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Publications (4)
3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (DAHPS) is a key enzyme in the shikimate pathway for the biosynthesis of aromatic compounds. L-Phe and L-Tyr bind to the two main DAHPS isoforms and inhibit their enzyme activities, respectively. Synthetic biologists aim to relieve such inhibitions in order to improve the productivity of aromatic...
L‐Tyrosine derivatives are widely applied in the pharmaceutical, food, and chemical industries. Their production is mainly confined to chemical synthesis and plant extract. Microorganisms, as cell factories, exhibit promising advantages for valuable chemical production to fulfill the increase in the demand of global markets. Yeast has been used to...
Hydroxytyrosol (HT) is a valuable aromatic compound with numerous applications. Herein, we enabled the efficient and scalable de novo HT production in engineered Saccharomyces cerevisiae (S. cerevisiae) from glucose. Starting from a tyrosol-overproducing strain, six HpaB/HpaC combinations were investigated, and the best catalytic performance was ac...
While plastics bring convenience to industrial production and daily life, serious environmental problems are always accompanied. Polyurethane is an important polymeric material with versatile applications, but it also accounts for severe plastic contamination worldwide. Since it is difficult for polyurethanes to be completely degraded under natural...
Questions
Question (1)
Are there any method to insert a short sequence (less than 100bp) into another (maybe 500- 3000bp and in target) randomly?
