Yan Wang

• PostDoc Position at Zhejiang University

Listeriosis, caused by Listeria monocytogenes (Lm), is a severe foodborne illness with a high fatality rate. Listeria phages specifically target and lyse Lm, offer a promising alternative for biocontrol and phage therapy. However, most existing studies focus on the lytic characteristics of Listeria phages using limited sample sizes. In this study,...

Key message Acting as a nucleus-localized transcriptional activator, RsWRKY75 promotes ROS scavenging and Cd efflux by activating the transcription of RsAPX1 and RsPDR8 in radish. Abstract Radish (Raphanus sativus L.) is an important economical root vegetable crop worldwide. As a toxic heavy metal, cadmium (Cd) can dramatically hamper radish tapro...

Background Currently, most studies only focus on the glucose management level and self- management behavior of pregnant women with gestational diabetes mellitus, but lack analysis and discussion on their decision-making behavior and influencing factors during glucose management. Aim This study aimed to investigate the decision-making behavior of b...

Preservation of soil organic carbon (SOC) of forestlands has been challenged with land degradation and climate warming. However, the impact of lithology on SOC preservation in karst forestlands has been poorly addressed. Topsoil core samples of forestlands were collected from karst terrains in central Guizhou, Southwest China, over sandstone (SS),...

Objective The aim of this study was to establish an ensemble learning model based on clinicopathological parameter and ultrasound radomics for assessing the risk of lateral cervical lymph node with short diameter less than 8 mm (small lymph nodes were used instead) metastasis in patients with papillary thyroid cancer (PTC), thereby guiding the sele...

The organic and inorganic composition of pyrolytic liquids derived from biomass pyrolysis and their effects on plant growth with foliar fertilization are insufficiently understood. Particularly, whether there is a link between the composition and effect on plant growth and their property of feedstock and of pyrolysis conditions remains unknown. In...

Background This study evaluated the effect of microvesicles(MVs) from quiescent and TGF-β1 stimulated hepatic stellate cells (HSC-MVs, TGF-β1HSC-MVs) on H2O2-induced human umbilical vein endothelial cells (HUVECs) injury and CCl4-induced rat hepatic vascular injury. Methods HUVECs were exposed to hydrogen peroxide (H2O2) to establish a model for v...

Streptococcus suis is a significant and emerging zoonotic pathogen. ST1 and ST7 strains are the primary agents responsible for S. suis human infections in China, including the Guangxi Zhuang Autonomous Region (GX). To enhance our understanding of S. suis ST1 population characteristics, we conducted an investigation into the phylogenetic structure,...

Objectives: The purpose of this research is to summarize the structure of radiomics-based knowledge and to explore potential trends and priorities by using bibliometric analysis. Methods: Select radiomics-related publications from 2012 to October 2022 from the Science Core Collection Web site. Use VOSviewer (version 1.6.18), CiteSpace (version 6.1....

Background The reporting and data system (RADS) has been researched across the world since it was first developed. This study used bibliometrics to analyze the research trends and current status of this field over the past almost 23 years and explored possible future research hotspots. Methods We searched the Web of Science (WOS) literature on RAD...

Electrochemical oxidation combined with reagents of O3, H2O2 and FeCl2 was conducted in this study to treat the wastewater from wet fermentation of Yunnan arabica coffee. In addition, the effect of oxidants on the efficiency of wastewater treatment, the binding capacities of the oxidants to proteins, the degradation of organic pollutants in the was...

Soil amendment with biochar is being promoted as a promising strategy for carbon (C) stabilization and accrual, which are key to climate change mitigation. However, it remains elusive on how biochar addition influences nonbiochar C in soils and its mechanisms, especially in the presence of plants. Here we conducted a 365-day soil microcosm experime...

Massive amount of food waste has been generated annually, posing a threat to ecological sustainability and the social economy due to current disposal methods. Urgent action is needed worldwide to convert the traditional pathway for treating food waste into a sustainable bioeconomy, as this will significantly benefit food chain management. This stud...

Introduction Listeria monocytogenes is a globally distributed bacterium that exhibits genetic diversity and trait heterogeneity. The alternative sigma factor SigB serves as a crucial transcriptional regulator essential for responding to environmental stress conditions and facilitating host infection. Method We employed a comprehensive genetic anal...

The mechanisms of rape straw biochar that affect the fixation of cadmium (Cd) in paddy soil by influencing redox of iron and sulfur are unclear. Several anaerobic incubation experiments were carried out using Cd-contaminated paddy soils (LY and ZZ). Rape straw biochar at pyrolysis temperatures of 450 °C (LRSB) and 800 °C (HRSB) was selected as the...

Background COVID-19 caused mild to severe infections in humans. The long-term epidemic environment harms people’s mental health. To explore the impact of the epidemic on people’s mental and psychological conditions, we surveyed in Wenzhou. Methods We collected the data of people who visited the First Affiliated Hospital of Wenzhou Medical Universi...

Listeria monocytogenes is an important pathogen that can cause listeriosis. Despite the growing recognition of Listeria spp. as a foodborne and environmental pathogen, the understanding of its prevalence and characteristics of Listeria spp. in the marine environment remains unknown. In this study, we first investigated the genetic and phenotypic ch...

Background and purpose: The relationship between blood urea nitrogen (BUN) and other markers of renal function and the recurrence of acute ischemic stroke (AIS) have been scarcely reported. Inflammatory mechanisms may play a role in these relationships.The aims of this study were to investigate the relationship between BUN and recurrence of AIS and...

Cd is normally associated with sulfide and Fe oxides in flooded paddy soil. The mechanisms of biochar enhanced Cd immobilization by promoting Fe transformation and sulfide formation are unclear. Rape straw biochar (RSB) pyrolyzed at 450 °C (LB) and 800 °C (HB) was added to Cd-contaminated paddy soil at 1% (LB1, HB1) and 2% (LB2, HB2) doses. The res...

The status of soil microbiome has become global concern for earth sustainability. The pool size of microbial biomass carbon (MBC) provides a basic tool to generally assess the soil microbes related to soil health in global terrestrial ecosystems. Albeit, the variation of topsoil MBC pool remains largely uncertain with land use patterns across China...

SWEET (Sugars Will Eventually be Exported Transporter) genes play essential roles in various biological processes, including phloem loading, sugar efflux, plant development and stress response. In this study, a total of 33 RsSWEET gene members were identified in the radish genome. They could be divided into four subfamilies and are distributed on e...

Radish (Raphanus sativus L.) is an economically important and widely cultivated root vegetable crop. The coloration of the green skin and green flesh is an important trait influencing the nutrition and flavor quality in fruit radish. GOLDEN2-LIKEs (GLKs) play critically important roles in plastid development and chlorophyll biosynthesis in plants....

Listeria monocytogenes is a major human foodborne pathogen and a ubiquitous environmental saprophyte. In this study, we investigated the prevalence and characteristics of Listeria spp. from beach sand in the coastal environment. Three different Listeria spp., Listeria monocytogenes (n=16), Listeria fleishmanii (n=7), and Listeria aquatica (n=3) wer...

Radish is an economical cool-season root vegetable crop worldwide. Heat shock protein 70 (HSP70) plays indispensable roles in plant growth, development and abiotic stress responses. Nevertheless, little information is available regarding the identification and functional characterization of HSP70 gene family in radish. Herein, a total of 34 RsHSP70...

Co-pyrolysis is a potentially effective method for both biomass waste management and multi-functional biochar-based product design. It involves the thermochemical decomposition of biomass waste under anoxic conditions, which can reduce the cost of disposal and produce biochar with beneficial properties. Herein, this study aimed to investigate the p...

The growth and development of taproots are inhibited by cold stress in radish (Raphanus sativus L.). Ethylene-responsive element binding factors (ERF) are key participators in the cold stress response and growth regulation of plants. However, the functions of ERF genes in cold tolerance and root development in radish remains elusive. Here, we showe...

Introduction Listeria monocytogenes is a foodborne bacterium that could persist in food and food processing environments for a long time. Understanding the population structure and genomic characterization of foodborne L. monocytogenes is essential for the prevention and control of listeriosis. Methods A total of 322 foodborne L. monocytogenes iso...

To further investigate the effect of pyrolysis temperature on bioavailable Cd, Pb and As, as well as the bacterial community structure in multi-metal(loid) contaminated paddy soil, six types of biochar derived from wood sawdust and peanut shell at 300 °C, 500 °C and 700 °C were prepared and incubated with Cd, Pb and As contaminated paddy soil for 4...

In most higher plants, sucrose is the significant form of carbohydrate for long-distance transportation. Sucrose transporters/sucrose carriers (SUTs/SUCs) are involved in the loading and unloading of sucrose in phloem and play an important role in the growth and development of plants. In this study, 12 RsSUC genes were first identified from the rad...

Listeria monocytogenes is a ubiquitous foodborne pathogen causing both invasive and non-invasive listeriosis. Sequence type (ST) 9 strains is common in food and food processing environments. In this study, the whole-genome sequences (WGS) of 207 ST9 isolates from different sources, geographical locations (14 countries), and isolated years were anal...

The properties of biochar determine its ability to alleviate soil acidity and improve plant growth; however, its key properties remain unclear. Therefore, the purpose of this study was to screen the key characteristics of biochar that strongly influence soil acidity and plant growth to obtain a better estimation of biochar characteristics that is s...

Background:COVID-19 caused mild to severe infections in humans. The long-term epidemic environment harms people's mental health. To explore the impact of the epidemic on people's mental and psychological conditions, we surveyed in Wenzhou. Methods: We collected the data of people who visited the First Affiliated Hospital of Wenzhou Medical Universi...

Background The treatment and prevention of perinatal venous thromboembolism (VTE) are challenging because of the potential for both fetal and maternal complications. Methods This study developed a rapid assessment scale for VTE and evaluate its validity based on Delphi-AHP (Analytic Hierarchy Process) method in China. The research was conducted by...

Contamination of food by Listeria monocytogenes at retail level leads to potential consumption of contaminated food with high risk of human infection. Our previous study found persistent contamination of CC87 L. monocytogenes from a retail market in China through pulsed-field gel electrophoresis and multilocus sequence typing.

Reduced nitrogen (N) and phosphorus (P) released from sediments in rural areas pose a significant challenge to residential sewage treatment and management. Under different disturbance velocities, simulation experiments were undertaken to investigate the effect of disturbance on the release of N and P from rural ditch sediments into the water at the...

Background Serum uric acid (UA) has been reported to be associated with ischemic stroke and inflammation. However, whether or not UA is related to the recurrence of ischemic stroke, and whether inflammation plays a role in the relationship between them remain inconclusive. Objective We sought to explore the relationship between UA and the recurren...

Worldwide, massive amounts of food waste have been generated at increasingly accelerated rates, posing potentially great risks to the environment. Shifting traditional waste treatment to a sustainable bioeconomy thus challenges food chain management worldwide. In this study, food waste was copyrolyzed with rice husk to produce biochar with a pilot-...

Fast accumulation of soil organic matter (SOM) following forest restoration shifted from cropland has been widely reported, but how the pools and molecular composition changed across soil aggregate fractions remains unclear. In this study, undisturbed topsoil (0–10 cm) samples were collected across a decadal chronosequence of forest stands (RL10, R...

Cadmium (Cd) and Arsenic (As) in rice grains are a primary exposure source for human beings. However, the simultaneous stabilization of Cd and As in soil becomes difficult due to the opposite properties of those. In this study, we investigated the simultaneous effects of biochar-supported nanoscale zero-valent iron (nZVI-BC) and water management on...

This study aimed to investigate the prevalence of fosfomycin fosA7 in Salmonella enterica isolates from food animals and retail meat products in China and the impact of fosA7 on bacterial fitness. A total of 360 Salmonella isolates collected from 11 provinces and cities in China were detected for fosA7 . All fosA7 -positive Salmonella isolates were...

We isolated eight tigecycline-resistant Enterobacteriaceae strains from a pig farm in Shanghai, China, including Escherichia coli (n = 1), Proteus cibarius (n = 1), and Enterobacter hormaechei (n = 6). Two of them (E. coli and P. cibarius) were positive for tet(X). E. coli SH19PTE6 contained an IncFIA18/IncFIB(K)/IncX1 hybrid plasmid pYUSHP6-tetX,...

Recently, Streptococcus suis reference strains of serotype 20, 22, and 26 were reclassified as Streptococcus parasuis. The public health significance of S. parasuis is underestimated due to the lack of clinical isolates. In the present study, we first reported two sporadic S. parasuis infections in humans, after using full-length 16S rRNA and house...

The sorption capacities of the macrofungus viz. Ganoderma lobatum (C0) and its biochar (C400) were evaluated for the biosorption of Cu(II) from aqueous solution under different conditions, including adsorbent doses, pH of the solution, contact time and initial Cu(II) concentration. The results showed that Ganoderma lobatum could be used as an effic...

Aim: To identify a set of scientific, systematic and clinically applicable nursing-sensitive quality indicators for pernicious placenta previa (PPP). Design: A modified Delphi-Consensus Technique. Methods: According to literature retrieval published between 2009-2019, 38 nursing-sensitive quality indicators were chosen and a questionnaire was...

Listeria monocytogenes, a fatal foodborne pathogen has the extraordinary capacity to survive in harsh conditions and is a potential threat to public health. A novel 91kb plasmid pLM1686 was found in the prevalent L. monocytogenes sequence type (ST) 87 strain in China. In this study, the function and distribution of pLM1686 were firstly investigated...

Tetrahydropalmatine (dl-THP) demonstrates an analgesic effect in animal models of neuropathic and inflammatory pain, however, the underlying mechanisms of its pharmacological action within the spinal cord remains unclear. Both P2X3 receptor and TRPV1 are associated with the development and progression of such neuropathic and inflammatory pain. Here...

Background and Objectives To compare the efficacy and safety of 1064‐nm and dual‐wavelength (532/1064 nm) picosecond Nd:YAG lasers with a holographic optic in the treatment of facial photoaging. Study Design/Materials and Methods In this prospective, randomized split‐face study, each half of each participant′s face was randomly treated with a 1064...

Heavy-metal contamination is widespread in agricultural soils worldwide, especially paddy soils contaminated by Cd. Amendment-induced immobilization of heavy metals is an attractive and effective technique, provided that cost-effective materials are used. This field experiment compared three alkaline passivators (attapulgite, processed oyster shell...

Both spinophilin (SPN, also known as neurabin 2) and Rac1 (a member of Rho GTPase family) are believed to play key roles in dendritic spine (DS) remodeling and spinal nociception. However, how SPN interacts with Rac1 in the above process is unknown. Here, we first demonstrated natural existence of SPN-protein phosphatase 1-Rac1 complex in the spina...

Great progress has been made in recent years to alleviate the heavy metal pollution, but the development of low-cost and eco-friendly adsorbents is still challenging. In this work, MgO-loaded biochar, as a potential adsorbent, was successfully synthesized via copyrolysis of corn straw and MgCl2∙6H2O at 600 ℃ and used for heavy metal immobilization...

This study aimed to characterize 16S rRNA methylase genes among Salmonella and to elucidate the structure and evolution of rmtB-carrying plasmids. One hundred fifty-eight Salmonella isolates from one pig slaughterhouse were detected as containing 16S rRNA methylase genes; two (1.27%) Salmonella London isolates from slaughtered pigs were identified...

Pullorum is a chicken–specific systemic disease caused by Salmonella enterica serovar Gallinarum biovar Pullorum (S. Pullorum). This study was carried out to provide basic data for understanding the trends of S. Pullorum. A total of 652 S. Pullorum isolates collected in China during 1962–2019 were examined. Overall, 525 (80.5%) isolates were resist...

The occurrence and characterization of carbapenemase-producing Enterobacteriaceae from companion animals in Guangzhou, China, are investigated. Six isolates (2.3%, 6/257) were positive for blaNDM-5, that is, one Enterobacter cloacae, one Citrobacter freundii, and four Escherichia coli. Three E. coli isolates obtained from the same animal hospital w...

The purpose of this study was to characterize the complete sequence of a novel plasmid carrying tigecycline resistance gene tet(X) and carbapenemase gene blaOXA-58 from a swine Acinetobacter sp. strain SH19PTT10. Minimal inhibitory concentration (MIC) was performed using microbroth dilution method. The isolate SH19PTT10 was highly resistant (16 mg/...

Senecavirus A (SVA), a recently emerging picornavirus, poses a great threat to the swine industry because it causes swine idiopathic vesicular disease and epidemic transient neonatal losses. Thus far, the progress in SVA viral pathogenesis studies and vaccine development remains sluggish, and an available and convenient reverse genetics system woul...

The use of degradable polymer materials as controlling additives to the working fluid during hydraulic fracturing is an area of interest in developing stimulation technologies. A temporary plugging agent with high compressive strength is used to block pre-fracturing or natural cracking and to initiate new fracture joints in order to increase the oi...

Due to the decline of formation pressure in the middle and late periods of low permeability reservoir development, the development effect deteriorates. Therefore, it is essential to restore and maintain reasonable formation pressure. Based on the two-phase fractional flow equation in low permeability porous media, we consider the flow characteristi...

Contaminations by heavy metals in the environment always exist as a mixture of both metal and metalloid. Thus, it is a challenge to simultaneously remove both components due to their adverse chemical behaviors. Herein, effective cadmium (Cd) and arsenic (As) removal in aqueous solution was achieved by use of a novel composite, which was synthesized...

Salmonella Indiana has emerged in recent years as an important zoonotic pathogen, but its pathogenicity has not been fully elucidated. In this study, using in vivo and in vitro animal and cellular experimental model systems, we evaluated the pathogenicity of Salmonella Indiana (S. Indiana) compared with three other serotypes of Salmonella, S. Enter...

Using a rat model of trigeminal neuropathic pain (TNP) produced by chronic compression of the infraorbital nerve (CCI-ION), we investigated the analgesic effect and the underlying mechanisms of ceftriaxone (Cef), a β-lactam antibiotic, that is thought to be a potent stimulator of glutamate transporter 1 (GLT-1). First, repeated intraperitoneal (i.p...

Electricity cost is one of main production costs for flow shops. Providing frequency regulation services can help electric loads reduce their electricity costs. Previous studies mostly focus on automatic generation control (AGC) strategies for other types of electric loads, such as air conditioners, EVs or battery storage. In this paper, we find fl...

Variations of phytoaccumulation and tolerance in different growth stages of plant are important factors for effective removal of pollutants in phytoremediation. The present work investigated arsenic (As) accumulation, As-tolerance and the physiological tolerance mechanisms of Typha angustifolia under different As-level during the seedling, fast-gro...

Listeria ivanovii subsp. ivanovii is an intracellular bacterium distributed widely in nature, causing the listeriosis in ruminants and humans. Previous researches had isolated 116 strains of L. ivanovii subsp. ivanovii from wild rodents and pikas of different regions in China, and the predominant sequence types were ST1 and ST2. In this study, we f...

Infectious bronchitis virus (IBV), an ongoing emergence enveloped virus with a single‐stranded positive‐sense RNA genome, belongs to the Gammacoronavirus genus in the Coronaviridae family. IBV‐associated tracheitis, nephritis, salpingitis, proventriculitis and egg drop have caused devastating economic losses to poultry industry worldwide. Since the...

Background: Listeria monocytogenes consists of four lineages that occupy a wide variety of ecological niches. Sequence type (ST) 87 (serotype 1/2b), belonging to lineage I, is one of the most common STs isolated from food products, food associated environments and sporadic listeriosis in China. Here, we performed a comparative genomic analysis of...

Background: Neonatal listeriosis is a rare but severe disease manifesting as septicemia and central nervous system (CNS) infections with a high fatality rate of around 20 to 30%. Whole genome sequencing (WGS) is a promising technique for pathogen identification and infection source tracing with its high resolution. Case presentation: A case of n...