Yong Wang

Yong Wang
Lanzhou University of Technology · School of Life Science and Engineering

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60
Publications
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373
Citations

Publications

Publications (60)
Article
Full-text available
To better understand the function of transporter in heavy metal detoxification of bacteria, the transporters associated with heavy metal detoxification in S. rhizophila JC1 were analyzed, among which four members were verified by RT-qPCR. In addition, the removal rates of four single metal ions (Cr⁶⁺, Cu²⁺, Zn²⁺, Pb²⁺) and polymetallic ions by stra...
Article
High-throughput sequencing was used to study the microbial community structure diversity changes in oil-contaminated soils under different spatial distances and environmental conditions. 239 Phyla, 508 Classes, 810 Orders, 1417 Families, 2048 Genera, 511 Species of microbial communities were obtained from 16 samples in three regions. The physicoche...
Article
In this study, we focused on the plant-growth-promoting properties of two strains isolated from Codonopsis pilosula, and the effect of inoculation with different strain treatments on physiological and metabolite accumulation of C. pilosula. The strains RHBA19 and RHBA17 were isolated and identified as Geobacillu sp. and Pseudomonas fluorescens, res...
Article
Full-text available
Natural hydrogels are growing in interest as a priority for wound healing. Plant polysaccharides have a variety of biological pharmacological activities, and chitosan hydrogels have proven strong antimicrobial effects, but hydrogels prepared with polysaccharides alone have certain deficiencies. Polysaccharides from flowers of Lonicera japonica Thun...
Article
Full-text available
In this study, the second-order model, Fick’s second law of diffusion, and the Peleg model were used to evaluate the extraction kinetic model of polysaccharide (CPP) from Codonopsis pilosula. The characteristic functional groups, surface structure, and physical and chemical properties of CPP were analyzed by multi-spectroscopic and microscopic tech...
Article
In this study, after optimizing the extraction process of CPP (Codonopsis pilosula polysaccharides), CPPM (CPP microcapsules) were prepared. Subsequently, the structural characteristics and physicochemical properties were studied. The results showed that CPPM is a hollow sac-like structure with rough folds and protuberances and comes in spherical o...
Article
Full-text available
The crude polysaccharide (CPNP) of Codonopsis pilosula was obtained by hot-water extraction technology. The extraction kinetic model established according to Fick’s first law of diffusion and related parameters of polysaccharide was studied. CPNP microcapsules were prepared by blending with sodium alginate, Ca2+ ions and crude CPNP. The quality con...
Article
Resuscitation‐promoting factors (Rpfs) belong to peptidoglycan hydrolases, which participate in recovery of dormant cells and promoting bacteria growth. In this study, the resuscitation promoting factor rpf2 gene of R. erythropolis KB1 was expressed in Escherichia coli and purified by Ni2+ affinity chromatography. The purified recombinant fusion pr...
Article
Codonopsis pilosula is a traditional Chinese herbal medicinal plant and contains various bioactive components, such as C. pilosula polysaccharides (CPPs) and lobetyolin (Lob). Hydrogen peroxide (H2O2) and nitric oxide (NO) are gaseous molecule and have been well known for their ability to relieve some adverse influences on plant from abiotic stress...
Article
The stiffness and gelation temperature of thermo-responsive chitosan (CS), glycerophosphate (GP) hydrogel is not enough for differentiation of BMSCs and injecting use in bone defect repair. To improve these weaknesses, a kind of injectable hydrogel (PMCG) was prepared with Chitosan (CS), glycerophosphate (GP), polygonatum polysaccharide modified mo...
Article
A petroleum hydrocarbon degrading bacterium Rhodococcus erythropolis KB1 was used to investigate the bioremediation efficiency of the petroleum contaminated desert steppe soils. The total petroleum hydrocarbons (TPHs) removal efficiency in the soils was analyzed with different treatments including natural degradation, bioaugmentation with R. erythr...
Preprint
Full-text available
In this study, the Cu ²⁺ (120 mg/L) and Cr ⁶⁺ (80 mg/L) removal rate of S. rhizophila JC1 reached at 79.9% and 89.3%, respectively. Scanning electron microscopy showed that Pb ²⁺ and Zn ²⁺ had no obvious effect on cell structure, but the cells became smaller and brighter under Cu ²⁺ stress, and many nanoparticles formed on the cell surface under Cr...
Article
Full-text available
In this study, the extraction conditions of Nostoc commune Vauch polysaccharide (NCVP) were optimized by single factor and orthogonal experiments. Then, the microcapsules (NCVPM) was prepared. After analyzing the microcapsule structural, thermal characteristics, the skin wound healing ability was studied by establishing back trauma rat models. Resu...
Article
Full-text available
As a basic technique of molecular cloning, bio-transformation has been successfully used in the fields of biomedicine and food processing. In this study, we established a transformation system of exogenous DNA into E. coli cells mediated by ultrasound. Under the optimal conditions (i.e. 35 °C, 40 W, 25 s, OD600 = 0.4–0.6) optimized by RSM, the tran...
Article
Full-text available
Aim of present study was to investigate the interaction of coomassie brilliant blue G-250 (CBBG-250) with bovine serum albumin (BSA) by the multispectroscopic methods. Fluorescence-data showed that the complex of BSA-CBBG-250 forming made the intrinsic fluorescence quenching of BSA by CBBG-250 interaction. BSA also could interact with CBBG-250 and...
Article
Full-text available
Biologically active coating materials could promote the growth of granulation tissue as auxiliary materials, while natural polysaccharides could promote vascular regeneration and wound healing. Therefore, in this study, UAP (ultrasound-assisted extraction of NCV polysaccharides) yield after the process optimization was 12.89 ± 0.24 %, which was use...
Article
Full-text available
Numerous bacteria entered the viable but non-culturable state due to the stresses of dry and salt in soils. YeaZ of Gram-negative bacteria is a resuscitation promoting factor (Rpf) homologous protein could resuscitate bacteria of natural environment in VBNC state. To investigate the promoting effect of YeaZ on the isolation of viable but non-cultur...
Article
Full-text available
Purpose To investigate the effect of Danggui Buxue Tang (DBT) on intestinal microbiota diversity after fermentation by Bacillus subtilis . Methods B. subtilis was used to ferment DBT. Sprague Dawley (SD) rats were randomly divided into the following four groups with six rats in each group: the control group, DBT nonfermentation group, B. subtilis...
Article
Full-text available
Background Pancreatic cancer (PC) has much weaker prognosis, which can be divided into diabetes and non-diabetes. PC patients with diabetes mellitus will have more opportunities for physical examination due to diabetes, while pancreatic cancer patients without diabetes tend to have higher risk. Identification of prognostic markers for diabetic and...
Article
Full-text available
Polyphenol oxidase is the popular enzyme involved in fruit-vegetable browning and melanin synthesis. In the present paper, L-3-phenyllactic acid, a natural bacteriostatic substance, was investigated as an inhibitor of polyphenol oxidase. The results were demonstrated that the residual enzyme activity of polyphenol oxidase decreased gradually with t...
Article
Four hundred fifty four pyrosequencing was used to analyze the microbial community and diversity of oily farmland soil from Fushun, Liaoning Province, China. The results showed that a total of 114 Phyla, 262 Classes, 430 Orders, 685 Families, 869 Genera, and 294 Species were detected and summarized in the soil microbe. The petroleum hydrocarbons si...
Article
Full-text available
Bacterial gene transformation used with Escherichia coli as a desired microorganism is one of the important techniques in genetic engineering. In this study, the preparation of E. coli DH5α competent cells treated with SrCl 2 and transformation by heat-shock with pUC19 plasmid was optimized by Response Surface Methodology (RSM). Other five E. coli...
Article
Foot-and-mouth disease virus (FMDV) nonstructural protein 3A plays important roles in virus replication, host range, and virulence. To further understand the role of 3A during FMDV infection, identification of host cell factors that interact with FMDV 3A is needed. Here, we found that vimentin is a direct binding partner of FMDV 3A, and manipulatio...
Article
Full-text available
The glycopeptidase GCP and its homologue proteins are conserved and essential for survival of bacteria. The ygjD gene (Glycopeptidase homologue) was cloned from Vibrio harveyi strain SF-1. The gene consisted of 1,017 bp, which encodes a 338 amino acid polypeptide. The nucleotide sequence similarity of the ygjD gene with that of V. harveyi FDAARGOS...
Article
The YeaZ protein of V. harveyi was expressed in Escherichia coli and purified. The purified recombinant protein YeaZ exhibited the protease activity. The proteolytic activities with azocasein as substrate were 39130 U mg‐1. The mutation of the amino acid in active sites such as Asp88, Ser185 and Trp169 were performed. The enzyme activities of the p...
Article
The extraction kinetics and thermodynamic parameters of Glycyrrhiza polysaccharide (GP) were studied, and the rheological properties and antiviral activity of GP were evaluated. The results showed that the extraction process was fitted with Fick's second law of diffusion. The optimum concentration (97.62 mg/mL) was obtained at solid-liquid ratio (1...
Article
The extraction process of Glycyrrhiza soluble polysaccharide (GP) was optimized by RSM, a rat trauma model was established via longitudinal incision on the back skin. The effects of GP combined with microcapsule collagen on the repair of rat injury model were discussed at different levels, Based on the content of hydroxyproline at the whole animal...
Article
Resuscitation-promoting factors (Rpf) are a class of muralytic enzymes, which participate in recovery of dormant cells and promoting bacteria growth in poor media. In the present study the expression vector of the rpf-1 gene from an oil-degrading bacterium Rhodococcus erythropolis KB1 was constructed and expressed in Escherichia coli. The expressed...
Article
Full-text available
Molecular studies of copper and cadmium resistances in acidophilic bacteria are significant in biomining. In this study, afe_1862, which encodes a heavy metal-binding protein in Acidithiobacillus ferrooxidans L1, was amplified using PCR, cloned into the pET32a plasmid, and sequenced. Following SDS-PAGE analysis, optimization of the expression condi...
Article
Based on the theory of extraction and diffusion of Chinese herbal medicine, the dynamic model of ultrasonic-assisted extraction process of polysaccharide from Nostoc commune Vauch. was established according to the second law of Fick, and further verified at different solid-liquid ratio (1/40–1/80 g/mL), temperature (313.15–353.15 K), ultrasonic pow...
Article
The yield, composition, structure and biofunction of microorganism exopolysaccharide (EPS) depended on their genetics and metabolic pathways. An EPS-producing endophytic fungus was isolated from Angelica sinensis planted in Dingxi, Gansu, China, and identified as Alternaria tenuissima F1. The production optimization, purification and characterizati...
Article
BACKGROUND In order to develop active packaging of lily products, we for the first time investigated the effects of phenyllatic acid (PLA) incorporated into a sodium alginate (SA)‐based coating on the quality of minimally processed lily bulbs stored at 4 °C for 15d. RESULTS L9 (3⁴) orthogonal array design was showed that the optimal concentrations...
Article
Glycyrrhiza uralensis is an important traditional Chinese herbal medicine. Polysaccharide is one of active components of G. uralensis. In this paper, the extraction conditions of crude polysaccharides from G. uralensis (GPs0) were optimized by central composite design (CCD), and Three purified polysaccharide fractions (GPs1, GPs2 and GPs3) were stu...
Article
Hydrogels have good water retention, biocompatibility and biodegradability, so they are well used in the medical industry. Here, we have active polysaccharide exacted from Chinese traditional medicine and carboxymethyl chitosan cross-linked to form hydrogel and characterized them by Scanning electron microscopy, FTIR analysis, swelling, degradation...
Article
The extraction process of crude polysaccharides from Glycyrrhiza (GP) by ultrasonic assisted hot water method was established according to the optimized kinetic model based on Fick's second law of diffusion, and thermodynamic action was analyzed. Physicochemical properties of GP including the apparent viscosity, thermostability and antioxidant acti...
Article
The extraction process, purification and characterization analyses of polysaccharides (LZJP) in Ziziphus jujuba planted in Linze County, Gansu Province were investigated, respectively. The results showed a maximum polysaccharide yield of 5.72% was achieved at a solid/liquid ratio of 1:20 g/mL for 90 min at 80 °C. Two homogenous acidic polysaccharid...
Article
The aim of this study was to investigate the effects of a low dietary protein and high carbohydrate infant formula on the large intestine of neonatal rats. A total of 24 neonatal SD rats (14d old) were randomly assigned to the low-protein, high-carbohydrate infant formula-fed group (IF group) and a human breast milk-fed group (HF group). After 7 da...
Article
In this study, the effects of ethanol fumigation on the prevention of enzymatic browning and microbial quality on fresh‐cut “Niu‐tui” Chinese yam were investigated. Yam discs were treated with ethanol (50, 100, and 200 µL/L) for 2 hr of fumigation, packaged in sealable storage bags, and depoted at 5 °C. Yam discs treated with ethanol fumigation sho...
Article
Scaffolds made from single hydroxyapatite possess neither biological properites of bone nor functions of promoting vascularization and inhibiting immune rejection. In order to overcome these drawbacks of hydroxyapatite (HAp), we prepared the ASP/HAp composite scaffolds and studied its application possibility in bone tissue engineering. The scaffold...
Article
Seasonal variations effects on desert steppe soil culturable bacteria in northwestern China and physicochemical parameters were investigated. Soil temperature ranged from 5.37 to 23.73 °C. Moisture content varied from 3.22% to 7.62%. The pH was between 7.98 and 8.72. Soil nutrients were altered in different seasons. Total potassium (K) remained the...
Article
The formation of thrombosis has limited the applications of small diameter vascular in cardiovascular diseases. In order to improve the anticoagulant activities of scaffolds, this study combined fucoidan with CS/PVA and investigated the complete physicochemical and mechanical characterization of the scaffolds to evaluate the feasibility of Fucoidan...
Article
Full-text available
Biological hydrogel is important in drug delivery system and tissue engineering. In this paper, we prepared a series of biological hydrogels with N,O-carboxymethyl chitosan and oxidized pumpkin polysaccharide-II (oxidized PP-II). Morphological analyses indicated that the N,O-carboxymethyl chitosan/oxidized PP-II hydrogels (CPHs) had porous interior...
Article
To obtain maximal linear regression correlation coefficient (R²) of glucose standard curve determinations, single-factor test and response surface methodology were applied to optimize conditions including reaction time, incubation temperature and phenol concentration. For investigating optimization of this method, the effects of different metal ion...
Article
Full-text available
Purpose: To develop a novel biotechnological method for removing toxic arsenic from two kinds of representative arsenic-containing ores using different mixed mesophilic acidophiles.Methods: Bioleaching of the two types of arsenic-containing ores by mixed arsenic-unadapted Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans or mixed ars...
Article
Piteguo fruit (PTG) picked from the trees growing at a high altitude of 2100-2400. m in Gansu Province, China, is rich in a variety of nutrients, especially the polysaccharide. The optimum conditions for ultrasonic-assisted extraction Piteguo polysaccharide (PTGP) were investigated by response surface methodology (RSM), including ratio of water to...
Article
Although distillers grains (DG) is an inexpensive and abundant co-product that contains valuable cellulose, it is currently not being used for industrial applications. In this study, microcrystalline cellulose (DGMCC) was successfully prepared from DG cellulose (DGC) using acid hydrolysis method. The effects of processing parameters like hydrochlor...
Article
To optimize ultrasound-assisted extraction technology of tannin from the succulent stem of Cynomorium songaricum. The content of tannin in C. songaricum samples of different source was comparatively studied. Extraction rate of tannin as indexes, extraction process was optimized by single factor experiment and L9(34) orthogonal experiment. The best...
Article
Extraction process was optimized by single factor and orthogonal experiment (L9(34)). Moreover, the content determination was studied in methodology. The optimum ultrasonic extraction conditions were: ethanol concentration of 75%, ultrasonic power of 420 w, the solid-liquid ratio of 1:15, extraction duration of 45min, extraction temperature of 90°C...
Article
Full-text available
Purpose: To optimize the extraction technology of polysaccharides from Cynomorium songaricum Rupr by ultrasonic-assisted extraction (UAE). Methods: Four parameters including ultrasonic power, ratio of raw material to water, extraction temperature, and extraction time were optimized by orthogonal design. The effects of the factors on the yield of po...
Article
Distillers grain (DG) is an abundant and low-cost lignocellulosic biomass. The objective of paper is to compare the influence of different acids on hydrolysis of DG and select the best acid. The results indicate that sulfuric acid is the most effective acid, and the optimum hydrolysis conditions are temperature 100°C, sulfuric acid concentration 2....
Article
The conventional extraction methods for flavonoid were time-consuming, laborious and energyconsuming. In this article, ultrasonic-assisted extraction (UAE) was utilized to extract flavonoid from Coreopsis tinctoria Nutt. The factors influencing the extraction efficiency were studied, including the effects of ultrasonic power, extraction time, a rat...
Article
Response surface methodology (RSM) was applied to predict optimum conditions for microwave-assisted extraction (MAE) of crude water-soluble polysaccharides from Piteguo fruit (PTG). A central composite design was used to optimize microwave power (X,: 400 - 600W), extraction time (X,: 5 - 10 min) and ratio of water to material (W/M ratio, X,: 30:1 -...
Article
The preparation of competent cell is the central step of bacteria transformation and has a great impact on transformation efficiency of nucleic acid. The aim of the research was to study the factors such as ionic species, strain types, plasmid concentration, ice-bath time and incubation time. The result shows that ionic species is fatal to the tran...
Article
Porous composite scaffold with Ligusticum wallichii polysaccharide and squid skin collagen was synthesized and characterized. The composite scaffold showed good phosphate buffer solution (PBS) retention ability, slow biodegradation rate, high mechanical strength, and superb cell proliferation promoting ability. The PBS content value increased from...
Article
Distiller's grains (DG) is an abundant source of biomass that can be hydrolyzed to yield fermentation sugar for the production of fuel ethanol. In order to improve the saccharification efficiency and investigate the hydrolysis characteristics of DG, this research studied the effect of temperature, mixed acid concentration, the solid-liquid ratio an...

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