Yannis Kotzamanis

• Ph.D
• Research Director at Hellenic Centre for Marine Research

The valorization of aquaculture/fishery processing by-products, as well as unavoidable/unwanted catches and discards in Greece, is currently an underutilized activity despite the fact that there are several best practices in Northern Europe and overseas. One of the main challenges is to determine whether the available quantities for processing are...

The valorization of aquaculture/fisheries processing by-products, as well as unavoidable/unwanted catches and discards in Greece is currently an underutilized activity despite the fact that there are several best practices in Northern Europe and overseas. One of the main challenges is to determine whether the available quantities for processing are...

Simple Summary Aquaculture is predicted to play a crucial role in providing nutritional and healthy food for people worldwide. Under the Blue Transformation concept, aquaculture should be sustainable and resilient, minimize environmental impacts, and improve biosecurity and disease control. The use of essential oils and extracts of various species...

Marine (blue) biotechnology is an emerging field enabling the valorization of new products and processes with massive potential for innovation and economic growth. In the Mediterranean region, this innovation potential is not exploited as well as in other European regions due to a lack of a clear identification of the different value chains and the...

The marine microalgae Tetraselmis striata was cultivated in drilling waters with different salinities. Growth substrate optimization was performed while the effects of different pH, temperature, photoperiod and CO2 flow rate on biomass productivity and its composition were studied. Results showed that the strain grew better in 2.8% drilling waters...

In the recent years, insect meals have been studied as alternative feed ingredients for aquafeeds, due to their adequate nutrient composition and low ecological footprint. These studies involve nutrient digestibility measurements, as they provide valuable information on the ability of fish to utilize insect meal efficiently. In this context, the cu...

Alternative and sustainable fish diets are required by modern aquaculture. We investigated the possibility of using insect (Tenebrio molitor TM, Hermetia illucens HI or Musca domestica MD) larvae meals (as 19.5% of the feed formulation) to replace 30% of the in the fish meal (FM) in a gilthead seabream (Sparus aurata) feed formulated to contain 65%...

As crude glycerol comprises a potential substrate for microalga fermentation and value added products’ biosynthesis, Auxenochlorella protothecoides was grown on it under heterotrophic and mixotrophic conditions and its growth kinetics were evaluated in a continuous system under steady state conditions. Increasing initial glycerol concentration (fro...

This study examined the modulatory effect of two commercial feed additives, Lumance® (0.2% and 0.5%) and Novigest® (0.4%), on the growth and microscopic structure of the intestine and liver of juvenile gilthead sea bream (Sparus aurata), when added to high (HFM-0) and low fish meal (LFM-0) diets. Lumance® was added only in the HFM-0 diet (HFM-0.2 a...

An 8-week feeding trial was conducted to determine the dietary lysine requirement of greater amberjack, Seriola dumerili. Six experimental diets resulting from a practical basal formulation were produced to containing mainly plant ingredients (25% fish meal) were supplied with graded levels of crystalline L-lysine-HCl and the analysed lysine concen...

Catfish (Siluriformes) are important species for aquaculture worldwide, with an annual production in 2018 of ca. 6 million t. This review focuses on reassessing larval development, first feeding, and early rearing practices of the most important farmed catfish species, along with some candidate species for aquaculture diversification: Pangasianodon...

The aim of this study was to determine the proximate composition and amino acid profile of the edible muscle of European crayfish Astacus astacus L. Animals were collected from Orchomenos region in Central Greece and muscle tissue samples were collected for chemical analysis. Total crude protein and crude lipid content of muscle tissues were determ...

The aim of this study was to determine the proximate composition and amino acid profile of the edible muscle of European crayfish Astacus astacus L. Animals were collected from Orchomenos region in Central Greece and muscle tissue samples were collected for chemical analysis. Total crude protein and crude lipid content of muscle tissues were determ...

Soy protein concentrate (SPC) rarely replaces fishmeal (FM) completely in marine finfish diets without eliciting adverse effects on fish performances. The inclusion of brown seaweed (Sargassum fulvellum) could alleviate the negative effects linked to high SPC dietary inclusion. In the current study, the dietary effects of S. fulvellum inclusion in...

This study was conducted to elucidate the interaction effects of temperature and dietary lipid levels (2 × 2 factorial experiment) on the growth performance, muscle, and liver composition in adult farmed European sea bass (Dicentrarchus labrax). Two groups of fish (190 g; 60 fish per group) were distributed in 12 tanks in triplicates and kept at tw...

Plant-based proteins are increasingly being used in aquafeeds, but one of the limitations is taurine deficiency that can be especially detrimental for carnivorous fish. In this study, taurine supplementation in high plant protein diets (low fish meal, 15%) was investigated on the growth performance and fillet organoleptic characteristics of Europea...

Insects are considered a sustainable alternative protein source in aquaculture diets. So far, most studies regarding the inclusion of inset meals in the diets of farmed fish focus on one insect species individually and they are examining the effect of different insect meal inclusion levels. This is a comparative study of the use of three different...

This study evaluated the effects of taurine supplementation to diets containing a high dietary inclusion of soybean meal and soy protein concentrate on growth performance and fillet quality of juvenile European sea bass (Dicentrarchus labrax). A control diet (C+) was produced containing high levels of fishmeal (30% FM) and soybean meal (20% SBM). T...

White sea bream (Diplodus sargus) and brown meagre (Sciaena umbra) were caught from the wild in Aegean Sea (Greece), and studied for their proximate, fatty acid and amino acid composition to evaluate their nutritional value for human consumption and their potential as candidate fish species for Mediterranean aquaculture diversification. Both specie...

A UPLC-QTOF-MS method for the simultaneous determination of 20 veterinary drug residues and metabolites (tetracyclines, quinolones, sulfonamides and diaminopyrimidines) in edible muscle plus skin tissue of European sea bass (Dicentrarchus Labrax) was developed. For the identification of analytes a positive electrospray ionization quadropole time-of...

The potential use of plant protein (PP) blends (soybean, wheat, rapeseed, corn gluten and wheat gluten) in the diet of juvenile meagre (Argyrosomus regius) was investigated at increasing levels to replace fishmeal (FM) (33% and 56%) in six isonitrogenous (480 g/kg) and isoenergetic (22 MJ/kg) diets, which were supplemented with crystalline lysine....

Background: Danofloxacin is a synthetic antibacterial agent with broad spectrum antibacterial and antimycoplasmal activity which is widely used in aquaculture. Methods: A UPLC (Ultra Performance Liquid Chromatography) method was developed and validated for the determination of danofloxacin (Dano) and its major metabolite N-desmethyl danofloxacin (N...

Residue depletion of danofloxacin was investigated in European sea bass after a multiple (5 days) in-feed administration of danofloxacin mesylate (10 mg/kg bw per day) under experimental field conditions at water temperature of 16 °C and 27 °C. Ten fish per sampling point were examined during and after treatment. Samples of muscle plus skin in natu...

Wine grape pomace is an abundant by-product of the wine-industry in Greece. It contains resveratrol (3,5,4'-trihydroxy-trans-stilbene), which is found in the skin of red grapes, a type of natural phenol which has been suggested to have many benefits in human health (Athar et al. 2007). Resveratrol in common with other polyphenols, was found to be a...

This study aimed to identify and characterize the cellular and metabolic response in vital organs of Dicentrarchus labrax, after feeding on soy-based diets enriched with different taurine concentrations (T0.2, T0.5, T1.0 w/w). Molecular responses (expression of Hsp70, Hsp90, phosphorylation of p38 mitogen-activated protein kinase-p38MAPK and extrac...

Dicentrarchus labrax were fed from 10 to 40 days posthatching with six microdiets differing in the inclusion level (60 g kg-1 and 120 g kg-1) and type of protein hydrolysate (PH; yeast, YPH; pig blood, PBPH; pig red blood cells, PRBCPH). A microdiet containing 120 g kg-1 fish PH (FPH) was used as a control. PH differed in their amino acid (AA) prof...

The potential of rapeseed protein concentrate as fish meal alternative in diets for rainbow trout (initial average weight 37.8 +/- A 1.4 g) was evaluated. Nine experimental tanks of a freshwater flow-through system were stocked with 12 fish each. Triplicate groups of fish received isonitrogenous (47.9 +/- A 0.5% CP) and isoenergetic (22.4 +/- A 0.2...

In this study, we have evaluated the incorporation of two types of protein hydrolysates at 9 and 12% levels of inclusion, one from yeast (Saccharomyces cerevisiae, YPH) and another one from pig blood (PBPH), in microdiets for gilthead sea bream (Sparus aurata) larvae, and compared these results to a microdiet containing fish protein hydrolysate and...

Abstract Four experimental diets with different inclusion levels of plant proteins and fish protein hydrolysates were compared with a commercial diet for meagre (Argyrosomus regius) ongrowing at optimal and suboptimal water temperature. Results in terms of growth in length and weight, conversion efficiency, dietary feed intake and utilization, body...

Several authors have previously demonstrated that the number of the skin mucous cells of fish is affected by many stressors. In the present study, two experiments were conducted in order to examine the effects of two common environmental conditions on the morphology of skin of sea bass and particularly on the number and diameter of skin mucous cell...

Three legumes [field peas (P), chickpeas (CP) and faba beans (B)] were evaluated at two inclusion levels; 170 (L) and 350 g kg− 1 (H) in a 14-week experiment with triplicate groups of 97.9 ± 6.1 g European seabass. A control diet included wheat meal, fish meal (FM) and a mixture of plant ingredients as protein sources. Diets, processed in a twin-sc...

The effects of long term feeding (6 months) of commercial diets with low fish meal content and high levels of vegetable oils (69% fish oil substitution level) were determined in gilthead sea bream (110 g). A control diet containing South American fish oil (FO) was evaluated against feeds with either soybean oil (SO), palm oil (PO) or rapeseed oil (...

The digestive physiology of common dentex was studied by assessing the activity of different pancreatic (trypsin, chymotrypsin, amylase and lipase), intestinal (alkaline phosphatase, aminopeptidase N, maltase and leuncine–alanine peptidase) and gastric (pepsin) enzymes from hatching until the juvenile stage (50 dph at 19 °C). Enzymes involved in th...

Several nutritional studies have found a direct effect of several vitamins in chondrogenic and osteogenic development during early life stages of marine fish species. In the present study, the effect of vitamin A (VA) in gilthead sea bream skeletogenesis was evaluated by means of four different dietary regimes (enriched rotifers) containing increas...

Evaluation of a new-to-science Artemia population and comparison with four other existing populations were carried out. Five parthenogenetic Artemia populations from the following Greek saltworks were studied: Alyki (a new population), Kalloni, Milos, Polychnitos and Messolongi. The diameters of non-decapsulated and decapsulated cysts as well as th...

This study was conducted to evaluate the accumulation of different levels of total vitamin A in live prey (Brachionus plicatilis and Artemia nauplii) enriched with a commercial emulsion (0.15 and 0.6 g l−1 for rotifers and Artemia nauplii, respectively) , which contained increasing levels of all-trans retinyl acetate. Emulsions used for rotifer enr...

Two fish protein hydrolysates (FPH) were incorporated into four diets prepared for start-feeding sea bass larvae, at two different levels (10% and 19% of total ingredients): a commercial FPH, CPSP, in which the molecular mass of the main fraction of soluble peptides (51%) was between 500–2500 Da, and an experimental FPH obtained by acidic silage of...

The study demonstrates the presence of a nodavirus that affected sturgeon in fresh water, causing disease with neurological signs. The virus was isolated and inoculated onto SSN-1 (striped snakehead, Channa striatus) cell cultures where cytopathic effects (CPE) of the virus included vacuolation of the cells and degeneration of the monolayer. A 255...

We examined flumequine depletion from muscle plus skin of gilthead seabream held in seawater at 18 and 24 °C. Seven groups of 10 fish each were sampled at intervals ranging from 24 to 168 h after in-feed administration of flumequine at 35 mg/kg/day for 5 days. Muscle plus skin tissue samples were analyzed for flumequine by high-performance liquid c...

Fish processing creates a large amount of waste of high nutrient content which, if not properly processed for use in human or animal nutrition, is likely to be deposited in the environment creating pollution problems. Waste parts from rainbow trout processing for smoking, consisting of heads, bones, tails and intestines, were used as feed ingredien...