Xuetao Shi

South China University of Technology | SCUT · National Engineering Research Center for Human Tissue Restoration and Reconstruction Function

Ideal repair of intestinal injury requires a combination of leakage-free sealing and postoperative antiadhesion. However, neither conventional hand-sewn closures nor existing bioglues/patches can achieve such a combination. To this end, we develop a sandwiched patch composed of an inner adhesive and an outer antiadhesive layer that are topologicall...

The classical 3D-printed scaffolds have attracted enormous interests in bone regeneration due to the customized structural and mechanical adaptability to bone defects. However, the pristine scaffolds still suffer from the absence of dynamic and bioactive microenvironment that is analogous to natural extracellular matrix (ECM) to regulate cell behav...

The severe pain caused by oral ulcers seriously affects food intake and speech, bringing great inconvenience in daily life. Drug-loaded patches are mostly used to treat oral mucosal diseases such as oral ulcers and oral lichen planus, but their effects are limited because of the influences of saliva and muscle movement. To enhance the adhesion of d...

Introduction With the advances in skeletal muscle tissue engineering, new platforms have arisen with important applications in biology studies, disease modeling, and drug testing. Current developments highlight the quest for engineering skeletal muscle tissues with higher complexity that can better mimic in vivo situations. Among these new developm...

Both magnesium and lithium are able to stimulate osteogenic and angiogenic activities. In this study, lithium magnesium phosphate (Li0.5Mg2.75(PO4)2, Li1Mg2.5(PO4)2 and Li2Mg2(PO4)2) biomaterials were synthesized by a solid-state reaction method, and their bioceramic blocks and scaffolds were fabricated by compression molding and 3D printing, respe...

Natural gels are constrained by a limited number of building blocks, yet based on time and space organization, they perform diverse functions. In contrast, the properties of synthetic hydrogels are frequently tuned through substantial changes in their chemical make‐up, causing complex interplay between composition, structure, and properties. This w...

Polyacrylonitrile (PAN) nanofiberous membranes incorporated with different content of Curcumin (Cur), tannic acid and silver nanoparticles (AgNPs) were successfully fabricated by an electrospinning process. The analytical results from the characterizations including scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR...

Gallium-containing biomaterials are considered promising for reconstructing osteoporotic bone defects, owing to the potent effect of gallium on restraining osteoclast activities. Nevertheless, the gallium-containing biomaterials were demonstrated to disturb the osteoblast activities. In this study, tricalcium phosphate (TCP) bioceramics were modifi...

Human reproductive organs are of vital importance to the life of an individual and the reproduction of human populations. So far, traditional methods have a limited effect in recovering the function and fertility of reproductive organs and tissues. Thus, aim to replace and facilitate the regrowth of damaged or diseased tissue, various biomaterials...

Synthetic hydrogels are unique tissue mimics but rarely reproduce the strain-stiffening properties of native tissues. This mechanical mismatch impairs the performance of hydrogels in practical applications. Inspired by the crimped structure of collagenous tissues, a series of strain-stiffening hydrogels composed of curved parallel fibers are develo...

Fibronectin (Fn) is significant to the performance of biomaterials, and the chemistry of biomaterial surface play important roles in Fn adsorption and subsequent cell behavior. However, the “molecular scale” mechanism is still unclear. Herein, we combined experimental strategies with molecular simulations to solve this problem. We prepared self-ass...

Peptides are widely used for surface modification to develop improved implants, such as cell adhesion RGD peptide and antimicrobial peptide (AMP). However, it is a daunting challenge to identify an optimized condition with the two peptides showing their intended activities and the parameters for reaching such a condition. Herein, we develop a high-...

Dressing materials with wet adhesive and antibacterial features are in high demand for the healing of infected wounds. Here, we report the development of an antibacterial peptide (KRWWKWWRRC)-modified collagen nanosheet (KCN) by a two-step spin-coating process. The two-dimensional nanosheet demonstrates promising mechanical strength of ~31 MPa at a...

The decreasing number and reduced osteogenic differentiation capacity of mesenchymal stem cells (MSCs) and the excessive proliferation of osteoclasts in periodontal defects lead to difficulty in periodontal regeneration. Growth factors have powerful impacts on stem cell recruitment and differentiation, and alendronate (ALN) is a potent inhibitor of...

With the advent of an ageing society, bone-related diseases such as osteoporosis have become a major human health issue, and osteolytic lesions caused by cancer bone metastasis have always had a poor prognosis. Researchers have studied the pathogenesis of these orthopaedic diseases to obtain efficacious treatments. Microfluidic chip technology is a...

Bone is an important part of the human body structure and plays a vital role in human health. A microfluidic chip that can simulate the structure and function of bone will provide a platform for bone-related biomedical research. Hydroxyapatite (HA), a bioactive ceramic material, has a similar structure and composition to bone mineralization product...

Periodontitis is caused by host immune-inflammatory response to bacterial insult. A high proportion of pro-inflammatory macrophages to anti-inflammatory macrophages leads to the pathogenesis of periodontitis. As stem cell-derived exosomes can modulate macrophage phenotype, dental pulp stem cell-derived exosomes (DPSC-Exo) can effectively treat peri...

The construction of 3-dimensional (3D) scaffolds with spatially distributed cells throughout remains a challenge despite their high demand for use in tissue and organ repair/regeneration. To tackle this challenge, here we demonstrate a new strategy for building such scaffolds. First, osteoblast-seeded magnetic meshes (consisted of interconnected el...

Surface topography and chemical characteristics can regulate stem cell proliferation and differentiation, and decrease the bone-healing time. However, the synergetic function of the surface structure and chemical cues in bone-regeneration repair was rarely studied. Herein, a strontium ion (Sr2+)-substituted surface hydroxyapatite (HA) hexagon-like...

There is a consensus that the prevention of wound infection should be achieved in the following ways: (1) closing the wound to protect it from extra infection; (2) an antibacterial agent that could kill endogenous bacteria. However, existing bulk two-dimensional antibacterial materials show inefficient adhesion to wounds with complex morphology and...

Materials research usually relies on lengthy and largely trial-and-error methods, high-throughput technology has thereby emerged as an alternative method which is proven to be a simple, rapid, accurate and sensitive technique. Here, we presented a microfluidic platform with a set of 6 × 6 microarray chips for high-throughput synthesis and rapid scr...

Injury of corpus cavernosa results in erectile dysfunction, but its treatment has been very difficult. Here we construct heparin-coated 3D-printed hydrogel scaffolds seeded with hypoxia inducible factor-1α (HIF-1α)-mutated muscle-derived stem cells (MDSCs) to develop bioengineered vascularized corpora. HIF-1α-mutated MDSCs significantly secrete var...

Calcium phosphates (CaP) represent an impressive kind of biomedical material due to their excellent biocompatibility, bioactivity, and biodegradability. Their morphology and structure highly influence their properties and applications. Whilst great progress has been made in research on biomedical materials, there is still a need to develop a method...

Bioceramic-biopolymer composites have been used extensively as bone tissue engineering scaffolds due to their bioactive properties. However, composite scaffolds are insufficient in inducing osteogenic differentiation of stem cells. In this study, a strategy for the local delivery of bioactive factors that coating calcium alendronate (ALC) on the su...

Diabetics face multiple long-term health complications and diabetic foot ulcers affect approximately 15-20 % of the diabetic population. The high disability rate of these patients results in substantial mental burden and economic pressure to the individuals as well as the societies. Here, we report a therapeutic integrin-binding pro-survival peptid...

There is a great challenge in regenerating osteochondral defects because they involve lesions of both cartilage and subchondral bone, which have remarkable differences in their chemical compositions and biological lineages. Thus, considering the complicated requirements in osteochondral reconstruction, a biomimetic biphasic osteochondral scaffold (...

Healing soft-tissue wounds with an irregular, complicated topography in a bleeding environment demands the development of a dressing that is wet-adhesive, haemostatic, and antibacterial. To meet this unmet demand, we designed a flexible nanosheet (∼77 nm thick) made of two layers, one is the antibacterial and haemostatic gelatin modified with dopam...

In the preparation of bioactive bone graft materials, surface topography is essential for the ultimate stem cell response. However, the tunable fabrication of surface topography for 3D bioceramic scaffolds is still a technical problem because of the low processability and high brittleness of bioceramics. In this study, an evenly spaced hexagon-like...

Phosphorous-containing biopolymers have been applied to expedite the regeneration of damaged bone tissue by stimulating the function of phosphorous groups in natural bone. However, the underlying mechanism of phosphorous-containing biopolymers in promoting osteogenic differentiation is unclarified. Herein, we synthesized phosphorylated chitosan hyd...

Background: Localized delivery of antimicrobial agents such as antimicrobial peptides (AMPs) by a biomaterial should be on-demand. Namely, AMPs should be latent and biocompatible in the absence of bacterial infection, but released in an amount enough to kill bacteria immediately in response to bacterial infection. Methods: To achieve the unmet goal...

The remarkable difference in cell type and matrix composition between two connected parts of a joint (cartilage and subchondral bone) makes it challenging to simultaneously regenerate both parts for joint repair. Thus we chemically designed a biphasic hydrogel made of two well-bonded shape-tunable hydrogel phases, termed bone-regenerating hydrogel...

Natural polysaccharides and proteins have been widely explored for the preparation of hydrogel matrix due to their promising biocompatibility and biodegradability. However, it is challenge to achieve multiple functions of the hydrophilic matrix through convenient functionalization strategies. Herein we report the facile engineering of natural matri...

Limited dimensional stability and osteogenic induction property of poly (lactide-co-glycolide) (PLGA) electrospun membranes hampered their applications in bone tissue engineering. Thermal treatment of fixed PLGA membranes at 50 °C for 2 h and further immersion in 75% ethanol at free-standing state were adopted in order to obtain a high stability an...

Hospital‐acquired infection causes many deaths worldwide and calls for the urgent need for antibacterial biomaterials used in clinic that can selectively kill harmful bacteria. The present study rationally designs fusion peptides capable of undergoing 2D self‐assembly on the poly(methyl methacrylate) surface to form a smart surface, which can maint...

Novel 3D-printable hydrogels with host–guest non-covalent interactions and covalently crosslinked networks show robust mechanical strength, self-healing performance and excellent biocompatibility.

Skeletal muscle tissue engineering (SMTE) aims at repairing defective skeletal muscles. Until now, numerous developments are made in SMTE; however, it is still challenging to recapitulate the complexity of muscles with current methods of fabrication. Here, after a brief description of the anatomy of skeletal muscle and a short state‐of‐the‐art on d...

Melatonin (MT) has been served as promising osteogenic factor to promote osteogenesis. However, the direct injection of MT accompanies fast diffusion and rapid degradation, which could significant affect the repairing of bone defects. Sustainable release of MT from scaffolds would greatly enhance bone regeneration performance, but has never been st...

Inspired by the constitution of things in the natural world, three-dimensional (3D) nanofiber scaffold/cells complex was constructed via the combination of electrospinning technology and origami techniques. The nanofiber boxes prepared by origami provided a limited space for the layer-by-layer nanofiber films, and the human fetal osteoblasts (hFOBs...

Hydroxyapatite (HAP) is the foremost inorganic components of human hard tissues and has been widely applied as biomaterials for replacing or repairing. In this study, hydroxyapaptite (HAP) nanoparticles with controllable morphology were facilely synthesized by hydrothermal treatment of abandoned abalone shells without adding any organic template ag...

Phosphorylated biopolymers can induce mineralization, mimic the process of natural bone formation, and have the potential as scaffolds for bone tissue engineering. 2-Methacryloyloxyethyl phosphorylcholine (MPC), a low cytotoxicity phosphorus source, is mainly applied in vascularization and promoting blood compatibility and has been less researched...

By mimicking the role of phosphate groups in native tissues and encouraging tissue formation, phosphate-functionalized biopolymers have been widely employed to accelerate the formation of bone injury. In this study, phosphocreatine is screened as a phosphate source and applied in the preparation of phosphocreatine-modified chitosan (CS-P) and porou...

Front Cover: In article 1800130 by Ling Yan, Yan Li, and co‐workers, electrospun composite fibers in multi‐scale structures are formed, which contain microparticles with both hydroxyapatite (HAp) and CaCO3, and also casein, in a hierarchical structure. Random composite fibers significantly enhance proliferation and osteogenic differentiation of adi...

In a minimally invasive procedure, hydrogels can be injected into an affected area for drug loading and tissue repair. Here, gelatin methacryloyl (GelMA) was modified with alendronate (ALN), and three different alendronate-functionalized GelMA (GelMA-ALN) hydrogels were prepared. The modification greatly improved the swelling ratio, protein adsorpt...

s β‐tricalcium phosphate/phosphate‐based glass (β‐TCP/PG) composite spheres were prepared by an extrusion‐spheronization method featuring high production and fine control of sphere size. Subsequently, fully interconnected β‐TCP composite ceramic sphere‐based (TCCS) scaffolds were fabricated by sintering the randomly packed β‐TCP/PG composite sphere...

It is still a challenge to achieve both excellent mechanical strength and biocompatibility in hydrogels. Here we exploited two interactions to form a novel biocompatible, slicing‐resistant and self‐healing hydrogel. One is the molecular host‐guest recognition between a host (isocyanatoethyl acrylate‐modified β‐cyclodextrin, β‐CD‐AOI2) and a guest (...

Phosphorylated materials are attractive candidates for bone regeneration because they may facilitate the construction of a phosphorylated bone extracellular matrix (ECM) to build a beneficial environment for bone formation. Here, we designed and synthesized a new phosphorylated material, collagen type I phosphorylated with alendronate sodium (Col-A...

Bioactive scaffolds with interconnected porous structures are essential for guiding cell growth and new bone formation. In this work, we successfully fabricated three-dimensional (3D) porous \(\upbeta \)-tricalcium phosphate (\(\upbeta \)-TCP)/calcium silicate (CS) composite scaffolds with different ratios by 3D printing technique and further inves...

It is still a challenge to achieve both excellent mechanical strength and biocompatibility in hydrogels. Here we exploited two interactions to form a novel biocompatible, slicing‐resistant and self‐healing hydrogel. One is the molecular host‐guest recognition between a host (isocyanatoethyl acrylate‐modified β‐cyclodextrin, β‐CD‐AOI2) and a guest (...

Infections associated with biomedical implants and devices pose a serious clinical challenge to hospitals worldwide. The antimicrobial peptides (AMPs) become the prospect to inhibit this infection due to their broad-spectrum antimicrobial activity and low cytotoxicity. However, it is still the bottleneck to apply AMPs on the biomaterial surface, as...

Uniform Casein/CaCO3 microspheres were fabricated with well tuned properties and the formation mechanism based on the templating effect of casein micelles was proposed. In bone tissue engineering, vaterite microspheres were promising because of the biocompatibility and being easily fabricated. Here Casein/CaCO3 microspheres were precipitated by mix...

Graphene and its derivatives, along with adipose-derived stem cells (ADSCs), have demonstrated great potential to be used in neuron tissue engineering. ADSCs from rats (rADSCs) were first isolated and the surface markers were evaluated using flow cytometry. The rADSCs were fibroblast-like, positively expressed CD90 and CD105, lacked surface markers...

Hydroxyapatite (HAP) has been widely used for repairing or substituting human hard tissues. In this paper, two typical ionic surfactants, cation hexadecyltrimethylammonium bromide (CTAB) and anion sodium dodecyl sulfate (SDS), were used for rapid conversion of HAP from abalone shell. From field emission scanning electron microscopy (FESEM), the pre...

A well-designed scaffold with accurately controlled structure, pore size as well as interconnection plays a key role in tissue engineering. In this study, porous Li-containing biphasic calcium phosphate scaffolds (Li-BCP) with expected structure were fabricated successfully by 3D plotting technique. The results of scanning electron microscopy (SEM)...

In this study, we developed a simple approach for the controllable growth of whitlockite (WH) on a β-tricalcium phosphate surface and investigated its cell viability via CCK-8, its live-dead staining and its alkaline phosphatase activity. Herein, WH with controllable morphologies was prepared by regulating the hydrothermal reaction conditions. The...

Poly(lactide-co-glycolide) (PLGA) electrospun composite nanofibers were fabricated to mimic bone extra cellular matrix for applications in bone tissue engineering. Poly(acrylic acid) modified Zn-doped calcium phosphate (PAA-CaP/Zn) nanoparticles were first synthesized. PAA was selected because of the strong bonding ability with calcium and Zn was i...

Biomimetic mineralized hybrid scaffolds are widely used as natural bone substitute materials in tissue engineering by mimicking vital characters of extracellular matrix (ECM). However, the fabrication of hybrid scaffolds with suitable mechanical properties and good biocompatibility remains a challenge. To solve the problems mentioned above, biomime...

Nanosized celluloses are attractive building blocks to generate hierarchically advanced materials and have been gradually explored in emulsion applications. Here we report a high internal phase emulsion (HIPE) prepared by using supramolecular cellulose nanocrystals (CNCs) as Pickering stabilisers via one-step emulsification, and interconnected macr...

Polymer sphere-based scaffolds, which are prepared by bonding the adjacent spheres via sintering the randomly packed spheres, feature uniform pore structure, full three-dimensional (3D) interconnection, and considerable mechanical strength. However, bioceramic sphere-based scaffolds fabricated by this method have never been reported. Due to high me...

Antimicrobial peptides (AMPs) are a broad prospect for clinical application against bacterial infections of biomaterials. However, a bottleneck exists as there is a lack of simple technology to prepare AMPs on biomaterials with sufficient activity, as the activity of AMP is dependent on the correct orientation on the biomaterial. In the present stu...

Osteopontin (OPN) is a key mediator of cell interactions with biomaterials. However, few studies have been dedicated to studying cell adhesion on OPN-adsorbed substrates with controlled charge and wettability. Here, amino-carboxyl (NH2/COOH) and hydroxyl-methyl (OH/CH3) mixed self-assembled monolayers (SAMs) of varying charges and wettability, resp...

Material properties and cellular behaviours seemed to be coupled, implying the existence of reciprocities between cells and materials. Understanding cellular behaviours and material structure is indispensable for materials synthesis and design but has mostly focused on shape, neglecting the status of the sub-structure. Hence we utilized a similar r...

Ceramic-metal composite materials, namely cermets, are provided with characteristics of both ceramic and metal. Herein, for the first time bio-cermets based on β-tricalcium phosphate (β-TCP) bioceramic with biodegradable iron being reinforcement phase, were fabricated using the powder metallurgic method. The phase composition, microstructure, mecha...

Spherical hydroxyapatite(HAP) crystals with hierarchical structure and controllable size has been successfully synthesized by hydrothermal method. The polyvinylpyrrolidone(PVP) as a template united with Ca²⁺ chelating agent is used to regulate the nucleation and crystal growth. The synthesized powders were characterized by X-ray diffraction (XRD),...

Control over the reversible assembly of micro- and nano-scopic building blocks is highly important but very challenging in the fabrication of many hierarchical composite materials. Here we report the reversible assembly of emulsion micro-droplets stabilized by graphene oxide/polyvinyl alcohol (GO/PVA) hybrid via simply controlling the hydrogen bond...

Technical limitations of traditional electrospinning make it hard to produce three-dimensional (3D) scaffolds with hierarchical pore structures. Here, porous polycaprolactone (PCL) nanofibre meshes with different nano-hydroxyapatite (nHA) concentrations were prepared by electrospinning with stainless steel mesh as the collector, and 3D porous nanof...