Wojciech Maria Kwiatek

Wojciech Maria Kwiatek
Institute of Nuclear Physics · Division of Interdisciplinary Research

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About

217
Publications
37,718
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2,396
Citations
Additional affiliations
January 2016 - October 2016
Institute of Nuclear Physics
Position
  • Head of Department
Education
October 1976 - February 1981
Jagiellonian University
Field of study
  • Physics

Publications

Publications (217)
Article
Metal nanoparticles, with their unique physicochemical and biological properties, have become increasingly studied for application in the medical field. However, the observed cytotoxicity of these structures is still a concerning issue. One of the factors determining the toxic effect of nanoparticles is the stabilizer that is present on the nanopar...
Article
The paper concentrates on the role of saliva in the early diagnosis of salivary gland tumor. Due to the still not fully understood etiology of head and neck cancer, the fundamental objective of this study is to find tumor markers of salivary gland tumor progression. This work demonstrates the feasibility of Attenuated Total Reflection Fourier Trans...
Article
The family of vibrational spectroscopic imaging techniques grows every few years and there is a need to compare and contrast new modalities with the better understood ones, especially in the case of demanding biological samples. Three vibrational spectroscopy techniques (High Definition FT‐IR, Raman and AFM‐IR) were applied for subcellular chemical...
Article
Full-text available
The proper diagnosis is a critical factor to reduce further disturbances at the early stage of the disease and plays an important role in increasing the success rate of the therapy. The traditional diagnostic tools such as biopsy or blood collection are always associated with patient’s discomfort, the possibility of infections and time-consuming pr...
Article
High performance carbon nanotube fibres and yarns with similar strength to Kevlar could now offer significant structural reinforcement and multifunctionality to 3D printed parts. However, recently printed parts which were reinforced with highly-densified CNT yarns exhibited only modest mechanical properties due to poor wettability with polymers. In...
Article
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In this study, surface - enhanced Raman spectroscopy (SERS) was applied at the first time for estimation of how pH, temperature, and nanoparticle (NP) stabilizer affect an adsorption behavior of erlotinib (drug approved in a non-small cell lung cancer therapy) onto citrate-stabilized silver nanoparticles (AgNPs). Novel approach to improve cancer th...
Article
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A noise-free hyperspectral FT-IR imaging dataset of a pancreatic tissue core was simulated based on experimental data that allows to test the performance of various data analysis and processing algorithms. A set of experimental noise levels was also added and used for denoising approaches comparison, which due to the noise-free reference signal ena...
Article
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The integrity of the chromatin structure is essential to every process occurring within eukaryotic nuclei. However, there are no reliable tools to decipher the molecular composition of metaphase chromosomes. Here, we have applied infrared nanospectroscopy (AFM-IR) to demonstrate molecular difference between eu- and heterochromatin and generate infr...
Article
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Correction for ‘Mid-infrared spectroscopy and microscopy of subcellular structures in eukaryotic cells with atomic force microscopy – infrared spectroscopy’ by Luca Quaroni et al. , RSC Adv. , 2018, 8 , 2786–2794.
Article
The work presents a detailed analysis of adsorption geometry of nocodazole, an antineoplastic agent, deposited onto gold nanoparticle surface. This type of study is fundamental because of improving drugs delivery specifically to cancer cells. Simultaneously, it will revolutionize oncology, dramatically improving the effect of drugs while reducing o...
Article
Full-text available
In this study, we provide the first Fourier-transform infrared absorption spectroscopy (FTIR) and Raman spectroscopy (RS) analysis of a vibrational fingerprint of erlotinib, a drug which is applied in non-small cell lung cancer therapy, in solid state and solution in different pH conditions. Additionally, the performed DFT theoretical calculations...
Article
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The recent development of AFM-IR technique, which combines nanoscale imaging with chemical contrast through infrared spectroscopy, opened up new fields for exploration, which were out of reach for other modalities, e.g. Raman spectroscopy. Lipid droplets (LDs) are key organelles, which are associated with stress response mechanisms in cells and the...
Article
Owing to the high information content about the biochemical composition of the sample, the implementation of Fourier-Transform Infrared Spectroscopy (FT-IR) in the clinic is currently under investigation by many researchers. Cancer biology with the use of histopathological models is one of the most explored application areas. Most of the publicatio...
Article
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Understanding the response of cancer cells to ionising radiation is a crucial step in modern radiotherapy. Raman microspectroscopy, together with Partial Least Squares Regression (PLSR) analysis has been shown to be a powerful tool for monitoring biochemical changes of irradiated cells on the subcellular level. However, to date, the majority of Ram...
Article
For incident x-ray energies below the core level ionization threshold, one-photon absorption (OPA) leads to off-resonant x-ray excitations that are mediated by a virtual intermediate electronic state. In the linear regime of x-ray interaction, this intermediate state may decay radiatively and the maximum energy of the emitted photon is given by the...
Article
Attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) and optical microscopy were applied to identify pigments, binders, fillers and degradation products in the 17th century painting 'The Coronation and Assumption of the Blessed Virgin Mary’. This piece of art is located in the Convent of the Sisters of St. Catherine in Orn...
Article
Human prion protein is a subject of extensive study, related in particular to molecular basis of neurodegenerative disease development and prevention. This protein has two main domains: membrane C-terminal, structured domain as well as unstructured N-terminal domain. While PrPC (23-231) has up to eight Cu(II) binding sites in N-terminal domain, it...
Article
Biological membranes are undoubtedly very interesting systems. Unfortunately, their analysis is very complicated and therefore simplified artificial models, like Langmuir monolayers, are frequently applied. In this work a novel method of label-free monomolecular films analysis based on infrared nano-spectroscopy (AFM-IR) is presented. In order to v...
Article
Raman spectroscopy was applied to the measurement of urinary and in vitro endothelium-derived extracellular vesicles (EVs) isolated by hydrostatic filtration dialysis (HFD) method. Raman spectra obtained for urinary EVs (UEVs) showed distinct differences in the fingerprint region. In contrast, average Raman spectra of endothelium-derived EVs sample...
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Many transition metal ions modulate the aggregation of different amyloid peptides. Substoichiometric zinc concentrations can inhibit aggregation, while an excess of zinc can accelerate the formation of cytotoxic fibrils. In this study, we report the fibrillization of the octarepeat domain to amyloid-like structures. Interestingly, this self-assembl...
Article
Currently, there is great interest in bringing the application of IR spectroscopy into the clinic. This however will require a significant reduction in measurement time as Fourier Transform Infrared (FT-IR) imaging takes hours to days to scan a clinically relevant specimen. A potential remedy for this issue is the use of Quantum Cascade Laser Infra...
Article
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This research presents a multianalytical approach to the surface-enhanced infrared absorption spectroscopy (SEIRA) and tip-enhanced infrared nanospectroscopy (TEIRA) studies of α-methyl–DL–tryptophan adsorption geometry on a gold nanoparticle surface. Comparison the SEIRA spectra collected in the transmission, attenuated total reflection and reflec...
Article
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The recent emergence of High Definition (HD) FT-IR and Quantum Cascade Laser (QCL) Microscopes elevated the IR imaging field very close to clinical timescales. However, the speed of acquisition and data quality are still the critical factors in reaching the clinic. Denoising offers aide in both aspects if performed properly. However, there is a lac...
Article
Raman spectroscopy of erythrocytes provides detailed information about the structure and status of heme moiety, which can be used to provide new insights into molecular pathogenesis of several diseases. In this study, we present the first Raman spectroscopy investigations of the effect of hemoglobin oxygenation in the context of hypertensive diseas...
Article
Herein we report on the hydrolysis mechanism of [Pt{N(p-HC6F4)CH2}2(NC5H5)2(OH)2], a platinum(IV) complex that exhibits anti-cancer properties. Atomic telemetry, an in situ technique based on electron structure sensitive X-ray spectroscopy, revealed that hydrolysis preceded any reduction of the metal center. The obtained results are complemented wi...
Article
Exposure to ionising radiation can induce cellular defence mechanisms including cell activation, rapid proliferation prior to metastasis and in extreme cases can result in cell death. Herewith we apply infrared nano- and micro-spectroscopy combined with multidimensional data analysis to characterise the effect of ionising radiation on single gliobl...
Article
In this study, we present surface‐enhanced Raman spectroscopy (SERS) investigations for the erlotinib adsorbed onto silver nanoparticles (AgNPs). The observed spectral patterns, namely, changes in width and wavenumber of the SERS bands in comparison with these occurring in the corresponding Raman spectrum suggest how the investigated drug interacts...
Article
Objectives: To assess the relationships of clinical and laboratory parameters, with erythrocyte oxygen concentration. Methods: The study group consisted of 47 healthy adults out of which 16 showed slightly higher blood pressure and were classified as individuals with prehypertension. For each individual, data were obtained on: systolic and diast...
Article
Full-text available
We have introduced a novel approach that combines the conventional infrared spectroscopy (IR) and atomic force microscopy (AFM) to better understanding the behavior of the drug adsorbed on a metal substrate at the nanoscale level. The socalled tip-enhanced infrared nanospectroscopy (TEIRA) was used for the first time to investigate Lu AA33810, a se...
Article
Lightweight nanocomposites reinforced with carbon nanotube (CNT) assemblies raise the prospects for a range of high-tech engineering applications. However, a correlation between their heterogeneous chemical structure and spatial organization of nanotubes should be clearly understood to maximize their performance. Here, we implement the advanced ima...
Conference Paper
Atomic Force Microscopy-Infrared (AFM-IR) spectroscopy allows spectroscopic studies in the mid-infrared spectral region with a spatial resolution better than 50 nm. [1] The high spatial resolution allows in principle to perform studies of eukaryotic cells with subcellular and single-organelle resolution. We now use fixed fibroblast cells to demonst...
Article
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Preliminary studies on the sediments collected from water meters of Krakow water supply system were performed in the cooperation with the Municipal Water Supply and Sewage. Creation and deposition of sediments on the measuring devices installed in the water supply system is a serious technological and economical problem for water companies, defecti...
Article
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In this study, the Fourier-transform infrared absorption and the Raman spectroscopies for analysis of corrosion products formed on the Fe and Cu metal surfaces after deposition in the chloride containing solution, were used. The obtained spectral patterns show that main constituent species of the corrosion products for Fe metal sample is lepidocroc...
Article
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While studying the influence of ionizing radiation or certain chemical agents on cells, it is crucial to not only determine cytotoxicity, but also to follow cell death mechanisms. There are di erent methods to screen processes of cell death and still very important question remains unanswered about di erences in results that could be caused by vari...
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Objective: The goal of this contribution is to present and familiarize the medical community with the method for the assessment of trace and essentials elements in prostate tissue sections. Materials and methods: X-ray fluorescence based technique(namely Synchrotron Induced X-ray Emission (SRIXE)) is described in terms of methodology, sample pre...
Article
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An investigation of a numismatic collection of silver denarii from the early Piast dynasty was conducted using Fourier-transform infrared spectroscopy. The studied coins, minted between 995 and 1020 ad under the rules of Boleslaus the Brave and Mieszko II Lambert, belong to the collection of the National Museum in Kraków. Fourier-transform infrared...
Poster
Modern technique combining infra-red spectroscopy and atomic force microscopy (AFM-IR) allows the chemical mapping with the spatial resolution down to 30 nm. However, due to specific detection of the infrared signal special attention must be paid to data-processing stage. The obtained results were carefully evaluated with the help of open source so...
Article
This report describes the first detailed characterization of the molecular structure of Lu AA33810, a selective Y5 receptor antagonist, and its behavior at the solid/liquid interface after conjugation with gold nanoparticles (GNPs). Physicochemical characterization, including imaging by scanning electron microscopy (SEM) as well as electrophoretic...
Article
Aortic valve is a part of the heart most frequently affected by pathological processes in humans what constitute a very serious health problem. Therefore, studies of morphology and molecular microstructure of the AV are needed. µSR- FTIR spectroscopy and microscopy represent unique tools to study chemical composition of the tissue and to identify s...
Article
In this study we present vibrational analysis of healthy (non-affected by cataract) and cataractous human lenses by means of Raman and FTIR spectroscopy methods. The performed analysis provides complex information about the secondary structure of the proteins and conformational changes of the amino acid residues due to the formation of opacificatio...
Conference Paper
In this work, we present the first-of-this-kind analysis of the chemical structure of CNT fibre-reinforced nanocomposites by means of AFM-IR spectroscopy. The morphology and polymer distribution within the infiltrated CNT fibre could be unravelled with sub-40 nm spatial resolution, including nanoscale chemical imaging of the polymer interphase form...
Article
Resonant X-ray emission spectroscopy (RXES) technique was applied to probe electronic states of three Fe compounds: Fe, α-Fe2O3 (hematite) and Fe(NO3)3 × 9 H2O (ferric nitrate) around Fe K-absorption edge with simultaneous detection of Kβ and valence-to-core transitions. We show that deep insights on the valence and conduction band position, such a...
Article
Neurodegenerative diseases are one of the malfunctions of human nervous system, being a class of complex and prominent pathologies. The human prion Protease Resistant Protein (PrP) is protein regulating copper metabolism in mammalian cells through binding of Cu(II) ions to specific fragments. Nowadays misfolding of this protein is associated with d...
Article
Full-text available
Atomic Force Microscopy - Infrared (AFM-IR) spectroscopy allows spectroscopic studies in the mid-infrared spectral region with a spatial resolution better than 50 nm. We show that the high spatial resolution can be used to perform spectroscopic and imaging studies at the subcellular level in fixed eukaryotic cells. We collect AFM-IR images of subce...
Article
Full-text available
Spore-forming bacteria are a class of microorganisms that possess the ability to survive in extreme environmental conditions. Morphological features of spores assure their resistance to stress factors such as high temperature, radiation, disinfectants, and drying. Consequently, spore elimination in industrial and medical environments is very challe...
Article
The article describes an X-ray microbeam standalone facility dedicated for irradiation of living cultured cells. The article can serve as an advice for such facilities construction, as it begins from engineering details, through mathematical modeling and experimental procedures, ending up with preliminary experimental results and conclusions. The p...
Book
Full-text available
Book of extended abstracts of papers presented at the international conference ACSIN 2016 held in Frascati, Rome, Italy October 9-15, 2016 Organized by the Non profit organization for scientific research Superstripes onlus, Rome International Center for Materials Science Superstripes - RICMASS. Conference chairs Antonio Bianconi - RICMASS Rome, Ita...
Article
Trace elements concentrations of Ca, Fe, and Zn in cataractous human lenses have been determined using the proton induced X-ray emission technique (PIXE). The technique was selected due to its sensitivity and only modest requirements for sample material processing and preparation. In the study a statistically significant sample of post–surgery (pha...
Article
Lactose and saccharose have the same molecular formula; however, the arrangement of their atoms is different. A major difference between lactose and saccharose with regard to digestion and processing is that it is not uncommon for individuals to be lactose intolerant (around two thirds of the population has a limited ability to digest lactose after...
Article
The biological influence of radiation on living matter has been studied for years; however, several questions about the detailed mechanism of radiation damage formation remain largely unanswered. Among all biomolecules exposed to radiation, DNA plays an important role because any damage to its molecular structure can affect the whole cell and may l...
Article
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A cataract is an opacity (clouding) of the normally clear lens which develops as a result of aging, metabolic disorders, trauma or heredity. The number of patients with cataract is increasing exponentially. This disease requires surgical intervention, to remove the cloudy lens and to introduce the eye lens polymer. In this work we will present anal...
Article
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The aim of this study was to find out if polylactide (PLA) modified with magnetite might affect the amount of DNA double strand breaks induced with X-rays. The human osteosarcoma cells (MG63) were seeded on the polystyrene cell culture dishes (PS), PLA and PLA modified with magnetite substrates. The double strand breaks were analyzed after X-ray ir...
Article
Particle radiotherapy such as protons provides a new promising treatment modality to cancer. However, studies on its efficacy and risks are relatively sparse. Using the cytokinesis-blocked micronucleus assay, we characterized response of human peripheral blood lymphocytes, obtained from health donors irradiated in vitro in the dose range: 0–4. 0 Gy...
Article
Full-text available
The use of synchrotron radiation may shed more light on the study of prostate cancer, one of the leading diseases among men. In the presented study the microbeam setup at the PSI Swiss Light Source combined with fluorescence detected X-ray absorption spectroscopy (XAS) was applied to determine two-dimensional (2D) imaging of distributions of variou...
Article
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A desk-top laser-produced plasma (LPP) source of soft X-rays (SXR) has been developed for radiobiology research. The source is based on a double-stream gas puff target, irradiated with the focused beam of a commercial Nd:YAG laser. The source has been optimized to get a maximum photon emission from LPP in the X-ray "water window" spectral wavelengt...
Article
Synchrotron radiation-Fourier transform infrared (SR-FTIR) microscopy coupled with multivariate data analysis was used as an independent modality to monitor the cellular bystander effect. Single, living prostate cancer PC-3 cells were irradiated with various numbers of protons, ranging from 50-2,000, with an energy of either 1 or 2 MeV using a prot...