
Yesenia Pacheco-HernándezCenter for Research and Advanced Studies of the National Polytechnic Institute | Cinvestav · Departamento de Ingeniería Genética (Irapuato)
Yesenia Pacheco-Hernández
PhD in Biotechnology Sciences
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42
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Publications
Publications (42)
The chemical variation of the annual essential oils from Pimenta dioca (PdEOs) grown in the Northern Highlands of Puebla, Mexico, and their biological activities were determined. Eugenol (>50%) and beta-myrcene (>16%) were the main volatiles contained in the annual PdEOs (2022-2024) extracted from fruit and leaves. PdEOs showed inhibitory propertie...
A therapeutic strategy to treat Type 2 diabetes mellitus (T2D) is focused on controlling postprandial glucose levels by inhibiting α-amylase and α-glucosidase. These enzymes are essential to convert assimilable carbohydrates into glucose. Nutraceuticals contained in plant foods exert inhibitory properties on these enzymes, which are comparable to s...
The Mexican tropical porcupine (Coendou mexicanus) is a threatened species in Mexico, according to the NOM-059 with habitat fragmentation being a primary cause. In the Sierra Norte de Puebla, a biogeographically diverse region, vegetation conducive to its presence is preserved. This article details the distribution of sightings in the area and incl...
The flowers of Yucca aloifolia (“flor de izote”) are considered a millenary food in the Northeastern Highlands of Puebla, Mexico. The present investigation reports on the chemical and biological activities of the hydroalcoholic extract (YAHF) obtained from this edible source. HPLC-MS profiling revealed twenty bioactive phenolic compounds with chlor...
Los quelites de México y otros alimentos nativos de origen vegetal representan una fuente de moléculas bioactivas cuyo consumo genera beneficios para la salud de la población. En esta revisión de carácter divulgativa se describen a los principales compuestos con actividad nutracéutica encontrados en plantas comestibles consumidas dentro del territo...
The 1H NMR metabolomics profiling of six edible mushrooms consumed in the northeastern highlands of Puebla, Mexico is presented. These fungi were morpho- and molecularly identified as Infundibulicybe squamulosa, Amanita jacksonii, Lepista nuda, Russula delica, Russula brevipes, and Lactarius indigo. The chemical profiling confirmed the presence of...
Herein, we present an integrative investigation on the nutritional and nutraceutical potential of Lactarius indigo, Clitocybe nuda, Clitocybe subclavipes, Russula delica, Russula brevipes, Clitocybe squamulosa, and Amanita jacksonii, which are edible mushrooms consumed in the Northeastern Highlands of Puebla, Mexico. The content of protein oscillat...
Herein we describe the in situ inhibitory activity of three hybrid films (FL1, FL2, and FL3) against two wild strains of Colletotrichum gloeosporioides and Penicillium digitatum as causal agents of rot in Persian limes. The films FL2 and FL3 contained 1.0 and 1.3 % weight/volume Litsea glaucescens essential oil (LgEO) and significantly (p < 0.05) d...
We present the inhibitory properties of the R. pompana anthocyanin fraction (RPAF) and its major constituents on alpha-glucosidase (AG), pancreatic lipase (PL), HMG-CoA reductase, and ornithine decarboxylase (ODC). The effect of RPAF was also evaluated in ICR male mice subjected to oral glucose tolerance test (OGTT) and hypercaloric/atherogenic die...
Resveratrol (RV), carnosic acid (CA) and hernandulcin (HE; a non-caloric sweetener) are envisioned as promising nutraceuticals to design new functional foods for improving lipid and carbohydrate metabolism. This study aimed to investigate the in vitro inhibitory effect of these molecules on specific enzyme targets and their capacity to improve dist...
Lepidopterism caused by caterpillar contact is considered a public health problem around the world. The local and systemic responses of this pathology include short-and long-term inflam-matory events. Although the proteolytic activity of the venoms from caterpillars is strongly associated with an inflammatory response in humans and murine models, f...
The northern highlands of Puebla harbor several plant foods consumed since pre-Columbian times. Most of the native vegetables from this geographical region are still uncharacterized at the nutraceutical level. This investigation was focused on the elucidation of chemical and biochemical properties of the edible organs from Rhamnus pompana, Solanum...
Celery is a vegetable widely consumed as a condiment to prepare diverse dishes around the world. Nevertheless, this plant is susceptible to the attack of several phytopathogens including those of the Fusarium genus which is translated into devastating losses for the production chain. Herein we report on the metabolic changes produced during the cel...
Hass avocadoes are one of the most popular fruits consumed worldwide because of their nutritional and nutraceutical content. Nevertheless, these fruits are susceptible to phytopathogen attacks that decrease fruit quality during the postharvest period. Herein we present the results of the in situ fungistatic activity of four hybrid films (FT1–FT4) m...
The northern highlands of Puebla are considered a new potential area for the cultivation of Hass avocado. Then, the emergence of new phytopathogens related to this geographical area is latent. The aim of this investigation was to isolate the microorganisms linked to the avocado fruit rot recently recorded by the producers of this locality in 2021....
p>The northern highlands of Puebla are considered a new potential area for the cultivation of Hass avocado. Then, the emergence of new phytopathogens related to this geographical area is latent. The aim of this investigation was to isolate the microorganisms linked to the avocado fruit rot recently recorded by the producers of this locality in 2021...
Brickellia veronicifolia is a native Asteraceae from Mexico that persists in fragmented habitats. This investigation reports the genetic and chemical diversity of B. veronicifolia. The diversity analysis based on iPBS markers showed an averaged Shannon index (S) of 0.3493, a Nei genetic diversity (h) of 0.2256, and a percentage of polymorphic loci...
Purpose: To determine the nutraceutical effect of mixtures containing Lactobacillus nagelii/betanin on the carbohydrate and lipid metabolism of mice. Methods: Lactobacillus nagelii and betanin were isolated from the raw materials. These components were subsequently assessed for their anti-hyperglycemic and hypolipidemic activities, and administered...
Introduction:
Nicotiana tabacum is a plant model intensively used in the bio-engineering pharmaceutical industry as a platform to produce drugs and therapeutic agents. Currently, no information regarding the non-targeted metabolome of transgenic tobacco containing recombinant regulatory sequences is available.
Objective:
To compare the proton nu...
Zanthoxylum limoncello is a native plant from Mexico used as a timber source, condiment and as a traditional medicine. The annual essential oils contained 15 organic compounds with a growth inhibitory activity against H. pylori ATCC 53504, 26695 and J99. The 2‐undecanone (31.6‐36.8%) and 2‐undecenal (25.1‐35.7%) were involved in the anti‐H. pylori...
The edible leaves of Peperomia maculosa were chemically investigated. Lutein (9.05–16.82 mg 100 g–1 FW), rutin (1.63–3.72 mg 100 g–1 DW), quercetin (2.04–5.75 mg 100 g–1 DW), and ascorbic acid (18.33–37.09 mg 100 g–1 DW) were quantified by HPLC. GC‐MS analyses of the leaf essential oil showed decanal (26.8%–47.9%) and lauric acid (22.6%–37.1%) as t...
The chemical composition of the seasonal essential oils (2015-2016) from the leaves and flowers of Zaluzania montagnifolia is presented. The chemical content of those oils showed quantitative and qualitative differences. Germacrene D (19.9-29.8%), camphor (12.4-19.4%) and β-caryophyllene (13.7-18.5%) were the most abundant volatiles in the leaves....
This study reports on the volatile profile and pharmacological activities of the essential oil and resin from Bursera slechtendalii, an aromatic medicinal tree commercialized in southern Mexico. The essential oil contained 25 volatiles including p-cymen-8-ol (25.4–35.7%), n-hexyl hexanoate (6.8–17.6%) and n-octyl hexanoate (7.6–14.8%), whereas the...
The present work describes the chemical composition of the leaf essential oils from the annual plant Dalea foliolosa, which was collected in three consecutive years (2014-2016). The amount of monoterpenes (69.5-84.6%) was higher to that of sesquiterpenes (10.3-22.9%) and aliphatic hydrocarbons (1-2.1%). Cryptone (22.3-30.6%) was the most abundant o...
The present work describes the chemical composition of the leaf essential oils from the annual plant Dalea foliolosa, which was collected in three consecutive years (2014-2016). The amount of monoterpenes (69.5-84.6%) was superior to that of sesquiterpenes (10.3-22.9%) and aliphatic hydrocarbons (1-2.1%). Cryptone (22.3-30.6%) was the most abundant...
The chemical composition of the essential oil and carotenoid content of the parasitic plant Cuscuta itraeformis are described for the first time. The essential oil was analyzed by GC-FID and GC-MS revealing nonanal (24.6%) as the main constituent followed by thymol (16.5%) and eugenol (7.5%). The total carotenoid content (130 mg 100 g-1 FW) was det...
We report the bifunctional activity of the native ent-kaurene oxidase from Montanoa tomentosa (MtKO) and its N-terminal modified version (LMtKO) for producing both isokaurenoic acid and kaurenoic acid in Saccharomyces cerevisiae. The Km app of MtKO showed more affinity for ent-kaurene (80.5 µM) than for isokaurene (96.4 µM). Interestingly, LMtKO ex...
Begonia nelumbiifolia is a traditional edible plant consumed and commercialized in the northern highlands of Puebla, Mexico. The present study reports the seasonal variation in proximate analysis as well as organic acids, carotenoids and flavonoids content in both leaves and stalks of this plant. The stalks contained low concentrations of protein (...
Beyond its vital role in plant metabolism, kaurenoic acid (KA) has acquired remarkable importance in the fields of medicinal chemistry and pharmacology. Here, we show and discuss the most recent scientific literature on the extraordinary versatility of KA, which is reflected in its hypoglycemic, analgesic, and selective cytotoxic properties. The sc...
Clinopodium macrostemum var. laevigatum is a Mexican medicinal plant appreciated because of the pleasant smell of its leaves. This work describes the seasonal volatile content of the leaves by SPME technique as well as the GC-MS analyses of four seasonal essential oils. Menthone (~34%) and piperitone oxide (~30%) were the most abundant compounds in...
Cytochrome P450 enzymes are important for biotechnology due to their capacity to modify diverse secondary metabolites that may produce chemicals with pharmacological properties. Most terpenes, flavonoids and alkaloids require P450 catalytic functions to reach their biological activity. In the last ten years, several efforts have focused on the expr...
Kaurene oxidases are P450 proteins that catalyze the conversion of
ent-kaurene into kaurenoic acid, the final enzymatic product with a wide
range of pharmacological properties. We describe the functional
characterization of an ent-kaurene oxidase (EC 1.14.13.78) isolated from
Montanoa tomentosa after heterologous expression in Saccharomyces
cerevis...
We report the results of in vitro anti-lipase and antioxidant assays using crude ethanolic extracts from 30 plants grown in Oaxaca, México. Anti-lipase tests were performed by using porcine pancreatic lipase (PPL) [EC 3.1.1.3] from Affymetrix/USB. The extracts of Solanum erianthum, Salvia microphylla, Brungmansia suaveolens and Cuphea aequipetala s...
Zaluzania montagnifolia, an antidiabetic and abortive shrub 1 to 3 m high, grows actively in xeric scrublands in some states of Mexico. Zulazanins and some flavonoids are the foremost constituents of the polar extracts in this species; however, there is no available information about the lipophilic compounds from non-polar extracts. The objective o...
Zaluzania montagnifolia, an antidiabetic and abortive shrub 1 to 3 m high, grows actively in xeric scrublands in some states of México. Zulazanins and some flavonoids are the foremost constituents of the polar extracts in this species; however, there is no available information about the lipophilic compounds from non-polar extracts. The objective o...
Herein we describe some aspects of the ethnobotanical use and the first alkaloid profile of Lupinus jaimehintoniana, the 5 to 8 m high arboreous lupine. Five quinolizidine alkaloids identified as sparteine, 5,6-dehydrolupanine, lupanine, nuttalline, and d-thermopsine, were characterized by the respective elution order according to their electronic...