Yakiv Ratushnyak

Yakiv Ratushnyak
Institute of Cell Biology and Genetic Engineering · Laboratory of Adaptive Biotechnology, Department of genetic engineering

Doctor of Philosophy

About

28
Publications
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129
Citations

Publications

Publications (28)
Conference Paper
Full-text available
The ever-growing needs of modern science and technology in new materials with specific properties increase the interest of the world industrial and scientific community in nanomaterials. The magnetic nanomaterials have attracted perspective in biomedical and environmental applications because of their effective and low toxicity for human cells. Thi...
Article
Full-text available
The aim of the study was to evaluate the long-term effect of Agrobacterium rhizogenes genes transfer on plant antioxidant system by the study of superoxide dismutase (SOD) activity in “hairy” roots of Artemisia and Althaea spp plants. PCR analyses revealed stability of the transformation and presence of bacterial rol B and rol C genes in the “hairy...
Article
Full-text available
The “green” synthesis of magnetite and cobalt ferrite nanoparticles (Fe 3 O 4 -NPs and CoFe 2 O 4 -NPs) using extracts of Artemisia annua L “hairy” roots was proposed.
Article
Full-text available
Transformation using Agrobacterium rhizogenes is a widely used method for obtaining "hairy" roots of different plant species. Such roots are characterized by rapid growth under simple growing conditions. Incorporation of bacterial rol genes into the plant genome after the transformation can lead to changes in bioactive compounds synthesis in "hairy...
Article
Full-text available
The research was focused on the synthesis of silver nanoparticles (AgNPs) using extracts from the “hairy” root cultures of Artemisia tilesii Ledeb. and Artemisia annua L. The effect of operational parameters such as type of solvent, temperature of extraction, flavonoids concentration, and reducing power of the wormwood “hairy” root extracts on the...
Article
Plants belonging to the genus Artemisia L. have been used for medicinal purposes since ancient times. These aromatic plants produce and accumulate a wide range of potent secondary metabolites, many of which have shown antioxidant, antiparasitic, antimicrobial, anti-inflammatory, and even anticancer activities. Enhanced biosynthesis of these compoun...
Conference Paper
Full-text available
Plant-mediated “green” synthesis of compounds with medicinal properties attracts great interest for many years. “Hairy” root cultures can synthesize numerous compounds including phenols, flavonoids, essential oils, sugars etc. The possibility to increase the amount of these compounds due to transfer of rol genes from Agrobacterium rhizogenes was co...
Article
We investigated the effect of Agrobacterium rhizogenes-mediated transformation on antioxidant activity of Artemisia vulgaris “hairy” roots. It appeared that transformation may increase flavonoid content as well as DPPH-scavenging activity and ability to reduce Fe³⁺ as compared to the non-transformed plants. Some “hairy” roots accumulated flavonoids...
Article
The effect of A. rhizogenes-mediated genetic transformation on the antioxidant status of Artemisia tilesii, A. vulgaris, A. dracunculus, and A. annua transgenic roots has been studied. Antioxidant activity (AOA) of aqueous extracts was determined using methods based on the ability to reduce DPPH⁺ and ABTS⁺-radicals. The level of AOA (DPPH) in 50% o...
Article
Full-text available
It was carried out a comparative assessment of polifructan content in transgenic roots and control plants Artemisia annua, A. tilesii, A. dracunculus, A. ludoviciana, A. absinthium. For this purpose two simple methods of data visualization were used. Variability of studied parameter depending on the gradation of each experimental factor (species, g...
Article
 Fertile cybrid plants of three subclones, B1A, B3A, B4A were regenerated from the single colony obtained after the fusion of mesophyll protoplasts of plastome chlorophyll-deficient mutant Lycopersicon peruvianum var 'dentatum' (line 3767) and γ-irradiated mesophyll protoplasts of L. esculentum (cv 'Quedlinburger Frühe Liebe'). Cytogenetic, isozyme...
Article
Photosynthetic characteristics of cytoplasmic hybrid Lycopersicon esculentum + L, peruvianum var. dentatum, which had been constructed by means of somatic hybridization, were investigated. This hybrid contained L. peruvianum var. dentatum Dun. plastome, reconstructed chondriome, and possessed phenotypic traits of nucleocytoplasmic incompatibility....
Article
The nature of a chlorophyll deficiency mutation in Lycopersicon peruvianum var dentatum was studied by cybridization. To obtain cytoplasmic hybrids, mesophyll protoplasts of the investigated mutant were fused with y-irradiated mesophyll protoplasts of L. esculentum. The resulting cybrid plants contained the nuclear genome of L. peruvianum var denta...
Article
Mesophyll protoplasts of species of series Juglandifolia (Solanum rickii, S. lycopersicoides, S. ochranthum and S. juglandifolium) were isolated and cultured in liquid nutrient media TM-2 or KM8P. The cell colonies formed were transferred onto agar-solidified media TM-3 or GM, and 10 to 15 days later onto TM-4, PRM, MS3ZG, KK or C regeneration medi...
Article
Fourteen shoots of a single cytoplasmic hybrid, obtained by fusion of protoplasts of cultivated tomato Lycopersicon esculentum (plastome chlorophyll-deficient mutant of the cultivar Friihe Liebe, 2n = 2x = 24) with γ-irradiated protoplasts of the wild tomato L. peruvianum var. dentatum (line 3767, 2n = 2x = 24), were grafted in the greenhouse for a...
Chapter
For Lycopersicon esculentum, symmetric intergeneric and interspecific somatic hybrids were regenerated following fusion with protoplasts of Solanum tuberosum (Melchers et al. 1978 ; Shepard et al. 1983), S. lycopersicoides (Handley et al. 1986; Tan 1987; Guri et al. 1991), S. rickii (O’Connell and Hanson 1986), S. nigrum (Guri et al. 1988), S. acau...
Article
Thirteen nuclear asymmetric hybrids were regenerated under selective conditions following fusion of chlorophyll-deficient protoplasts from cultivated tomato (Lycopersicon esculentum Mill.) and gamma-irradiated protoplasts from the wild species Lycopersicon peruvianum var. dentatum Dun. All hybrid plants were classified as being asymmetric based on...
Chapter
Lycium barbarum L. (2n = 24), is a woody bush spread all over Eurasia and North Africa. It is highly branched, 1–2.5 m high with single, reverse lanceolate smooth-edged leaves. The flowers are bell-shaped and violet-pink in color; the red, egg-shaped berries are edible. Wild L. barbarum forms a thicket, but it is often cultivated as an ornamental p...
Article
Leaf mesophyll protoplasts of chlorophyll-deficient Lycopersicon esculentum Mill. (plastome mutant Pl-alb 1) were fused in a fusion-inducing solution (PEG + Ca2+ + high pH) with mesophyll protoplasts of wild-type Lycopersicon peruvianum var. dentatum Dun. (lines 3767 and 3772) and interspecific nuclear and/or cytoplasmic hybrid plants were regenera...
Article
The possibility of plant regeneration from leaf tissue, callus and callus protoplasts of Lycium barbarum L. has been studied. Leaf segments were cultured on B5 medium (Gamborg et al. 1968) containing 1.5 mg/1 6-benzylaminopurine and 0.5 mg/1 α-naphthaleneacetic acid. Regeneration of shoots was initiated after 30 days of cultivation. Callus was obta...
Article
Protoplasts were isolated from leaves of the woody plant Lycium barbarum L. and cultured in liquid nutrient medium TM-2 at a density of 104–105 cells ml-1. After ten days of culture, regenerated colonies were transferred to the agar-solidified medium TM-3, and 5–7 days later to regeneration media PRM or TM-4. Formation of shoots was observed after...

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