Yan Li

Sun Yat-Sen University | SYSU

Diabetic wounds (DWs) are a common complication of diabetes mellitus; DWs have a low cure rate and likely recurrence, thus affecting the quality of patients' lives. As traditional therapy cannot effectively improve DW closure, DW has become a severe clinical medical problem worldwide. Unlike routine wound healing, DW is difficult to heal because of...

The delayed and complicated diabetic wound healing raises clinical and social concerns. The application of stem cells along with hydrogels is an attractive therapeutic approach. However, low cell retention and integration hindered the performance. Herein, gelatin microspheres were fabricated for local delivery of adipose-derived stem cells (from ra...

The scaffold microstructure is important for bone tissue engineering. Failure to synergistically imitate the hierarchical microstructure of the components of bone, such as an osteon with concentric multilayers assembled by nanofibers, hinders the performance for guiding bone regeneration. Here, a 2D bilayer nanofibrous membrane (BLM) containing pol...

Imaging-guided vascular embolization is frequently performed on patients with advanced hepatocellular carcinoma (HCC) to alleviate symptoms and extend their survival time. However, current operation procedures were not only painful for patients, but also resulted in inaccuracy in tumor targeting after the release of embolic agents from the catheter...

Injury of corpus cavernosa results in erectile dysfunction, but its treatment has been very difficult. Here we construct heparin-coated 3D-printed hydrogel scaffolds seeded with hypoxia inducible factor-1α (HIF-1α)-mutated muscle-derived stem cells (MDSCs) to develop bioengineered vascularized corpora. HIF-1α-mutated MDSCs significantly secrete var...

An anti-EGFR nanobody was labeled at the C-terminus with a lysosome-sorting NPGY (Asn-Pro-Gly-Tyr) motif via sortase-mediated ligation to enhance the engagement of the clathrin-mediated endocytosis. The synergistic effects of NPGY motif and nona-arginine peptide were found to induce robust internalization and lysosomal trafficking, which in turn im...

Limited dimensional stability and osteogenic induction property of poly (lactide-co-glycolide) (PLGA) electrospun membranes hampered their applications in bone tissue engineering. Thermal treatment of fixed PLGA membranes at 50 °C for 2 h and further immersion in 75% ethanol at free-standing state were adopted in order to obtain a high stability an...

Injectable scaffolds have attracted much attention because of their minimum surgical invasiveness. However, limited osteogenic induction property and low mechanical properties hampered their application in bone tissue engineering. CaCO3 microspheres, which possess osteoinductivity, rough surfaces and specific binding sites for BMP-2, were first fab...

Hydrogel-based wound dressings provided a moist microenvironment and local release of bioactive molecules. Single drug loading along with fast release rates and usually in hydrogel sheets limited their performance. Hence, uniform Alginate/CaCO3 composite microparticles (~430 μm) with tunable compositions for sustainable release of drug and pH-sensi...

In this work, we present a novel class of uniform eccentric magnetic microcapsules based on polydimethylsiloxane (PDMS) for magnetic resonance imaging (MRI)-guided local injection and pH-regulated drug release. The microcapsules contained magnetic nanoparticles in their PDMS shells, which allowed them to be easily tracked by MRI during administrati...

Front Cover: In article 1800130 by Ling Yan, Yan Li, and co‐workers, electrospun composite fibers in multi‐scale structures are formed, which contain microparticles with both hydroxyapatite (HAp) and CaCO3, and also casein, in a hierarchical structure. Random composite fibers significantly enhance proliferation and osteogenic differentiation of adi...

Ultrasound controlled drug delivery and release has attracted increased attention for targeted delivery of drug. In this report, we present a strategy for targeted drug delivery by using ultrasound to image the location of drug carriers, as well as simultaneously control the release rate of drug from elastic eccentric microcapsules (EEMs), based on...

A novel approach, continuous needleless electrospinning from the tips of magnetization-induced self-assembling PVA/ferrofluid cone array, was proposed to prepare magnetic nanofibers. A PVA/ferrofluid was synthesized, the needleless electrospinning process was observed, and the morphology and magnetic properties of magnetic nanofibers were investiga...

Graphene and its derivatives, along with adipose-derived stem cells (ADSCs), have demonstrated great potential to be used in neuron tissue engineering. ADSCs from rats (rADSCs) were first isolated and the surface markers were evaluated using flow cytometry. The rADSCs were fibroblast-like, positively expressed CD90 and CD105, lacked surface markers...

Uniform Casein/CaCO3 microspheres were fabricated with well tuned properties and the formation mechanism based on the templating effect of casein micelles was proposed. In bone tissue engineering, vaterite microspheres were promising because of the biocompatibility and being easily fabricated. Here Casein/CaCO3 microspheres were precipitated by mix...

Poly(lactide-co-glycolide) (PLGA) electrospun composite nanofibers were fabricated to mimic bone extra cellular matrix for applications in bone tissue engineering. Poly(acrylic acid) modified Zn-doped calcium phosphate (PAA-CaP/Zn) nanoparticles were first synthesized. PAA was selected because of the strong bonding ability with calcium and Zn was i...

Lung cancer bone metastases usually involve multiple interactions between cancer cells and host organs. To mimic such a structure, a cell co-culture micropattern of A549 lung cancer cells and osteoblast cells (A549/OB) was developed using a μ-eraser strategy for anti-cancer drug evaluation and also understanding cell–cell communication. When applyi...

Biomimetic scaffold is promising for repairing bone defects that are resulted from diseases or injuries. An ideal scaffold should be similar to native bones. Thus, hydroxyapatite (HAp) composite microparticles in which HAp closely resembles the mineral component in native bones were fabricated. Casein/CaCO3 microspheres, precipitated in the presenc...

Worker honeybee pierces animal or human skin with its ultra-sharp stinger and injects venom to defend itself. The insertion behavior is a painless transdermal drug delivery process. In this study, Apis cerana cerana worker honeybee was chosen as the research object. The geometry and structure of the stinger were observed by the Scanning Electron Mi...

Creating a bioactive surface is important in tissue engineering. Inspired by the natural calcium binding property of casein (CA), multilayer films ((CA/CS)n) with chitosan (CS) as polycation were fabricated to enhance biomineralization, cell adhesion and differentiation. LBL self-assembly technique was used and the assembly process was intensively...

Tissue engineering offers a promising approach to repair bone defects, of which scaffold is an indispensible component. An ideal scaffold should mimic the organic and inorganic compositions of bone. Here, CaCO3/casein microspheres were encapsulated in PCL composite membranes using co-solvent electrospinning to mimic the architecture and composition...

For tissue engineering applications, it is important to develop fabrication strategies for building models with controlled cell distributions in defined structures. Here, a simple, flexible approach (named the μ-eraser strategy) is developed to construct multicell micropatterns. This approach involves pressing a poly(dimethylsiloxane) stamp to eras...

In this paper, we fabricated uniform polydimethylsiloxane (PDMS) magnetic microcapsule with eccentric internal structure, and employed it as a novel delivery system for orientation-specific and dual stimuli-responsive controlled drug release. These eccentric microcapsules contained Fe3O4 nanoparticles in the inner cores. Because of the paramagnetic...

In this paper, a strategy was developed for fabricating uniform polydimethylsiloxane (PDMS) microcapsules with eccentric and core-centered internal hollow structures, which can be employed as a novel controlled-release system for site-specific drug delivery under ultrasound regulation. This strategy involved the use of a microfluidic device, throug...

A novel porous crack composite wick flattened heat pipe (PCHP) has been developed for electronic device cooling. PCHP was fabricated from grooved–sintered wick cylindrical heat pipe (GSHP) by phase change flattening technology. The composite wick was composed of porous sintered powder structure and axial micro-crack channels. The crack channels of...

Miniature cylindrical metal powder sintered wick heat pipe (sintered heat pipe) is an ideal component with super-high thermal efficiency for high heat flux electronics cooling. The sintering process for sintered wick is important for its quality. The sintering process was optimally designed based on the equation of the heat transfer limit of sinter...

Cartilage repair by mesenchymal stem cells (MSCs) often occurs in diseased joints in which the inflamed microenvironment impairs chondrogenic maturation and causes neo-cartilage degradation. In this environment, melatonin exerts an antioxidant effect by scavenging free radicals. This study aimed to investigate the anti-inflammatory and chondroprote...

5-Fluorouracil (5FU) was successfully entrapped within poly(lactide-co-glycolide) (PLGA) and hydroyapatite (HA) composite microspheres using the emulsification/solvent extraction technique. The effects of HA to PLGA ratio, solvent ratio as well as polymer inherent viscosity (IV) on encapsulation efficiency were investigated. The degradation and dru...

To reduce the toxicity and achieve a sustainable and controllable release of cisplatin (CDDP). CDDP was loaded onto Fe5 (Fe(3+) doped hydroxyapatite at atomic ratio of Fe(added)/Ca(added) = 5%) nanoparticles through surface adsorption. Subsequently, CDDP-loaded Fe5 nanoparticles (CDDP-Fe5) and/or CDDP were encapsulated into poly(lactide-co-glycolid...

Manganese (II) and iron (III) substituted hydroxyapatite (HA, Ca10(PO4)6(OH)2) nanoparticles were synthesized using wet chemical method. All samples were single-phase, non-stoichiometric and B-type carbonated hydroxyapatite. Compared with pure HA, Mn2+ substituted (MnHA) and Fe3+ doped HA (FeHA) did not demonstrate significant structure deviation....

Cu2+ and Ti4+ substituted hydroxyapatite (CuHA; TiHA) in different molar ratios (Madded/Caadded = 0.01, 0.05, 0.10 and 0.15) were synthesized using wet chemical method coupled with ion exchange reaction. All characterization results confirmed the samples were hydroxyapatite (HA). XRD data showed that all samples were single phased. Both intensity a...

Calcium hydroxyapatite (HA) is the main inorganic component of natural bones and can bond to bone directly in vivo. Thus HA is widely used as coating material on bone implants due to its good osteoconductivity and osteoinductivity. Metal ions doped HA have been used as catalyst or absorbents since the ion exchange method has introduced new properti...

Nanocrystalline hydroxyapatite (HA), Ca10(PO4)6(OH)2, substituted with gadolinium(III) (Gd3+) and neodymium(III) (Nd3+) in different mole ratios (Madded/Caadded=XM, 0.01, 0.05, 0.1, and 0.2), was synthesized by an ion-exchange method. All samples had the single phase of HA except for XM=0.2, which displayed the second phase of M(OH)3. All were B-ty...