Yanyan Wang

Yanyan Wang
Tianjin University | tju · Department of Precision Instrument Engineering

PhD

About

75
Publications
10,248
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865
Citations
Introduction
Yanyan Wang currently works at the Department of Precision Instrument Engineering, Tianjin University. Yanyan does research in biosensing, Electrochemistry and Nanotechnology. Their current project is micro/nano biosensors

Publications

Publications (75)
Article
The carbohydrate-derived lipoic acid derivatives were studied as protein and cell resistant biomaterials. Six types of carbohydrates were examined for their abilities to reduce nonspecific adsorption of human serum and Hela cell using quartz crystal microbalance. Our data suggested that the structures of carbohydrates play an important role in resi...
Article
Full-text available
At the single-cell level, cellular parameters, gene expression and cellular function are assayed on an individual but not population-average basis. Essential to observing and analyzing the heterogeneity and behavior of these cells/clusters is the ability to prepare and manipulate individuals. Here, we demonstrate a versatile microsystem, a stereo a...
Article
The hydrodynamic method mimics the in vivo environment of the mechanical effect on cell stimulation, which not only modulates cell physiology but also shows excellent intracellular delivery ability. Herein, a hydrodynamic intracellular delivery system based on the gigahertz acoustic streaming (AS) effect is proposed, which presents powerful targete...
Article
The transport, enrichment, and purification of nanoparticles are fundamental activities in the fields of biology, chemistry, material science, and medicine. Here, we demonstrate an approach for manipulating nanospecimens in which a virtual channel with a diameter that can be spontaneously self-adjusted from dozens to a few micrometers based on the...
Article
Cell mechanical motion is a key physiological process that relies on the dynamics of actin filaments. Herein, a localized shear-force system based on gigahertz acoustic streaming (AS) is proposed, which can simultaneously realize intracellular delivery and cellular mechanical regulation. The results demonstrate that gold nanorods (AuNRs) can be del...
Article
Currently, researches on nanomaterials have been restricted by slow and multistep synthesis procedures. Herein, we demonstrate an ultrafast, one step method of purification and delivery of quantum dots into living cells, actuated by the acoustic streaming (AS) produced through a gigahertz resonator. Results demonstrate that the impurities in the ca...
Preprint
At the single-cell level, cellular parameters, gene expression and function are assayed on an individual but not population-average basis. Essential to observing and analyzing the heterogeneity and behavior of these cells/clusters is the ability to prepare and manipulate individual. Here, we demonstrate a versatile microsystem, a stereo acoustic st...
Article
The development of rapid and efficient tools to modulate neurons is vital for the treatment of nervous system diseases. Here, a novel non-invasive neurite outgrowth modulation method based on a controllable acoustic streaming effect induced by an electromechanical gigahertz resonator microchip is reported. The results demonstrate that the gigahertz...
Article
The important role of BV in clinical diagnostics of liver-related diseases has been established in veterinary medicine. However, the sensitivity and selectivity of the current BV assays remain relatively low compromising its wider application in clinical diagnosis. Herein, we developed a rapid and sensitive BV-detecting biosensor based on a novel f...
Article
Full-text available
Hydrodynamic force loading platforms for controllable cell mechanical deformation play an essential role in modern cell technologies. Current systems require assistance from specific microstructures thus limiting the controllability and flexibility in cell shape modulation, and studies on real‐time 3D cell morphology analysis are still absent. This...
Article
Here, we present a high performance uncooled near-infrared (NIR) detector comprising of a giga hertz (GHz) solidly mounted resonator (SMR) and gold nanorods (GNRs) arrays. By coupling the localized surface plasmon resonances of GNRs, the resonator system exhibits optimized optical response to vis-NIR region. Both simulation and experiments demonstr...
Conference Paper
This work reports a novel acoustic resonator system integrated dual functions of biological samples capture and amount monitoring on a single chip. The system could capture samples from nano-sized proteins to micro-sized cells on micro-sized chip precisely with controllable concentration, meanwhile the high sensitivity mass sensing was achieved dur...
Article
In article number 2000364 by Yanyan Wang, Zefang Wang, and co‐workers, an ultrasensitive and rapid thrombin biosensor is developed based on rationally designed trifunctional protein HTs, comprising three functional units, including a far‐red fluorescent protein smURFP, hydrophobin HGFI, and a thrombin cleavage site (TCS). smURFP is used as a detect...
Article
Rapid and sensitive detection of thrombin is imperative for the early diagnosis, prevention, and treatment of thrombin‐related diseases. Here, an ultrasensitive and rapid thrombin biosensor is developed based on rationally designed trifunctional protein HTs, comprising three functional units, including a far‐red fluorescent protein smURFP, hydropho...
Article
Hydrophobins are small, secreted amphiphilic proteins produced by filamentous fungi. Due to their charming ability to self-assemble at different interfaces, several efforts have been made in recent years to produce hydrophobins at a large scale for industrial applications. However, producing soluble and functional hydrophobins in bacterial expressi...
Article
Gravimetric resonators based on Micro/Nanoelectromechanical System (M/NEMS) are potential candidates in developing smaller, less expensive, and higher performance gas sensors. Metal organic frameworks (MOFs), with high surface areas, recently come into focus as advanced nano-porous sensitive materials in micro gravimetric gas sensors. The surface o...
Article
Polyethylene terephthalate (PET) is one of the most widely used plastics in the world. Accumulation of the discarded PET in the environment is creating a global environmental problem. Recently, a bacterial enzyme named PETase was found to have the novel ability to degrade the highly crystallized PET. However, the enzymatic activity of native PETase...
Article
Full-text available
Film bulk acoustic wave resonators have demonstrated great potential in the detection of volatile organic compounds owing to their high sensitivity, miniature size, low power consumption, capacity for integration, and other beneficial characteristics. However, it is necessary to functionalize the surfaces of these resonators to enhance the adsorpti...
Article
In article 1900671, Qiannan Xue, Xuexin Duan, and co‐workers demonstrate a nanoscale impedimetric immunosensor based on biotin doped conductive polymer nanowire arrays which are fabricated through simple solution printing approach. This sensor can be a general platform for various bio‐analytical applications with ultrahigh sensitivity.
Article
Full-text available
Background Controllable and multiple DNA release is critical in modern gene-based therapies. Current approaches require complex assistant molecules for combined release. To overcome the restrictions on the materials and environment, a novel and versatile DNA release method using a nano-electromechanical (NEMS) hypersonic resonator of gigahertz (GHz...
Article
The demand for fast and ultratrace biomarkers detection is increasing in bioanalytical chemistry. In this work, highly ordered nanowires array and sensor integration are achieved with nanoscale printing approach. Negatively charged poly(3,4‐ethylenedioxythiophene)–poly(styrenesulfonate) doped with positively charged PEGylated biotin‐derivatized pol...
Article
The intracellular delivery efficiency of drug-loaded nanocarriers is often limited by biological barriers arising from the plasma membrane and the cell interior. In this work, the entering of doxorubicin (Dox)-loaded mesoporous silica nanoparticles (MSNs) into cytoplasm was acoustically enhanced through direct penetration with the assistance of hyp...
Article
Full-text available
A highly sensitive electrochemical biosensor with a signal amplification platform of electrodeposited gold nanoparticle (AuNP) has been developed and characterized. The sizes of the synthesized AuNP were found to be critical for the performance of biosensor in which the sizes were dependent on HAuCl4 and acid concentrations; as well as on scan cycl...
Article
Hierarchical assemblies of nanomaterial superstructures with controlled orientation affords a multitude of novel properties of plasmonics and broad applications. Yet constructing multi-functional superstructures with nanoparticles positioned in desired locations remains challenging. Herein, gold nanorods (GNRs) assembled in stripe patterns with con...
Article
Full-text available
Rapid and reliable micromixing requires continuous improvement to renovate more powerful microfluidics chip for chemosynthesis, biological assay, and drug purification. In this work, we realized rapid in-situ mixing in droplets on a closed electro-microfluidic chip. Electrowetting and 2.5 GHz acoustic wave streaming were coupled into a monolithic c...
Article
Full-text available
In designing bioassay systems for low-abundance biomolecule detection, most research focuses on improving transduction mechanisms while ignoring the intrinsically fundamental limitations in solution: mass transfer and binding affinity. We demonstrate enhanced biomolecular surface binding using an acoustic nano-electromechanical system (NEMS) resona...
Data
Supplementary figures and tables.
Article
Full-text available
Low water solubility and poor selectivity are two fundamental limitations that compromise applications of near-infrared (NIR) fluorescent probes. Methods: Here, a simple strategy that can resolve these problems simultaneously was developed by using a novel hybrid protein named RGD-HFBI that is produced by fusion of hydrophobin HFBI and arginine-gly...
Article
Full-text available
Quantitative biomarker detection methods featured with rapidity, high accuracy, and label-free are demonstrated for the development of point-of-care (POC) technologies or "beside" diagnostics. Microbead aggregation via protein-specific linkage provides an effective approach for selective capture of biomarkers from the samples, and can directly read...
Article
On-chip integrating several functional components for developing integrated lab-on-a-chip microsystem remains as a challenge. In this work, by employing multiple microelectromechanical resonators both as actuators and sensors, on-chip heating, mixing and chemical reaction monitoring are successfully demonstrated. Mechanism studies using COMSOL simu...
Article
Nonspecific binding (NSB) is a general issue for surface based biosensors. Various approaches have been developed to prevent or remove the NSBs. However, these approaches either increased the background signals of the sensors or limited to specific transducers interface. In this work, we developed a hydrodynamic approach to selectively remove the N...
Article
In article number 1602962, Xuexin Duan and co-workers, explore a novel chemical-free cell poration method – “hypersonic poration”, which is developed to improve the cellular uptake, especially nucleus uptake, using a designed piezoelectric nanoelectromechanical resonator. The hypersound has a typical frequency larger than GHz, which can directly in...
Article
In this work, a smartphone-enabled platform for easy and portably colorimetric analysis of 2,4,6-trinitrotoluene (TNT) using amine-trapped PDMS is designed and implemented. The amine-trapped PDMS is simply prepared by immersing the cured PDMS in aminosilane solutions forming an amine-containing polymer. After contacting with TNT-containing solution...
Chapter
Gold nanorod (GNR) based label-free sensing has been attractive due to its unique property of localized surface plasmon resonance (LSPR). Compared to bulk gold, the SPR of GNRs is more sensitive to the refractive index change caused by biological binding in the close proximity. Numerous studies have reported biological detection in solution based G...
Article
Full-text available
Introducing sulfhydryl groups to biomolecules to functionalize gold nanorods (GNRs) is an attractive method that involves the creation of a strong Au-S bond. Previously, we developed a facile method to functionalize GNR surfaces by thiolating antibodies using Traut's reagent. In the current study, we evaluated several methods for the introduction o...
Data
Additional experimental data.
Article
Efficient delivery of genes and therapeutic agents to the interior of the cell is critical for modern biotechnology. Herein, a new type of chemical-free cell poration method— hypersonic poration—is developed to improve the cellular uptake, especially the nucleus uptake. The hypersound (≈GHz) is generated by a designed piezoelectric nano-electromech...
Article
A novel one-step strategy to exfoliate and functionalize two dimensional layered materials (2DLM) is described to promote the production and performance of the 2DLM for bio-related and molecule adsorption based practical applications. This method applies sonication to the 2DLM dissolved in the aqueous solution of hydrophobin protein, during which p...
Article
This paper reports a surface functionalization strategy for protein detections based on biotin-derivatized poly(L-lysine)-grafted oligo-ethylene glycol (PLL-g-OEGx-Biotin) copolymers. Such strategy can be used to attach the biomolecule receptors in a reproducible way simply by incubation of the transducer element in a solution containing such copol...
Article
The advancement in micro/nanotechnologies has been continuously providing possibilities for inventing novel biochemical sensors. However, variations in the transducer type can cause different sensing results due to the differences in their mechanisms of analyzing biomolecular interactions. In this work, we focused on the comparative analysis of sta...
Article
Full-text available
Controlled drug release has a high priority for the development of modern medicine and biochemistry. To develop a versatile method for controlled release, a miniaturized acoustic gigahertz (GHz) resonator is designed and fabricated which can transfer electric supply to mechanical vibrations. By contacting with liquid, the GHz resonator directly exc...
Article
With the development of microfabrication, enjoying the advantages of high accuracy, small size and biological compatibility, micro electro mechanical system (MEMS)devices have been widely applied in biological and medical treatment. This paper reviews the application development of MEMS devices in drug delivery technology. The fabrication and appli...
Article
Full-text available
Low water solubility and poor membrane permeability are major disadvantages that compromise applications of most fluorescent dyes. To resolve these problems, herein, using Boron-dipyrromethene (BODIPY) as a model fluorescent dye, for the first time, we provide a new strategy for the rapid and efficient production of a water-soluble and membrane-per...
Chapter
The cells are comfortable and functional in microenvironment (in the near)— because extracellular matrix (ECM) not only provides structural support but also promotes physical and chemical cues for them to behave well in the complex environment. This chapter aims to relate the adsorption behavior by electrochemical monitoring of toxin, curvature, an...
Article
A new surface functionalization scheme for nano-Bio field effect transistors (FETs) using biocompatible polyelectrolyte thin films (PET) is developed. PET assemblies on Si nanowires (Si-NWs) are driven by electrostatic interactions between the positively charged polymer backbone and negatively charged Si/SiO2 surface. Such assemblies can be directl...
Article
In this work, a new method to tune the resonant frequency of micro-fabricated resonator using molecular layer-by-layer (LbL) self-assembly approach is demonstrated. By simply controlling the polymer concentration and the number of layers deposited, precisely tuning the frequency of micro-fabricated resonators is realized. Due to its selective depos...
Conference Paper
Gold nanoparticles have shown great potential applications in biomedical diagnosis and therapeutic treatments due to their shape and size-dependent optical properties. However, densely packed CTAB double layer (surfactant used to prevent nanoparticle aggregation) on the nanorod surfaces present challenges for effective biofunctionalization to setup...
Article
Polyaniline (PANI) films were polymerized on glass carbon (GC) electrodes with small molecular dopantshydrochloric, perchloric, sulfuric, methanesulfonic, benzenesulfonic, ρ- toluenesulfonic and large macromolecular size poly(4-styrenesulfonic (PSS)), poly(vinylsulfonic), poly(acrylic), and poly(anilinesulfonic) acids.Theredox electroactiveswere st...
Article
A novel noncovalent approach was developed for the functionalization of multi-wall carbon nanotubes (MWNTs) using the hydrophobin, HFBI. Owing to the amphipathic nature, HFBI can be adopted onto the surface of MWNTs to form HFBI-MWNTs nanocomposite with good dispersion in water. The HFBI-MWNTs nanocomposite was characterized by scanning electron mi...
Article
Multivalent interaction between boronic acids immobilized on quartz crystal microbalance (QCM) sensor surface and the carbohydrates modified Au-nanoparticle (AuNP) has been demonstrated for the development of a sensitive carbohydrate biosensor. Briefly, a boronic acid-containing polymer (boropolymer) as multivalent carbohydrate receptor was oriente...
Article
An oriented glyco-capturing macroligand was synthesized by site-specific immobilization of an O-cyanate chain-end-functionalized boronic acid containing polymer (boropolymer) onto an amine surface. The O-cyanate chain-end-functionalized boropolymer was synthesized by arylamine-initiated cyanoxyl-mediated free-radical polymerization in a one-pot fas...
Article
Full-text available
Hydrophobins are amphiphilic proteins with high surface activity, which can readily adsorb on interfacial surfaces, especially on hydrophobic surfaces. Based on their properties, we used the class I hydrophobin isolated from Grifola frondosa (HGFI) to disperse multi-walled carbon nanotubes (MWCNTs) in water. MWCNTs could be effectively dispersed by...
Article
Many processes in living cells have electrochemical characteristics that are suitable for measurement by potentiometric biosensors. Potentiometric biosensors allow non-invasive, real time monitoring of the extracellular environment changes by measuring the potential at cell/sensor interface. This can be used as an indicator for overall cell cytotox...
Article
Full-text available
A novel amperometric choline biosensor was constructed by alternately assembling poly-( diallyldimethylammoium chloride) (PDDA) and choline oxidase on a platinum electrode modified by multi-walled carbon nanotubes ( MWCNTs ) and three layers of poly-( allylamine) (PAA)/poly-(vinyl sulfate, potassium salt) (PVS). The results showed that MWCNTs could...
Article
A novel amperometric ethanol biosensor was developed based on layer-by-layer (LbL) technique to immobilize alcohol oxidase (AOx) on the Platinum (Pt) electrode surface. First, Pt, glassy carbon (GC), and gold (Au) electrodes were used as base electrodes for constructing ethanol biosensors, and the responses of these biosensors were compared. Second...
Article
Poly(diallyldimethylammonium chloride) (PDDA) was chosen to disperse single-walled carbon nanotubes (SWCNTs). The optimal conditions to prepare stable PDDA–SWCNTs aqueous dispersions were presented. Then, the positively charged PDDA–SWCNTs composite and negatively charged glucose oxidase (GOD) were employed to fabricate multilayer films on platinum...
Article
A novel amperometric ethanol biosensor with high sensibility and good anti-interference property has been developed. In this work, the gelatin embedding