Yame Fabres Robaina Sancler-Silva

Universidade Federal de Viçosa (UFV) | UFV · Department of Animal Science

This study aimed to evaluate the effects of differential average daily gain targets of dairy heifers throughout gestation on placental hemodynamics, uterine involution, colostrum production of the heifers, and impacts on newborn calf weight and immunity transfer. Fourteen Holstein × Gyr heifers with an average body weight of 446 ± 46.7 kg and age o...

Endometritis is a relevant cause of subfertility in mares. However, the accurate diagnosis, essential for effective treatment, can be difficult due to the variability of results and interpretations resulting from different examination methods and sample collection techniques. The present work compared gynecological evaluation methods and sample col...

Evaluations of replacement heifers in intensively managed grazing systems in tropical conditions are warranted. Thus, we aimed to evaluate performance, muscle and mammary gland development, oocyte quality, and in vitro production of embryos of crossbred heifers grazing an intensively managed pasture and supplemented with high or low protein concent...

Seminal vesiculitis in stallions reduces fertility and is often underdiagnosed. The most common cause is infection of seminal vesicles by bacteria capable of forming biofilms and a propensity for tissue persistence, e.g. Pseudomonas aeruginosa. Achieving a clinical cure is challenging, due to a high rate of recurrence. Systemic antibiotic therapy d...

Scrotal heat stress leads to testicular degeneration as a result of hypoxia, hypoglycemia, reactive oxygen species overproduction, molecular changes and apoptosis. Oral treatment with pentoxifylline has been shown to be beneficial in minimizing histological damages in the testis of rats after induction of intermittent hypoxia and local hyperthermia...

RESUMO Avaliou-se o efeito de curvas de congelação nos parâmetros espermáticos e na fertilidade, usando sêmen de alta e baixa congelabilidade. Experimento 1 - utilizou-se sêmen de quatro garanhões resistentes à congelação: grupo 1, palhetas refrigeradas até 5°C e congeladas com curva de -8°C/min; grupos 2 e 3, palhetas refrigeradas até 5°C (0,5°C/m...

RESUMO O objetivo do presente estudo foi avaliar o efeito da adição de plasma seminal de garanhões de alta e baixa fertilidade sobre a congelabilidade e a viabilidade de espermatozoides do ejaculado (EJ) e do epidídimo (EP) de garanhões subférteis. Foram utilizados seis garanhões com histórico de subfertilidade. Após coleta, espermatozoides do ejac...

In chapters 1 and 2, anatomo-physiological revisions in the male and female reproductive apparatus are contained of equines and bovine animals, respectively. Chapters 3 to 5 are focused on methods of manipulating the cycle of bovine, equine and small ruminants. The main reproductive biotechnologies applied in cows, sheep and goats are described in...

Pentoxifylline has been used in the treatment of rats and men with severe testicular abnormalities, improving their sperm quality and fertility; however, the benefits of this drug have been poorly investigated in the stallion. Therefore, our study aimed to evaluate the effect of oral treatment with pentoxifylline on the seminal quality of stallions...

The aim of this report is to describe a new methodology to successfully treat stallions diagnosed with urethral rent. Four stallions of ages ranging from 7 to 12 years (median 9) with hemospermia were admitted for clinical evaluation, breeding soundness examination and urethroscopy for inspection of the urethra and vesicular glands. Once the presen...

This study investigated the binding capacity of equine spermatozoa (both ejaculated and from different epididymal regions) to the oviductal epithelial cells (OECs) culture, before and after cryopreservation, using an oviduct explant assay. Ejaculated and epididymal sperm from caput, corpus, and cauda of 10 stallions were diluted and submitted to fr...

Fertility rates of donkey semen in jennies are lower compared to mares. The aims of this study were to evaluate different sperm cryopreservation methods and insemination strategies to improve the fertility of donkey semen in jennies. Three experiments were performed: (1) the comparison of two freezing methods of donkey semen (conventional method an...

The penis and prepuce of the stallion have a high bacterial load on its surface, forming a natural microbial flora that contaminates the semen during ejaculation. Bacterial growth in semen may cause a decline on sperm quality, viability, and fertility and predisposes the occurrence of endometritis in inseminated mares. Thus, the aim of this study w...

For many years the pregnancy rate of donkey frozen semen presented lower results in donkey jennies; however, a recent study showed an increase in pregnancy rates using frozen semen added to seminal plasma on post-thaw. A hypothesis for this result is the higher uterine inflammation response after breeding when using seminal plasma. The same studies...

Several factors can interfere with sperm cryopreservation resistance, especially the genetic factors and those related to the plasma membrane composition of the sperm and seminal plasma. However, it is still unclear if the same factors that confer freezing resistance will perform the same role during the cooling process. Thus, the aim of this study...

The aim of the present study was to evaluate the effect of cholesterol-loaded cyclodextrin on the quality and fertility of stallion frozen semen. Three ejaculates from each of 4 stallions were used. The semen was diluted (1:1) with a skim milk-based extender (Botu-Semen(TM), Botupharma, Brazil). The samples were divided into 2 groups: control group...

Commercial freeze extenders have different composition and ratio of cryoprotectors; freezing and thawing protocols are different for each extender. The aim of this experiment was to observe the effect of thawing curve in stallion frozen semen with 2 commercial extenders. Two ejaculates from each of 9 stallions of different breeds (Quarter Horses an...

One study showed increased fertilization in IVF when added seminal plasma of bulls with high fertility rates in semen of low fertile. The aim of the present study was to evaluate the difference of addition homologous and heterologous seminal on donkey frozen semen. Five ejaculates from two donkeys were used. Semen was diluted (1:1) with a milk-base...

A recent study showed increase of pregnancy rates in donkey frozen semen with addition of seminal plasma post-thaw. The aim of the present study was to evaluate the effect of addition post-thaw of seminal plasma on donkey frozen semen quality. Five ejaculates from three donkeys were used. Semen was diluted (1:1) with a milk-based extender (Botu-Sem...

There are few studies with donkey semen in the literature. In horses, is related that no difference are in freezing semen using automated systems or Styrofoam box. The aim of the present study was evaluate donkey frozen semen quality in different freezing conditions. Five ejaculates from there donkeys were used. Semen was diluted (1:1) with a milk-...

In fixed time artificial insemination (FTAI) is frozen semen is commonly used, however the use of cooled semen is a practice alternative to reduce costs. The aim of this study was compare the difference in DNA fragmentation and plasma membrane integrity between chilled and frozen semen of bulls. Two ejaculates from 20 bulls were collected. Semen wa...

The aim of this experiment was compare two egg yolk-based (Tris and botu-Bov) and a coconut water-based (ACP-111) extenders to freeze buffalo semen. Four ejaculates of four buffalo bull were collected. Semen was divided in three groups: EY1 group, the semen was diluted with Tris-Yolk extender; EY2 group, the semen was diluted with Botu-Bov TM; CW g...

The recovery of sperm from the epididymal cauda may be the last chance to obtain genetic material when sudden death or serious injuries occur in valuable stallions. However, the lack of technical knowledge regarding the storage and transportation of the epididymis often prevents the preservation of the sperm. Therefore, the aim of this study was to...

Diagnosis and biological behavior of breast cancer of female dog represent one of the biggest challenges facing the Veterinarian in recent years. Due to its exponential growth and the degree of aggressiveness, the exact cause of this tumor is probably multifactorial and it is believed that may suffer influence from environmental factors. Among the...

The process of spermatic division and differentiation (spermatogenesis) occurs with intratesticular temperature lower that the corporal temperature and for that is essential that the testicular thermoregulation mechanism occurs properly. For evaluation of the scrotal surface temperature can be used the infrared thermography or testicular sensors, b...

The cryopreservation of epididymal sperm is important to preserve genetic material from valuable buffalo bulls. This study evaluated the viability of post-thawed sperm samples recovered from the epididymal cauda adding motility inductors. For that, were used epididymides from eight Murrah buffaloes with 18 months of age. Semen samples were submitte...

The process of spermatic division and differentiation (spermatogenesis) occurs with intratesticular temperature lower that the corporal temperature and for that is essential that the testicular thermoregulation mechanism occurs properly. For evaluation of the scrotal surface temperature can be used the infrared thermography or testicular sensors, b...

Valuable genetic material can be preserved by the cryopreservation of epididymal sperm. This study evaluated the viability of pre-freezing and post-thawed sperm samples recovered from the epididymal cauda of buffaloes. Epididymides from eight Murrah buffaloes with 18 months of age were used. Semen samples were diluted in two different freezing exte...

Previous studies in humans and current studies in horses show that the presence of leukocytes in the semen promotes increased reactive oxygen species and proinflammatory cytokines production. These substances cause damage to membrane integrity, mitochondrial function, DNA integrity, and lead to increased expression of apoptosis markers in the sperm...

The development of a reliable technique for freezing epididymal semen would provide a unique opportunity to preserve valuable genetic material from unexpectedly lost stallions. The semen analysis method with the best ability to predict fertility is an examination of the sperm chromatin structure. This test evaluates the susceptibility of spermatozo...

Embryonic stem cells are pluripotent cells, derived from the inner cells mass of blastocyst embryos. They can differentiate into various cell types and also maintain an undifferentiated state for an unlimited period. They express different stem cells markers, like the transcription factor STAT-3, Oct-4, the cell surface markers SSEA-1, SSEA-3, SSEA...

The hydranencephaly in horses is rare, but is described in other species. It is characterized as a process of destruction of the brain lobes that may be caused as a result of intrauterine viral infection or caused by changes in the uterine vasculature. The reports about this disease are scarce and as such, this paper aims at describing a case of hy...

This study was carried out to evaluate the effect of platelet-rich plasma (PRP) on the treatment of tendinopathy induced in the superficial digital flexor tendon (TFDS) of horses, by using histologic evaluation. Six healthy crossbred geldings aged 8 to 15 years (12±3) were used. The TFDS tendinopathy was provoked in both forelimbs, by intratendinou...

O objetivo deste trabalho foi estudar o efeito do plasma rico em plaquetas (PRP) no tratamento da tendinite induzida no tendão do músculo flexor digital superficial (TFDS) de eqüinos mediante avaliação ultra-sonográfica. Para isso foram utilizados seis eqüinos hígidos machos castrados, com idade de 8-15 anos (x=12 anos). A tendinite do TFDS foi pro...

The objective of the study was to evaluate the effect of platelet-rich plasma (PRP) in the treatment of induced tendinitis in the superficial digital flexor tendon (SDFT) of horses through ultrasonographic evaluation. Thus, six 8 to15-year-old healthy gelding horses (x=12 years) were used. Tendinitis of the SDFT was provoked in both forelimbs via i...