Yaschar KabiriTechnische Universität München | TUM · Institut für Toxikologie und Umwelthygiene
Yaschar Kabiri
M.Sc. (Toxicology)
About
12
Publications
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Introduction
Additional affiliations
Education
October 2013 - November 2015
February 2008 - February 2013
Publications
Publications (12)
In Wilson disease (WD), functional loss of ATPase copper-transporting β (ATP7B) impairs biliary copper excretion, leading to excessive copper accumulation in the liver and fulminant hepatitis. Current US Food and Drug Administration- and European Medicines Agency-approved pharmacological treatments usually fail to restore copper homeostasis in pati...
In-depth analysis of the mitochondrial proteome can be greatly improved by analyzing isolated mitochondria instead of whole cells. However, isolation of sufficient amounts of mitochondria from cell culture has proven to be notoriously difficult due to small sample size. Thus, we have developed a reproducible, controllable, and highly customizable m...
The increasingly aged human population (mainly in developed countries) represents a significant scientific achievement and privilege associated with medical, social, and economic progress. However, it also poses several challenges to national health and social care systems. The uncoupling of biological evolution with the vast and fast technical pro...
Liver cancers, including hepatocellular carcinoma (HCC), are the second most lethal cancers worldwide and novel therapeutic strategies are still highly needed. Recently, the endolysosomal cation channel TRPML1 has gained focus in cancer research representing an interesting novel target. We utilized the recently developed isoform-selective TRPML1 ac...
Augmenter of liver regeneration (ALR) is a critical multi-isoform protein with its longer isoform, located in the mitochondrial intermembrane space, being part of the mitochondrial disulfide relay system (DRS). Upregulation of ALR was observed in multiple forms of cancer, among them hepatocellular carcinoma (HCC). To shed light into ALR function in...
Isolation of mitochondria is a crucial method for examining molecular details of this organelle’s manifold functions. Historically, mitochondrial isolations required large amounts of sample material which impeded their isolation from cultured cells. We have therefore developed a method allowing for controlled and reproducible isolation of intact an...
Developmental neurotoxicity (DNT) testing performed in rats is resource-intensive (costs, time, animals) and bears the issue of species extrapolation. Thus, reliable alternative human-based approaches are needed for predicting neurodevelopmental toxicity. Human induced pluripotent stem cells (hiPSCs) represent a basis for an alternative method poss...
Questions
Questions (3)
Hey everybody,
I'm currently trying to treat an in vitro culture with varying lead concentrations.
The problem is that I can't think of a way to get meaningful amounts of "biovailable" lead into solution. My cell culture media (regular DMEM) contains phosphate and lead phosphate has a terrible solubility product (in the nM range if I remember correctly?).
So that means that either the entire lead precipitates as insoluble phosphate or I increase the concentration until all phosphate is bound. But that means my cells will die from phosphate starvation.
This problem will occur with all lead salts. So I considered organic lead compounds.
The "classical" tetraethyl lead is basically water insoluble so I guess that one is out. Another one would be lead acetate. Now from what I've read lead acetate does not have the properties of a typical salt, it doesn't have an "ionic" character. Is that true? So would lead acetate not form an insoluble phosphate precipitate?
I really don't want to order yet another lead compound that will sit in our poison cabinet as a CMR substance for many years so any hints would be greatly appreciated.
Thanks in advance,
Yaschar
They offer some assays I find quite interesting, however they are pretty expensive. I've never heard of the company before so I looked around a bit.
On the one hand their manuals are full of typos which is weird for a CA based company... on the other hand there are some papers where their assays were used and these papers look pretty believable.
So has anyone used their assays and can share some thoughts on reliability/usability of these? I'm still a bit sceptical because they seem to offer a ridiculous amount of products, yet they are a rather small company.
Thanks and best regards,
Yaschar
Hi everyone,
I'm currently rewriting an old protocol I once did. The procedure is the following:
- Dissolve decylubiquinone in acidic EtOH
- Add a tiny amount of NaBH4
- Vortex until solution becomes colorless
- Add cyclohexane, vortex
- Add a little H2O, vortex, allow for phases to separate
- Remove organic phase, add more cyclohexane, vortex
- Allow to settle, remove organic phase and pool both organic phases
- carefully dry under N2, resolve in acidic EtOH, analyze and quantify
Now the thing is we're moving to a new lab and we've got an ample supply of n-hexane. I don't want to order a bottle of cyclohexane because there's no other use for it outside of this reaction which is very rarely done.
I see no problem in substituting the cyclohexane with n-hexane here, or am I getting something wrong?
Cheers and thank you
Yaschar