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Yaroslav Ivanovych

Yaroslav Ivanovych
Yuriy Fedkovych Chernivtsi National University · Yuriy Fedkovych Chernivtsi National University

PhD

About

21
Publications
3,898
Reads
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27
Citations
Additional affiliations
January 2017 - present
Industry / medical labs & clinics
Position
  • Molecular Biologist, Senior Geneticist, Senior Molecular Biologist
Description
  • • Implementation and conducting routine NGS with Ion AmpliSeq™ CarrierSeq™ expanded carrier screening Panel with subsequent software data analisys and reporting (NGS: ThermoFisher Ion Torrent S5); • Routine implementation of new diagnostics kits for hereditary diseases, some infectious pathogens and preparing new templates for the results reporting; • Providing technical support, continuous operation of equipment and deal with reclamation; • Management the Molecular Genetics Lab. Sector team.
April 2016 - February 2017
Institute of Horticulture NAAS, Kyiv, Ukraine
Position
  • Junior Research Fellow
Description
  • • Research in the field of plant genetics: genetic diversity, genetic structure, phylogenetic relationship and population genetics of Ukrainian cultivars with molecular markers (SSR, PCR-RFLP, ISSR, IRAP, REMAP). Analysis of literary data, writing of scientific articles, reports and preparation of presentations; • Working on PhD-thesis in the field of molecular genetics "Genetic fingerprinting for the marker-assisted selection of sweet cherry (Prunus avium L.) cultivars of Ukrainian breeding";
January 2013 - February 2017
Ukrainian Academy of Agrarian Sciences
Position
  • Researcher
Education
January 2013 - December 2015
Institute of Horticulture of the NAAS of Ukraine / Institute of Food Biotechnology and Genomics NAS of Ukraine
Field of study
  • Molecular Genetics
September 2007 - May 2012
Chernivtsi National University
Field of study
  • Biology / Genetics

Publications

Publications (21)
Article
Full-text available
Microsatellite (SSR) markers were used to characterise 23 sweet cherry cultivars of Ukrainian, and four cultivars of non-Ukrainian, origin. To assess their genetic diversity and relatedness, 11 pairs of primers were applied to microsatellite loci, resulting in amplification of 66 SSR alleles. The mean value of the number of different alleles, and t...
Article
Full-text available
ISSR-PCR markers were used to assess genetic diversity and to elucidate relatedness among 21 Ukrainian and three West-European sweet cherry cultivars that are widely cultivated in Ukraine. The discriminatory potential was tested for 11 ISSR-PCR primers, which produced 193 amplicons. UBC 835, 836, 841, and 881 were identified as the best primers sui...
Article
Full-text available
Aim. Ukrainian breeders have created a large number of sweet cherry cultivars, which still remain almost unexplored at the molecular level. The aim of our study was to identify the self-incompatibility alleles (S-alleles) in Ukrainian sweet cherry cultivars and landraces, and to elucidate, to which cross-incompatibility group the cultivars belong....
Article
Full-text available
Aim. In recent decades, Ukrainian breeders have created a large number of sweet cherry cultivars. Further progress in the breeding of sweet cherry requires a broad involvement of molecular methods. Especially important is the development of methods for the identification of genes / alleles that control economically valuable traits. The goal of the...
Thesis
Full-text available
The study of the genetic diversity of Ukrainian sweet cherry cultivars using DNA markers is important for targeted breeding research, control of cultivar identity and genetic stability of clones, management of collections of genetic resources and protection of breeders' rights. The genetic fingerprinting of Ukrainian sweet cherry cultivars and acce...
Conference Paper
Full-text available
Aim. In flowering plants, the size and weight of fruits are mainly under the control of CSR (cell size regulator) and CNR (cell number regulator) genes. Among them, the CNR12 gene is the best characterized. In sweet cherries, three allelic variants of this gene were described: PavCNR12-1,-2, and-3, which differ in fourteen SNPs in noncoding regions...
Presentation
Full-text available
Thesis defence presentation "Genetic fingerprinting for the marker-assisted selection of Ukrainian sweet cherry (Prunus avium L.) cultivars"
Conference Paper
Full-text available
Sweet cherry (Prunus avium L.) is one of the most important industrial fruit crops in Ukraine. Over the past decades, Ukrainian breeders have created a large number of sweet cherry cultivars, which are promising for industrial cultivation and competitive on the global market. Discovery and characterization of genes related to economically valuable...
Article
Full-text available
Aim. To estimate the possibilities of using the promising apple cultivars, created by the Institute of Horticulture (IH) NAAS, in the breeding programs according to the availability of valuable allele variants of gene Rf, related to red color of apple fruit. Methods. Polymerase chain reaction, electrophoresis in agarose gel, the evaluation of pheno...
Technical Report
Full-text available
The main focus of the STSM was the study of the genetic diversity of Ukrainian sweet cherry varieties and landraces using SSR markers. The set of markers includes some which are linked with economically important traits (e.g.: fruit weight) and applicable for marker-assisted selection (MAS) strategies. The second important goal was to make molecula...
Chapter
Full-text available
3. Біотехнології в садівництві 3.1. Біотехнологічні методи діагностики вірусних, бактеріальних та грибних патогенів плодових, ягідних та декоративних культур в системі виробництва здорового садивного матеріалу 3.2. Біотехнологічні методи прискореного розмноження та оздоровлення плодових, ягідних та горіхоплідних культур 3.3. Генетична паспортизація...
Conference Paper
Full-text available
Sweet cherry is one of the important industrial fruit crop in Ukraine. Over the past decades, Ukrainian breeders have created a large number of sweet, sour and duke cherries’ varieties, which are promising for industrial cultivation and competitive on the global market. To date, the majority of the sweet cherry varieties of Ukrainian origin remain...
Conference Paper
Full-text available
Fruit diameter is an important factor in consumer preference and large determinant in the price formation. It was devised an alternative method of PavCNR12 alleles identification with using method Cleaved Amplified Polymorphic Sequences (CAPS). A method involves amplification of the target area using primers CNR12-C2-F and CNR12-C2-R with subsequen...
Article
Full-text available
Qualitative improvement and a significant acceleration of breeding sweet cherry possible under using marker assisted selection. The genetics research of agronomically-important traits in sweet cherry was actively continues in the last decade. Many molecular studies are still ongoing, but already known genes that determine self-comtability, self-inc...
Conference Paper
Full-text available
In this work the genome fingerprinting of Ukrainian sweet cherry cultivars was conducted for their discrimination, clarifying the relationship between varieties, germplasm systematization and protection of breeder’s rights. For these purpose three UBC primers (ISSR-PCR) was tested. It was assessed discriminative characteristics of UBC-markers using...
Article
Full-text available
The using of uniform plant material with confirmed cultivar identity is the most critical factor for creating gardens of intensive type. The efficiency of identification method and analysis of genetic diversity between different fruit cultivars with traditional phenotypical traits are insufficient taking into account its high dependence from enviro...
Conference Paper
Full-text available
Profiling genome of sweet cherry is the basis for determination of cultivars, hybrids and their relationships, systematization of germplasm and protection of breeder’s rights. In investigation primer to TRIM retrotranposons was tested for fingerprinting Ukrainian sweet cherry cultivars. Despite this demonstrated a low level of intraspecific polymor...
Article
Full-text available
The 5S ribosomal DNA polymorphism of Polyommatus icarus (Insecta, Lepidoptera). The5S rDNA of Polyommatus icarus was cloned and sequenced in order to evaluate the potential of this locus as a molecular marker used in the taxonomy of butterflies. The novel data were compared with the 5S rDNA sequences obtained earlier for other species and other pop...

Questions

Question (1)
Question
I was used AnalyticJena qTOWER 2.2 and supplied soft.
We are using one of commercial kits for Gilbert disease diagnostics, SNP detection. The kit is not validated on our instrument. In the beginning of plots are strange decreasing signal, but less in positive control, PC (commercially provided). In general data visualization looks poor. For comparison I have add screens of "correct" run and current one "incorrect". I suppose some inhibition of reaction in analyzed samples. Amount of added DNA was 125-180 ng per reaction (final vol 25 ul).
Thanks in advance!

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Projects

Project (1)
Project
Studying the sweet cherry cultivars of Ukrainian breed