Yanfei Wang

University of Delaware | UDel UD · School of Marine Science and Policy

Ammonium and polyamines are essential nitrogen metabolites in all living organisms. Crosstalk between ammonium and polyamines through their metabolic pathways has been demonstrated in plants and animals, while no research has been directed to explore this relationship in algae or to investigate the underlying molecular mechanisms. Previous research...

Nitrate reductase (NR) catalyzes the rate-limiting step in nitrate assimilation. Plant and algal NRs have a highly conserved domain architecture but differ in regulation. In plants, NR activity is regulated by reversible phosphorylation and subsequent binding of 14-3-3 proteins at a conserved serine residue. Algal NRs typically lack 14-3-3 binding...

Dinoflagellates are among the most toxigenic phytoplankton that cause harmful algal blooms; they can produce toxins that accumulate through the aquatic food chains to cause illness and even death in marine animals and humans. Shewanella sp. IRI-160 is a naturally-occurring bacterium that secretes a suite of algicidal compounds (collectively designa...

Shewanella sp. IRI-160 is an algicidal bacterium that secretes an algicide, IRI-160AA. This algicide specifically targets dinoflagellates, while having no adverse effects on other algal species tested. Dinoflagellates exposed to IRI-160AA exhibited increased production of reactive oxygen species (ROS), DNA damage, and cell cycle arrest, implying a...

Interactions between bacteria and phytoplankton in aqueous ecosystems are both complex and dynamic, with associations that range from mutualism to parasitism. This review focuses on algicidal interactions, in which bacteria are capable of controlling algal growth through physical association or the production of algicidal compounds. While there is...

This chapter presents an overview of quantitative real-time PCR (qPCR), with a focus on harmful algae blooms (HABs). It includes an overview of the technology and describes the early development of qPCR assays to detect cryptic species. A review of multispecies and ecotype- or strain-level detection approaches by qPCR is provided. This is followed...

Shewanella sp. IRI-160 is an algicidal bacterium isolated from Delaware Inland Bays. It secretes water-soluble compounds that inhibit the growth of dinoflagellates. Previous research indicated that this bacterium does not have a negative impact on other algal species. In this research, Shewanella sp. IRI-160 was immobilized to different porous matr...

Due to increasing evidence of key chemically mediated interactions in marine ecosystems, a real interest in the characterization of the metabolites involved in such intra and interspecific interactions has emerged over the past decade. Nevertheless, only a small number of studies have succeeded in identifying the chemical structure of compounds of...

Eukaryotic nitrate reductase (NR) catalyzes the first step in nitrate assimilation and is regulated transcriptionally in response to external cues and intracellular metabolic status. NRs are also regulated post-translationally in plants by phosphorylation and binding of 14-3-3 proteins at conserved serine residues. 14-3-3 binding motifs have not pr...

Molecular methods such as quantitative real-time PCR (qPCR) and sandwich hybridization assay (SHA) enable a more rapid and specific enumeration of harmful algal species compared to microscopic cell counts. Integrating these methods into routine monitoring and management strategies, however, has been slow. While comparisons to microscopy have been m...

Three important harmful algal bloom causative species, Prymnesium parvum( strain number: JX12), Karlodinium veneficum( strain number: JX24), and Akashiwo sanguinea( strain number: JX14) were isolated and identified from Pearl River Estuary. The effect of different temperature and pH value on the hemolytic activity of P. parvum were investigated und...