Yoshimasa Sugiura

National Fisheries University · Department of Food Science and Technology

There are conflicting animal experiments on the effect of trimethylamine N-oxide (TMAO), the dietary metabolite, on non-alcoholic fatty liver disease (NAFLD). This study aims to determine the effect of TMAO on NAFLD. A diet containing 0.3% TMAO was fed to farnesoid X receptor (Fxr)-null mice, a model of NAFLD, for 13 weeks. Fxr-null mice fed TMAO s...

The functional properties of edible lavers are well-known; however, there is limited information on the marine red alga Pyropia tenuipedalis, an indigenous species found in the Yamaguchi Prefecture area. In the present study, samples of P. tenuipedalis were collected from the Koto River Estuary and cultivated using land-based cultivation techniques...

We investigated the antiallergic effects of 80 % methanol (80M) extracts obtained from Ecklonia cava subsp. stolonifera, harvested along the coast of Nishinoshima Town, Oki Islands, Shimane Prefecture, Japan, at different stages of algal powder manufacturing (involving heating operations). The radical-scavenging activity of the extracts increased d...

The antiallergic properties of phlorotannins, algal polyphenols, have been widely reported. This study examined the soothing effect of phlorotannin concentrate (PTC) from Eisenia nipponica on cedar pollinosis in Cry j 1-stimulated mice. PTC reduced the mice's sneezing and nasal rubbing, which was attributed to decreased levels of immunoglobulin E a...

Histamine (HIST) and other biogenic amines found in fish and fishery products accumulated by the action of bacterial amino acid decarboxylase cannot be decomposed and eliminated by heating or other chemical methods. A simple method for HIST elimination is proposed by a coupling reaction of the fungal amine oxidase (FAO) and bacterial aldehyde oxida...

The immunomodulating effect of phlorotannin was investigated in mice stimulated by ovalbumin. When analyzing the main components of phlorotannin concentrate (PTC) from Eisenia nipponica, seven phlorotannins [eckol, 6,6′-bieckol, 6,8′-bieckol, 8,8′-bieckol, dieckol, phlorofucofuroeckol (PFF)-A, and PFF-B] were detected. These phlorotannins accounted...

Because the number of people suffering from allergies has significantly increased, improved ways of treating these conditions by medical, pharmaceutical, and dietary means are required. Large numbers of studies on allergy have been conducted, and many anti-allergic compounds have been found. Phenolic compounds from terrestrial plants, including cat...

Here, we investigated the antiallergic effects of brown algae (Ecklonia cava subsp. stolonifera) harvested on the coast of Nishinoshima Town, Oki Islands, Shimane Prefecture, Japan. Specifically, we investigated whether the active components of E. cava ssp. stolonifera vary seasonally and whether such variation correlates with seasonal variation in...

Studies using animal models of hypercholesterolemia have established that taurine reduces cholesterol levels; however, the precise mechanism underlying this cholesterol-lowering effect is unclear. This study addressed this issue by investigating whether bile acid/farnesoid X receptor (FXR) signaling is involved in taurine-mediated cholesterol-lower...

We previously found a lipophilic fraction of the methanol/chloroform extract of a brown alga, Eisenia nipponica, that had an antiallergic effect in a murine ear swelling test. In this study, we purified the active component from the lipophilic fraction using high performance liquid chromatography and analyzed the mass and nuclear magnetic resonance...

Selenoneine is a novel organic selenium compound markedly found in the blood, muscles, and other tissues of fish. This study aimed to determine whether selenoneine attenuates hepatocellular injury and hepatic steatosis in a mouse model of non-alcoholic fatty liver disease (NAFLD). Mice lacking farnesoid X receptor (FXR) were used as a model for fat...

Mice lacking the farnesoid X receptor (FXR) are used as a genetic model for nonalcoholic fatty liver disease because their livers exhibit hepatic steatosis and inflammation. The influence of taurine drinking on disrupted hepatic function was investigated using female Fxr-null mice. Significant decreases in the levels of hepatic damage-associated di...

This study attempted to culture koji mold Aspergillus oryzae on seaweeds including Pyropia yezoensis, Sarugassum horneri, Saccharina japonica, Ecklonia stolonifera, Eucheuma muricatum, and Ascophyllum nodosum. The seaweeds were added with water, starter strains of A. oryzae, and incubated for 3 days at 30-34.5℃. The mold growth was observed for all...

The influence of fish oil-enriched Sasa-kamaboko (a Japanese processed seafood) diet on lipid metabolism was investigated using mice lacking farnesoid X receptor (FXR) as a fatty liver disease model. Sasa-kamaboko (SK) was made from Alaska pollock surimi (fish paste), enriched with fish oil (0%, 2.5%, or 5.0%) and then freeze-dried. Fxr-null mice w...

Phlorotannin is the collective term for polyphenols derived from brown algae belonging to the genera Ascopyllum, Ecklonia, Eisenia, Fucus and Sargassum etc. Since the incidence of allergies is currently increasing in the world, there is a focus on phlorotannins having anti-allergic and anti-inflammatory effects. In this study, six purified phlorota...

Mice lacking farnesoid X receptor (FXR) are used as a model for nonalcoholic fatty liver disease because their livers exhibit hepatomegaly, hepatic steatosis, and hepatic inflammation. The influence of fish oil feeding on hepatomegaly and disrupted hepatic function was investigated using female Fxr-null mice and wild-type mice fed a fish oil diet (...

The anti-inflammatory effects of two phlorotannins identified in the brown alga Eisenia arborea, i.e., 6,6′-bieckol and 6,8′-bieckol, were previously determined in vitro. Here we investigated the in vivo effects of these phlorotannins in mice. In ICR mice, ear swelling was induced by three sensitizers: arachidonic acid, 12-O-tetradecanoylphorbol-13...

To evaluate the correlation between the commercial grade determined by organoleptic judgment panel and chemical substances in dried laver Porphura spp., we analyzed the contents of free amino acids, 5′-nucleotides, total lipids, fatty acids, α-tocopherol, lipophilic pigments, and aldehydes in several grades of laver that had been classified by an o...

Mice lacking farnesoid X receptor (Fxr-null mice) are used as a model of non-alcoholic fatty liver disease because they exhibit higher levels of hepatic lipids and hepatic damage-associated diagnostic markers. The influence of hepatic accumulation of n-3 polyunsaturated fatty acid (PUFA) was investigated using Fxr-null mice fed either a corn oil (4...

Effects of a short chilled storage period before freezing, frozen storage temperature, and freezing rate on lipid oxidation of bluefin tuna (Thunnus thynnus) meat during frozen storage were investigated. After 12 months storage, all samples had increased in peroxide value though they were less at the lower temperatures (-45°C and -60°C). Peroxide v...

This study investigated the anti-allergic effect of a diethyl ether fraction from dried Sargassum fusiforme. In vivo experiments showed that percutaneous administration of this fraction suppressed mouse ear swelling induced by three types of sensitizers (arachidonic acid, 12-O-tetradecanoylphorbol-13-acetate and oxazolone). At a dose of 0.1 mg/mous...

The anti-inflammatory effects of fucoxanthin (Fx) were investigated in vivo and in vitro. When mouse ear swelling was induced by three sensitizers, arachidonic acid, 12-O-tetradecanoylphorbol-13-acetate and oxazolone, both percutaneous and oral administration of 150 nmol/mouse of Fx suppressed the effects, similar to a typical natural inhibitor, ep...

Tropomyosins are defined as risk factors for shrimp allergy. However, their concentration in different preparations has not been clarified. We quantified the tropomyosin concentration in shrimp meat, which was cooked using several methods or was stored under various conditions. The results demonstrated that shrimp meat from various preparations and...

Effects of a freezing period and frozen storage temperature on lipid oxidation of frozen Atlantic mackerel (Scomber scombrus Linnaeus) and frozen Chub mackerel (Scomber japonicus Houttuyn) during frozen storage were investigated. The lipids contents of frozen Atlantic mackerel captured from Norway and frozen Chub mackerel captured from low latitude...

To determine the anti-allergic effects of Ecklonia kurome, the ethyl acetate fraction from an E. kurome (EEK) extract was compared to the ethyl acetate fraction from an Eisenia arborea (EEA) extract and epigallocatechin gallate (EGCG) for inhibitory effects on inflammation in experimental animals, degranulation in inflammatory lymphocytes and enzym...

Hydroxy lipids (L-OH) and 4-hydroxy-2-hexenal (HHE) levels as well as other parameters such as lipid level, lipid class, fatty acid composition, and other aldehydes levels in the liver of diseased fish were investigated. Although significant differences in lipid level, lipid class, fatty acid composition, and other aldehyde levels were not always o...

Changes in hydroxyl lipids (L-OHs; a stress level indicator), body weight, triglycerides, fatty acids, and free amino acids (FAAs) were investigated in Decapterus maraudsi caught by purse seine from two different ocean regions near Japan to determine the effect of short-term holding without feeding on the reduction in oxidative stress. In experimen...

The anti-allergic and anti-inflammatory effects of phlorotannins (seaweed polyphenols) from the brown alga Eisenia arborea using in vitro experiments has already been reported. Therefore, in this study, these effects were examined in vivo using mice. When ear edema was induced in ICR mice by three sensitizers (arachidonic acid (AA), 12-O-tetradecan...

Skinless fillets were prepared from Pacific saury Cololabis saira of varying degrees of freshness and then stored at −20 and −40C for 12 months to investigate the effects of lipid oxidation on quality deterioration. Peroxide value and 4-hydroxy-2-hexenal levels increased with storage temperature (−40 < −20C) and corresponded with freshness. Peroxid...

In this study, we developed a reversed-phase high-performance liquid chromatographic method with fluorometric detection for the simultaneous quantitation of 4-hydroxy-2-hexenal (HHE) and 4-hydroxy-2-nonenal (HNE) in foods by using 1,3-cyclohexanedione reagent. This method provides good resolution and reproducibility. Chromatography is conducted wit...

Ibaranori (Hypnea charoides), a red alga, grows along the coast of the Japanese islands and is commonly eaten in southwestern areas such as Okinawa Prefecture. While H. charoides is thought to be a health-promoting food, the underlying mechanism of this benefit is not well understood. In this study, in order to examine the health benefits of H. cha...

The anti-allergic effects of the edible brown alga, Ecklonia stolonifera, are known to affect both degranulation in KU812 cells and anti-inflammatory properties of RAW 264.7 cells. Additional anti-allergic effects of E. stolonifera extracts were therefore investigated by examining the inhibitory effects of algal extracts on enzymes associated with...

The intestinal absorption of three free amino acids contained in various seafood extracts, i.e. taurine, ornithine and glycine, and the effects of capsaicin treatment on their absorption were invastigated using a rat in situ intestinal loop method. The order of velocities of intestinal absorption was as follows: glycine ~ ornithine > taurine, and t...

The anti-allergic effect of an edible brown alga, Eisenia arborea, was investigated by determining the anti-degranulation activity on RBL cells or KU812 cells and the inhibitory effects on enzymatic activities involved in eicosanoid (leukotoriene and prostaglandin) synthesis. When the stimulated cells were treated with a M/C (methanol:chloroform=1:...

The anti-allergic effects of commercial products from cooked burdock (burdock salad, fried up burdock and burdock boiled with vinegar) were investigated by analyzing their inhibitory effects on the enzymatic activity (cyclooxygenase-2, lipoxygenase, phospholipase A2 and hyaluronidase) and the suppression of degranulation from rat basophilic leukemi...

Six anti-allergic phlorotannins from the brown alga Eisenia arborea were examined for their inhibitory effects on oxidation and hyaluronidase activities. To investigate the radical scavenging activities of the phlorotannins, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was evaluated. Although the radical scavenging activity...

To investigate the anti-allergic effects of the brown alga Eisenia arborea. A strain of Brown Norway rats known to strongly respond to immunoglobulin E (IgE) were used as an allergy model animal. The rats were immunized with ovalbumin by oral administration. The levels of serum IgE and histamine were suppressed in the rats fed a diet supplemented w...

Eisenia arborea, an edible brown alga, is occasionally used as a folk medicine due to its anti-allergic effect. In the present study to identify the anti-allergic constituents in the alga, the extract of the alga was purified by partition between solvents and by reversed phase chromatography. Separation of the extract was guided by the inhibitory a...

The effects of 80% methanol extracts from frozen samples of 41 macroalgae and one sea grass collected in the Ise-Shima region of Japan were investigated on histamine release from rat basophile leukemia cells (RBL-2H3) sensitized with antidinitrophenyl (DNP) IgE and stimulated with DNP-BSA. Of the 21 brown algae, five green and 15 red algae, and one...

Eisenia arborea is an edible brown alga occasionally used as a folk medicine in gynecopathy in Japan. A new phlorotannin was isolated from the alga during our search for naturally occurring anti-allergic compounds from edible algae guided by the inhibitory effect on histamine release from rat basophile leukemia (RBL)-2H3 cells. The phlorotannin was...

Potent inhibition of cell proliferation was found for commercial preparations of bovine alpha-lactalbumin on cultured intestinal cell line IEC-6 albeit lot-dependent. The inhibition was irreversible and a single exposure to the culture medium containing alpha-lactalbumin of an active lot for a period as short as 30 min was enough to provoke cell de...

Monomeric 14-kDa bovine alpha-lactalbumin was purified with a preparation of lower molecular weight whey protein concentrate from Holstein cow normal milk followed by size exclusion chromatography. The protein showed a stimulatory rather than an inhibitory effect on the proliferation of a cultured IEC-6 cell line from the rat small intestine. But i...