Xiaoqing Lin

GuangDong University of Technology

The biggest challenge of utilizing lignocellulosic feedstocks for efficient ethanol fermentation is to improve strains and processes capable of mixed sugar fermentation in the presence of released inhibitors. A previously reported Saccharomyces cerevisiae strain WXY70 was further engineered to overexpress up to eight xylose metabolic genes or a tra...

In this study, three kinds of amide functional group modified hyper-cross-linked adsorption resin were synthesized by alternating radical copolymerization in simple one-step and applied for 5-hydroxymethylfurfural (5-HMF) adsorption. The successful synthesis and properties of adsorbents were evaluated with Fourier transform infrared spectroscopy, s...

In these studies, a low-cost and energy efficiency production of cellulosic ethanol from sugarcane bagasse (SCB) using one-pot without solid-liquid separation, water washing, and detoxification was performed. Firstly, SCB was pretreated using liquid hot water as the only reagent at 210 oC for a short time (0 min), and the solid liquid ratio (SLR) w...

5-hydroxymethylfurfural (5-HMF) is a promising high value-added platform chemical, which can be produced from glucose, fructose, or lignocellulosic biomass via catalysis technology. However, the effective separation of 5-HMF from aqueous solution and actual biomass hydrolysate is still challenging because 5-HMF can be further rehydrated into levuli...

This study investigated cellulosic ethanol production from sugar cane bagasse (SCB) pretreated by triethylbenzyl ammonium chloride/lactic acid (TEBAC/LA) deep eutectic solvent (DES). The results showed that the pretreatment of SCB with TEBAC/LA DES at 120 oC for 4 h with 1:15 (solid to liquid ratio) resulted in the best cellulose digestibility (88....

Biobutanol is one of the most promising renewable biofuels, which can be produced from lignocellulosic biomass by Clostridium. However, due to the dense structure of lignin, biomass is difficult to be hydrolyzed into fermentable sugars. This study has developed a short time (90 min) and low energy consumption (120 ◦C) pretreatment process using dee...

Worldwide, there are various feedstocks such as straws, corn stover, sugarcane bagasse, sweet sorghum bagasse (SSB), grasses, leaves, whey permeate, household organic waste, and food waste (FW) that can be converted to valuable biofuels such as butanol. For the present studies, an economic analysis was performed to compare butanol production from t...

Lignocellulosic hydrolysate contains complex nonsugar compounds and undegraded sugars in the process of preparing platform compound levulinic acid (LA) and furfural by one-step dilute-acid hydrolysis. For efficiently and comprehensively utilizing the hydrolysate, a series of polar modified resins were synthesized for adsorption and separation of th...

Efficient and feasible pretreatment of lignocellulosic biomass waste is an important prerequisite step to promote subsequent enzymatic hydrolysis and enhance the economics of biofuels production. This study focuses on the pretreatment of wheat straw (WS) with triethylbenzyl ammonium chloride/lactic acid (TEBAC/LA)-based deep eutectic solvents to en...

The purification of lignocellulosic hydrolysate has great practical significance in the field of bioenergy and biorefinery. In this study, the effects of textural properties of styrene-divinylbenzene (St-DVB) adsorption resin prepared by oil/water suspension polymerization method on its adsorption capacities of sugar, fermentation inhibitors, and p...

BACKGROUND A series of monoacrylate modified adsorption resins from reactive mixtures with different amounts and types of monoacrylate have been synthesized by an oil/water (O/W) suspension polymerization technique and utilized to remove fermentation inhibitors and pigments from rice straw hydrolysate for the first time. The modified resin was char...

Four oleaginous yeasts (Trichosporon coremiiforme, Trichosporon dermatis, Cryptococcus albidus, and Trichosporon cutaneum) were used for treatment of the wastewater after butanol fermentation. The wastewater containing high COD value (33.70 g L⁻¹) and total sugar concentration (13.07 g L⁻¹) could satisfy the requirement of cellular growth. Among th...

Lumping kinetics models were built for the biological treatment of ABE (acetone-butanol-ethanol) fermentation wastewater by oleaginous yeast Trichosporon cutaneum with different fermentation temperatures. Compared with high temperature (33°C, 306 K) and low temperature (23°C, 296 K), medium temperature (28°C, 301 K) was beneficial for the cell grow...

Background Microbial oil is one important bio-product for its important function in energy, chemical, and food industry. Finding suitable substrates is one key issue for its industrial application. Both hydrophilic and hydrophobic substrates can be utilized by oleaginous microorganisms with two different bio-pathways (“de novo” lipid fermentation a...

A self-synthesized HQ-8 resin was prepared using a O/W suspension polymerization technique and employed as a potential adsorbent for the removal of acid soluble lignin (ASL) from rice straw hydrolysate (RSH). The structure and morphology of the HQ-8 resin before and after the adsorption of ASL were characterized by Fourier transform infrared spectr...

To fulfill the comprehensive utilization of cellulose and hemicellulose components in bagasse for bacterial cellulose (BC) production, both bagasse acid and enzymatic hydrolysates were used for BC production by Gluconacetobacter xylinus. Although the BC accumulation rate was slower during the early period of fermentation in the bagasse acid hydroly...

Lithium chloride (LiCl)/N,N-dimethylacetamide (DMAc) solvent system was used to dissolve native and pretreated wheat straw materials in order to promote the enzymatic hydrolysis process. The dissolution ratio of wheat straw in LiCl/DMAc solvent system increased when dilute sulfuric acid or ethanol-sulfuric acid mixture pretreatment was conducted be...

Objective To investigate the inhibiting effect of formic acid on acetone/butanol/ethanol (ABE) fermentation and explain the mechanism of the alleviation in the inhibiting effect under CaCO3 supplementation condition. ResultsFrom the medium containing 50 g sugars l−1 and 0.5 g formic acid l−1, only 0.75 g ABE l−1 was produced when pH was adjusted by...

The purification of lignocellulose hydrolysate is crucial for the efficient conversion of lignocellulose. In this study, weak polar poly(styrene-divinyl benzene-methyl acrylate) (PSDM) and composite absorbent org-attapulgite/poly(styrene-divinyl benzene-methyl acrylate) (PSDM-T) were prepared in situ by suspension polymerization for the detoxificat...

ABE (acetone- butanol- ethanol) fermentation wastewater was treated by mono-culture or binary co-culture of three oleaginous yeasts belonging to genus Trichosporon (Trichosporon cutaneum, Trichosporon dermatis, and Trichosporon coremiiforme). Considering biomass and lipid content, co-culture showed little beneficial effect possibly due to the earli...

Extraction of high-value products from agricultural wastes is an important component for sustainable bio-economy development. In this study, wax extraction from sugarcane bagasse was carried out and the beneficial effect of dewaxing pretreatment on the enzymatic hydrolysis was investigated. 1.2% (w/w) of crude sugarcane wax was obtained from the su...

In this study, semi-pilot scale microbial oil production by Trichosporon cutaneum using medium containing corncob acid hydrolysate was carried out in a 50-L fermentor. Scale up showed no negative influence on lipid fermentation that no obvious lag phase was observed. Both glucose and xylose could be utilized simultaneously by T. cutaneum, but the u...

In present study, the beneficial effect of corncob acid hydrolysate environment on lipid fermentation of Trichosporon dermatis was elucidated by method of cell biology (mainly using flow cytometry and microscope) for the first time. Propidium iodide (PI) and rhodamine 123 (Rh123) staining showed that corncob acid hydrolysate environment was favorab...

Elephant grass (Pennisetum purpureum) dilute acid hydrolysate contains 34.6 g/L total sugars. The potential of lipid production by oleaginous yeast Trichosporon cutaneum grown on elephant grass acid hydrolysate was investigated for the first time. During the fermentation process on the elephant grass acid hydrolysate, glucose, xylose and arabinose...

To overcome the xylose utilization defect in ethanol fermentation by wide-type Saccharomyces cerevisiae and alleviate the carbon catabolite repression (CCR) in acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum, a novel mixed fermentation of S. cerevisiae and C. acetobutylicum was developed. When S. cerevisiae was inoculated 2...

In the present study, a novel micro-mesoporous hyper cross linked polymeric adsorbent, SY-01, was tested to remove several inhibitory compounds from butanol fermentation wastewater (BFW) for bio-butanol production for the first time. Characterization of the SY-01 resin was determined by scanning electron microscope (SEM), nitrogen adsorption-desorp...

Unlabelled: To reduce the cost of bacterial cellulose (BC) production, the possibility of using acetone-butanol-ethanol (ABE) fermentation wastewater with high COD value (18 050 mg l(-1) ) for BC production by Gluconacetobacter xylinus was evaluated. After 7 days of fermentation, the highest BC yield (1·34 g l(-1) ) was obtained. The carbon source...

In this study, corncob acid hydrolysate was used as a substrate for bacterial cellulose (BC) production by Gluconacetobacter xylinus. After 2 weeks' static fermentation, a BC yield of 4 g/L could be obtained. Both effects of medium composition and fermentation condition on the BC production were evaluated. Most extra substrates (carbon and nitrogen...

In this work, D-II07 resin was used to remove formic acid from aqueous solution. A series of operational conditions including solution pH, adsorbent dose, temperature and initial formic acid concentration on formic acid uptake was evaluated. D-II07 resin showed a good uptake at neutral pH. Removal of formic acid was rapid and equilibrium was reache...

Although litchi has both nutrient and edible value, the extremely short preservation time limited its further market promotion. To explore processed litchi products with longer preservation time, litchi extract was selected as an alternative feedstock for production of bacterial cellulose (BC). After two weeks' static fermentation, 2.53 g/L of the...

In this work, acetic acid was found as one promising substrate to improve xylose utilization by Gluconacetobacter xylinus CH001. Also, with the help of adding acetic acid into medium, the bacterial cellulose (BC) production by G. xylinus was increased significantly. In the medium containing 3 g l−1 acetic acid, the optimal xylose concentration for...

Disclosed is a method for separating butanol. The method uses hydrophobic macroporous polymer adsorbent to separate butanol in a mixed solution, and the process comprises the following steps: 1) using macroporous polymer adsorbent to adsorb butanol in a mixed solution; 2) desorbing butanol from macroporous polymer adsorbent. The method is simple; t...

In this work, corncob acid hydrolysate and its simulated medium whose sugar composition was the same as the corncob acid hydrolysate were used as fermentation substrate for lipid production by oleaginous yeast Trichosporon dermatis. On the corncob acid hydrolysate, after seven days' fermentation, the biomass, lipid content, lipid yield and lipid co...

Clostridium acetobutylicum can propagate on fibrous matrices and form biofilms that have improved butanol tolerance and a high fermentation rate and can be repeatedly used. Previously, a novel macroporous resin, KA-I, was synthesized in our laboratory and was demonstrated to be a good adsorbent with high selectivity and capacity for butanol recover...

For the first time, corncob acid hydrolysate was used for microbial oil production by the oleaginous yeast Lipomyces starkeyi. After hydrolysis by dilute sulfuric acid, corncob could turn into an acid hydrolysate with a sugar concentration of about 42.3 g/L. Detoxified by overliming and absorption with activated carbon, the corncob hydrolysate coul...

Removal of biobutanol from acetone-butanolethanol (ABE) fermentation broth can be achieved by fixed-bed sorption by means of KA-I resin, and the relevant breakthrough curves would provide much valuable information to help design a continuous fixed-bed sorption process in field application. In the present study, the effects of several important desi...

An improved, simple gas chromatography-flame ionization detection (GC-FID) method was developed for measuring the products of acetone-butanol-ethanol (ABE) fermentation and the combined fermentation/separation processes. The analysis time per sample was reduced to less than 10 min compared to those of a conventional GC-FID (more than 20 min). The b...

Computational modeling is a useful approach for the characterization, development, and validation of desorption chromatography. Batch and dynamic desorption experimental results are helpful for the selection of an appropriate desorbent and the design of a desorption processes. In this study, we used an aqueous HCl solution ranging from 0.030 mol/L...

A novel polar macroporous adsorption resin (AX-1) has been applied for separation of d-lactic acid (DLA) from aqueous solutions in a fixed-bed chromatographic column. The adsorption equilibrium and kinetics were investigated experimentally. A column model was subsequently established and validated by comparison of the simulation results to the expe...

The traditional distillation method for recovery of butanol from fermentation broth is an energy-intensive process. Separation of butanol based on adsorption methodology has advantages in terms of biocompatibility and stability, as well as economy, and therefore gains much attention. However, the application of the commercial adsorbents in the inte...

Computational and theoretical modeling has become an important tool for the characterization, development and validation of packed beds. Relevant breakthrough curves would provide much valuable information on designing a fixed bed adsorption process in field applications. In this study, the hydrodynamic properties involved in the Navier–Stokes flow...

BACKGROUND: Owing to the rapid depletion of petroleum fuel, the production of bio-butanol has attracted much attention. However, low butanol productivity severely limits its potential industrial application. It is important to establish an approach for recovering low-concentration butanol from fermentation broth. Experiments were conducted using ba...

Abstract BACKGROUND: Owing to the rapid depletion of petroleum fuel, the production of bio-butanol has attracted much attention. However, low butanol productivity severely limits its potential industrial application. It is important to establish an approach for recovering low-concentration butanol from fermentation broth. Experiments were conducted...

The adsorption behavior of uridine 5′-monophosphate (UMP) on a gel-type anion-exchange resin SD3 at different temperatures was investigated by the batch method. The dissociation equilibrium of UMP in aqueous solution has been studied. Effects of solution pH, contact time, and initial concentrations of UMP on the adsorption have been discussed. Adso...

In a batch equilibrium system, the maximum adsorption capacity of cAMP onto an anion-exchange resin reached 0.1718, 0.1956, 0.2764 and 0.3437 g g−1 at 283, 293, 303 and 313 K, respectively. The adsorption data obtained were well described by the Langmuir isotherm. In the batch kinetic system, Fick diffusion, pseudo first-order and pseudo second-ord...