Xupeng Cao

Xupeng Cao
Dalian Institute of Chemical Physics | DICP · State Key Laboratory of Catalysis

PhD

About

119
Publications
16,305
Reads
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2,091
Citations
Additional affiliations
January 2007 - present
Dalian Institute of Chemical Physics
Position
  • Professor
Education
September 2001 - July 2007
Dalian Institute of Chemical Physics
Field of study
  • biochemical engineering

Publications

Publications (119)
Article
Full-text available
The CO2 concentration at ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is crucial to improve photosynthetic efficiency for biomass yield. However, how to concentrate and transport atmospheric CO2 towards the Rubisco carboxylation is a big challenge. Herein, we report the self-assembly of metal-organic frameworks (MOFs) on the surface of...
Article
Photosynthesis, as the core of solar energy biotransformation, is driven by photosynthetic membrane protein complexes in plants and algae. Current methods for intracellular photosynthetic membrane protein complex analysis mostly require the separation of specific chloroplasts or the change of the intracellular environment, which causes the missing...
Preprint
Full-text available
The CO 2 concentration at ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is crucial to improve photosynthetic efficiency for biomass yield. However, how to concentrate and transport atmospheric CO 2 towards to the Rubisco carboxylation is a big challenge. Here in, we report the self-assembly of metal-organic frameworks (MOFs) on the surf...
Article
Full-text available
Chemoautotrophic bacterium Ralstonia eutropha H16 can fix CO2 to bioplastic and is potentially useful for CO2 neutralization. Targeting the solar fuel-based plastic biomanufactory, the polyhydroxybutyrate (PHB) production between heterotrophy and chemoautotrophy conditions was evaluated and the proteomic responses of the R. eutropha H16 cells to di...
Article
Full-text available
Light is the prerequisite for photosynthesis. However, excess light flux higher than the light-saturation point gives rise to photoinhibition or photodamage. To efficiently utilize the excess energy under the light saturation is a long-standing issue of photosynthesis. Herein, we found an electron drainage channel using artificial redox shuttle as...
Article
Full-text available
Accumulation of β-carotene in Dunaliella salina is highly dependent on light exposure intensity and duration, but quantitative analysis on photon numbers received per cell for triggering β-carotene accumulation is not available so far. In this study, experiment results showed that significant β-carotene accumulation occurred after at least 8 h illu...
Article
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Sponges are among the most primitive multicellular organisms and well-known as a major source of marine natural products. Cultivation of sponge cells has long been an attractive topic due to the prominent evolutionary and cytological significance of sponges and as a potential approach to supply sponge-derived compounds. Sponge cell culture is carri...
Article
Monogalactosyldiacylglycerols (MGDGs) have potential applications in food products, cosmetics and pharmaceuticals. MGDGs from microalgae with high amounts of polyunsaturated fatty acids (PUFAs) have potential functions, which arise the interest of the researchers. MGDGs were prepared by silica gel column chromatography with the appropriate mobile p...
Article
Salvia miltiorrhiza is one of the most commonly used medicinal materials in China. In recent years, the quality of S. miltiorrhiza has attracted much attention. Biotic and abiotic elicitors are widely used in cultivation to improve the quality of medicinal plants. We isolated an endophytic fungus, Mucor fragilis, from S. miltiorrhiza. We compared t...
Article
Full-text available
The stressed cultivations are widely used in microalgae R&D for the biofuel production with the repress on growth to a certain degree, which limits the overall productivity. The balance between the growth and energy storage compounds accumulation is a target needing the combination of both strain selection or construction and culture optimization....
Article
Full-text available
Microalgae is a promising organism as the feedstock of the next generation biofuels, as well as high value nature products, such as astaxanthin, normally under certain stress cultivation conditions. With the clear industrialization targets, there have been two waves of microalgae R&D from the last century and showed obvious energy-driven trends. Th...
Article
Full-text available
Monogalactosyldiacylglycerols (MGDGs) from microalgae containing a high proportion of polyunsaturated fatty acids (PUFAs) have enormous potential applications in various industries. In this study, the productivity and fatty acid composition of MGDGs from three microalgae in commercialized production, Chlorella sorokiniana GT, Nannochloropsis oceani...
Article
The enzymatic carbon fixation is a promising approach to deal with greenhouse gas emission and is usually accompanied with energy consumption during the reduction of CO2. As a very important route, the carboxylation can convert CO2 to organic carbon without extra requirement of reduction power and is hoped as a greener solution, especially for some...
Article
Full-text available
The increasing demand for triacylglycerol (TAG) enriching polyunsaturated fatty acids (PUFAs) has led to a surge of interest in microalgal TAG metabolism. Polar membrane lipids serve as the desaturation carrier for PUFA and the functional group PUFA can be incorporated into TAG. Monogalactoglycerolipid (MGDG) has been found to provide the de novo s...
Article
Full-text available
Background: Microalgal starch is regarded as a promising alternative to crop-based starch for biorefinery such as the production of biofuels and bio-based chemicals. The single or separate use of inorganic carbon source, e.g., CO2 and NaHCO3, caused aberrant pH, which restricts the biomass and starch production. The present study applied an in sit...
Article
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Triacylglycerols are considered one of the most promising feedstocks for biofuels. Phospholipid:diacylglycerol acyltransferase (PDAT), responsible for the last step of triacylglycerol synthesis in the acyl-CoA-independent pathway, has attracted much attention by catalyzing membrane lipid transformation. However, due to lack of biochemical and enzym...
Article
Full-text available
Background: Carbohydrates are major biomass source in fuel-targeted biorefinery. Arthrospira platensis is the largest commercialized microalgae with good environmental tolerance and high biomass production. However, the traditional target of A. platensis cultivation is the protein, which is the downstream product of carbohydrates. Aiming to provid...
Article
Full-text available
Abstract Isochrysis zhangjiangensis is widely used in the marine aquaculture as larval feed, especially for filter feeding cultures, as well as a good candidate for biofuels. However, the optimal cultivation temperature for I. zhangjiangensis is below 30 °C and this stain is seriously affected by high temperature, which causes the limited applicati...
Article
To understand the function of DGTS (diacylglyceryl-N,N,N-trimethyl-homoserine) as substitution of PC (phosphatidylcholine), Nannochloropsis oceanica has been chosen as the model system because it has DGTS and PC simultaneously. Phosphorus is a regulator for DGTS and PC. Generally, DGTS increases and correspondingly PC decreases under phosphorous st...
Article
Stress state of microalgal cells is caused under unfavorable conditions such as disordered light regime and depleted nitrogen. The stress state can impair photosynthetic efficiency, inhibit cell growth and result in the accumulation of triacylglycerol (TAG) from protective mechanisms. Continuous light or nitrogen starvation was applied on microalga...
Article
Full-text available
Glycerol-3-phosphate acyltransferase (GPAT) is considered as the rate-limiting enzyme of glycerolipid synthesis pathway and the core element in lysophosphatidic acid (LPA) synthesis. For understanding its catalytic mechanism, the structural biology study is expected, but is always hindered by obtaining crystals for X-ray diffraction analysis. In th...
Article
Haematococcus pluvialis accumulates high contents of astaxanthin and is considered as an important source of natural astaxanthin production. Fast and accurate quantification of astaxanthin is needed to evaluate the quality of microalgal sources for astaxanthin extraction and to monitor the H. pluvialis cultivation process. In the present study, a l...
Article
Comprehensively understanding enzymatic stereoselectivity will assist in the creation of new enzymes for producing optically pure compounds for chemical applications. The essential features for selecting enantiomers are controlled by particular residues or regions of the enzymes. We report a stereoselective mechanism in the D‐2‐haloacid dehalogenas...
Article
Microalgae are potential starch producers as alternatives to agricultural crops. This study disclosed the effects and mechanism of phosphorus availability exerted on storage starch production in a starch-producing microalga Tetraselmis subcordiformis in nitrogen starvation conditions. Excessive phosphorus supply facilitated starch production, which...
Article
In microalgae, triacylglycerol (TAG) biosynthesis occurs by parallel pathways involving both the chloroplast and endoplasmic reticulum. A better understanding of contribution of each pathway to TAG assembly facilitates enhanced TAG production via rational genetic engineering of microalgae. Here, using a UPLC-MS(/MS) coupled with TLC-GC-based lipido...
Article
Full-text available
Background: Triacylglycerol (TAG) from photosynthetic microalgae is a sustainable feedstock for biodiesel production. Physiological stress triggers microalgal TAG accumulation. However excessive physiological stress will impair the photosynthesis system seriously thus decreasing TAG productivity because of the low biomass production. Hence, it is...
Article
Full-text available
Background The synchronous triacylglycerol (TAG) production with the growth is a key step to lower the cost of the microalgae-based biofuel production. Phospholipid: diacylglycerol acyltransferase (PDAT) has been identified recently and catalyzes the phospholipid contributing acyl group to diacylglycerol to synthesize TAG, and is considered as the...
Article
Full-text available
Medium-chain fatty acids have attracted significant attention as sources of biofuels in recent years. Acyl-ACP thioesterase, which is considered as the key enzyme to determine the carbon chain length, catalyzes the termination of de novo fatty acid synthesis. Although recombinant medium-chain acyl-ACP thioesterase (TE) affects the fatty acid profil...
Article
Full-text available
D-2-haloacid dehalogenases (D-DEXs) catalyse the hydrolytic dehalogenation of D-2-haloacids, releasing halide ions and producing the corresponding 2-hydroxyacids. A structure-guided elucidation of the catalytic mechanism of this dehalogenation reaction has not been reported yet. Here, we report the catalytic mechanism of a D-DEX, HadD AJ1 from Pseu...
Article
Full-text available
With the fast development of microalgal biofuel researches, the proteomics studies of microalgae increased quickly. A filter-aided sample preparation (FASP) method is widely used proteomics sample preparation method since 2009. Here, a method of microalgae proteomics analysis based on modified filter-aided sample preparation (mFASP) was described t...
Article
Acyl-ACP thioesterase (TE) catalyzes the hydrolysis of thioester bonds during type II fatty acid synthesis and directly determines fatty acid chain length. Most TEs are responsible for recognition of 16:0 and 18:1 substrates, while specific TEs interrupt acyl-ACP elongation at C8-C14. However, the acyl selection mechanism of TE has not been thoroug...
Article
Full-text available
Triacylglycerols are produced in abundance through chloroplast and endoplasmic reticulum pathways in some microalgae exposed to stress, though the relative contribution of either pathway remains elusive. Characterization of these pathways requires isolation of the organelles. In this study, an efficient and reproducible approach, including homogeno...
Article
In a marine green starch-producing microalga Tetraselmis subcordiformis, the role of starch phosphorylase (SP) in the starch biosynthesis was disclosed by characterizing the enzyme properties and activity variations during the starch accumulation process. TsSP4, a SP isoform accounting for the major SP activity in T. subcordiformis, was unique to b...
Article
Nannochloropsis, an ocean microalgal genus, is a promising resource of biodiesel feedstock because of its capacity for triacylglycerol (TAG) accumulation. Polar glycerolipids have big contribution for TAG accumulation by providing acyl group or diacylglycerol (DAG). Due to the diversities and amount of polar glycerolipids in species, a suitable app...
Article
Microalgae are renewable feedstock for sustainable biofuel production, cell factory for valuable chemicals and promising in alleviation of greenhouse gas CO2. However, the carbon assimilation capacity is still the bottleneck for higher productivity. Molecular characterization of CO2 sequestration and assimilation in microalgae has advanced in the p...
Article
The production of poly-3-hydroxybutyrate (PHB) by photosynthetic cyanobacteria is a potentially sustainable production method for the biodegradable plastics industry. β-Ketoacyl-ACP reductase (FabG), from the photosynthetic cyanobacterium Synechocystis sp. PCC 6803 (SpFabG), is the first NADPH-dependent reductase in the fatty acid biosynthesis path...
Article
In the context of sustainable cultivation of microalgae, the present study focused on the use of nitrogen from the hot-water extracted biomass residue of Arthrospira platensis by hydrothermal carbonization (HTC) and the sequential cultivation of the same alga with the HTC aqueous phase (AP). Nearly 90% of the nitrogen recovered from HTC into AP was...
Article
Oxidative species and phytohormones are important factors in plants that can regulate stress responses. In this study, the content variations of four oxidative species, O2−, H2O2, ClO−, and NO, and the phytohormones, salicylic acid (SA), jasmonic acid (JA), and folic acid (FA), were investigated in the microalgae Isochrysis zhangjiangensis under ni...
Article
Sustainable microalgal cultivation at commercial scale requires nitrogen recycling. This study applied hydrothermal carbonization to recover N of hot-water extracted Arthrospira platensis biomass residue into aqueous phase (AP) under different operation conditions and evaluated the N utilization, biomass yield and quality of A. platensis cultures u...
Article
Full-text available
Isochrysis zhangjiangensis is a potential marine microalga for biodiesel production, which accumulates lipid under nitrogen limitation conditions, but the mechanism on molecular level is veiled. Quantitative real-time polymerase chain reaction (qPCR) provides the possibility to investigate the gene expression levels, and a valid reference for data...
Article
Full-text available
Background: Fatty acid composition is an important physiological parameter of microalgae, which is taken as the third generation alternative resource of biodiesel. To boost microalgal research and applications, a convenient, rapid, and acid-catalyzed transesterification procedure that satisfies the demand for the analysis of the fatty acid composi...
Article
2-Haloacid dehalogenases have been highly studied due to their potential applications in chemical industries and bioremediation. Although biochemical and structural characterizations of the enzyme have been detailed, no information was available regarding environmental effects on the structure-function relationship. Here, circular dichroism spectro...
Article
Full-text available
An aerobic, Gram-negative bacterium, strain 2-5T, was isolated from a crude oil-contaminated marine sponge collected near Dalian Bay, China, and subjected to a polyphasic taxonomic investigation. Cells of strain 2-5T were non-spore forming, non-motile, rods 0.2–0.3 µm wide and 1.1–1.2 µm long. Strain 2-5T grew well on nutrient agar, TSA, R2A agar a...
Article
PhaB (acetoacetyl-CoA reductase) catalyzes the reduction of acetoacetyl-CoA to (R)-3-hydroxybutyryl-CoA in polyhydroxybutyrate (PHB) synthesis and FabG (3-ketoacyl-acyl-carrier-protein reductase) catalyzes the β-ketoacyl-ACP to yield (R)-3-hydroxyacyl-ACP in fatty acid biosynthesis. Both of them have been classified into the same group EC 1.1.1.. P...
Article
Starch, synthesized by microalgae with utilization of light energy and carbon dioxide, is the sustainable feedstock for large scale production of fuel ethanol. In order to enhance accumulation of starch by genetic engineering, it is essential to understand catalytic process and regulation of the key enzymes of starch metabolism in microalgae. For t...
Article
Full-text available
The marine microalga Tetraselmis subcordiformis could photoproduce hydrogen under the regulation of carbonyl cyanide m-chlorophenylhydrazone (CCCP), and a hydrogen production process kinetic analysis was characterized by two peaks, suggesting that two distinct mechanisms might exist in this alga. Therefore, 2D nanoliquid chromatography-tandem mass...
Article
Full-text available
There was no direct correlation in substrate specificity between the metabolism of Pseudomonas stutzeri DEH138 and its corresponding dehalogenase. Dehalogenase substrates that could be dehalogenated might not be degraded by DEH138 or vice versa. Basing on this, different approaches to enhance L-2-haloacid dehalogenase (L-DEX) production in DEH138 v...