Woranan Nakbanpote

• D Sc (Biotechnology)
• Assoc.Prof. at Mahasarakham University

Gynura pseudochina (L.) DC. is a native Zn and Cd hyperaccumulative plant. It can be applied to rehabilitate an old zinc mine by cooperating with a select Zn/Cd tolerant plant endophyte that contains plant growth-promoting properties. Therefore, this research aims to study the inoculation effects of selected Zn/Cd tolerant endophytic bacteria...

This research aimed to study siderophores secreted from Pseudomonas sp. PDMZnCd2003, a Zn/Cd tolerant bacterium. The effects of Zn and/or Cd stress were examined in nutrient broth to achieve the actual environmental conditions. Acid and alkali supernatants and liquid–liquid extraction with ethyl acetate and butanol were carried out to obtain crude...

This research aims to isolate and identify Zn- and Cd-tolerant endophytic bacteria from Murdannia spectabilis, identify their properties with and without Zn and Cd stress, and to investigate the effect of bacterial inoculation in an in vitro system. Twenty-four isolates could survive on trypticase soya agar (TSA) supplemented with Zn (250–500 mg L⁻...

Gynura pseudochina (L.) DC. is a local herb that has been used as an anti-inflammatory agent. To study the utilization of plant phenolics for psoriatic treatment, the effects of the drying process, solvent extraction process and age of the leaves on the total phenolic content (TPC), total flavonoid content (TFC), crude extract and anti-oxidant acti...

Maize fields near Mae Tao Creek in Pha Te Village, Tak Province, Thailand are contaminated with Zn, Cd, and Pb. This research studied the interaction between levels of the metals contaminating the soil and maize development, heavy metal accumulation in the seeds, and the soil bacterial community structure. Our field experiment was carried out in fi...

Geological survey indicates that Thailand has reserves for gold, silver, potassium, coal, dolomite, gypsum, and quarry mining. Thailand has a long history of mining industry. Classic examples of abandoned mines are as follows: (1) Arsenic contamination at Ron Phibun District and Nakhon Si Thammarat Province, (2) Lead contamination at Klity Creek an...

Gynura pseudochina (L.) DC. is a Zn/Cd hyperaccumulative plant. In an in vivo system under controlled plant age, this research reveals that phenolic compounds and lignification play beneficial roles in protecting G. pseudochina from exposure to an excess of Zn and/or Cd, and Zn reduces Cd toxicity under the dual treatments. The total phenolic conte...

Mining and smelting have a history of centuries. Once nature was predominant and human interference was negligible. Now, due to population explosion and increased demand for materials and energy, pollution from mine waste is glaringly visible. Coal and a variety of minerals are explored in different countries. Opencast mining generates large quanti...

Technogenic and anthropogenic activities are the major sources of heavy metals in the environment. Phytoremediation is the use of plants for environmental cleanup. Economically important plants with a short life cycle motivate local people provided they have phytoremediation application. In this context many ornamental plants have been evaluated fo...

Red yeast rice (RYR) is rice fermented with Monascus. RYR products can be used as food additives, coloring materials, and dietary supplements. Many researchers have reported valuable metabolites in RYR, such as Monascus pigments (red, yellow, and orange) and other bioactive metabolites, especially monacolin K, g-aminobutyric acid (GABA), and citrin...

The entomopathogenic fungus Cod-MK1201 was isolated from a dead cicada nymph. Three regions of ribosomal nuclear DNA, the internal transcribed spacers of nuclear ribosomal DNA repeats (ITS), the partial small subunit of rDNA (nrSSU) , and the partial large subunit of rDNA (nrLSU), and two protein-coding regions, the elongation factor 1α (EF-1α), an...

The history of mining for precious minerals dates back to several centuries. Mining is important for economy but causes environmental contamination. However, mine waste reclamation and mine environment cleanup are a subject of recent origin focusing various aspects of biogeotechnologies. In general, the subject of environmental remediation is about...

Economic development of a nation is linked to richness of natural resources. Minerals are one of the important natural resources. Thailand is rich in minerals such as zinc (Zn), lead (Pb), tin (Sn) and gold (Au). Thailand's largest zinc deposits are located in Phatat Phadaeng sub-district, Mae Sot, Tak. Mining operations generate huge amounts of wa...

The bacteria of PDMCd0501, PDMCd2007, and PDMZnCd2003 were isolated from a Zn/Cd contaminated soil. They were classified as salt-tolerant bacteria in this experiment. The bacteria had indole-3-acetic acids (IAA) production, nitrogen fixation, and phosphate solubilization, under 8% (w/v) NaCl condition. Biochemical test (API 20E) and 16S rDNA sequen...

Murdannia spectabilis (Kurz) Faden was identified as a Zn/Cd hyperaccumulative plant. Leaf surface anatomy of the plant growing in non-contaminated soil (control) and Zn/Cd contaminated soil,was studied and compared by a light micros-copy and scanning electron microscopy combined with Energy-dispersive X-ray spectroscopy (SEM/EDS). The similarities...

Gynura pseudochina (L.) DC. is a zinc (Zn)/cadmium (Cd) hyperaccumulative plant. The aim of this study was to examine the tolerance of G. pseudochina (L.) DC. for Zn and/or Cd accumulation and protein expression. An in vitro tissue culture system was used to control the environment and effects of the microorganisms. Treatments with higher Zn and Cd...

Gynura pseudochina (L.) DC. was a Zn/Cd hyperaccumulative plant growing in a zinc mining, Tak province, Thailand. The mechanism of heavy metals detoxification and tolerance involved with protein expression. The objective of this study was to establish the effect of protein extraction on the protein structure. To control environment, the plant sampl...

Soil and water contamination with chromium is an issue of recent concern in Thailand due to increases in industrial activity. Gynura pseudochina (L.) DC., a chromium tolerance plant, could be employed to address this problem via phytoremediation. To understand the tolerance mechanism, this study investigated the speciation and distribution of chrom...

Bagasse fly ash (BGFA), sawdust fly ash (SDFA) and activated carbon (AC) were investigated for color removal from wastewater from the printing ink industry after coagulation of water-based ink wastewater. Synthetic water-based ink was used to study the adsorption isotherm. The maximum adsorption capacity of BGFA increased with temperatures, having...

Textile wastewater is contaminated by reactive dye causing unattractive levels of wastewater color, high pH and high salt content when discharged into public water systems. Decolorization of textile wastewater by plant, phytoremediation, is an alternative, sustainable method which is suitable for long term operation. Narrow-leaved cattails are one...

Activated carbon was produced from corncob using CO2 for the activation process. This research investigated the effect of the remaining volatile matter in corncob char combined with CO2 activation to improve the pore properties of corncob activated carbon. The pore structure was characterized using the Brunauer–Emmett–Teller (BET) surface area test...

This research aims to study zinc accumulation by G. pseudochina (L) DC., a hyperaccumulative plant found in the zinc mining Padaeng-Industry Co., Tak, Thailand. Growth of G. pseudochina (L) DC was not significantly different when watered with deionised water (control) or zinc solutions with concentrations of 25, 50, 100 and 150 mg l-1. The rate of...

Micro–mesoporous activated carbons were prepared from various agricultural wastes by physical activation. Agricultural wastes such as macadamia nut-shell, corncob, bagasse bottom ash, sawdust fly ash and rice husk fly ash, were optimized and processed to obtain the highest surface area. The effects of the amount of volatile matter in char, the acti...

The oxidation state of chromium in coir pith after Cr(VI) adsorption from aqueous solution was investigated using electron spin resonance (ESR). To elucidate the mechanism of chromium adsorption on coir pith, the adsorption studies of Cr(VI) onto lignin, alpha-cellulose and holocellulose extracted from coir pith were also studied. ESR signals of Cr...

The utilization of wood-shaving bottom ash (WBA) for the removal of Red Reactive 141 (RR141), an azo reactive dye, was investigated. WBA/H(2)O and WBA/H(2)SO(4) were made by treating WBA with water and 0.1M H(2)SO(4), respectively, to increase adsorption capacity. Adsorption of RR141 from reactive dye solution (RDS) and reactive dye wastewater (RDW...

The coir pith was used as an adsorbent for nickel removal because the coir pith contains high lignin (36%) and cellulose (44%). The optimum condition for nickel removal by coir pith in a batch system was solution pH 4–7, adsorbent dosage of 5% (w/v), an equilibrium contact time of 10min and a temperature of 30°C. The nickel adsorption was fitted to...

The removal of Cr(VI) from electroplating wastewater by coir pith was investigated in a fixed-bed column. The experiments were conducted to study the effect of important parameters such as bed depth (40-60cm) and flow rate (10-30ml min(-1)). At 0.05 C(t)/C(0), the breakthrough volume increased as flow rate decreased or a bed depth increased due to...

Narrow-leaved cattail (Typha angustifolia Linn) has been reported as being useful in the removal of textile dyes from industrial sources. This study investigated the possible mechanism for plant avoidance in this wastewater by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). Evidence from XRD showed the precipitation of d...

Narrow-leaved cattails were studied in synthetic reactive dye wastewater (SRDW) under caustic conditions. The effects of the toxic dye were expressed in terms of relative plant growth rate and the appearance of symptoms such as necrosis, and chronic or acute wilting. The dye toxicity was 25.33 mg l(-1) which was close to approximate the concentrati...

Copper (II) ions adsorbed on rice husk (RH), cellulose extracted from rice husk (RH-cellulose), rice husk heated to 300 °C (RHA300) and rice husk heated to 500 °C (RHA500) were investigated in order to understand their sites of adsorption. α-Cellulose and lignin extracted from coirpith were used as references for cellulose and silica components, re...

This research involved the adsorption of synthetic reactive dye wastewater (SRDW) by chitin modified by sodium hypochlorite and original chitin in batch experiments. The comparison of maximum adsorption capacity used the Langmuir model to describe SRDW adsorption onto chitin and modified chitin under a system pH of 11.0. Maximum dye adsorption by c...

This research investigated the phyto-remediation potentials of Cyperus rotundas Linn (Nutgrass) and Axonopus compressus (Sw.) P. Beauv (Carpetgrass) for cadmium removal from cadmium solution andcadmium-zinc contaminated soil. Plants growth in the solution showed that cadmium decreased the relative growth rate of both grasses. However, the amount of...

Coir pith is a by-product from padding used in mattress factories. It contains a high amount of lignin. Therefore, this study investigated the use of coir pith in the removal of hexavalent chromium from electroplating wastewater by varying the parameters, such as the system pH, contact time, adsorbent dosage, and temperature. The maximum removal (9...

Wood shaving fly ash was used as an alternative adsorbent for gold preconcentration from gold slag. The maximum gold adsorption capacity of wood shaving fly ash washed with tap water (WSFW) at 20, 30, 40, and 60 degrees C was 8.68, 7.79, 7.44, and 7.25 mg(Au)/g(adsorbent), respectively, while of activated carbon it was 76.78, 60.95, 56.13, and 51.9...

Chitosan was able to remove the color from synthetic reactive dye wastewater (SRDW) under acidic and caustic conditions. The effect of the initial pH on SRDW indicated that electrostatic interaction occurred between the effective functional groups (amino groups) and the dye under acidic conditions. Moreover, SRDW adsorption under caustic conditions...

Rice husk treated by sodium hydroxide had a potential to be an inexpensive adsorbent for recovery of nickel from electroplating industry. NaOH-treated rice husk could be used in many forms of grain such as no ground (RHG-T), powder < 75 μm (RH75-T) and heated at 500°C (RH500-T). The maximum adsorption capacity of RHG-T, RH75-T and RH500-T were 4.27...

The aim of this research is to recover gold from the slag of jewellery industry by using sawdust fly ash as a low cost adsorbent. The gold was extracted from the slag by cyanidation process. The slag extraction by cyanidation process was higher at feeding O2 gas (30 mg 1 1) than air (8 mg l-1) to 53%. The sawdust fly ash (SDF) used was divided into...

Color removal of an aqueous solution containing water-based inks, a mixture of resins, pigments, and dyes, was investigated by using metal hydroxide sludge, which is a waste from the electroplating industry. High color removal (>93%) was shown within 5 min from 30 mg/L ink solution using 0.3 g of metal hydroxide sludge/50 mL of ink solution, and an...

Chitin was able to adsorb anionic silver-thiosulphate complexes at pH 2.2 within 5 min. The particle size affected adsorption capacity. The maximum adsorption capacity of chitin powder and flake was 4.37 and 3.61 mg Ag/g, respectively. A high concentration of thiosulphate had the strongest influence on silver-thiosulphate adsorption, because it cou...

Chlorella vulgaris and rice husk were selected from microorganisms and agricultural waste, respectively, to create new gold-eluteable adsorbents for adsorption of gold–thiourea complex, and compared with activated carbon. The maximum gold adsorption of heated-immobilised C. vulgaris, heated rice husk, and activated carbon was 10.34, 28.22 and 35.88...

The goal of this research was to develop a new, efficient adsorbent of gold-thiourea complex, [Au(CS(NH2)2]+. In this experiment, rice husk was heated at three different temperature: 300°C, 400°C and 500°C. Rice husk ash heated at 300°C adsorbed gold thiourea complex, whereas rice husk ash heated at 400°C and 500°C did not. The structure of rice hu...