Yoke Kqueen Cheah

Universiti Putra Malaysia | UPM · Department of Biomedical Sciences

Canine mammary gland tumour (CMT) commonly affects the female dog. The objective of this study was to develop a doxorubicin-resistant CMT cell line and determine its in vitro and in vivo characteristics, including mRNA and microRNA (miRNA) expression profiles. Doxorubicin-resistant CMT-Star cells were developed from CMT-Stylo cells. The cells were...

Gold-based anticancer compounds have been attracting increasing research interest due to their ability to kill cancer cells resistant to platinum-based compounds. Gold I- and gold III-based complexes have shown satisfactory anticancer activities. In this study, two new fluorine-incorporated gold (I) compounds such as Ph3PAu[SC(OMe)=NC6H4F-3] and DP...

Angelman syndrome (AS) is one of the common genetic disorders that could emerge either from a 15q11–q13 deletion or paternal uniparental disomy (UPD) or imprinting or UBE3A mutations. AS comes with various behavioral and phenotypic variability, but the acquisition of subjects for experiment and automating the landmarking process to characterize fac...

A genomic analysis study presents the whole-genome sequencing and annotation of Barrientosiimonas humi gen. nov., sp. nov. 39T to unravel the genomics data of B. humi sequences and understand the related biological functions information expression. Whole genome of B. humi isolate was sequenced using PacBio Sequel and then polished by Illumina HiSeq...

Thirteen out of the 15 known Cryobacterium spp. were from extremely cold environments. However, the fundamental question on their cold adaptation strategies to survive in the cold has not been addressed adequately. Hence, this work was conducted to determine the Cryobacterium sp. SO1 cold adaptation strategies. Cryobacterium sp. SO1 that grew optim...

Life Sciences, Medicine and Biomedicine was established in 2017 as an open access, multidisciplinary, peer-reviewed journal. The journal welcomes submissions in all areas of life sciences, medicine and biomedicine with a selection of relevant biological sciences on a case by case basis. It is expected that the wide scope of the journal will promote...

Life Sciences, Medicine and Biomedicine was established in 2017 as an open access, multidisciplinary, peer-reviewed journal. The journal welcomes submissions in all areas of life sciences, medicine and biomedicine with a selection of relevant biological sciences on a case by case basis. It is expected that the wide scope of the journal will promote...

Osteosarcoma (OS) is a life-threatening malignant bone tumor associated with poor prognosis among children. The survival rate of the patient is still arguably low even with intensive treatment provided, plus with the inherent side effects from the chemotherapy, which gives more unfavorable outcomes. Hence, the search for potent anti-osteosarcoma ag...

The dataset of Barrientosiimonas humi gen. nov., sp. nov. 39T from Antarctica https://www.ebi.ac.uk/ena/browser/view/PRJEB44986?show=publications

Background: Bacteria such as the Salmonella species had been extensively studied not only for its mechanism of pathogenicity, but for its beneficial utilisation in bacterial-mediated tumour therapy. Genetically modified bacterial strain, BDLA Salmonella Agona (BDLA S. Agona) had shown great promise as tumour targeting and suppressing agent. By, con...

Introduction: Bacteria had long been known to have tumour-targeting and tumour inhibition capabilities and have re-emerged into the limelight of cancer research as a possible alternative treatment for solid tumours. Conventional therapies for solid tumours are either by surgery, chemotherapy, radiotherapy, which are very invasive and non-specific t...

Background: Bacteria such as the Salmonella species had been extensively studied not only for its mechanism of pathogenicity, but for its beneficial utilisation in bacterial-mediated tumour therapy. Genetically modified bacterial strain, BDLA Salmonella Agona (BDLA S. Agona) had shown great promise as tumour targeting and suppressing agent. By, con...

Diet manipulation alters the gut microbiota composition. Gut dysbiosis is characterised by imbalanced bacteria composition that has been associated with high fat diet. Diets containing high animal fat induce pathogenic bacteria growth and similar bacterial profiles have been identified in obese adults and chronic disease patients. Conversely, diets...

Background Emergence of Candida glabrata , which causes potential life-threatening invasive candidiasis, has been widely associated with high morbidity and mortality. In order to cause disease in vivo, a robust and highly efficient metabolic adaptation is crucial for the survival of this fungal pathogen in human host. In fact, reprogramming of the...

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Breast cancer is one of the leading causes of cancer-related deaths in women worldwide, and its incidence is on the rise. A small fraction of cancer stem cells was identified within the tumour bulk, which are regarded as cancer-initiating cells, possess self-renewal and propagation potential, and a key driver for tumour heterogeneity and disease pr...

Hypoxia plays a significant role in solid tumors by the increased expression of hypoxia-inducible factor-1α (HIF-1α), which is known to promote cancer invasion and metastasis. Cancer-cell invasion dynamically begins with the degradation of the extracellular matrix (ECM) via invadopodia formation. The chemical substrates that are utilized by hypoxic...

Hypoxia plays a significant role in solid tumors by the increased expression of hypoxia-inducible factor-1α (HIF-1α), which is known to promote cancer invasion and metastasis. Cancer-cell invasion dynamically begins with the degradation of the extracellular matrix (ECM) via invadopodia formation. The chemical substrates that are utilized by hypoxic...

Down syndrome (DS), is the most common cause of intellectual disability, and is characterized by defective neurogenesis during perinatal development. To identify metabolic aberrations in early neurogenesis, we profiled neurospheres derived from the embryonic brain of Ts1Cje, a mouse model of Down syndrome. High-throughput phenotypic microarray reve...

One of the most pertinent applications of image analysis is face recognition and one of the most common genetic disorders is Down syndrome (DS), which is caused by chromosome abnormalities in humans. It is currently a challenge in computer vision in the domain of DS face recognition to build an automated system that equals the human ability to reco...

MicroRNAs (miRNAs) are small non‐coding RNAs that can post‐transcriptionally regulate the genes involved in critical cellular processes. The aberrant expressions of oncogenic or tumor suppressor miRNAs have been associated with cancer progression and malignancies. This resulted in the dysregulation of signaling pathways involved in cell proliferati...

Thermophilic microorganisms have always been an important part of the ecosystem, particularly in a hot environment, as they play a key role in nutrient recycling at high temperatures where most microorganisms cannot cope. While most of the thermophiles are archaea, thermophiles can also be found among some species of bacteria. These bacteria are ve...

Feline infectious peritonitis (FIP) is a fatal feline immune-mediated disease caused by feline infectious peritonitis virus (FIPV). Little is known about the biological pathways associated in FIP pathogenesis. This is the first study aiming to determine the phenotypic characteristics on the cellular level in relation to specific metabolic pathways...

Background: Landmark-based approaches of two- or three-dimensional coordinates are the most widely used in geometric morphometrics (GM). As human face hosts the organs that act as the central interface for identification, more landmarks are needed to characterize biological shape variation. Because the use of few anatomical landmarks may not be su...

Background The application of three-dimensional scan models offers a useful resource for studying craniofacial variation. The complex mathematical analysis for facial point acquisition in three-dimensional models has made many craniofacial assessments laborious. Method This study investigates three-dimensional (3D) soft-tissue craniofacial variati...

Sexual dimorphism in Homo-sapiens is a phenomenon of a direct product of evolution by natural selection where evolutionary forces acted separately on the sexes which brought about the differences in appearance between male and female such as in shape and size. Advances in morphometrics have skyrocketed the rate of research on sex differences in hum...

Over the years, neuroscientists and psychophysicists have been asking whether data acquisition for facial analysis should be performed holistically or with local feature analysis. This has led to various advanced methods of face recognition being proposed, and especially techniques using facial landmarks. The current facial landmark methods in 3D i...

Background: Expression in H-sapiens plays a remarkable role when it comes to social communication. The identification of this expression by human beings is relatively easy and accurate. However, achieving the same result in 3D by machine remains a challenge in computer vision. This is due to the current challenges facing facial data acquisition in...

Cloning of Candida glabrata ITS 1/2 gene for standardization of Loop-mediated isothermal amplification

World Health Organization (WHO) reports antibiotic resistance is one of the biggest threat to global health, food security, modern medicine and development today. Antibiotic resistance can affect anyone, of any age, in any country. Some bacteria pathogens are resistant to multiple antibiotics, they are multidrug-resistant, which facilitates the spr...

Barrientosiimonas humi is a novel soil actinobacterium isolated from the Barrientos Island in Antartica. The taxonomic status of this bacterium was previously determined through the analysis of the 16S rRNA gene sequence and the 39T strain of this bacterium was proven to have a novel lineage within the family of Dermacoccaceae and was most closely...

Cancer metastasis is a multistep process, which results in cancer cells disseminating to other organs. The crucial metastasis step involves cancer invasion which occurs via actin-protrusion by invasive malignant cells, termed as invadopodia. In solid tumours, invadopodia formation increases as a result of hypoxia which is found to be resistant agai...

Flexibility in carbon metabolism is pivotal for the survival and propagation of many human fungal pathogens within host niches. Indeed, flexible carbon assimilation enhances pathogenicity and affects the immunogenicity of Candida albicans. Over the last decade, Candida glabrata has emerged as one of the most common and problematic causes of invasiv...

The purpose of this study is to characterize 3 non-albicans Candida spp. that were collected from two major hospitals in a densely populated area of Kuala Lumpur for their susceptibilities to azole and genetic background. Fifteen non-albicans Candida clinical isolates in two major hospitals in Kuala Lumpur area of Malaysia were collected by conveni...

The human fungal pathogen Candida glabrata appears to utilise unique stealth, evasion and persistence strategies in subverting the onslaught of host immune response during systemic infection. However, macrophages actively deprive the intracellular fungal pathogen of glucose, and therefore alternative carbon sources probably support the growth and s...

Cellular components of the tumour microenvironment (TME) are recognized to regulate the hallmarks of cancers including tumour proliferation, angiogenesis, invasion, and metastasis, as well as chemotherapeutic resistance. The linkage between miRNA, TME, and the development of the hallmarks of cancer makes miRNA-mediated regulation of TME a potential...

Isothermal amplification is a technique that can amplify target DNA sequences at a single incubation temperature. Loop-mediated isothermal amplification (LAMP) is an extension of the isothermal DNA amplification technique that combines rapidity, simplicity, high specificity and sensitivity. Due to its overwhelming characteristics, LAMP has been exp...

The fight against cancer has been a never-ending battle. Limitations of conventional therapies include lack of selectivity, poor penetration and highly toxic to the host. Using genetically modified bacteria as a tumour therapy agent has gained the interest of scientist from the past few decades. Low virulence and highly tolerability of Salmonella s...

Torque teno virus (TTV) is one of the “orphan” viruses that have been discovered almost two decades ago, with little information on the relationship of the infection to any diseases. It is one of the 45% of the commensal virus which is found throughout the population and becoming one of the most extensively studied viruses on its prevalence among t...

Global warming is the main concern in today’s century as it comes with numerous side effects on the natural environment. Open Top Chambers (OTC) consist of metal constructions with transparent vertical side-walls and a frustum on top. An opening in the middle of the frustum allows an air exchange to reduce temperature and humidity effects in the ch...

Background and Objectives: Medicinal plants play a vital role in the culture of healing in the various ethnic in Malaysia. All of the ethnic have shared or unique methods of using the medicinal plants available in Malaysia. However, the looming crisis of deforestation and urbanization, the medicinal plants are in danger of extinction. Therefore, th...

High invasive cancer cells are thought to recruit specialised actin-rich protrusions for invasion in metastasis process. These protrusions are termed invadopodia. To study invadopodia formation, one of the first challenges faced by researchers has been to optimise the cell line passage number in order to be used for the invadopodia assay. Therefore...

A new dipeptide, (-)-glaciantarcin (1) and three known compounds, cyclo(-Pro-Gly) (2), 1-(2-deoxypentofuranosyl)-5-methyl-2,4(1H,3H)-pyrimidinedione (3) and vidarabine (Ara-A) (4), were isolated from Glaciozyma antarctica PI12, a cold-adapted yeast. The chemical structures were elucidated by FT-IR, NMR and mass spectrometry. The cytotoxicity and an...

King George Island (KGI) and Deception Island (DCI) are members of the South Shetland Islands in Antarctica, each with their own landscape and local environmental factors. Both sites are suitable for long-term monitoring of bacterial diversity shift due to warming, as temperature rises relatively faster than East Antarctica. This study was conducte...

Psychrophilic and psychrotrophic bacteria play important roles in nutrient cycling in cold environments. These bacteria are suitable as model organisms for studying cold-adaptation, and sources of cold-active enzymes and metabolites for industrial applications. Here, we report the genome sequences of two Cryobacterium sp. strains SO1 and SO2. Genes...

Red palm oil (RPO) is a natural source of Vitamin E (70-80% tocotrienol). It is a potent natural antioxidant that can be used in skin-care products. Its antioxidant property protects skin from inflammation and aging. In our work, a tocotrienol-rich RPO-based nanoemulsion formulation was optimized using response surface methodology (RSM) and formula...

Red palm oil (RPO) is a natural source of Vitamin E (70–80% tocotrienol). It is a potent natural antioxidant that can be used in skin-care products. Its antioxidant property protects skin from inflammation and aging. In our work, a tocotrienol-rich RPO-based nanoemulsion formulation was optimized using response surface methodology (RSM) and formula...

Readings of carotenoids and vitamin E content in Table 2. (DOCX)

Readings of droplet size of nanoemulsion in Table 3. (DOCX)

Readings of droplet size of nanoemulsion in Fig 2. (DOCX)

Readings of droplet size of nanoemulsion in Fig 1. (DOCX)

Readings of droplet size and PDI of nanoemulsion in Table 6. (DOCX)

The trend of decrease in droplet size when increase in concentration of surfactant and glycerol was also found when homogenization pressure was at 500 and 700. Response surface plot showing the effect of 10 wt% of surfactant concentration, 20 wt% of glycerol concentration and (A) 500 bar homogenization pressure and (B) 700 bar homogenization pressu...

Kitchen mishandling practices contribute to a large number of foodborne illnesses. In this study, the transfer and cross-contamination potential of Vibrio parahaemolyticus from bloody clams to ready-to-eat food (lettuce) was assessed. Three scenarios were investigated: 1) direct cross-contamination, the transfer of V. parahaemolyticus from bloody c...

Microbes play a very important role in each individual. The microbial communities and its genetic blueprint greatly influence in many human diseases. Most of the microbe populations are grow in an individual’s gut. Therefore, metagenomics studies on gut microbes are essential to understand the microbial diversity in gut and the knowledge on micro...

Annona muricata or soursop belongs to the family Annonaceae and is a plant enriched with annonaceous acetogenin. This plant had been traditionally used for the treatment of various infectious and inflammatory diseases. This study aimed to determine the cytotoxicity, antitumor-promoting and antioxidant activities of Annona muricata various extracts...

Methicillin-resistant Staphylococcus aureus (MRSA) is a remarkable pathogen which causes nosocomial diseases worldwide. In this study, raw green mussel and cockle samples in Malaysia were collected and investigated for the occurrence of MRSA. A total of 80 samples were analyzed using phenotypic and molecular approaches. A assembling of the most pro...

Bacterial endophytes are found on all types of plants and is a potential source of bioactive compounds which can be utilized to fight against multi-resistant pathogens and could be further develop into new leads for antibiotic development. However, the research done on the bacterial endophytes is relatively new and has potential to grow as it is th...

Osteosarcoma is one of the primary malignant bone tumors that confer low survival rates for patients even with intensive regime treatments. Therefore, discovery of novel anti-osteosarcoma drugs derived from natural products that are not harmful to the normal cells remains crucial. Curcumin is one of the natural substances that have been extensively...

Osteosarcoma is one of the primary malignant bone tumors that confer low survival rates for patients even with intensive regime treatments. Therefore, discovery of novel anti-osteosarcoma drugs derived from natural products that are not harmful to the normal cells remains crucial. Curcumin is one of the natural substances that have been extensively...

Osteosarcoma is one of the primary malignant bone tumors that confer low survival rates for patients even with intensive regime treatments. Therefore, discovery of novel anti-osteosarcoma drugs derived from natural products that are not harmful to the normal cells remains crucial. Curcumin is one of the natural substances that have been extensively...