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Wolfram M Brück

Wolfram M Brück
University of Applied Sciences Western Switzerland Valais-Wallis, Sion, Switzerland · Institute of Life Technologies

PhD

About

75
Publications
7,581
Reads
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1,553
Citations
Additional affiliations
September 2014 - present
University of Applied Sciences Western Switzerland, Valais-Wallis
Position
  • Professor (Full)
March 2011 - February 2013
Nestlé Research Center
Position
  • Senior Researcher
March 2011 - August 2014
Letterkenny Institute of Technology
Position
  • Project Manager
Education
January 1999 - December 2002
University of Reading
Field of study
  • Food and Health Microbiology
September 1997 - December 1998
The University of Manchester
Field of study
  • Medical Microbiology / Molecular Microbiology
September 1995 - May 1997
St. Cloud State University
Field of study
  • Microbiology

Publications

Publications (75)
Article
Full-text available
Background: Cronobacter sakazakii is a new emerging foodborne bacterial pathogen associated with severe lethal diseases such as meningitis, necrotizing enterocolitis, and septicemia in infants and neonates. Powdered infant formula milk (PIFM) has been recognized as one of the main transmission vehicles and contaminated sources of this pathogen. Th...
Article
Full-text available
Background: Clostridium perfringens is one of the highest prevailing spore-forming foodborne pathogens, which is widely distributed and causes severe disease and outbreaks in humans and animals. Raw meat and poultry are the main vehicles of this pathogen. In this study, we investigated the prevalence, antibiotic resistance pattern, toxin-encoding...
Article
Full-text available
Shigellosis is one of the major public health concerns in developing and low-income countries caused by four species of Shigella. There is an apparent need to develop rapid, cost-effective, sensitive and specific methods for differentiation of Shigella species to be used in outbreaks and health surveillance systems. We developed a sensitive and spe...
Article
Full-text available
Shigella species, a group of intracellular foodborne pathogens, are the main causes of bacillary dysentery and shigellosis in humans worldwide. It is essential to determine the species of Shigella in outbreaks and food safety surveillance systems. The available immunological and molecular methods for identifying Shigella species are relatively comp...
Article
Full-text available
Objective Species identification of Shigella isolates are so prominent for epidemiological studies and infection prevention strategies. We developed and evaluated RAPD and ERIC-PCR coupled with HRM for differentiation of non-dysenteriae Shigella species as potential alternative methods. After isolation of eighteen Shigella strains from faecal speci...
Article
Full-text available
The emergence of multi-drug resistant E. coli is an important matter of increasing considerable concern to global public health. The aim of this study was to investigate the incidence, antibiotic resistance pattern and phylogroups of E. coli isolates obtained from raw milk, vegetable salad and ground meat samples collected from Qazvin Province (Ira...
Article
Full-text available
Escherichia coli are remarkably versatile microorganisms and important members of the normal intestinal microbiota of humans and animals. This harmless commensal organism can acquire a mixture of comprehensive mobile genetic elements that contain genes encoding virulence factors, becoming an emerging human pathogen capable of causing a broad spectr...
Article
Full-text available
The emergence of multidrug-resistant Shigella is a significant threat to global public health. Limited studies have investigated the incidence, antimicrobial susceptibility, and genetic diversity of Shigella isolated from food products. Conventional culture-based, serologic, molecular, disk diffusion, PCR, and RAPD-PCR methods were used to determin...
Preprint
Escherichia coli are remarkably versatile microorganisms and important members of the normal intestinal microbiota of humans and animals. This harmless commensal organism can acquire a mixture of comprehensive mobile genetic elements that contain genes encoding viru-lence factors, becoming an emerging human pathogen capable of causing a broad spect...
Preprint
The emergence of multi-drug resistant E. coli is an important matter of increasing considerable concern to global public health. The aim of this study was to investigate the incidence, antibiotic resistance pattern, phylogroups and genetic variation of E. coli isolates from raw milk, vegetable salad and ground meat samples. Methods: Culture-based t...
Preprint
Full-text available
Objective: Gelatin is a byproduct of meat industry and its hydrolysates showed several functionalities such as antioxidant activity. Response surface methodology (RSM) is a statistical method to mode and optimize biological processes. The purpose of this study was to describe and optimize the enzymatic hydrolysis conditions including time, temperat...
Article
Full-text available
Chryseobacterium indologenes is an opportunistic pathogen isolated from human infections and, rarely, from some aquatic animals. A 3-year-old male ball python (Python regius) was admitted to the veterinary clinic by a pet owner because of acute respiratory and swallowing failure. During physical examinations, oral secretions and abscesses were obse...
Chapter
Chitin is the first polysaccharide identified by man. Chitin and its numerous oligomeric and monomeric, acetylated or deacetylated derivates have many physiological functions and applications. Chitin is found in the cuticles of arthropods and is a major constituent of cell walls from fungal, yeast and algae, from where chitin can be extracted chemi...
Chapter
Chitin is the second most abundant biopolymer on earth, next to plant-derived celluloses. It can be found in fungi, insects, and crustacean shells. The processing of crustaceans (e.g., shrimps and crabs) in the EU alone results in more than 100,000 tons of shell waste each year. Chemically, chitin is distinguished from cellulose just by an addition...
Chapter
Breast milk provides optimal species-specific nutrition for the growing animal. Consequently, bovine milk–based nutrition for infants is incapable of providing a balanced nutritional profile without considerable adaptation. Human milk, e.g., has a larger proportion of alpha-lactalbumin (28%) than bovine milk (3%) or whey-dominant formula where β-la...
Article
The use of insects as a source of protein is becoming an important factor for feeding an increasing population. After protein extraction for food use, the insect exoskeleton may offer the possibility for the production of added value products. Here, the aim was to isolate bacteria from the surface of farmed mealworms (Tenebrio molitor Linnaeus, 175...
Article
Chitinous material was extracted from brown crab (Cancer pagarus) shell waste using traditional chemical methods as well as microbial fermentation for life cycle analysis (LCA) and to compare differences in recovery levels and quality. Chemical extraction used 1 mol l–1 hydrochloric acid for demineralization and 1 mol l–1 sodium hydroxide for depro...
Article
Full-text available
The gut of the human neonate is colonized rapidly after birth from an early sparse and highly distinct microbiota to a more adult-like and convergent state, within 1 to 3 years. The progression of colonizing bacterial species is non-random. During the first months of life several shifts commonly occur in the species prevalent in our guts. Although...
Article
Full-text available
The gut of the human neonate is colonized rapidly after birth from an early sparse and highly distinct microbiota to a more adult-like and convergent state, within 1 to 3 years. The progression of colonizing bacterial species is non-random. During the first months of life several shifts commonly occur in the species prevalent in our guts. Although...
Article
Urinary tract infection (UTI) is one of the most prevalent infections in humans. In ≥80% of cases, the etiologic agents are strains of uropathogenic Escherichia coli (UPEC), which commonly reside in the gastrointestinal tract. Lactobacilli have been shown to prevent UTI reoccurrence by restoring the urogenital microbiota when administered vaginally...
Article
Chitin ist eines der am häufigsten vorkommenden Biopolymere der Erde. Große Mengen dieses Rohstoffes können aus Schalenresten der Fischerei-Industrie gewonnen werden. Allerdings sind die etablierten chemischen Technologien mit hohen Umweltbelastungen verbunden. Die mikrobiologische Fermentation und der Einsatz von Enzymen zur Gewinnung des Polymers...
Article
From crustacean to cream Chitin is one of the most abundant biopolymers on earth and can be obtained in large quantities from shell waste from the fishing industry. However, established chemical technologies are hazardous to human health and the environment due to the use of highly acidic and alkali conditions. Microbiological fermentation and the...
Article
In this study, a duplex qPCR assay was developed for the needs of the Irish fish industry to screen for the two major food-borne pathogens of fish, Listeria monocytogenes and Escherichia coli O157:H7. The assay can claim positive or negative results for two pathogens in one go in only 20 hours including 16 hour universal pre-enrichment and compared...
Article
Full-text available
We found that the relatively simple microbiota of young infants shifts predictably to a more mature anaerobic microbiota during infancy and the dynamics of this shift are influenced by environmental factors. In this longitudinal study of 75 infants, we demonstrate high interindividual variability within the normal range of birth outcomes, especiall...
Article
AimsTo isolate bacteria from soil for the purpose of microbial pre-treatment of brown crab (Cancer pagurus) shell waste and the production of chitin.Methods and ResultsIsolates were screened for protease enzymes and acid production in order to facilitate the removal of protein and calcium carbonate fractions from brown crab shell to yield a chitino...
Article
α-Lactalbumin (α-la) is a major whey protein found in milk. Previous data suggested that α-la has antiproliferative effects in human adenocarcinoma cell lines such as Caco-2 and HT-29. However, the cell death inducing α-la was not a naturally occurring monomer but either a multimeric variant or an α-la:oleic acid complex (HAMLET/BAMLET). Proteolysi...
Chapter
The marine environment is a diverse source of compounds which, through the use of biotechnology, yield a great variety of new products for industrial development. To date, thousands of unique chemical compounds for use in pharmaceuticals, cosmetics, nutritional supplements, molecular probes, enzymes, fine chemicals, and agrichemicals have been iden...
Article
Full-text available
The marine lithistid sponge Discodermia spp. (Family Theonellidae) contains many types of associated bacteria visible in the mesohyl while biofilms cover the pinacoderm. This study determined the identity of bacteria associated with members of the genus Discodermia using microbial culture, 16S rRNA gene clone libraries and fluorescence in situ hybr...
Article
Full-text available
Marine sediments and sponges may show steep variations in redox potential, providing niches for both aerobic and anaerobic microorganisms. Geodia spp. and sediment specimens from the Straits of Florida were fixed using paraformaldehyde and 95% ethanol (v/v) for fluorescence in situ hybridization (FISH). In addition, homogenates of sponge and sedime...
Article
Full-text available
We recently reported that the biosynthesis of fuscol, a diterpene from the octocoral Eunicea fusca, is inducible by the application of plant signaling factors such as salicylic acid to the coral's algal symbiont. In this study, an mRNA differential display approach has been employed with the dinoflagellate symbiont of this octocoral which has led t...
Article
Legal restrictions, high costs and environmental problems regarding the disposal of marine processing wastes have led to amplified interest in biotechnology research concerning the identification and extraction of additional high grade, low-volume by-products produced from shellfish waste treatments. Shellfish waste consisting of crustacean exoskel...
Article
Due to their unique chemical characteristics (including biodegradability to non-toxic products, physiological inertness and hydrophilicity), chitin, chitosan and their derivatives may be expansively utilized in the biotechnological, agricultural, food protection and nutraceutical, medicinal and pharmacological fields and in the areas of bioremediat...
Article
Full-text available
The lithistid sponge Discodermia dissoluta (family Theonellidae), is found in deep-waters throughout the Caribbean sea and is the source of discodermolide, a natural product with potential anticancer properties, and other secondary metabolites. As with other sponges, large numbers of microbes are harbored in the sponge mesohyl. The microbial popula...
Article
Full-text available
This study examined the symbiotic microbiota of the hexacoral Cirrhipathes lutkeni using traditional plate culture, fluorescence in situ hybridization (FISH) and 16S rDNA characterization. FISH counts for the whole coral (holobiont) showed a major presence of gamma-Proteobacteria (22%) and Actinobacteria (19%), followed by alpha-Proteobacteria (14%...
Article
Full-text available
This study examined the microbiota associated with the marine azooxanthellate octocorals Leptogorgia minimata, Swiftia exertia, and Iciligorgia schrammi collected from moderate depths (45 m). Traditional aerobic plate culture, fluorescence in situ hybridization (FISH), and molecular identification of the 16S rDNA region were used for this purpose....
Article
Certain milk factors may promote the growth of a host-friendly gastrointestinal microbiota, for example, one that is predominated by bifidobacteria, a perceived health-promoting genus. This may explain why breast-fed infants experience fewer intestinal infections than their formula-fed counterparts who are believed to have a more diverse microbiota...
Article
Sponges are the dominant organisms on many coral reefs and through feeding they may greatly reduce the concentration of suspended food particles. Retention efficiencies of the tubular sponges Aplysina lacunosa, Callyspongia vaginalis and Niphates digitalis were examined on a coral reef located in the Florida Keys. Replicate ambient and exhalant wat...
Article
Two milk components, alpha-lactalbumin (alpha-La) and glycomacropeptide (GMP) may inhibit intestinal infection/intoxification. (3)[H] thymidine-labeled enteropathogenic Escherichia coli (EPEC), Salmonella typhimurium (ATCC 6994) or Shigella flexneri (ATCC 9199) were introduced to CaCo-2 cultures and their association with CaCo-2 cells was assessed....
Article
Full-text available
There is increasing awareness that the human gut microflora plays a critical role in maintaining host health, both within the gastrointestinal tract and, through the absorption of metabolites, systemically. An "optimal" gut microflora establishes an efficient barrier to the invasion and colonisation of the gut by pathogenic bacteria, produces a ran...
Article
Certain milk factors may help to promote the growth of a host-friendly colonic microflora (e.g. bifidobacteria, lactobacilli) and explain why breast-fed infants experience fewer and milder intestinal infections than those who are formula-fed. The effects of supplementation of formula with two such milk factors was investigated in this study. Infant...
Article
Objectives: Certain milk factors may help to promote the growth of a host-friendly colonic microflora (e.g. bifidobacteria, lactobacilli) and explain why breast-fed infants experience fewer and milder intestinal infections than those who are formula-fed. The effects of supplementation of formula with two such milk factors was investigated in this s...
Article
Certain milk factors may promote the growth of a gastrointestinal microflora predominated by bifidobacteria and may aid in overcoming enteric infections. This may explain why breast-fed infants experience fewer intestinal infections than their formula-fed counterparts. The effect of formula supplementation with two such factors was investigated in...
Article
Certain milk factors can promote the growth of a host-friendly gastrointestinal microflora. This may explain why breast-fed infants experience fewer intestinal infections than their formula-fed counterparts. The effect of formula supplementation with two such factors was investigated in this study. Infant faecal specimens were used to ferment formu...
Article
The two most commonly used targets for diagnosis of pertussis by the polymerase chain reaction have been the pertussis toxin promoter and the repeated insertion sequence IS481. A comparative assessment of these primers was performed on routinely collected nasopharyngeal swabs, stored at -20 C, using novel semiquantitative enzyme immunoassays. Both...

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