoring, weighting, and indexing. The selection of indicators is one of the important processes, and their actual application should be...

The practice of reusing biosolids may be an effective strategy for addressing nutrient reuse and soil shortages. However, the accumulation of silver nanoparticles (AgNPs) in biosolids poses a major ecological hazard to organisms. In this study, we evaluated the effects of AgNPs on plant and soil enzymes in terrestrial ecosystems by exposing soybean...

The use of facial masks has increased and is therefore being recognized as a large source of environmental microplastics. Herein, we naturally aged disposable masks in a lake for eight weeks and compared the toxicity of mask-derived microplastics depending on the aging process using zebrafish (Danio rerio). Zebrafish were exposed to virgin and aged...

Although the springtail Folsomia candida is a representative soil test species and a widely used biological indicator, current toxic endpoints are limited and required to expand at the individual level. For instance, there is no simple and efficient method for evaluating gut barrier damage in Folsomia candida except for histopathological evaluation...

Micro- and nanoplastics are released into the soil through various anthropogenic activities; however, research on ecological risk assessment (ERA) of soil microplastics is limited. In this study, the species sensitivity distributions (SSDs) of representative groups of soil biota were analyzed to determine their sensitivity to microplastic propertie...

The 2019 global coronavirus disease pandemic has led to an increase in the demand for polyethylene terephthalate (PET) packaging. Although PET is one of the most recycled plastics, it is likely to enter the aquatic ecosystem. To date, the chronic effects of PET microplastics (MPs) on aquatic plants have not been fully understood. Therefore, this st...

Currently, there are no standard international test methods for assessing aquatic and soil toxicity, with aquatic toxicity tests based on limited Euglena species. Here, we proposed Euglena species as extended test species, especially as new soil test species for a paper-disc soil method, considering its ecologically important roles in providing hig...

Biotic ligand models (BLM) and the sensitivities of indigenous species are used to assess the environmental risk considering the bioavailability of metals, such as nickel. However, the BLM‐based acute‐chronic ratio (ACR) is required, if the predicted no‐effect concentration (PNEC) cannot be derived from the chronic species sensitivity distribution...

This study evaluated the size-dependent effects of high-density polyethylene (HDPE) fragments in zebrafish. Larvae were exposed to HDPE microplastic (MP) in three sizes, small (14.12 μm), medium (80.32 μm), and large (120.97 μm), at 20 mg/L. Size-dependent effects in terms of MP intake, subsequent gut damage, and behavioral changes were observed. T...

This study proposes a microplate assay for chemical toxicity evaluation using S. natans; this assay is a miniaturization of a culture dish assay from previous studies. The optimal initial frond number of S. natans in the microplate assay was established and the feasibility of the microplate assay was evaluated. To evaluate the applicability of the...

Microplastics (MPs) and ultraviolet (UV) filters cause pollution in aquatic ecosystems. Moreover, regulations on the use and discharge of UV filters in personal care products are lacking. Therefore, the combined toxicity of MPs (virgin polystyrene (PS) spheres; size: 200 nm) and avobenzone (AVO; a UV filter) on Daphnia magna were assessed. The expo...

Objectives : Since the risk of pollutants in soil environment may vary depending on the characteristics, functions, and environmental conditions of the soil, the site-specific soil ecological risk assessment (SERA) should be conducted to protect soil ecosystem from pollutants. It has been confirmed that each country is conducting SERAs based on sit...

Comparative toxicological assessment studies on the effects of petroleum- and cellulose-based microfibers on aquatic plants are limited. Therefore, we evaluated the acute and 10-generational toxicological effects of two types of petroleum- and cellulose-based microfibers on the duckweed Lemna minor. Plant growth and photosynthesis parameters were m...

Ethylparaben is used as an antifungal preservative. Although some countries have implemented regulations for human exposure to parabens, environmental regulations for ethylparaben have not been established. This study provides new toxicological data for ethylparaben, for which data regarding soil organisms were previously lacking. Although ethylpar...

This study investigated the effects of nanoplastics on marine organisms via trophic transfer in the food chain. We designed a three-step food chain comprising microalga (Dunaliella salina), small crustaceans (Artemia franciscana), and fish (small yellow croakers; Larimichthys polyactis) and evaluated the effects of trophic transfer in marine organi...

Microfibers are widespread environmental pollutants introduced to the soil environment because of the increasing use of plastic polymers. However, research on the soil ecotoxicity of microfibers is limited, especially when compared to research on their aquatic toxicity. This study investigated the effects of sub-chronic microfiber exposure on the e...

Tire particles are generated by the abrasion of tire treads on roads and are major contributors to microplastics in soil environments. Contamination by tire wear particles worsens annually as the use of personal mobilities increases. Tire particles (112-541 µm) were obtained from three types of personal mobility tires (bicycle, car, and electric sc...

Although plastics are widely distributed in soil environments, few studies have assessed their effects on different test species. In this study, the responses (adsorption or internalization) of two green algal species (Chlorococcum infusionum and Chlamydomonas reinhardtii) to nanoplastics in soil were evaluated via microscopic analysis for nine wee...

Microplastics (MPs) released into soil environments, along with the existing pollutants in soil, may have adverse effects on plants. However, the chronic effects of MPs in soils contaminated with heavy metals on crop plants remain unidentified. This study aimed to investigate the chronic effects of MPs (polystyrene, 20 nm) on the reproductive and n...

The paper-disc soil method is a soil algal bioassay used to assess soil water transfer in polluted soils. Although this ecotoxicological method was first established in 2016 to assess soil pollution, quantitative toxicity assessments of Cu- or Ni-treated soil and in situ or ex situ soils polluted with other heavy metals (HMs) have not been widely c...

As a result of the efforts to introduce the principle of the 3Rs (replacement, reduction, and refinement) into animal testing, alternative in vitro skin corrosion test methods have been developed and standardized globally. However, alternative in vitro skin corrosion test methods have some limitations in terms of the use of humanely killed rats or...

Ecological risk assessment based on scientific data is crucial for understanding causal relationships between chemical pollution and environmental risks. Simultaneously, a balance is required between socioeconomic factors and scientific evidence. The TRIAD approach, which incorporates three lines of evidence (LoE)—chemical (Chem-LoE), ecotoxicologi...

Due to pervasive and resilient soil contaminants, heterogeneously contaminated soil poses unpredictable potential threats to ecosystems. In this study, the extension of a previously developed soil algae pipe assay for evaluating heterogeneously contaminated soil under an open system is described. The assay can be used in soil that is heterogeneousl...

This study investigated the trophic transfer of nanoplastics in marine food chains. We fed nanoplastic-exposed Artemia franciscana (brine shrimp) to Larimichthys polyactis (small yellow croaker) daily for eight days. Subsequently, the overall health condition, histopathological damage to the liver and digestive tract, and swimming ability of the fi...

Shoe sole fragments are generated by sole abrasion, which is unavoidable. These fragments can enter the soil ecosystem. However, limited studies have evaluated their effects on soils and plants. Here, we aimed to evaluate the toxicity of shoe sole fragments on a crop plant, Vigna radiata (mung bean). Shoe sole fragments (size: 57–229 μm) were obtai...

This study investigated the toxic effects of leachates from microplastic fragments of soles from four different types of shoes (slippers, trekking shoes, running shoes, and sneakers) on three aquatic organisms (Chlamydomonas reinhardtii, Daphnia magna, and Danio rerio). The chemical components in each leachate were identified; furthermore, chloroph...

When considering test species for soil ecotoxicity, the development of new model organisms is often suggested to increase the reliability of ecological risk assessments. Ubiquitous soil algae could offer potential test species for assessing various soil pollution levels. Currently, there are few reviews offering comprehensive perspectives on stress...

Toluene is a highly flammable and commonly used industrial chemical with severe health consequences on humans upon exposure and ingestion. In this study, multispecies bioassays were conducted using a species sensitivity distribution approach to determine acute and chronic hazardous concentrations of toluene in soil. Acute and chronic toluene toxici...

Microfibers, as globally emerging environmental pollutants, have received increasing attention over recent years. In ecosystems, natural textile microfibers are the predominant fibers. The inadvertent ingestion of microplastics by organisms in an ecosystem provides a channel for microfibers to enter biological webs. Based on existing research on mi...

Plastics are ubiquitous and persistent in aquatic environments, threatening environmental and human health. This review focused on the effects of plastics (single toxicity) and associated chemicals (combined or leachate toxicities) on freshwater and marine microalgae. Forty-seven publications from 2010 to 2020 were used in this review. Based on the...

Microfibers, which are sourced from textiles and some products from the fishery industry, are the biggest contributors to microplastic pollution in aquatic ecosystems. In addition to these synthetic microfibers, naturally derived microfibers can also be found in aquatic environments. However, there are limited studies on the ecotoxicity of natural...

Microplastics are ubiquitous environmental pollutants and a great threat to the aquatic environment. Due to their small size (ranging from 1 μm to 5 mm), microplastics be easily ingested by a wide range of organisms and can serve as a vector for various contaminants. The effects of microplastic (< 2.0 μm) ingestion have been investigated in various...

Pb-based perovskite in solar cells is a source of PbI2. The objective of this study was to characterize the embryonic toxicity of PbI2, a potentially leachable chemical and hazardous material, for two fish species (zebrafish and Japanese medaka). A series of measurements were performed to assess mortality, abnormalities (deformities and other patho...

Globally, perovskite solar cells (PSCs) represent a third-generation photovoltaic technology that is being increasingly implemented and commercialized. However, the biological impacts of leachates from PSCs are poorly understood. Therefore, the aim of this study was to investigate the ecotoxicity of PSC leachates compared with that of commercial Si...

For site-specific soil ecological risk assessments (SERAs), an integrated chemical, ecotoxicological, and ecological analysis needs to be performed. The SERA guidelines of international institutions and countries recommend that a SERA be initiated at the screening level to save time and social economic cost; however, they provide no unified test sp...

Because of the COVID-19 pandemic, used face masks have increasingly littered the environment and are causes for concern since they are commonly made of plastics such as polypropylene. Understanding production of microplastics from face masks is essential for predicting the post COVID-19 pandemic impact on the soil ecosystem. We investigated the gen...

Various pollutants are pervasive in soil environments due to human activities, thereby damaging soil ecosystems. In this study, extension of a previously developed algae-soaked disc seeding assay for periodic evaluation of subsoil contamination over time was described. The assay can be used in different contamination configurations of silver nanopa...

Microplastics are widespread contaminants in soils and terrestrial ecosystems in many areas worldwide. In this study, we measured soil organic carbon (SOC) and soil organic matter (SOM) in microplastic-treated soils to determine if the presence of microplastics could affect the accuracy of carbon-based soil quality indicator measurements. Six diffe...

The copper biotic ligand model (BLM) can quantitatively describe the bioavailability depending on various environmental factors and has been used to derive the predicted no-effect concentrations (PNECs). The commonly employed acute BLM tool, HydroQual, which applies the biotic ligand constants of fathead minnow in the same model structure for all t...

Vitellogenin has been regarded as an acceptable indicator for evaluating the endocrine-disrupting property of chemicals using fish. However, the endocrine-disrupting property of chemicals has been rarely evaluated using soil species. This study aimed to find evidence that endocrine-disrupting chemicals (including the natural hormones estradiol and...

The increasing amount of microplastics in aquatic ecosystems is a significant environmental issue, with adverse effects on marine organisms including invertebrates and vertebrates. This study examined the effects of three types of microfibers on the brine shrimp Artemia franciscana as the test species. The brine shrimps were exposed to two commonly...

Despite concerns about the ecotoxicological effects of microplastics in soils, there is a limited understanding of the reproductive toxicity of microplastics to soil organisms and the production of nanoplastics through biological fragmentation. We used the earthworm Eisenia andrei to investigate the generation of nanoplastics from polyethylene micr...

Objective:As the industry rapidly develops in modern society, the use of chemicals increases and people and various organism living in terrestrial environments are exposed to these chemicals in large quantities. Chemical accidents frequently occur around the world, and these accidents could cause fatal damage to the terrestrial ecosystem. When the...

The use of hazardous metals like lead, cadmium in solar photovoltaics (PVs) are rapidly increasing which poses the risk to the environment due to potential release of these constituents. The main purpose of this review is to highlight the updated information on solar PV waste along with the present condition of efforts for recovery, country-wise re...

Although macrophytes are known to play vital roles in aquatic ecosystems, most quantitative aquatic toxicity data focus on fishes, water fleas, or algae, with limited ecotoxicity data published on macrophytes. Salvinia natans is a fast-growing plant commonly found in freshwater habitats. In this study, we verified a suitable disinfectant for preven...

Microfibers (MFs) have recently become an increasingly prevalent pollutant in ecosystems and pose a direct threat to organisms and an indirect threat via adsorption of other pollutants. Here, we used Caenorhabditis elegans to study multigenerational effects of polyethylene terephthalate (PET) MFs (diameter 17.4 μm) by observing the maternal generat...

2,4-dinitrophenol (2,4-DNP) is a phenolic compound used as a wood preservative or pesticide. The chemical is hazardous to freshwater organisms. Although 2,4-DNP poses ecological risks, only a few of its aquatic environmental risks have been investigated and very limited guidelines for freshwater aquatic ecosystems have been established by governmen...

Despite their many advantages, solar photovoltaic (PV) cells used for electricity generation can have negative environmental impacts. The chemicals necessary for their fabrication can be released into the environment during their disposal or following damage, such as that from natural disasters. The principle objective of this study was to assess t...

Many reliable studies have provided evidence of microplastic ingestion by soil organisms. However, further research is required to determine the edible size of microplastics, especially given the ubiquity of microplastics and their adverse effects on the soil environment. Determining the size range of microplastics that can be ingested by soil orga...

The deterioration of soil quality owing to human activities results in adverse effects on the soil ecosystem. This study developed a systematic method to quantitatively evaluate soil quality based on physical, chemical, biological, and ecotoxicological indicators and proposed the soil quality assessment and management system. This system consists o...

Phenol is widely used in many industries, and chemical accidents involving phenol have frequently occurred around the world, resulting in the investigation of phenol toxicity in humans, mammals, and aquatic organisms. However, very few studies have investigated phenol toxicity in terrestrial ecosystems. Therefore, we investigated the acute and chro...

Objective With rapid industrial development, human and the environment are frequently exposed to various chemicals. In particular, there is a possibility that terrestrial ecosystems as well as humans are exposed to a large amount of highly toxic chemicals due to accidents occurring frequently in Korea and abroad. If a large amount of chemicals ent...

Environmental and human health challenges are pronounced in Asia, an exceptionally diverse and complex region where global megatrends are extensive and numerous stresses to environmental quality exist. Identifying priorities necessary to engage grand challenges can be facilitated through horizon scanning exercises, and to this end we identified and...

Propylparaben is widely used as a preservative in pharmaceuticals and personal care products and is ultimately excreted by the human body. Thus, propylparaben reaches sewage and enters the soil environment by sludge fertilization and wastewater irrigation. However, there are few existing studies on the toxicity and risks of such chemicals in terres...

Recently, numerous studies concerning dye-labeled microplastic beads have reported on the end-of-life, environmental effects of microplastics because of their ubiquitous commercial usage. Less is understood about the toxicity and bioaccumulation of plastics other than microplastic beads, which can also harm the environment (e.g., fragments, fibers,...

This study investigated the transfer of plastic debris in a terrestrial environment from the soil to a plant (the mung bean, Vigna radiata), and then to a consumer (the African giant snail, Achatina fulica). The adverse effects of these plastic pollutants on the physiology of the plant and animal were investigated. The mung bean plants were directl...

Hydrogen peroxide (H2O2) is a strong oxidizer and bleaching agent included in the list of substances requiring accident preparedness by the National Chemical Information System, Korea. Although chemical accidents related to H2O2 frequently occur globally, few studies have evaluated its toxicity and risk to soil ecosystems. Herein, accidental exposu...

Plastic wastes are widespread pollutants in marine environments and several studies have focused on their impacts on different ecosystems. Microplastics (MPs, < 5 mm) have been the focus of a particularly extensive investigation because of their ubiquity, large surface area, interactions with organisms, and the challenges they present in terms of d...

Due to its widespread use, large amounts of expanded polystyrene (EPS) have been released into the marine environment, where it is broken down into small pieces with large surface areas. As such, chemical additives may be released into the environment, which can affect marine organisms; however, studies of the effects of such additives are lacking....

Photovoltaic (PV) technology such as solar cells and devices convert solar energy directly into electricity. Compared to fossil fuels, solar energy is considered a key form of renewable energy in terms of reducing energy-related greenhouse gas emissions and mitigating climate change. To date, the development and improvement of PV technologies has r...

Contamination of the world's oceans with plastic waste has attracted increasing attention in recent years. Whereas the ecological consequences of plastic pollution have been the focus of increasing research, the health-related implications of plastic pollution have been somewhat overlooked. In this study, we exposed whiteleg shrimp (Litopenaeus van...

Perfluorooctanoic acid (PFOA) is one of the persistent organic pollutants that has been listed in Annex A of the Stockholm Convention and has attracted attention owing to its endocrine-disrupting properties. However, there is currently little information available regarding the soil ecotoxicity of PFOA and the associated ecological risks. According...

There is increasing concern about how microplastics (MPs) are impacting marine ecosystems. In particular, studies on how MPs impact microalgae are required because of the abundance of MPs and importance of green microalgae as primary producers. This study investigated how MPs that are larger (200 μm) than algal cells impact them, using the marine m...

In the present study, we evaluate our previously developed non-destructive soil algal toxicity method using species from a different class of algae; Class Trebouxiophyceae (Chlorella vulgaris and Chlorella sorokiniana), and directly measure the photosynthetic activity of these species adsorbed onto the soil as a new toxicity endpoint. This study sh...

Previous research on soil remediation focused on soil properties and not on its effects on soil ecosystems. The present study investigated the adverse effects of soil physicochemical changes due to remediation on the biological indicators Chlorococcum infusionum and Chlamydomonas reinhardtii (algae) and Eisenia andrei (earthworm). Soil physicochemi...

Previous studies have indicated the means by which micro-sized plastic particles may affect the soil environment, and this could be linked to the behavior of plastics in the soil system and how these particles are influenced by biological responses. Soil-dwelling organisms play a key role in modifying the soil system by constructing bio-pores, and...

Recently, Korean consumers have experienced public health issues as a result of problematic feminine hygiene products. Consumers and local civic groups have urged manufacturers and the Korean government to provide clear information based on comprehensive studies and strengthen related policies. In this study, we collected and compiled information o...

Nonylphenols are endocrine-disrupting chemicals that are used in various industries and are constantly discharged into the terrestrial environment. However, there have been few studies on the phytotoxicity of this chemical in the soil environment. In this study, mung bean (Vigna radiata) and rice (Oryza sativa) were grown in soil containing nonylph...

We aimed to develop an ex situ method for evaluating soil quality using soil algae. The soil algae pipe assay involves application and sampling of algae and flow cytometry. As it can be performed in a greenhouse, the assay was effective for pot tests and conducive for soil quality assessment. This novel assay will enable broader toxicity testing.

Silver nanomaterials (AgNMs) of different shapes and sizes are potentially toxic to aquatic organisms. However, studies on the toxicity of AgNMs and on their shape-dependent effects on algae are scarce. The present study evaluated the effects of three AgNMs (silver nanospheres, AgNPs; silver nanowires, AgNWs; silver nanoplates, AgPLs) with differen...

In this study, we investigated multi-generational effects and generation particle transfer in Caenorhabditis elegans following maternal food exposure to core-shell quantum dots. We found that that the Bag of Worms (BOW) phenotype in aged worms induces changes in quantum dot distribution in the parental body, which is related to the inter-generation...

Silver nanomaterials (AgNMs) of various shapes and sizes are manufactured for different purposes and used as ingredients in a wide variety of products and applications. Recently, the toxicity of AgNMs has attracted significant attention. However, the effect of the shape of AgNMs (particles, wires, plates) on their toxicity in soil ecosystems remain...

Di (2-ethylhexyl) phthalate or DEHP is classified as an endocrine-disrupting chemical. It is used as a plasticizer and pesticide additive. Moreover, it has a half-life of about 150–300 days. Thus, it is present in the soil environment and soil risk assessments for DEHP are needed. However, a number of studies have focused on the effects of DEHP in...

Methyl ethyl ketone (MEK) is a common and widely used industrial solvent. However, few studies have investigated its toxicity, or its effects as a contaminant in soil ecosystems. In this study, acute and chronic toxicity data for MEK were generated, and ecological risk based on a species sensitivity distribution was assessed. Seven soil organisms f...

This study investigated the ingestion behavior of zebrafish exposed to microplastic particles (MPs) at different concentrations, presented alone or in a mix with food particles. Zebrafish showed spitting behavior after ingesting micro-sized (247.5 μm) polyethylene particles (i.e., MPs), with prey-capture time increasing when food and MPs were suppl...

Methylparaben, which is known to be an endocrine-disrupting chemical, is added to various personal care products, including cosmetics, and is also used as a food preservative and in pharmaceuticals. However, information on the toxicity of methylparaben in soil ecosystems is limited. Furthermore, unlike other substances such as metals and pesticides...

Microplastics (MPs) have adverse effects on aquatic organisms in marine environments; however, there is a lack of information on freshwater environments. This study investigated the dietary uptake, and biodistribution and depuration of MPs in the freshwater diving beetle Cybister japonicus (Coleoptera: Dytiscidae) after consumption of zebrafish (Da...

Pesticide residue in the eggs in the market has become a significant problem in Korea. In particular, the detection of fipronil indicated that unregulated pesticide residue can be present in livestock products, including eggs. Institutionally unregulated pesticides are a risk to human health and the ecosystem. In this study, we analyzed the system...

Daphnia galeata and Daphnia magna belong to the family Daphniidae. Daphnia galeata has a smaller body size and longer helmet than D. magna. Although D. galeata is widely distributed in the northern hemisphere, it is not as commonly used in aquatic ecotoxicity tests as D. magna. There have been only few ecotoxicological studies on the toxicity of he...

The compound bisphenol A (BPA), an endocrine-disrupting compound that can act as an estrogen, is widely used in the industrial manufacture of plastic products and epoxy resins. Because of the widespread use of the compound and its use in soil amendments, there is concern regarding its effects on crop plants, although comparatively little informatio...