Wojciech Mikołaj Śmigiel

University of Groningen | RUG · Membrane Enzymology Group

The human pathogen Listeria monocytogenes can cope with severe environmental challenges, for which the high molecular weight stressosome complex acts as the sensing hub in a complicated signal transduction pathway. Here, we show the dynamics and functional roles of the stressosome protein RsbR1 and its paralogue, the blue-light receptor RsbL, using...

We analyze the structure of the cytoplasm by performing single-molecule displacement mapping on a diverse set of native cytoplasmic proteins in exponentially growing Escherichia coli. We evaluate the method for application in small compartments and find that confining effects of the cell membrane affect the diffusion maps. Our analysis reveals that...

The human pathogen Listeria monocytogenes is a model microorganism in infection biology. The organism can transform from a saprophyte to an intracellular pathogen and cope with severe environmental challenges, for which the high molecular weight stressosome complex acts as the sensing hub in a complex signal transduction pathway. Yet, little is kno...

One of the grand challenges in chemistry is the construction of functional out-of-equilibrium networks, which are typical of living cells. Building such a system from molecular components requires control over the formation and degradation of the interacting chemicals and homeostasis of the internal physical-chemical conditions. The provision and c...

The bottom-up construction of synthetic cells from molecular components is arguably one of the most challenging areas of research in the life sciences. We review the impact of confining biological systems in synthetic vesicles. Complex cell-like systems require control of the internal pH, ionic strength, (macro)molecular crowding, redox state and m...

One of the grand challenges in chemistry is the construction of functional out-of-equilibrium networks, which are typical of living cells. Building such a system from molecular components requires control over the formation and degradation of the interacting chemicals and homeostasis of the internal physical-chemical conditions. The provision and c...

Free fraction of GFP variants in E. coli, L. lactis and Hfx. volcanii.

GFP sequences and diffusion coefficients (A) Amino acid sequences of the GFP variants. (B) Diffusion coefficients in E. coli, L. lactis and Hfx. volcanii. (C) P-values for pairwise comparisons of diffusion coefficients for GFP variants in E. coli (Eco), L. lactis (Lla), and Hfx. volcanii (Hvo). For E. coli we also compared diffusion coefficients un...

Much of the molecular motion in the cytoplasm is diffusive, which possibly limits the tempo of processes. We studied the dependence of protein mobility on protein surface properties and ionic strength. We used surface-modified fluorescent proteins (FPs) and determined their translational diffusion coefficients (D) in the cytoplasm of Escherichia co...

https://doi.org/10.1016/j.bpj.2016.11.1311