Wouter Van Hecke

Wouter Van Hecke
Flemish Institute for Technological Research | VITO · Department of Industrial Innovation

dr. ir.

About

38
Publications
4,745
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693
Citations
Citations since 2016
15 Research Items
510 Citations
2016201720182019202020212022020406080
2016201720182019202020212022020406080
2016201720182019202020212022020406080
2016201720182019202020212022020406080

Publications

Publications (38)
Article
Full-text available
The performance of fed-batch and continuous ABE fermentations integrated with organophilic pervaporation was thoroughly tested. Poly(octyl methyl siloxane) (POMS) based pervaporation membranes were applied during fermentation and compared to the performance of polydimethylsiloxane (PDMS) based membranes. The performance of POMS based pervaporation...
Article
Full-text available
The tech­ni­cal feasili­bity of in­te­grat­ing ABE fer­men­ta­tions with organophilic per­va­po­ra­tion has been de­scribed and demon­strated nu­mer­ous times. How­ever, en­gi­neer­ing guide­lines for in­te­gra­tion of per­va­po­ra­tion with fer­men­ta­tion are cur­rently not avail­able. A novel cal­cu­la­tion pro­ce­dure to size per­va­po­ra­tion...
Article
Full-text available
Industrial biotechnology has a potential to tackle harmful CO2 emissions and turn CO2 into a valuable commodity. However, a major technical obstacle in gas fermentations is the limited gas mass transfer rate. Increasing system pressure is a way to increase the driving force for mass transfer. This review presents critical aspects of gas fermentatio...
Article
Full-text available
An engineering foundation is developed in this manuscript to allow the rational design of enzymatic transesterifications integrated with organic–organic pervaporation for the removal of methanol. In the first part, enzyme kinetics are elucidated for the solventless transesterification of two monoterpene alcohols with methyl acetate catalyzed by Nov...
Article
Xylo-oligosaccharides (XOs) from xylans are gaining interest due to their wide use in food, feed and pharmaceutical industry. In this work, the enzymatic production of XOs from beechwood xylan in batch, continuous and semi-continuous enzyme membrane reactor mode was compared. The potential of an inline viscometer for real-time monitoring of the hyd...
Article
Full-text available
Syngas fermentation to biofuels and chemicals is an emerging technology in the biobased economy. Mass transfer is usually limiting the syngas fermentation rate, due to the low aqueous solubilities of the gaseous substrates. Membrane bioreactors, as efficient gas–liquid contactors, are a promising configuration for overcoming this gas-to-liquid mass...
Patent
The present invention relates to stabilizing monomers obtained from mono(meth)acrylated monolignols providing AA (antioxidant activity) to copolymers, and obtained copolymers thereof. It further provides methods for preparing such copolymers, the building blocks and the use thereof in the manufacture of copolymers comprising said stabilizing monome...
Article
This paper gives detailed insight in the solventless synthesis of (meth)acrylate esters catalysed by Novozym 435 with a focus on the quantitative understanding of the reaction kinetics. The (meth)acrylate esters were formed from various (heavy boiling) alcohols by applying methyl (meth)acrylate in excess. Attractive ester titers were obtained (up t...
Article
Furan derivatives represent an interesting set of bio-sourced substrates for esterification reactions. However, the use of lipases for the production of furan containing esters is underexploited. Solvent-free lipase catalyzed esterification of several furan containing chemicals was investigated and remarkably high conversion percentages were obtain...
Patent
The present invention relates to a method for producing products, advantageously solvents, by fermentation, advantageously multi-stage fermentation. The fermentation is complemented with pervaporation as in situ product recovery technology, combined with a multistage condensation of the permeate. The condensates are separately introduced in the dow...
Article
The aim of this work was to investigate the productivity of continuous lactate fermentations at increased cell densities on simulated lignocellulosic hydrolyzate and wheat straw hydrolyzates using Ca(OH)2 suspensions as base. To this end, a semi-automated membrane bioreactor prototype with external tubular membranes was designed and constructed. Lo...
Article
Full-text available
In this study, a simulated lignocellulosic hydrolyzate was used in a continuous two-stage fermentor setup for production of acetone, butanol and ethanol. An organophilic pervaporation unit was coupled to the second fermentor. The dilution rate in the first fermentor was kept constant at 0.109 h(-1), while the dilution rate in the second fermentor w...
Patent
The current invention provides and improved method for hydrolyzing alpha-chlorinated toluene compounds comprising the steps of:—providing an alpha-chlorinated toluene compound of formula (I) wherein at least one of R1, R2 and R3 is CI and wherein (Ra)n designates that the benzene nucleus may have one or more substituents which may be same or differ...
Article
The review presents the state-of-the-art in the applications of in-situ product recovery (ISPR) in whole-cell biotechnology over the last 10 years. It summarizes various ISPR-integrated fermentation processes for the production of a wide spectrum of bio-based products. A critical assessment of the performance of various ISPR concepts with respect t...
Article
The enzymatic production of the vitamin C precursor 2-keto-L-gulonate (2-KLG) from 2,5-diketo-D-gluconate (2,5-DKG) was catalyzed by coupling 2,5-diketo-D-gluconic acid reductase (2,5-0KG reductase) via its coenzyme to glucose dehydrogenase. This bi-enzymatic process shows complicated inhibition patterns caused by reaction products, NADP(+) and NAD...
Article
Glucose dehydrogenase (GDH) is frequently used for the reduction of NAD+ and NADP+ in bench- and industrial-scale syntheses because the coenzyme regenerating system GDH is easy to apply, robust and relatively inexpensive. To optimize the application of this long known coenzyme regeneration system we investigated the commonly applied Bacillus GDH an...
Article
Acetone, butanol and ethanol were produced in a continuous two-stage fermentation integrated with pervaporation using freely suspended cells of C. acetobutylicum ATCC 824. PDMS composite pervaporation membranes were directly coupled to the second fermentor which lead to decreased solvent titers. Overall productivity was increased from 0.45gL(-1)h(-...
Article
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This article has been removed: please see Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy).
Article
Full-text available
This article has been removed: please see Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy).
Article
A continuous cultivation of Clostridium acetobutylicum ATCC 824 is described using a two-stage design to mimic the two phases of batch culture growth of the organism. A hydrophobic pervaporation unit was coupled to the second fermentor containing the highest solvent titers. This in situ product recovery technology efficiently decreased butanol toxi...
Poster
Full-text available
Modelling and upscaling of a biocatalytic cascade oxidation in a dynamic membrane aeration reactor
Article
The oxidation of lactose to lactobionic acid by a bi-enzymatic system in a membrane-aerated reactor was scaled up 100-fold from a miniaturized membrane contactor to a 20-L dynamic membrane aeration reactor. The conversion was catalyzed by an enzyme cascade consisting of cellobiose dehydrogenase as synthesizing enzyme and laccase as regenerating enz...
Poster
Full-text available
Upscaling the biocatalytic conversion of lactose to lactobionic acid with the dynamic membrane aeration reactor
Article
The interactions between two oxidoreductases coupled by an artificial redox mediator have been described quantitatively to increase both stability and productivity. In this cascade oxidation, pyranose 2-oxidase oxidizes several aldoses at the C-2 position to 2-ketoaldoses. A redox mediator is used as electron acceptor for pyranose 2-oxidase because...
Article
A model has been developed to describe the interaction between two enzymes and an intermediary redox mediator. In this bi-enzymatic process, the enzyme cellobiose dehydrogenase oxidizes lactose at the C-1 position of the reducing sugar moiety to lactobionolactone, which spontaneously hydrolyzes to lactobionic acid. 2,2'-Azino-bis(3-ethylbenzothiazo...
Article
The effect of bubble-free oxygenation on the stability of a bi-enzymatic system with redox mediator regeneration for the conversion of lactose to lactobionic acid was investigated in a miniaturized reactor with bubbleless oxygenation. Earlier investigations of this biocatalytic oxidation have shown that the dispersive addition of oxygen can cause s...
Thesis
Manufacturing of fine chemicals traditionally involves oxidations with stoichiometric amounts of oxidants such as hypochlorite, bromine, permanganate, m-chloroperoxybenzoic acid, (trifluoro)peroxyacetic acid and others leading to large amounts of toxic effluents. In contrast, molecular oxygen is the most inexpensive and harmless oxidant availabe. E...
Article
The transport of a gas or vapour through a dense, nonporous membrane can be described in terms of a solution-diffusion mechanism, which states that the permeability is determined by its diffusivity and solubility. The solubility of many different molecules in many different polymers has been measured and can be found in the literature. However, ver...

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Projects

Projects (3)
Project
The goal of this project is to construct a platform for companies with a strategic interest in biocatalysis. In this platform a broad variety of industrially relevant enzymatically catalyzed reactions will be explored. The value chain of the current industrial partners contains a production partner for the initial phase of industrial implementation at intermediate scale, a partner for large scale production and several application partners.