Xiyin Wang

Mayo Clinic - Rochester · Department of Biochemistry and Molecular Biology

To investigate the feasibility of genetic screening for deafness causative genes in the process of newborn hearing screening in China. Total 865 newborn babies between November 2009 and March 2010 were enrolled for the simultaneous hearing and deafness causative gene screening in Tongji Hospital, Wuhan, China. Hearing screening followed a two-stage...

The highly repetitive Alu retroelements are regarded as methylation centres in the genome. Methylation in the gene promoters could be spreading from them. Promoter methylation of MLH1 is frequently detected in cancers, but the underlying mechanism is unclear. The aim of this study is to understand whether the methylation in the Alu elements is asso...

To investigate audiometric characteristics of hearing loss in a large Chinese ethnic Tujia family and determine its hereditary type. Total 76 live individuals were investigated in the notable 84 members of this family. The detailed audiometric evaluations were undertaken for the proband and his 47 family members. The degrees of sensorineural hearin...

Introduction Endometriosis, a benign inflammatory disease whereby endometrial-like tissue grows outside the uterus, is a risk factor for endometriosis-associated ovarian cancers. In particular, ovarian endometriomas, cystic lesions of deeply invasive endometriosis, are considered the precursor lesion for ovarian clear-cell carcinoma (OCCC). Method...

The human endometrium exhibits complex signaling cascades mediated by spatial–temporal cellular interactions. Understanding the normal endometrial microenvironment is the first step towards understanding diseases of endometrial dysfunction.

Endometriosis is a chronic gynecologic disease that negatively affects the quality of life of many women. Unfortunately, endometriosis does not have a cure. The current medical treatments involve hormonal manipulation with unwanted side effects and high recurrence rates after stopping the medication. Sadly, a definitive diagnosis for endometriosis...

Recent sequencing studies showed that loss-of-function mutations in ARID1A (AT-rich interactive domain 1a) were enriched in gynecologic malignancies, and oncogenic KRAS mutations are a common finding in endometrial cancers. However, neither of these genetic insults alone was sufficient to develop gynecologic cancer. To determine the role of the com...

We created and characterized the first genetically engineered mouse model of poorly-differentiated endometrial adenocarcinoma (dcKO, PgrCre; Ptenflox/flox; Dicer1flox/flox). As an additional novel model system, using CRISPR-Cas9, we created and characterized an isogenic cell line with the deletion of ( −/− Ishikawa cells). Transcriptomic profiling...

Recent sequencing studies showed that loss-of-function mutations in ARID1A (AT-rich interactive domain 1a) were enriched in gynecologic malignancies. However, multiple mouse models with deletion of Arid1a did not exhibit gynecologic malignancy. Oncogenic KRAS mutations are a common finding in endometrial cancers. However, expression of oncogenic Kr...

Endometriosis occurs when endometrial-like tissue grows outside the uterine cavity, leading to pelvic pain, infertility, and increased risk of ovarian cancer. The present study describes the optimization and characterization of cellular spheroids as building blocks for Kenzan scaffold-free method biofabrication and proof-of-concept models of endome...

Endometriosis occurs when endometrial-like tissue grows outside the uterine cavity, leading to pelvic pain, infertility, and increased risk of ovarian cancer. The present study describes the optimization and characterization of cellular spheroids as building blocks for Kenzan scaffold-free method biofabrication and proof-of-concept models of endome...

Endometrial cancer remains the most common gynecological malignancy in the United States. While the loss of the tumor suppressor, PTEN (phosphatase and tensin homolog), is well studied in endometrial cancer, recent studies suggest that DICER1, the endoribonuclease responsible for miRNA genesis, also plays a significant role in endometrial adenocarc...

Endometrial cancer remains the most common gynecological malignancy in the United States. While the loss of the tumor suppressor, PTEN (phosphatase and tensin homolog), is well studied in endometrial cancer, recent studies suggest that DICER1 , the endoribonuclease responsible for miRNA genesis, also plays a significant role in endometrial adenocar...

Endometrial cancer is the most common gynecologic malignancy in the United States and the sixth most common cancer in women worldwide. Fortunately, most women who develop endometrial cancer have low-grade early-stage endometrioid carcinomas, and simple hysterectomy is curative. Unfortunately, 15% of women with endometrial cancer will develop high-r...

Background and Hypothesis: Endometrial cancer is the most common gynecological malignancy in the US and will claim the lives of over 12,000 women in 2019. Women with high-risk histologic tumors, such as high-grade endometrial adenocarcinoma, represent over 50% of these deaths. Novel treatments are needed to treat these aggressive tumors. Decreased...

This commentary highlights the article by Fang et al that describes the role of enhancer of zeste homolog 2 in endometrial development.

Women with endometriosis, a benign growth of endometrial tissue outside the uterine cavity, are at increased risk of specific histotypes of epithelial ovarian cancer, such as ovarian endometrioid adenocarcinoma (OEA). Women with OEA who have endometriosis at time of surgical staging demonstrate improved clinical prognosis compared to women with OEA...

Women with endometriosis are at increased risk of developing ovarian cancer, specifically ovarian endometrioid, low-grade serous, and clear-cell adenocarcinoma. An important clinical caveat to the association of endometriosis with ovarian cancer is the improved prognosis for women with endometriosis at time of ovarian cancer staging. Whether endome...

Women with endometriosis can suffer from decreased fecundity or complete infertility via abnormal oocyte function or impaired placental-uterine interactions required for normal pregnancy establishment and maintenance. Although AT-rich interactive domain 1A (SWI-like) (ARID1A) is a putative tumor suppressor in human endometrial cancers and endometri...

MicroRNA (miR) eru stuttar RNA-sameindir sem taka þátt í að stjórna genatjáningu. Þær geta þáttaparast við mRNA og hindrað próteinmyndun. Í nokkrum æxlisgerðum á sér stað minnkuð tjáning á miR451 og því má ætla að um sé að ræða orsakasamband við framvindu æxlisvaxtar. Í þessari rannsókn var unnið að því að skilgreina starfsemi miR451 í æxlisbælifer...

IL6 was originally identified as a B-cell differentiation factor. It is a multifunctional cytokine that regulates the immune response, haemopoiesis, the acute phase response and inflammation. IL6 is produced by various types of cells and influences various cell types, and has multiple biological activities through its unique receptor system. IL6 ex...

The miR-451 was found to be frequently down-regulated in tumors, indicating that miR-451 could play an important role in carcinogenesis. This study uncovered the mechanism by which the miR-451 functions as a tumor suppressor. The target genes of miR-451 were determined using target gene prediction softwares. Then the miR-451 mimics were introduced...

Acute myeloid leukemia (AML) is the most common malignant myeloid disorder of progenitor cells in myeloid hematopoiesis and exemplifies a genetically heterogeneous disease. The patients with AML also show a heterogeneous response to therapy. Although all-trans retinoic acid (ATRA) has been successfully introduced to treat acute promyelocytic leukem...

FZD has no effects on cell-cycle in AML cells. Cell-cycle assessment in the tested acute myeloid leukemic cell lines using propidium iodine (PI) detected by flow cytometry at 24 hours. (TIF)

p21 is dispensable in FZD-mediated inhibition of NB4 cell proliferation. (A) The p21 mRNA expression was measured by RT-PCR in AML cell lines after 72 hours treatment with the predetermined IC50 value for FZD treatment or control (DMSO). (B) Knockdown of p21 expression in NB4 cells was assayed using real time PCR. (C) MTS assay was used to measure...

Aim: Our previous study showed that methylation of the mutL homolog 1 (MLH1) promoter may spread upstream from the Alu elements in intron 1. In this study, we investigated if the Alu methylation could also spread downstream. Two colorectal cancer cell lines (RKO, SW48), and four colorectal and three gastric carcinomas [all Microsatellite Instabilit...

It is known that RNA editing occurs in human cells, which can change the information transmission from DNA to RNA and proteins. Most previous studies have focused on editing of the mRNAs. Here we reported that several kinds of RNAs, including miRNA, rRNA, mRNA, miscRNA and unknown RNA, exhibited base editing in a human fetal liver. Several editing...

Oligonucleotide sequences of the primers for methylation analysis. (DOC)