Xiao ping Pu

Xiao ping Pu
Peking University | PKU · School of Pharmaceutical Sciences

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111
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Introduction

Publications

Publications (111)
Article
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Degeneration and death of dopaminergic neurons in the substantia nigra of the midbrain are the main pathological changes in Parkinson’s disease (PD); however, the mechanism underlying the selective vulnerability of specific neuronal populations in PD remains unclear. Here, we used single-cell RNA sequencing to identify seven cell clusters, includin...
Article
Neuropathic pain (NP) is a prevalent clinical problem for which satisfactory treatment options are unavailable. Tetrandrine (TET), a bisbenzylisoquinoline alkaloid extracted from Stephania tetrandra S. Moore, possesses anti-inflammatory and immune-modulatory properties. Chemokine-like factor 1 (CKLF1) is known to play a crucial role in both periphe...
Article
Full-text available
Traditional Chinese medicine (TCM) fundamentally different from Western medicine, has been widely investigated using various approaches and made great contributions to the prevention and treatment of human diseases. In recent years, research about quality control, safety evaluations, and determination of mechanisms of TCMs focused on the single-cel...
Article
Parkinson's disease (PD) is a progressive neurodegenerative disorder that occurs after Alzheimer's disease. Rotenone is a neurotoxin commonly used in creating PD models. Safflower (Carthamus tinctorius L.) contains some flavonoids that are effective against neurodegenerative diseases, and it has long been used in the treatment of cerebrovascular di...
Article
Full-text available
Neuroinflammation, oxidative stress, and mitochondrial dysfunction are all important pathogenic mechanisms underlying motor dysfunction and dopaminergic neuronal damage observed in patients with Parkinson’s disease (PD). However, despite extensive efforts, targeting inflammation and oxidative stress using various approaches has not led to meaningfu...
Article
Full-text available
Background: Notoginseng leaf triterpenes (PNGL) is believed to have neuroprotective effects via the inhibition of inflammatory response and neuronal apoptosis. However, its mechanisms underlying the anti-ischemia/reperfusion (I/R) injury effects on the regulation of small molecule metabolism in rat brain remains unclear. The purpose of this study...
Article
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Dl-3-n-butylphthalide (NBP) has been widely used to treat ischemic stroke in China. To investigate the mechanisms underlying NBP activity, we established a permanent middle cerebral artery occlusion (pMCAO) rat model and injected the rats with 4 mg/kg/d NBP for nine days. We then assessed neuroinflammation, neovascularization and nerve regeneration...
Article
Asthenozoospermia (AS), defined as low-motility spermatozoa in the ejaculate, is a frequent cause of human male infertility. DJ-1 (also known as PARK7), a protein highly associated with male sterility, binds to the mitochondrial complex I subunit to protect mitochondrial function. However, its involvement in spermatogenesis has not been fully eluci...
Article
Full-text available
Safflower (Carthamus tinctorius. L.), a Chinese materia medica, is widely used for the treatment of cardiovascular and cerebrovascular diseases, with flavonoids being the major active components. Multiple flavonoids in safflower bind to Parkinson's disease (PD)-related protein DJ-1. Safflower flavonoid extract (SAFE) improved behavioral indicators...
Article
Full-text available
Ischemic stroke is a syndrome of severe neurological responses that cause neuronal death, damage to the neurovascular unit and inflammation. Notoginsenoside R1 (NG-R1) is a neuroprotective drug that is commonly used to treat neurodegenerative and cerebrovascular diseases. However, its potential mechanisms on the regulation of small molecule metabol...
Article
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The extracellular space (ECS) is the space between the neurons and the capillaries in the brain. The volume fraction (α) and the tortuosity (λ) are the main parameters used to describe its characteristics. Thymoquinone has been proved to possess anti-oxidant and anti-inflammatory activity. In this study, we used a gadolinium-diethylenetriaminepenta...
Article
Full-text available
Parkinson's disease (PD) is a common degenerative disease of the central nervous system, and the pathologic features are mainly degeneration of substantianigra and dopamine neurons. Studies have shown that safflower flavonoid extract (SAFE) exhibits the neuroprotective effect. In this study, the safflower flavonoid extract drop pills (SAFE-DPs) for...
Article
Full-text available
Imaging technologies for the analysis of the central nervous system are rapidly developing. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging, tracer-based magnetic resonance imaging, CLARITY technology and optogenetics can be used to visualize small molecules in brain tissues, the interstitial system of the brain...
Preprint
Full-text available
Background Dl-3-n-butylphthalide (NBP) has been widely used for the treatment of ischemic stroke in China. However, its mechanisms of action have not been fully elucidated. Methods We established a permanent middle cerebral artery occlusion (pMCAO) rat model and administered 4 mg/kg/d NBP by tail vein injection for 9 days. Changes in some molecule...
Article
Full-text available
Methamphetamine (METH) has already been a serious problem all over the world. The identification of related biomarkers and pathways is helpful to evaluate the degree of METH addiction, develop appropriate treatment during abstinence, and explore the mechanism. Here, it is the first time to perform metabolomics profiling of METH addicted human serum...
Article
Full-text available
Objectives: We aimed to evaluate the effect of Shenfu injection in a rat model of ischemic heart failure and explore its mechanism. Methods: A rat model of ischemic heart failure after myocardial infarction was established by ligating the left anterior descending coronary artery. Forty-eight hours after surgery, the rats were intraperitoneally admi...
Article
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Radix Aconiti Lateralis Preparata (fuzi) is the processed product of Aconitum carmichaelii Debeaux tuber, and has great potential anti-myocardial infarction effects, including improving myocardial damage and energy metabolism in rats. However, the effects of Radix Aconiti Lateralis Preparata extracts in a rat model of myocardial infarction have not...
Article
Objective: To comparatively study the toxicity of four metal-containing nanoparticles (MNPs) and their chemical counterparts to the air-blood barrier (ABB) permeability using an in vitro model. Methods: ABB model, which was developed via the co-culturing of A549 and pulmonary capillary endothelium, was exposed to spherical CuO-NPs (divided into...
Article
Ethnopharmacological relevance Shenfu injection is a popular Chinese herbal formula that has been widely used in the treatment of shock in China. Aim of the study To investigate the effect of Shenfu injection on lipopolysaccharide (LPS)-induced septic shock in rabbits. Materials and methods We established a septic shock model in rabbits by admini...
Article
Full-text available
We used Box-Behnken design-based (BBD) response surface methodology (RSM) in this research to optimize the extraction process of Traditional medicine Majun Mupakhi Ela (MME) and evaluate its effect on hydrocortisone-induced kidney yang deficiency. Three independent parameters were applied to evaluate the maximum phosphodiesterase type 5 (PDE5) inhi...
Article
Background: At present, the harm of new-type drug, methamphetamine (METH), has gradually exceeded that of the traditional opioid drugs, and METH abuse has become a serious public health and social problem. In our previous study, complement factor H (CFH) was found to be upregulated in the sera of METH-addicted patients and rats and in certain brai...
Article
Methamphetamine (MA), which has the effect of mental excitement, is one of amphetamine-type stimulants (ATS). It has very strong tolerance and addiction, with high relapse rate, easy access, unconspicuous physical withdrawal symptoms and other characteristics. Because the number of MA addicts is gradually rising in recent years, the research of MA...
Article
Full-text available
Immune thrombocytopenia (ITP) is a common acquired autoimmune disease, and thrombopoietin (TPO) is an important cytokine that regulates the production of megakaryocytes and platelets. We have identified a biologically active component, icaritin, from a Chinese herba epimedii extract. Icaritin promotes platelet production and regulates T cell polari...
Article
Full-text available
Asthenozoospermia (AS), an important cause of male infertility, is characterized by reduced sperm motility. Among the aetiologies of AS, inflammation seems to be the main cause. DJ-1, a conserved protein product of the PARK7 gene, is associated with male infertility and plays a role in oxidative stress and inflammation. Although our previous studie...
Article
DJ-1, originally identified as an oncogene product, is involved in multiple processes, including cellular transformation, the oxidative stress response, and transcriptional regulation. We had previously found that DJ-1 was closely associated with the proliferation and differentiation of cultured neuronal stem cells. In this study, we further invest...
Data
The structure of the compound 1,7-dihydroxy-3,4,8-trimethoxyxanthone (ZYC-1) isolated from Swertia punicea Hemsl. (TIF)
Data
The flowchart of animal experiment. (TIF)
Data
The expression level of DJ-1 protein was detected by Western blot. (TIF)
Data
Protein expression of Bax, Bcl-2, Caspase-3, Caspase-9 and AIF was detected by Western blot. (TIF)
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The detailed MF or BP information of the differentially expressed proteins. (CSV)
Data
Protein expression of α-SMA was detected by Western blot. (TIF)
Article
Full-text available
Chronic cerebral hypoperfusion is a pathological state that is associated with the cognitive impairments in vascular dementia. Oxiracetam is a nootropic drug that is commonly used to treat cognitive deficits of cerebrovascular origins. However, oxiracetam is currently used as a racemic mixture whose effective ingredient has not been identified to d...
Article
Full-text available
Dl-3-n-butylphthalide (NBP) is a drug that is used in the treatment of ischaemic stroke. However, to the best of our knowledge, there are no systematic studies investigating the effects of dl-3-n-butylphtalide on the brain metabolism of small molecules. In this study, we first investigated the effects of dl-3-n-butylphthalide on the spatial distrib...
Article
Methamphetamine (MA), is one of amphetamine-type stimulants(ATS). Compared with traditional opioid drugs, the biggest difference of MA is its strong psychological dependence, and with high relapse rate, easy to get, unconspicuous physical withdrawal symptoms. Its addiction mechanism is complex, involving multiple brain regions, a variety of neurotr...
Article
Full-text available
Platelet-activating factor (PAF) has been shown to play a critical role in mediating vascular hyperpermeability during anaphylaxis. Although epinephrine is the first-line treatment for anaphylaxis and can inhibit increased vascular permeability during anaphylaxis, the effect of epinephrine on PAF-induced vascular hyperpermeability is still unclear....
Article
Asthenozoospermia is a common cause of male infertility and is characterized by reduced forward motility of spermatozoa. The pathogenesis of asthenozoospermia remains unclear. DJ-1 is a ubiquitous protein, widely expressed in the liver, skeletal muscle, kidney, brain and testis. It has been found correlated with many diseases including male inferti...
Article
Immune thrombocytopenia (ITP) is an acquired common hemorrhagic condition characterized by a low platelet count and hemorrhage, which is caused by excessive platelet destruction and thrombopoiesis deficiency. Clinical therapy includes pharmacotherapy with corticosteroids and immunosuppressants and surgical treatment of splenectomy in order to reduc...
Article
Full-text available
Kaempferol-3-O-rutinoside (K3R) has been proven to have biological activities for the prevention and treatment of central nervous system (CNS) diseases. However, the details of its absorption from the gastrointestinal tract and transport across the blood–brain barrier (BBB) are not clear. Therefore, to provide a basis for its efficacy, we explored...
Article
Objective: To investigate effects of anhydroicaritin microemulsion formulation on the immunologic thrombocytopenic purpura (ITP) mice. Methods: Male BALB/c mice were randomly divided into five groups. Constant ITP was induced in four groups by intraperitoneally (ip) injecting guinea pig anti-mouse thrombocyte antiserum with a dose of 100 μL at 1∶4...
Article
Full-text available
Chronic kidney disease (CKD) poses a serious threat to the quality of human life and health with an increasing incidence worldwide. Renal fibrosis is closely related to CKD and regarded as the final common pathophysiological pathway in most cases of end-stage renal diseases. Elucidating the mechanisms underlying renal fibrosis and developing novel...
Article
Carthamus tinctorius L., or safflower, is an annual herbaceous crop belonging to the family Asteraceae, which is cultivated throughout China and used as a traditional Chinese medicine. Our previous study revealed anti-Parkinson's disease effects of an isolated standardized safflower flavonoid extract (SAFE). The purpose of this study is to evaluate...
Article
Full-text available
Parkinson?s disease (PD) is a major age-related neurodegenerative disorder characterized by the loss of dopaminergic neurons in the substantia nigra par compacta (SNpc). Rotenone is a neurotoxin that is routinely used to model PD to aid in understanding the mechanisms of neuronal death. Safflower (Carthamus tinctorius. L.) has long been used to tre...
Article
Full-text available
Safflower has long been used to treat cerebrovascular diseases in China. We previously reported that kaempferol derivatives of safflower can bind DJ-1, a protein associated with Parkinson’s disease (PD), and flavonoid extract of safflower exhibited neuroprotective effects in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of PD....
Article
Mitochondrial dysfunction plays an important role in the process of PD, DJ-1 participates in regulating the function of mitochondria, which has an effect on the protection of mitochondria. DJ-1 mutations can lead to the decrease of the activity of mitochondrial complex I , the decrease of mitochondrial membrane potential and then mitochondrial frag...
Article
Objective: To investigate the protective effect of Salvia miltiorrhiza powder for injection on renal fibrosis induced by unilateral ureteral obstruction (UUO) in rats. Methods: SD rats were randomly divided into groups, and renal fibrosis was induced by UUO surgery except the sham operation control. After the surgery, rats were treated with intrape...
Article
Objective: To determine the effects of Salvia miltiorrhiza injectional powder on isoproterenol-induced myocardial fibrosis in mice. Methods: KM mice were randomly divided into normal, model, positive control and three doses of Salvia miltiorrhiza injectional powder groups. Except for those in normal group, the mice were administered isoproterenol (...
Article
In 2013 December, Ebola case was found again in Africa and spread to Sierra Leone, Liberia, Nigeria and other west African countries in just a few months. So far, the number of the Ebola virus infection is still in multiplication, which has turned into a threat to other countries in Africa and around the world, and attracted great attention of inte...
Article
Several phenylethanoid glycoside derivatives were designed and synthesized. Most of the synthetic compounds showed significant neuroprotective effects, including antioxidative and anti-apoptotic properties. Specifically, target compounds displayed potent effects against various toxicities such as H2O2 and 6-hydroxydopamine (6-OHDA) in PC12 cells. A...
Article
Abstract Parkinson's disease (PD) is one of the most common neurodegenerative disorders, involving progressive loss of the nigro-striatal dopaminergic neurons. Cardinal symptoms including tremors, muscle rigidity, drooping posture, drooping, walking difficulty, and autonomic symptoms appear when a significant number of nigrostriatal dopaminergic ne...
Article
Objective: To determine the protective effect of Danshen powder for injection on rats with alcoholic liver fibrosis.
Article
Objective: To explore the relationship between reactive oxygen species and DJ-1 in ejaculated spermatozoa from Chinese asthenozoospermia patients.
Article
Full-text available
Heroin addiction is a chronic, complex disease, often accompanied by other concomitant disorders, which may encumber effective prevention and treatment. To explore the differences in expression profiles of serum proteins in control and heroin addicts, we used two-dimensional electrophoresis coupled to MALDI-TOF/TOF, and identified 4 proteins of int...
Article
To explore the drainage process of brain interstitial fluid ( ISF) and transport property in interstitial space ( ISS) in rat deep brain tissue by tracer-based magnetic resonance imaging ( MRI) . On the basis of classical diffusion equation in porous media,to establish distribution and clearance model for local drug administration of brain. Methods...
Article
Objective: To determine the in vitro anti-oxidative and neuroprotective effect of phenolic acid compounds (PAc) from Danshen in a cell model of Parkinson's disease (PD). Methods: After exposure to 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPP+), cell viability was detected by MTT assay. The release of lactate dehydrogenase (LDH) was inspecte...
Article
Objective: To investigate the effect of (5-(3-phenyl-propionylamino)-N-(4-ethoxycarbonyl-phenyl)-1H-3-indole carboxamide) (compound T), a de novo synthetic CD38 inhibitor, on pulmonary injury in mice with ozone-induced airway hyperresponsiveness (AHR). Methods: The effects of gradient doses of compound T (12.5, 25 and 50 mg·kg-1) were evaluated in...
Article
Cabozantinib, a new drug for medullary thyroid cancer treatment, is a potent inhibitor of MET, RET, and vascular endothelial factor receptor 2 (VEGFR2) as well as other tyrosine kinases that have been implicated in tumor development and progression. The mechanism, pharmacokinetics, drug interaction, clinical evaluation, and safety of cabozantinib w...
Article
Objective: To explore the potential molecular mechanisms of asthenozoospermia. Methods: One hundred and thirteen semen samples of asthenozoospermia patients and 58 semen samples of healthy volunteers were collected. Then sperm and seminal plasma were separated, and their oxidative damages were evaluated. Results: For the samples of asthenozoospermi...
Article
Objective: To compare the relaxant effects of several kinds of Chinese herbal medicine extracts containing anthocyanins and glycyrrhizic acid in isolated guinea pig trachea. Methods: Effects of 18 extracts on the resting tension, contractile response to histamine (His) and acetylcholine (Ach) were observed in tracheal rings isolated from guinea pig...
Article
The 3-O-demethylswertipunicoside (3-ODS) is extracted from Swertia punicea. Recent study from our laboratory has demonstrated that the 3-ODS protects against oxidative toxicity and apoptosis in PC12 cells (Zhang, S.P., Du, X.G., Pu, X.P., 2010. Biol. Pharm. Bull. 33, 1529-1533). The aim of our study is to further investigate the neuroprotective mec...
Article
Many patients suffer from a frequently occurred disease in clinic, type I hypersensitivity, which might cause death if it were serious. Because available drugs have various kinds of defects, the research and development of new drugs for type I hypersensitivity has been a common theme to international pharmaceutical companies. Focusing on the cellul...
Article
Objective: To observe the neuroprotective effects of DJ-1 protein on 1-methyl-4- phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) induced Parkinson's disease (PD) in mice. Methods: SDS-PAGE and bio-mass spectrometry were used to determine the purity and active sites of DJ-1 protein. DJ-1 protein was then injected into the lateral ventricles of mice. MPT...
Article
Objective: To establish a technical system of the prokaryotic expression and purification of DJ-1 protein for screening polyphenol compounds of traditional Chinese medicine by SPR. Methods: The prokaryotic expression of wild-type (WT) DJ-1 was done by heat shock method, and IPTG induction after the plasmid was transformed into the competent bacteri...
Article
Ipilimumab is a monoclonal antibody against human cytotoxic T lymphocyte antigen 4 (CTLA-4), and has been approved by FDA for treating advanced melanoma in March 2011. It can significantly prolong overall survival of melanoma patients, which has important clinical significance. This article reviewed its pharmacological effects, pharmacokinetics, cl...
Article
Photoacoustic tomography (PAT) is a new biomedical imaging technique. It combines the tissue optical contrast and ultrasonic detection. It has high sensitivity, specificity, and resolution for three-dimensional imaging. The review describes the technology of PAT and its application prospect in neuropharmacology research. PAT provides a powerful too...
Article
Chlorotoxin (ClTx), originally isolated from scorpion venom of Leiurus quinquestriatus, is a 36-amino acid peptide and specifically binds to the brain gliomas and proliferating vascular endothelial cells. In this paper, it was first used to establish the ClTx-modified stealth liposomes (ClTx-LS) encapsulating levodopa (LD) for the targeting drug de...
Article
Protein kinases are important in the pathogeneses and development of various diseases, especially tumor. Kinase inhibitors, targeting protein kinases, garner the attention of drug research and development (R&D) in recent years. Currently, 11 small-molecule kinase inhibitors have been approved; among them 9 are the tyrosine kinase inhibitors. Kinase...
Article
Aim: To investigate the expression of biliverdin-IX β reductase (BVRB) in the mouse MPTP model and rat 6-OHDA model of PD. Methods: High-performance liquid chromatography (HPLC) was used to determine striatal levels of dopamine and its metabolites, and Western blot analysis to detect tyrosine hydroxylase (TH) expression in nigrostriatal tissue was...
Article
Objective: To investigate the antioxidative protective effect of Fx on Aβ25-35-induced injury in PC12 cells. Methods: PC12 cells were treated with Aβ25-35 to establish a cell model of Alzheimer's disease. After pretreated with chemicals Fx, cell viability was analyzed by MTT assay, the activities of SOD and T-AOC in cell culture supernatants were d...
Article
Objective: To investigate the neuroprotective effect of CTE, an extract from traditional Chinese medicine, in a mouse model of Parkinson's disease induced by 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP). Methods: We induced Parkinson's disease model by MPTP (30 mg· kg -1, qd, ig). After pretreatment with high and low doses (50 and 100 mg...
Article
Aim: To establish an ideal two-dimensional Polyacrylamide gel electrophoresis (2-DE) method for serum proteomic research. Methods: The 2-DE experiment of serum was optimized by adjusting the conditions of sample pretreatment and silver nitrate staining. Results: An ideal 2-DE experiment method with higher repetition was established. The number of p...