yeong-suk Kim

Kookmin University · Department of Forest Products and Biotechnology

This study aims to visually inspect the effects of oxidized starch (OS) adhesives prepared with two cross-linkers namely citric acid (CA) and blocked-polymeric MDI (B-pMDI) on the extent of surface-inhabiting molds growth on the surface of medium-density fibreboard (MDF). Five surface-inhabiting molds namely Aspergillus niger (ASN), Penicillium f...

There are differences in the extracellular enzymes produced from species of wood-rotting fungi and their activity due to variation in nutritional conditions such as carbon sources and nitrogen sources, as well as environmental conditions such as incubation temperature and pH. In this study, to determine the methods to promote the secretion of espec...

This study was conducted on crude enzyme from the novel strain Acanthophysium sp. KMF001 using a mediator (surfactant). The surfactant was applied to the steam-exploded pretreated domestic wood biomass, and response surface methodology (RSM) was conducted to determine the optimum conditions for saccharification using the optimum substrate concentra...

This study investigated to understand the trend of international commercializing technologies and industrial status of the transportation biofuel based on lignocellulosic biomass. Two major commercializing technologies for the lignocellulosic biofuel are biochemical conversion technology and thermochemical conversion technology. It was reported tha...

This was investigated on the major issues and research trends regarding the heat-treatment of woods through literature reviews. The principal heat-treatment technologies utilized for industrial purposes include the Plato-process (Netherlands), the Retification process (France), the OHT-process (Germany), and the Thermowood Process (Finland). Factor...

Biopolishing using cellulases was introduced in the production of cotton fabric in order to improve the quality of fabric environmental friendly and is commonly used in the textile industry. In this study, the application of a crude cellulase from Acanthophysium sp. KMF001, which was excellent for the saccharification of cellulose, on biopolishing...

Elizabethkingia sp. BM10 was isolated from the hindgut of the wood-feeding termite Reticulitermes speratus KMT1. It had cellobiohydrolase and β-glucosidase activities but not endo-β-glucanase activity. The complete sequence of its genome, which has a total size of 4,242,519 bases, is reported here. The genomic analysis identified six β-glucosidase...

Sanitizing effect of ethanol on a Yersinia enterocolitica biofilm was evaluated in terms of biomass removal and bactericidal activity. We found that 40 % ethanol was most effective for biofilm biomass removal; however, no significant difference was observed in bactericidal activity between treatment with 40 and 70 % ethanol. This unexpected low eth...

The economical production of biofuels is hindered by the recalcitrance of lignocellulose to processing, causing high consumption of processing enzymes and impeding hydrolysis of pretreated lignocellulosic biomass. We determined the major rate-limiting factor in the hydrolysis of popping pre-treated rice straw (PPRS) by examining cellulase adsorptio...

Termites are wood pests that cause vast economic damage every year. They digest both cellulose and starch, but the enzymes for starch digestion have not been well characterized. We obtained complete amino acid sequence information on the KME1 -amylase from Reticulitermes speratus KMT1 through analysis of total mRNA sequences. The KME1 enzyme has tw...

Current research trends of non-copper wood preservatives for enhancing durability was reviewed; as a follow-up of the review in 2012 on copper-based wood preservatives. Main environmental friendly non-copper wood preservatives studied by many scientists were boron-based compounds, synthetic compounds from natural products, and pyrethroids family of...

Current research trends in wood preservatives for enhancing durability was reviewed. Due to leaching of recent Copper-Based Preservatives commonly used as chemicals for pressure treatment; they have been a growing concern, especially in improving the fixation of the copper as alkyl ammonium quat. and azol in wood and preventing the leaching of acti...

This study reviewed on the research trend of sources and utilization of the byproducts(Lignin) from bioethanol production process with lignocellulosic biomass such as wood, agri-processing by-products(corn fiber, sugarcane bagasse etc.) and energy crops(switch grass, poplar, Miscanthus etc.). During biochemical conversion process, only Cellulose an...

A screening for cellobiohydrolase (CBH) activity was performed and Fomitopsis pinicola KMJ812 was selected for further characterization as it produced a high level of CBH activity. An extracellular CBH was purified to homogeneity by sequential chromatography of F. pinicola culture supernatants. The molecular mass of the F. pinicola CBH was determin...

The optimum culture condition of Schizophyllum commune for the cellulase production and its enzymatic characteristics for saccharification of cellulosic biomass were analyzed. S. commune secrets {\beta}-1,4-xylosidase (BXL) and cellulases, including endo-{\beta}-1,4-glucanase (EG), cellobiohydrolase (CBH), and {\beta}-glucosidase (BGL). The optimum...

An extracellular xylanase was purified to homogeneity by sequential chromatography of Fomitopsis pinicola culture supernatants on a DEAE-sepharose column, a gel filtration column, and then on a MonoQ column with fast protein liquid chromatography. The relative molecular weight of F. pinicola xylanase was determined to be 58 kDa by sodium dodecylsul...

In this study, effect of carbon source on the hydrolytic ability of the enzyme from Fomitopsis pinicola, a brown rot fungi, for lignocellulosic biomass were examined on two lignocellulosic biomasses (rice straw and wood) without any pretreatment. Cellulase activities of crude enzyme from F. pinicola, which was cultured on softwood mixture as a carb...

This study investigated on enzyme production in the digestive organs of the native termite (Reticulitermes speratus) in Milyang, Korea. Four types of major cellulases [EG (endo-1,4-{\beta}-glucanase), BGL ({\beta}-glucosidase), CBH (cellobiohydrolase) and BXL ({\beta}-1,4-xylosidase)] were present in the digestive organs of the termite. The strong...

A highly efficient β-1,4-glucosidase (BGL) secreting strain, Stereum hirsutum SKU512, was isolated and identified based on morphological features and sequence analysis of internal transcribed spacer rDNA. A BGL containing a carbohydrate moiety was purified to homogeneity from S. hirsutum culture supernatants using only a single chromatography step...

A high β-glucosidase (BGL)-producing strain was isolated and identified as Penicillium pinophilum KMJ601 based on its morphology and internal transcribed spacer rDNA gene sequence. Under the optimal culture conditions, a maximum BGL specific activity of 3.2 U ml−1 (83 U mg-protein−1), one of the highest levels among BGL-producing microorganisms was...

Cellulase from Formiptosis pinicola KMJ812 is an efficient cellulose degradation enzyme complex, especially with a high -glucosidase activity. In this study, the change in enzymatic characteristics by immobilization and the reduction of immobilized enzyme activity by repeated usages were evaluated using cellulases from F. pinicola KMJ812. Among tes...

In the enzymatic hydrolysis of rice straw and wood meals using extra-cellular enzymes from Fomitopsis palustris, key factors which enhanced the sugar conversion yield were investigated in this work, such as enzyme production and enzyme reaction conditions, surfactant effects, and the surface structure of substrates. F. palustris cultured with softw...

The termite is a good model of symbiosis between microbes and hosts and possesses an effective cellulose digestive system. Oxygen-tolerant bacteria, such as Dyella sp., Chryseobacterium sp., and Bacillus sp., were isolated from Reticulitermes speratus gut. Notably, the endo-β-1,4-glucanase (EG) activity of all 16 strains of isolated bacteria was lo...

Reticulitermes speratus is commonly found in Asia, including Korea and Japan. We recently analyzed the 5` region of mitochondrial cytochrome c oxidase subunit I to perform a phylogenetic analysis of R. speratus KMT1, isolated in Seoul, Korea. Our results, using COXI, suggest that the taxonomy of R. speratus should be reconsidered with regard to the...

This study was to investigate the enzymatic hydrolysis of cellulose using the cellulase from whole body of the native termite collected in Milyang-si, Kyungsangnamdo, Korea. In the results, optimal temperature and pH for the enzyme of native termites were 45^{\circ}C and pH 5.5 for both endo-{\beta}-1, 4-glucanase and {\beta}-glucosidase. Enzyme ac...

The high costs for ethanol production with lignocellulosic biomass as a second generation energy materials currently deter commercialization of lignocellulosic biomass, especially wood biomass which is considered as the most recalcitrant material for enzymatic hydrolysis mainly due to the high lignified structure and the nature of the lignin compon...

A beta-1,3-1,4-glucanase from the fungus Laetiporus sulphureus var. miniatus was purified as a single 26 kDa band by ammonium sulfate precipitation, HiTrap Q HP, and UNO Q ion-exchange chromatography, with a specific activity of 29 U/mg. The molecular mass of the native enzyme was 52 kDa as a dimer by gel filtration. beta-1,3-1,4-Glucanase showed o...

A novel endo-beta-1,4-glucanase (EG)-producing strain was isolated and identified as Penicillium pinophilum KMJ601 based on its morphology and internal transcribed spacer (ITS) rDNA gene sequence. When rice straw and corn steep powder were used as carbon and nitrogen sources, respectively, the maximal EG activity of 5.0 U mg protein(-1), one of the...

A recombinant beta-glucosidase from Caldicellulosiruptor saccharolyticus DSM 8903 with a specific activity of 13 U/mg was purified by heat treatment and His-Trap affinity chromatography and identified as a single 54 kDa band on SDS-PAGE. The molecular mass of the native enzyme was 108 kDa as a dimer by gel filtration. beta-Glucosidase showed optimu...

An efficient ß-1,4-glucosidase (BGL) producing strain, Fomitopsis pinicola KMJ812, was isolated and identified based on morphological features and sequence analysis of internal transcribed spacer rDNA. An extracellular BGL was purified to homogeneity by sequential chromatography of F. pinicola culture supernatants on a DEAE-sepharose column, a gel...

Lignocellulosic biomass is the most abundant raw material for bioconversion in many country. However the high costs for pretreatment and enzymatic hydrolysis currently deter commercialization of lignocellulosic biomass, especially wood biomass which is considered as the most recalcitrant material for enzymatic hydrolysis mainly due to the high lign...

An endoglucanase that is able to degrade both crystalline and amorphous cellulose was purified from the culture filtrates of the brown-rot fungus Fomitopsis pinicola grown on cellulose. An apparent molecular weight of the purified enzyme was ∼32 kDa by SDS-PAGE analysis. The enzyme was purified 11-fold with a specific activity of 944 U/mg protein a...

The brown-rot basidiomycete Fomitopsis palustris is known to degrade crystalline cellulose (Avicel) and produce three major cellulases, exoglucanases, endoglucanases, and beta- glucosidases. A gene encoding endoglucanase, designated as cel12, was cloned from total RNA prepared from F. palustris grown at the expense of Avicel. The gene encoding Cel1...

Two endoglucanases with processive cellulase activities, produced from Fomitopsis palustris grown on 2% microcrystalline cellulose (Avicel), were purified to homogeneity by anion-exchange and gel filtration column chromatography systems. SDS-PAGE analysis indicated that the molecular masses of the purified enzymes were 47 kDa and 35 kDa, respective...

In order to investigate the extent of fixation of new water-borne preservatives in blocks of pinus densiflora sapwoods the streaming potential through the column with preservative treated wood was measured at various conditions. The rates of leachability of ingredients in the treated wood were estimated by the measurement of electric conductivity o...