Xinjian Shan

Shandong University | SDU

Background The development of specific antibodies against human leukocyte antigen (HLA) and/or human platelet antigen (HPA) could induce platelet transfusion refractoriness especially in patients receiving multiple platelet transfusions. A retrospective analysis was conducted to evaluate the prevalence of platelet-specific antibodies and the effica...

We describe the identification of a novel allele HLA-A*03:01:09 in a Chinese individual.

The HLA-B*55:34 allele differs from the closest matching allele B*55:02:01 by one nucleotide substitution in exon 2.

The novel HLA-B*39:46 differs from HLA-B*39:01:01:01 by one base substitution at position 27(C>G) of exon 4.

The sequence of a novel allele, HLA-B*40:124:02, differs from HLA-B*40:42 by three-nucleotide exchanges in exon 3.

The HLA-B*39:42 allele differs from the closest matching allele B*39:01:01:01 by two nucleotide substitutions in exon 3.

The sequence of HLA-B*27:44 differs in exon 2 from the sequence of B*27:08 by one amino acid change at codon 74.

The sequence of HLA‐B*40:01:06 differs in exon 3 from the sequence of B*40:01:01 by one nucleotide 435 (codon121) G to A.

The novel HLA-A*24:128 shows one nucleotide change from HLA-A*24:02:01:01 in exon 2 at position 155 from A to G.

The novel allele differs from HLA-B*07:02:01 by a single nucleotide substitution at position 538 in exon 3.

The HLA-B*13:23 allele differs from the B*13:01:01 by one nucleotide substitution in exon 2 at nt 97.

The HLA-B*54:16 allele differs from the B*54:01 by one nucleotide substitution in exon 3 at NT412.

The B*13:18 allele differs from the most closely matching allele B*13:02:01 by one nucleotide substitution in exon 3, at position 539.

The A*24:02:17 allele differs from the most close matching allele A*24:02:01:01 by one nucleotide substitution in exon 2, at position 264.

A novel human leukocyte antigen (HLA)-DRB1 allele, DRB1*08:36, has been identified during routine sequence-specific oligonucleotide (SSO) typing and sequence-based typing (SBT) in a sample from a registered donor of Chinese Marrow Donor Program. The DRB1*08:36 allele differs from the closest related DRB1*08:03:02 allele by one nucleotide substituti...

We describe the identification of a new DRB1*14 allele, DRB1*1461, found in a Chinese individual. The novel allele has been identified in routine polymerase chain reaction-sequence-specific oligonucleotide and sequence-based typing. The nucleotide sequence of DRB1*1461 is identical to DRB1*1404 except for a single substitution in codon 16 (TAT-->CA...

In this report, we describe the identification of a novel allele HLA-A*0128, which was found in a registered donor from China Marrow Donor Program. The A*0128 allele differs from the A*0113 by one nucleotide substitutions in exon 2 at position 282 (G-->C). The mutation results in a codon change: at codon 70 where a glutamine acid is substituted by...

Eleven novel human leukocyte antigen (HLA) alleles were identified during routine sequence-specific oligonucleotide probe (SSOP) typing (LABType; One Lambda Inc., Los Angeles, CA) of volunteers for a hematopoietic stem cell registry in Beijing, China. The new alleles were detected when one or more probes gave an unexpected reactivity pattern.

A novel human leukocyte antigen (HLA) class I allele, HLA-B*3713, has been identified in a Chinese individual. The HLA-B*3713 allele differs from the closest matching allele B*370101 by one nucleotide substitutions in exon 3 at nt 527(T-->A), resulting in an amino acid change from Val (GTG) to Glu (GAG) at codon 152.

The tyrosine kinase ZAP-70 has been implicated as a critical intermediary between T-cell antigen receptor (TCR) stimulation and Erk activation on the basis of the ability of dominant negative ZAP-70 to inhibit TCR-stimulated Erk activation, and the reported inability of anti-CD3 antibodies to activate Erk in ZAP-70-negative Jurkat cells. However, E...

A rationally mode-locked 10 GHz erbium fiber laser stabilized by a simple technique for extended time operation is described. The laser generates stable ~ 5 ps pulses that can be used in communications experiments. Back-to-back bit error rate (BER) measurements confirmed error-free operation for 6-8 hours, giving an error floor below 10 -14 . A rec...

The transcription factor NF-kappaB is a critical regulator of T cell function that becomes strongly activated in response to coengagement of TCR and CD28. Although events immediately proximal to NF-kappaB activation are well understood, uncertainty remains over which upstream signaling pathways engaged by TCR and CD28 lead to NF-kappaB activation....

To study the incidence and mechanism of the tumor suppressor gene p16 and RB inactivation in lung cancer, and to investigate their relations to biological characteristics, clinical pathological diagnosis and gene classification. Immunohistochemistry, double in situ hybridization, PCR, PCR-SSCP, and sequencing were used to study the changes in the p...

Mode-locked fibre lasers are a promising pulse source for use in high-speed data communication systems. Optical pulses at high repetition rates can be achieved by mode-locking the laser cavity at a rational harmonic of the fundamental mode-locking frequency. Using this technique, optical pulse streams at very high rates can be obtained by using lon...

Pleckstrin homology (PH) domain binding to D3-phosphorylated phosphatidylinositides (PI) provides a reversible means of recruiting proteins to the plasma membrane, with the resultant change in subcellular localization playing a key role in the activation of multiple intracellular signaling pathways. Previously we found that the T-cell-specific PH d...

To study the incidences and mechanisms of tumor suppressor gene p16 and Rb inactivation in lung cancers, and to investigate their relation to biological characters, clinical pathological diagnosis and gene classification diagnosis of lung cancers. Immunohistochemistry, double in situ hybridization, PCR, PCR-SSCP and sequencing were applied to study...

To clone CDKN2/p16(INK4a) gene, prepare its probe, and to study the change of CDKN2/p16(INK4a) gene in lung cancers. Total RNA of normal lung tissue was extracted, CDKN2/p16(INK4a) gene cDNA synthesized, and CDKN2/p16(INK4a) gene recombinant vector, constructed. Southern blot was used to study CDKN2/p16(INK4a) gene in 46 cases of lung cancers, 3 ca...

To study the relationship between methylation state of CDKN2/p16 gene and lung cancers. Genomic DNA was digested with methylation-sensitive enzymes and Southern blotting was used to analyses the methylation state of CDKN2/p16 gene in 89 cases of lung cancer. Twenty-one out of 89 lung cancers(23.6%) shown methylation of CDKN2/p16 gene, and 17 of the...

The authors report straight-line single-channel 40 Gbit/s RZ data transmission over 240 km of standard fibre with periodic dispersion compensation. To the authors' knowledge, this is the longest transmission distance reported in a straight-line transmission experiment, at 40 Gbit/s without any precoding. The back-to-back receiver sensitivity of the...

To study the relationship between CDKN2/p16 gene homozygous deletion and lung cancer progression. Improved multiplex PCR technique was applied to detect deletion of CDKN2/p16 gene exon1 and exon2 in 89 cases of lung cancers. Gene deletion rate was increased by the improved PCR technique. Exon 1 and exon 2 deletion rates were 19.1% (17/89) and 22.5%...

The Tec family tyrosine kinase, Itk has been implicated in T cell antigen receptor (TCR) signaling, yet little is known about Itk regulation. Here, we investigate the role of the tyrosine kinase ZAP-70 in regulating Itk. Whereas Itk was activated in Jurkat T cells in response to CD3 cross-linking, Itk activation was defective in the ZAP-70-deficien...

In the design of distributed erbium-doped fibers (DEDFs) for ultra-high bit-rate (>40 Gbit/s) soliton transmission, polarization mode dispersion (PMD) is an important design parameter. We have measured the PMD values of DEDFs from two different sources and found them to be an order of magnitude higher than those suitable for ≥40 Gbit/s soliton tran...

This paper shows how the initial linear birefringence determines the necessary spinning parameters to produce spun fiber with optimum differential group delay (DGD) and polarization mode dispersion (PMD) properties. DGD measurements are reported on two pairs of fibers, each pair having been fabricated from a particular fiber preform. The fiber pair...

We present, what we believe are, the first polarisation mode dispersion (PMD) measurements reported on distributed erbium doped fibres (DEDF), together with theoretical modelling of PMD and discuss their implications for high bit rate soliton transmission over DEDF amplifier links

We show how a conventional OTDR, modified in a simple way can be used to detect, from its power spectrum, those sections of a fibre link where some fibre twist exists, and to obtain the intrinsic polarization differential group delay (DGD) of the untwisted fibre. We derive the theory which underlies the above technique and present experimental resu...

In this paper, we make the first report of what we believe is the first fully polarization-sensitive, real-time optical time domain reflectometer (OTDR), which can measure the full polarization-scattering matrices along the fiber, leading to the evaluation of both the linear- and twist-induced circular birefringence distributions along the fiber. T...

A very simple method is proposed and demonstrated to stabilise an actively modelocked erbium fibre laser with a nonpolarisation maintaining cavity. This method is based on the detection of differential growth rates of noise at different frequencies, which is then used to control cavity length. The whole control circuit is composed of low frequency...

Not Available

Soliton communications technology has become a promising candidate for the next generation of high speed long-haul optical transmission systems, mainly due to the rapid development of discrete erbium-doped fibre amplifiers (EDFA). However, the requirement that the amplifier spacing must be much smaller than the soliton period may make the amplifier...

Multiwavelength channel NRZ to RZ format conversion using a single semiconductor laser amplifier is proposed and experimentally demonstrated. Bit-error-rate measurement of four 10 Gbit/s converted RZ signal channels has confirmed that no noticeable error floor existed

The authors demonstrate, for the first time, a three node optically time division multiplexed network with a maximum capacity of 40 Gbit/s. Electro-optical switches were used for both `drop and insert' and demultiplexing, and electronic clock recovery provided synchronisation throughout the network

Soliton communication technology has attracted widespread interest in recent years. Both theoretical and experimental research has demonstrated that soliton systems have the capability of transmitting signals at multi-Gbit/s capacities over transoceanic distances. A key component in such a system is the picosecond pulse source, whose quality has a...

2.5 Gbit.s^-1 solitons have been transmitted a distance of 17600 km in a recirculating loop without transmission control. To achieve this a new stabilisation technique for modelocked erbium fibre lasers has been implemented. Cavity length changes are tracked with an electronic phaselock loop and the phase of the RF drive-adjusted accordin...

Four independently modulated 10 Gbit/s channels were transmitted over 205 km with a penalty of less than 1.5 dB. The pulse source comprised a stabilised 10 GHz erbium fibre ring laser, multiplexed to 40 Gbit/s in a single polarisation by four independent lithium niobate modulators. Following transmission the requirement to kick start the demodulato...

A novel method to suppress noise in a high harmonic mode-locked erbium fibre laser is proposed and realised for the first time, dithering the cavity length at a kilohertz rate. This method makes only one supermode in the laser dominant and suppresses the others. A fibre laser so stabilised has enabled 2.5 Gbit/s soliton transmission over 205 km fib...

This paper pays particular attention to the 40 Gbit/s test bed developed to assess the performance of the polymer demultiplexer. In addition to the basic performance of the testbed, the requirement of satisfactory performance for all transmitted channels, rather than just a single channel, and the start-up difficulties involved in demodulating this...

Stable picosecond optical pulse sources working at multigiga hertz repetition rates are highly desirable for applications in soliton communication systems. Mode-locked erbium fibre lasers can produce highly transform limited pulses, but unless synchronisation to specific data rates can be demonstrated, they are of little use in communications exper...