Yibing Liu

• PhD
• Professor (Associate) at Ocean University of China

Chicoreus torrefactus, which has a typical crossed lamellar structure, was utilized as an ideal species to investigate the structure and function of the mantle in shell formation. Field emission scanning electron microscopy was used to study the microstructure of the gastropod shell. Histological methods, histochemical methods and transcriptome seq...

Previous studies have shown that post-thaw sperm performance is affected by multiple stressors during cryopreservation, such as those induced by physical, chemical, mechanical and physiological changes. One of these is the balance disturbance between the antioxidant defense system and reactive oxygen species (ROS) production. This study investigate...

The germplasm of farmed Yesso scallop Patinopecten yessoensis in China has deteriorated since its introduction more than 40 years ago. The main aim of this study is to develop a sperm cryopreservation technique to assist the newly established program to manage this issue in China. This study investigated the factors important to the development of...

This study examines the global waste plastic trade, impacted by China's import ban and the COVID-19 pandemic, using post-pandemic data to analyse trade flows and project future volumes via ridge regression. From 1988 to 2022, global waste plastic exports totaled approximately 255 million tons, with major contributions from high-income regions like...

Sperm cryopreservation technique has been published in many farmed bivalve species. One of the key factors preventing its application in aquaculture and/or cryobanking is the knowledge gap on the performance of resultant progeny at late developmental stages and subsequent generations. An effective strategy to overcome these challenges is to use a m...

High mortalities and highly variable results during the subsequent development of post-thaw larvae have been widely considered as key issues restricting the application of cryopreservation techniques to support genetic improvement programs and hatchery production in farmed marine bivalve species. To date, few studies have been undertaken to investi...

Cryopreservation is a technique to maintain biological materials’ physiological and genetic stability at an ultralow temperature. For commercially important livestock or aquatic species, gamete and embryo cryopreservation could play a significant role in breeding programs and commercial production. For example, it could help overcome key problems s...

This study evaluated the effects of larval cryopreservation on redox status of post-thaw trochophore larvae and on gene expression in post-thaw trochophore larvae, D-larvae, umbo larvae, eyed larvae and spat, with the aim to understand the effects of cryodamage in the Pacific oyster Crassostrea gigas. The results showed that total antioxidant capac...

The relative mortality rate of trochophore larvae exposed to cryopreservation is significantly higher at early larval developmental stages in blue mussels Mytilus galloprovincialis. However, little is known about cryodamage on the larval quality at cellular and molecular modifications in aquatic species, including M. galloprovincialis. This study a...

This study investigated three vital factors to develop a programmable cryopreservation technique for the larvae of Pacific oyster Crassostrea gigas, namely (1) larval developmental stages; (2) cryoprotectant agents (CPAs) and (3) larval density. The D-larval rate was used as the post-thaw survival indicator, which was calculated as the percentage o...

This study investigated the factors important to the development of a programmable larval cryopreservation technique in Mytilus galloprovinvialis, including (1) larval developmental stages; (2) cryoprotectant agents (CPAs); (3) thawing temperatures; (4) sucrose concentrations to remove CPA after thawing and (5) straw volumes, using D-larval rate as...

This study investigated the effects of starvation and refeeding on the histological features of stomach and intestine in Penaeus japonicus Spence Bate, 1888. Five treatment groups of P. japonicus were initially starved for 0, 5, 10, 15 and 25 days (designated as S 0 , S 5 , S 10 , S 15 and S 25 , respectively) and subsequently fed for 15 days. The...

In this study, a programmable freezing technique has been developed for strip spawned sperm in the blue mussel, Mytilus galloprovincialis. The optimized key parameters include cooling rate, endpoint temperature, thawing temperature, sugar addition and sperm to oocyte ratio. The sperm quality was assessed by the fertilization rate or the integrity o...

Mass mortality in the early stage of fish larvae frequently occurs in marine fish hatchery, and low survival has become a major bottleneck hindering fingerling production of marine fish. Inappropriate food supply is a key factor leading to fish mortality during the nursery period in a hatchery. The success of fish larvae to capture live prey in ear...

Cryopreservation offers long-term storage of gametes without constraint from seasonal gamete maturation, provides opportunities to improve the efficiency of breeding and genetic programs, and protects endangered species from extinction due to epidemic diseases and natural disasters. In this study, a protocol for cryopreserving sperm of the spermcas...

This study investigated the effects of broodstock age (2- and 3-yr-old), and sperm collection time at the beginning, middle, and end of a natural spawning period, on the ability of sperm to tolerate cryopreservation in farmed greenlip abalone, Haliotis laevigata. The quality of sperm was assessed by motility, fertilization rate, plasma membrane int...

In this study, a non-programmable freezing technique has been developed with the strip spawned blue mussel (Mytilus galloprovincialis) sperm. The key parameters optimized including (1) cryoprotectant agents (CPAs); (2) cooling temperature; (3) thawing temperature; (4) sugar and amino acid supplementation and (5) sperm to egg ratio. The fertilizatio...

Sperm cryopreservation is a valuable technique that can enhance the efficiency of genetic improvement programs, commercial production and endangered species conservation. Although studies on sperm cryopreservation in marine mollusks started late in comparison with livestock and finfish, research has substantially increased for the major economical...

This study investigated factors key to the development of sperm cryopreservation in the greenlip abalone Haliotis laevigata using a programmable freezing technique, including (1) permeable cryoprotectant agent (CPA) selection; (2) cooling rate; (3) endpoint temperature; (4) thawing temperature; (5) sperm to egg ratio and (6) sugar, vitamin and amin...

Greenlip abalone (Haliotis laevigata) is one of the most economically valuable marine molluscan species in Australia and its aquaculture production comprises more than 50% of the total production of farmed abalone in this country. The development of a cryopreservation technique for this species will in particular enhance the efficiency of the estab...

This study developed a technique of sperm cryopreservation using liquid nitrogen (LN) vapour in farmed blacklip abalone Haliotis rubra through evaluating the following five key factors: (1) cryoprotectant agent (CPA) toxicity; (2) cooling temperature; (3) thawing temperature; (4) sperm to egg ratio and (5) sugar addition, using sperm motility or fe...

This study investigated factorsimportant to the development of the liquid nitrogen (LN) vapour sperm cryopreservation technique infarmed greenlip abaloneHaliotis laevigata, including (1) cryoprotectant agent(CPA) toxicity; (2) cooling temperature (height above LN surface); (3) thawing temperature; (4) sperm to egg ratio; and (5) sugar supplementati...