Ye Xia

The Ohio State University | OSU · Department of Plant Pathology

Glycerol-3-phosphate (G3P) is an important metabolite that contributes to the growth and disease-related physiologies of prokaryotes, plants, animals and humans alike. Here we show that G3P serves as the inducer of an important form of broad-spectrum immunity in plants, termed systemic acquired resistance (SAR). SAR is induced upon primary infectio...

Systemic acquired resistance (SAR) is a form of defense that provides resistance against a broad spectrum of pathogens in plants. Previous work indicates a role for plastidial glycerolipid biosynthesis in SAR. Specifically, mutations in FATTY ACID DESATURASE7 (FAD7), which lead to reduced trienoic fatty acid levels and compromised plastidial lipid...

Systemic acquired resistance (SAR), initiated by a plant upon recognition of microbial effectors, involves generation of a mobile signal at the primary infection site, which translocates to and activates defense responses in distal tissues via unknown mechanism(s). We find that an acyl carrier protein, ACP4, is required to perceive the mobile SAR s...

Pseudomonas syringae and Botrytis cinerea cause destructive bacterial speck and grey mold diseases in many plant species, leading to substantial economic losses in agricultural production. Our study discovered that the application of Bacillus proteolyticus strain OSUB18 as a root-drench enhanced the resistance of Arabidopsis plants against P. syrin...

Trichoderma is a genus of wood-decaying fungi generally found in soil (Druzhinina and Kubicek 2005). Trichoderma crassum was confirmed to be a sister species to T. virens according to the molecular sequencing results (Chaverri et al. 2003). A foliar disease with ~70% incidence on Solanum lycopersicum was observed in a greenhouse at The Ohio State U...

Plants evolve a prompt and robust immune system to defend themselves against pathogen infections. Pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) is the first battle layer activated upon the PAMP’s perception, which leads to multiple defense responses. The plasma membrane (PM) H+-ATPases are the primary ion pumps to create and...

Plant fatty acids (FAs) and lipids are essential in storing energy and act as structural components for cell membranes and signaling molecules for plant growth and stress responses. Acyl carrier proteins (ACPs) are small acidic proteins that covalently bind the fatty acyl intermediates during the elongation of FAs. The Arabidopsis thaliana ACP fami...

We report here the genome sequence of Bacillus sp. RRD69, a plant-growth-promoting bacterial endophyte isolated from switchgrass plants grown on a reclaimed coal-mining site in Kentucky. RRD69 is predicted to contain 3,758 protein-coding genes, with a genome size of 3.715 Mbp and a 41.41% GC content. [Formula: see text] Copyright © 2021 The Author(...

The cuticle is the outermost layer of plant aerial tissue that interacts with the environment and protects plants against water loss and various biotic and abiotic stresses. ADP ribosylation factor guanine nucleotide exchange factor proteins (ARF-GEFs) are key components of the vesicle trafficking system. Our study discovers that AtMIN7, an Arabido...

Straw return is an effective strategy to alleviate soil-borne diseases. Though watermelon Fusarium wilt is a severe soil-borne disease, the effect of wheat straw on the disease remains unclear. Thus, we investigated the effects of wheat straw on soil bacterial and fungal communities by adding wheat straw to consecutive watermelon soil in the greenh...

Bacterial spot caused by Xanthomonas spp. is one of the major diseases in tomatoes. X. perforans is the main pathogen of bacterial spot on tomato in Florida. Currently, application of copper fungicides is the primary measure to manage this disease. However, the development of copper resistance in X. perforans and accumulation of copper in the envir...

Sustainability in crop production has emerged as one of the most important concerns of present era's agricultural systems. Plant growth promoting bacteria (PGPB) has been characterized as a set of microorganisms used for enhancing plant growth and a tool for biological control of phytopathogens. However, the inconsistent performance of these bacter...

Bacterial spot of tomato (BST), caused by Xanthomonas perforans, is one of the most serious diseases in fresh market tomato production in Florida, and currently available management measures are largely ineffective or insufficient. Using small molecule compounds has been shown to be promising in management of BST. Carvacrol is a plant-derived small...

The potential of plant growth regulating microorganisms present in the soil can be explored towards the purpose of identifying salt tolerant strategies and crop cultivars. Current study was designed to elucidate the capabilities of salt stress tolerant plant growth promoting rhizobacteria (PGPR) Bacillus siamensis (PM13), Bacillus sp. (PM15) and Ba...

We report here the genome assembly and analysis of Microbacterium strain sp. LKL04, a Gram-positive bacterial endophyte isolated from switchgrass plants ( Panicum virgatum ) grown on a reclaimed coal-mining site. The 2.9-Mbp genome of this bacterium was assembled into a single contig encoding 2,806 protein coding genes.

The potential of plant growth regulating microorganisms present in the soil can be explored towards the purpose of identifying salt tolerant strategies and crop cultivars. Current study was designed to elucidate the capabilities of salt stress tolerant plant growth promoting rhizobacteria (PGPR) Bacillus siamensis (PM13), Bacillus sp. (PM15) and Ba...

SNPs and single base pair (SBP) insertion/deletions (indels) are not only the most abundant genetic markers for genetic mapping and breeding selection, but also always occur in the mutants generated from chemical mutagenesis or CRISPR/Cas9-mediated genome editing. Most of the current SNP and SBP indel genotyping methods are time-consuming and/or re...

We report here the improved draft genome sequence of Bacillus sp. strain YF23, a bacterium originally isolated from switchgrass ( Panicum virgatum ) plants and shown to exhibit plant growth-promoting activity. The genome comprised 5.82 Mbp, containing 5,933 genes, with 193 as RNA genes, and a GC content of 35.10%.

We report here the improved draft genome sequence of Pseudomonas poae strain A2-S9, a bacterium that was originally isolated from switchgrass plants and exhibited the capacity for plant growth promotion. Its genome has a size of 6.68 Mbp and a GC content of 61.3%. The genome encodes 6,022 predicted protein-coding genes.

Wheat powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is considered a major wheat leaf disease in the main wheat producing regions of the world. Although many resistant wheat cultivars to this disease have been developed, little is known about their resistance mechanisms. Pm40 is a broad, effective resistance gene against powdery mi...

Companion cropping with wheat (Triticum aestivum L.) can enhance watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] wilt disease resistance against Fusarium oxysporum f. sp. niveum. However, the mechanism of resistance induction remains unknown. In this study, the effects of microbial community dynamics and the interactions between wheat and w...

As compared to organic farming system, conventional farming system relies on higher inputs of synthetic agrochemicals, which may reduce the abundance, diversity, and beneficial effects of plant endophytic fungal communities. This study compares the diversity and abundance of culturable endophytic fungal communities associated with four plant specie...

In land plants the cuticle is the outermost layer interacting with the environment. This lipophilic layer comprises the polyester cutin embedded in cuticular wax; and it forms a physical barrier to protect plants from desiccation as well as from diverse biotic and abiotic stresses. However, the cuticle is not merely a passive, mechanical shield. Th...

Plants have a diverse endophytic microbiome that is functionally important for their growth, development, and health. In this study, the diversity and specificity of culturable endophytic fungal communities were explored in one of the most important biofuel crops, switchgrass plants (Panicum virgatum L.), which have been cultivated on a reclaimed c...

The growth performance of switchgrass on a coal-mining site. (A) Swtichgrass grown on part1 with better vigorous growth. (B) Swtichgrass grown on part 2 with poor growth performance. (PDF)

ITs sequences and NCBI accession number of the fungal strains listed in S2 Table. (PDF)

Programmed cell death (PCD) plays critical roles in plant immunity but must be regulated to prevent excessive damage. The E3 ubiquitin ligase SPL11 negatively regulates PCD and immunity in plants. We show that SPL11 cell-death suppressor 2 (SDS2), an S-domain receptor-like kinase, positively regulates PCD and immunity in rice by engaging and regula...

Plants have a diverse internal microbial biota that has been shown to have an important influence on a range of plant health attributes. Although these endophytes have been found to be widely occurring, few studies have correlated agricultural production practices with endophyte community structure and function. One agricultural system that focuses...

Plantshaveadiverseinternalmicrobialbiotathathasbeenshowntohaveanimportantinfluenceonarangeofplanthealthattributes.Althoughtheseendophyteshavebeenfoundtobewidelyoccurring,fewstudieshavecorrelatedagriculturalproductionpracticeswithendophytecommunitystructureandfunction.Oneagriculturalsystemthatfocusesonpreservingandenhancingsoilmicrobialabundanceandb...

The plant galactolipids monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) have been linked to the anti-inflammatory and cancer benefits of a green leafy vegetable diet in humans due to their ability to regulate the levels of free radicals like nitric oxide (NO). Here, we show that DGDG contributes to plant NO as well as sali...

Plant cell walls provide physical strength, regulate the passage of bio-molecules, and act as the first barrier of defense against biotic and abiotic stress. In addition to providing structural integrity, plant cell walls serve an important function in connecting cells to their extracellular environment by sensing and transducing signals to activat...

In plants, cellulose biosynthesis is an essential process for anisotropic growth and therefore is an ideal target for inhibition. Based on the documented utility of small-molecule inhibitors to dissect complex cellular processes we identified a cellulose biosynthesis inhibitor (CBI), named acetobixan, by bio-prospecting among compounds secreted by...

Switchgrass (Panicum virgatum L.) is a perennial warm season grass that is native to the plains of North America and is widely grown as a forage, bioenergy or groundcover crop. Despite its importance, a bottleneck in switchgrass production is poor seedling vigor, which as a perennial crop represents an important time for management. Herein, data id...

Fatty acids (FA) and lipids are well known regulators of plant defense. Our previous studies have shown that components of prokaryotic (plastidal) FA biosynthesis pathway regulate various aspects of plant defense. Here, we investigated the defense related roles of the soluble acyl CoA binding proteins (ACBPs), which are thought to facilitate the in...

Glycerol-3-phosphate (G3P), a conserved three-carbon sugar, is an obligatory component of energy-producing reactions including glycolysis and glycerolipid biosynthesis. G3P can be derived via the glycerol kinase-mediated phosphorylation of glycerol or G3P dehydrogenase (G3Pdh)-mediated reduction of dihydroxyacetone phosphate. Previously, we showed...

Author Summary Salicylic acid and enhanced disease susceptibility 1 are important components of resistance gene-mediated defense signaling against diverse pathogens in a variety of plants. Present understanding of plant defense signaling pathways places salicylic acid and enhanced disease susceptibility 1 downstream of resistant protein activation....

Interaction phenotypes of AvrRPT2 or AvrRPS4 expressing P. syringae with eds1 sid2 plants. (A) Photograph showing phenotypes produced upon infiltration of 105 CFU/ml bacteria (AvrRPT2). The leaves were photographed at 3 days post inoculation (dpi). The mock- or pathogen-inoculated EDS1 SID2 F2 plants showed absence of any visible symptoms in respon...

Primer sequences used to amplify various genes. (0.04 MB DOC)

FA composition from leaf tissues of SSI2 (Col-0), eds1, sid2, ssi2, ssi2 eds1, ssi2 sid2, and ssi2 eds1 sid2 plants. All measurements were made on 4-week-old plants grown at 22°C and data are described as mol%±SD calculated for a sample size of six. (0.06 MB DOC)

FA composition from leaf tissues of SSI2 (Col-0), ssi2, ssi2 eds1, fad7, ssi2 fad7, ssi2 eds1 fad7, fad7 fad8, ssi2 fad7 fad8 and ssi2 eds1 fad7 fad8 plants. All measurements were made on plants grown at 22°C and data are described as mol%±SD calculated for a sample size of six. nd, not detected. (0.07 MB DOC)