Xiaoqing Chen

Central South University | CSU · Department of Chemistry Engineering

Background Thyroid eye disease (TED) is a vision-threatening autoimmune disorder. Orbital tissue fibrosis leading to intractable complications remains a troublesome issue in TED management. Exploration of novel therapeutic targets and agents to ameliorate tissue fibrosis is crucial for TED. Recent work suggests that Ca ²⁺ signaling participates in...

German chamomile is one of the most effective herbal elements used in anti‐allergic products and as an antioxidant. Herein, the antioxidant activity of different extract fractions of German chamomile was initially evaluated using an off‐line 2,2‐diphenyl‐1‐picrylhydrazyl spectrophotometric assay. The ethyl acetate extract demonstrated the highest e...

By serving dipyridylic acid (DPA) and 2,5-dihydroxyterephthalic acid (DHTA) as the biligands, a novel lanthanide (Eu³⁺) metal–organic framework (MOF) namely Eu-DHTA/DPA was prepared for specific Hg²⁺ fluorescence determination. The dual-ligand approach can endows the resulting luminescent MOF with dual emission of ratiometric fluorescence and unifo...

Photoinduced generation of phosphoranyl radicals offers a versatile strategy to access a variety of synthetically valuable radicals, which has opened exciting new opportunities for radical manipulations and synthetic chemistry. While β-scission mode of phosphoranyl radicals has been intensively studied and broadly utilized via deoxygenation or desu...

Rapid component separation and accurate identification of bisphenols compounds (BPs) in real water sample remain an attractive challenge due to the trace amounts and structural similarities of BPs, and complexity of real samples. Here, we designed and synthesized chemically modified cellulose p-toluenesulfonate (CTSA) to encapsulate octadecylamine-...

The cost-effective, convenient, visible, and equipment-free determination of biomarkers is always the priority development concern of disease diagnosis. The paper-based signal output strategy permits output visual signals without instruments and is regarded as a promising approach with simple operation and low cost. Herein, by varying the addition...

Due to low optical activity and structural simplicity, synchronous chiral discrimination and identification of aliphatic amino acids (AAs) are still challenging yet demanding. Herein, we developed a novel surface-enhanced Raman spectroscopy (SERS)-based chiral discrimination-sensing platform for aliphatic AAs, in which l- and d-enantiomers are able...

Herein, by employing a novel synthesized ligand H2L, a flower-like luminescent metal-organic framework IRMOF-3-L was constructed for developing a triple-mode sensor for glyphosate (Glyp) detection. The ligand H2L was designed to contain three functional parts, which endowed the resulted IRMOF-3-L with peroxidase-like activity and unique fluorescenc...

In situ rapid detection of contaminants in environmental water is crucial for protecting the ecological environment and human health; however, it is always hindered by the complexity of sample matrices, trace content, and unknown species. Herein, we demonstrate a deep learning-based multicapturer surface-enhanced Raman scattering (SERS) platform on...

In this work, a single-atom nanozyme- Rh-N/C was constructed with excellent tetra-enzyme mimic activities including laccase- (Km = 0.10 mM), oxidase (OXD; Km = 0.19 mM)-, superoxide dismutase (SOD)- and catalase (CAT)-like activities. Meanwhile, the laccase- and oxidase-mimic activities of Rh-N/C display excellent stability and reusability even und...

Real-time in-situ visualization and sensitive detection of thimbleful tumor-related microRNAs (miRNAs) in living cells is still unmet. Especially, poor amplification efficiency and resistance against complex intracellular environment hinder amplifiers’ widespread applications in vivo. Herein, we report enzyme-free self-propelled quantum dots-based...

A dual-channel probe was developed, based on a novel composite metal organic frameworks (ZnMOF-74@Al-MOF) for glyphosate determination through ratio fluorescence and colorimetric methods. The prepared probe can not only recognize and combine glyphosate by introducing copper ion into the MOF, but also possess peroxidase-like catalytic activity. The...

Identification of small-molecule binding sites in proteins is of great significance in analysis of protein function and drug design. Modified sites can be recognized via proteolytic cleavage followed by liquid chromatography-mass spectrometry (LC-MS); however, this has always been impeded by the complexity of peptide mixtures and the elaborate synt...

Purpose: Experimental autoimmune uveitis (EAU) is a representative animal model of human uveitis. In this study, we investigated whether apolipoprotein A1 (APOA1) can alleviate EAU and explored its underlying mechanism. Methods: Mice were immunized with interphotoreceptor retinoid-binding protein 1-20 and treated with APOA1 or vehicle. The retin...

Au(I)-thiolate complexes are a new class of aggregation-induced emission (AIE) material. Here we demonstrate a new aggregation strategy of water-soluble Au(I)-thiolate complexes induced by cationic polymers at optimized pH values. The generated AIE shows longer wavelengths than the emission induced by other methods.

Designing chiral adsorption separation material with high enantioselectivity and stability is a promising route to realize the enantioselective recognition and separation of racemic molecules. Here, for the first time, camphor sulfonic acid derivatives (CSA) were synthesized as a chiral resolving agent, and it was modified on Mg/Al layered double h...

Background Melatonin, an indoleamine produced by the pineal gland, plays a pivotal role in maintaining circadian rhythm homeostasis. Recently, the strong antioxidant and anti-inflammatory properties of melatonin have attracted attention of researchers. We evaluated the therapeutic efficacy of melatonin in experimental autoimmune uveitis (EAU), whic...

The diagnosis of vitreoretinal lymphoma (VRL), a rare subtype of primary central nervous system lymphoma (PCNSL), is challenging. We aimed to investigate the mutational landscape of VRL by sequencing circulating tumor DNA (ctDNA) from aqueous humor (AH) and/or vitreous fluid (VF), as well as the application of ctDNA sequencing to diagnosis and trea...

Background Melatonin, an indoleamine produced by the pineal gland, plays a pivotal role in maintaining circadian rhythm homeostasis. Recently, the strong antioxidant and anti-inflammatory properties of melatonin have attracted attention of researchers. We evaluated the therapeutic efficacy of melatonin in experimental autoimmune uveitis (EAU), whic...

Obesity is closely related to exacerbated graft rejection and worse graft survival. High leptin levels, caused by excessive adipose cells in obese individuals, may exert a pivotal role in the pathogenesis of allograft rejection. However, the role and underlying mechanism of leptin in allograft rejection remains unclear. This study explored the role...

Rapid and reliable detection of disease-related biomarkers in whole blood remains a great challenge due to the complex composition of blood. Here, we report a novel point-of-care test (POCT) platform for direct detection of biomarkers in blood sample, in which horseradish peroxidase (HRP) and 3,3',5,5'-tetramethylbenzidine (TMB) embedded poly(ethyl...

Autoimmune uveitis (AU) is a sight-threatening ocular inflammatory disorder, characterized by massive retinal vascular leakage and inflamed lesions with infiltration of the uveitogenic T cells in the retina and disorders of the T cell-related immune response in the system. Stimulation of TCRs can trigger calcium release and influx via Ca2+ channels...

Objective Vitreoretinal lymphoma (VRL) is a rare but aggressive masquerade syndrome, with challenging diagnosis. Since the tumor-related genetic mutation analysis based on cell-free DNA (cfDNA) are underutilized in ocular oncology, we aimed to test the VRL diagnostic value of cfDNA genetic mutation analysis using intraocular fluid (IOF) samples and...

Herein we report a framework nucleic acid programmed strategy to develop nanocarriers to precisely and independently package multiple homo- and heterogeneous cargos in vitro and in vivo, thereby enabling multiplexed...

Uveitis is one of the most common blindness-causing ocular disorders. Due to its complicated pathogenesis, the treatment of uveitis has been widely recognized as a challenge for ophthalmologists. Recently, the anti-inflammatory properties of the antibiotic Azithromycin (AZM) have been reported. However, the therapeutic effects of Azithromycin in ex...

Effective discrimination and quantitation of amino acid (AA) enantiomers is enormously pivotal in diverse fields ranging from pharmaceutics to biological systems. We herein proposed a one-step strategy to construct a novel cascade bioreactor for chiral sensing of multiple AA enantiomers by coupling L-amino acid oxidase (LAAO) and horseradish peroxi...

Corneal lymphangiogenesis induced by macrophages played a critical role in corneal allograft rejection (CGR). However, there are few Food and Drug Administration (FDA)-approved drugs that target lymphangiogenesis. The aim of our study is to evaluate the effects of dimethyl fumarate (DMF) on corneal allograft survival in rats. Penetrating corneal tr...

We herein report a facile and scalable strategy for the fabrication of a metal-organic framework (MOF) based composite by in situ growing ZIF-8 on gold nanoparticle (AuNP) loaded magnetic carbon nanotubes (mCNTs). AuNPs were firstly loaded on PEI (polyethylenimine) modified mCNTs by electrostatic forces, and then AuNPs/mCNTs were encapsulated into...

Background Anti-tumor necrosis factor-alpha was regarded as an option in treatment of non-infectious uveitis. However, few studies in the far easter region have concentrated on this therapy and current studies have not emphasized the elimination of retinal vasculitis. To compare the effectiveness of adalimumab (ADA) plus conventional therapy (CT) v...

Rapid component separation and reliable surface-enhanced Raman scattering (SERS) detection of organochlorine pesticide (OCP) residues in real water samples remain major challenges because of sample complexity, trace content, and low molecular affinity for a metal surface. Here, we report a novel strategy of simultaneous in situ extraction and fabri...

IL-23/IL-23R and PGE2/EP2+EP4 have been recognized as crucial signals that promote Th17 differentiation in many autoimmune diseases, including thyroid-associated ophthalmopathy (TAO). However, the interactive role of IL-23R in IL-23/Th17 signaling and PGE2/Th17 signaling has not been clarified in TAO. Furthermore, the role of IL-38, a novel anti-in...

In this work, a novel NiFe layered double hydroxide–derived sulfide (NiFeSx)-modified g-C3N4 nanosheet photocatalyst (NiFeSx/g-C3N4) was synthesized, and its morphology, structure and visible light absorption capacity were simultaneously characterized by XRD, SEM, TEM, FT-IR, XPS, UV-Vis DRS, PL techniques and EIS Nyquist plots. Furthermore, it was...

A series of functional IRMOF-3 frameworks with solid-state luminescent and tuneable light emission (from 490 to 608 nm) have been synthesized by per-functionaling AIE-active Schiff-bases with zinc. These precursor AIE-active ligands endowed the functional frameworks boosted fluorescent emission efficiencies (from 0.16% to 1.03%). The IRMOF-3-h reve...

By embedding high quantum yield blue-emitting carbon dots (CDs) into zeolitic imidazolate framework-8 (ZIF-8), the luminescent metal-organic frameworks (LMOFs) - [email protected] composites were fabricated facilely and served as the fluorescent probe for selective and sensitive detection of dopamine (DA). In which the sustainable pores within ZIF-...

Fabrication of carbon quantum dots (CQDs) as versatile fluorescence probes for simultaneous detection of cobalt ion (Co²⁺) in various samples is still unmet. Herein, novel boron, nitrogen-doped carbon quantum dots (B,N-CQDs) were facile synthesized via one-step hydrothermal method, in which 3-carboxyphenylboronic acid and ethylenediamine were selec...

Autoimmune uveitis (AU), being one of the sight-threatening ocular inflammatory disorders, has been widely regarded by ophthalmologists and immunologists as a great challenge. Apremilast, a phosphodiesterase-4 inhibitor (PDE4i), which was approved by the U.S. Food and Drug Administration (FDA) for the treatment of active psoriatic arthritis in 2014...

Low-cost, high-throughput, broadly useful photoresponsive enantiomeric excess (ee) sensing of amino acids remains challenging to date. Herein, based on the selective oxidation reaction of amino acid oxidase (AAO) to amino acid enantiomers (D/L-AA) and the oxidation reaction of substrate (H2O2) with aromatic boronic ester, we put forward a photoresp...

Sulfate radicals (SO4⁻)-based advanced oxidation processes have obtained far-reaching attention due to their high catalytic activity and selectively for intricate water environmental pollutants. The couple of cheap and readily available iron-based materials and peroxymonosulfate (PMS) or persulfate (PS) is an effective measure to generate incalcula...

Colorimetric analytical strategies exhibit great promise in developing on-site detection methods for antibiotics, while substantial recent research efforts remain problematic due to dissatisfactory sensitivity. Taking this into account, we develop a novel colorimetric sensor for in-field detection of antibiotics by using aptamer (Apt)-capped and ho...

Purpose: To characterize the phenotypic abnormalities of peripheral B cells in patients with Graves’ orbitopathy (GO) and explore the role of chemokine CXC ligand 13 and its receptor type 5 (CXCL13/CXCR5) in relation to B-cell homeostasis using specific neutralizing antibodies. Methods: Adults with active GO (n = 22), inactive GO (n = 28), and heal...

By binding aggregation-induced emission luminogens (AIEgens) with nanoscale ZIF-8, a series of novel Luminescent metal-organic frameworks (LMOFs) with good photostability and excellent dispersibility have been facilely fabricated. The developed AIE-active MOFs not only can inherit the intriguing AIE properties of AIEgens, that including excited-sta...

Purpose We conducted a systematic review and meta-analysis to determine the effectiveness and safety of anti-tumor necrosis factor-alpha (TNF-α) agents in the treatment of Behcets’ disease (BD)-associated uveitis. Method Three electronic databases, Embase, MEDLINE, and the Cochrane Library, were searched for eligible papers focusing on the anti-TN...

Facing the increasing organic wastewater pollutants in the world, it is curious to discover novel materials for efficient wastewater separation and organic pollutants degradation. Hence, in order to solve the above problems, zinc tungstate nanoparticle/nickel-aluminum-layered double hydroxides/polyvinylidene fluoride composite photocatalytic membra...

A hyphenated strategy by off‐line coupling 1, 1’‐diphenyl‐2‐picrylhydrazyl ‐HPLC, high‐speed counter‐current chromatography and preparative HPLC was established to screen and separate antioxidants from ethyl acetate fraction of the roots of Polygonum multiflorum. Under the target‐guidance of 1, 1’‐diphenyl‐2‐picrylhydrazyl‐HPLC experiment, twelve c...

The leaves of Rubus suavissimus (also called “Chinese sweet tea”) is used not only as a beverage tea and food additive but also as a folk herbal medicine for the treatment of diabetes mellitus. To systematically investigate its material foundation of efficacy for treating diabetes, herein, a hyphenated strategy by off-line coupling high-speed count...

Autoimmune uveitis (AU), a sight-threatening intraocular disorder, is still a challenge for ophthalmologists in clinic. Teriflunomide has been approved for multiple sclerosis (MS) in 2012 for its immunoregulatory function. However, the effect and mechanisms of teriflunomide in uveitis are still unknown. In this investigation, we used a murine model...

Therapeutic manipulation of regulatory T cells (Tregs) has been regarded as a promising approach for the treatment of immune disorders. However, a better understanding of the immunomodulatory mechanisms of Tregs and new safe and effective methods to improve the therapeutic effects of Tregs are highly desired. In this study, we have identified the k...

In this work, by adopting polyethyleneimine functionalized multi-walled carbon nanotubes as the absorbent, a solid phase extraction based method for fast and selective screening of carboxylic acid compounds was developed. The structure and morphology of the polyethyleneimine functionalized multi-walled carbon nanotubes were confirmed via scanning e...

The highly sensitive determination of ofloxacin (OFL) in human serum and urine was achieved on a novel tryptophan‐graphene oxide‐carbon nanotube (Trp‐GO‐CNT) composite modified glassy carbon electrode (Trp‐GO‐CNT/GCE). The Trp‐GO‐CNT composite was fabricated, and its morphologies and surface functional groups were characterized by field emission sc...

Facile and cost-effective detection of multiple targets is essential for a variety of applications ranging from life sciences to environmental monitoring. Here, we report a versatile ratiometric fluorescence platform for multiple detection of various targets based on the conjugation of single-stranded DNA (ssDNA) with protonated graphitic carbon ni...

Introduction Potentilla kleiniana Wight et Arn is widely used as a herbal medicine to treat type 2 diabetes. However, detailed information about its active compounds is lacking. Objective To develop an efficient method for the rapid screening and separation of α‐glucosidase inhibitors from Potentilla kleiniana Wight et Arn. Methodology Potential...

A new strategy by converging ultrafiltration high‐performance liquid chromatography‐ultraviolet‐mass spectrometry and pH‐zone‐refining counter‐current chromatography was developed for the rapid screening and separation of potential acetylcholinesterase inhibitors from the crude alkaloidals extract of Zanthoxylum nitidum. An optimized two‐phase solv...

The study on inhibitory effects of resveratrol glucosides (REs) on advanced glycation endproducts (AGEs) formation is still unmet. Herein, for the first time, the antiglycation activities of five REs in the fetal bovine serum proteins (FBS)/fructose system were evaluated, and its structure-activity relationship and antiglycation mechanism were furt...

Surface enhanced Raman scattering (SERS) substrates that can be attached to rough, irregular surfaces and directly collect samples is especially useful for the detection of surface organic residues. Herein, novel AuNPs-pseudo-paper films (APPFs) with uniform structure, flexible properties and wicking capabilities were first fabricated and used as S...

Nitrogen-doped carbon dots (N-CDs) were prepared by adjusting the ratio of carbon sources (citric acid) and nitrogen sources (ethanolamine). This N-CDs fluorescent probe had been successfully applied to rapidly, sensitively and selectively determine the concentration of mercury ion (Hg²⁺) with a linear range of 0.1–5.0 μM and a limit of detection o...

A facile one-step hydrothermal method was developed to synthesize nitrogen-doped carbon quantum dots (N-CQDs) by utilizing hexamethylenetetramine as the carbon and nitrogen source. The quantum yield (QY) of 21.7% was under the excitation wavelength of 420 nm with maximum emission at 508 nm. This N-CQD fluorescent probe has been successfully applied...

Background Nowadays, the combination of microRNA (miR) is attracting increased attention in clinical cancer trials. However, the clinical use of miR is highly limited because of certain properties such as instability, low-specificity distribution, and metabolic toxicity. Methods In order to improve the anti-tumor efficacy and reduce the side effec...

Purpose: To explore the roles of hemin in preventing corneal allograft rejection (CGR) and the underlying mechanisms. Methods: Hemin (30 mg/kg) was intraperitoneally injected into rats with a corneal allograft on alternate days, from the day of transplantation until euthanasia. The clinical signs of the corneal allografts were evaluated and reco...

Contamination of foods by aflatoxin B1 (AFB1) is a common serious problem. To improve the efficiency of AFB1 detection, this study aims to develop a sensitive aptasensor for detection of AFB1 in food samples based on the catalytic effect of aptamer/G-quadruplex DNAzyme probe. The resulting reassembly of this probe in the presence of hemin and K+ ca...

An ultrasensitive and highly reproducible novel surface-enhanced Raman scattering (SERS)-enabled capillary platform was developed for on-site detection of methotrexate (MTA) in serum. By employing a facile mirror reaction and a self-assembly procedure, glass capillaries were compactly coated with core-shell-satellite microspheres (CSSM) via a batch...

Programmierte Gerüstnukleinsäuren (FNAs) sind Teil einer Strategie für die Entwicklung valenzkontrollierter Signalverstärker mit hoher Modularität für die hoch empfindliche Biosensorik. X. Zuo et al. demonstrieren in ihrer Zuschrift (DOI: 10.1002/ange.201802701), wie die FNA‐programmierten Signalverstärker Nukleinsäuren, Proteine und anorganische N...

Ultrasensitive biosensing is made possible by a framework nucleic acids (FNA)‐programmed strategy, designed to develop valence‐controlled signal amplifiers with high modularity. In their Communication (DOI: 10.1002/anie.201802701), X. Zuo et al. demonstrate that the FNA‐programmed signal amplifiers could recruit nucleic acids, proteins, and inorgan...

In this work, phosphorus, nitrogen co-doped carbon quantum dots (P,N-CQDs) as highly selective fluorescent probe for cobalt ion (Co²⁺) detection were designed and synthesized via a one-step hydrothermal protocol by employing pyridoxal 5-phosphate and ethanediamine. This probe enables selective detection of Co²⁺ with a linear range of 0–60 μM and a...

The leaves of Malus hupehensis have a strong antioxidant activity and are commonly consumed as a healthy tea. However, detailed information about its antioxidants is incomplete. Herein, we developed an effective strategy based on combining off‐line two‐dimensional high‐performance liquid chromatography with ultraviolet and tandem mass spectrometry...

Wastewater discharged in human daily life, and oil spill accidents occurred frequently. The novel interface materials used for controllable oil/water separation have gained more and more attention. In this work, a facile, environmentally friendly approach is presented to fabricate a magnetic textile fabric with pH-controllable wettability between s...

Autoimmune uveitis is a sight-threatening ocular inflammatory disorder. Immunological inflammation is regarded as the key to pathogenesis in autoimmune uveitis. Baicalin, the major bioactive component of Scutellaria baicalensis, possesses immunomodulatory properties. However, the role of baicalin in uveitis and its underlying mechanisms remain uncl...