Wojciech Krzyczkowski

• PhD
• CTO at Fungotech sp. z o.o.

Nigella sativa L. is cultivated in many regions and its seeds have found use in variety of foods, but also in traditional medicine due to high content of biologically active essential oils. In this work optimization of supercritical carbon dioxide extraction from N. sativa seeds was performed using response surface methodology to describe the influ...

Herbal extracts are promising immunomodulating compounds. Their standardization may improve clinical outcome in various conditions related to inflammatory state. The aim of this study was to assess the utility of Cannabis sativa L. and Humulus lupulus extracts obtained by supercritical carbon dioxide (scCO2) in the reduction of pro-inflammatory cyt...

The object of the invention is the plant composition of anti-inflammatory, anti-allergic, anti-asthmatic and/or anti-bacterial properties, which contains a combination of extracts demonstrating synergistic effects, obtained from the following plant material: the seed of Black cumin Nigella sativa, the root of Garden angelica Angelica archangelica,...

The invention concerns a method of extracting polyphenols, particularly anthocyanins, from plant material, where the process consists of the following stages: a) the plant material is subject to extraction for 1-360 minutes using an extraction solution consisting of a buffer and polyol; b) the solid substances are separated from the raw extract; c)...

Lion’s Mane Mushroom (Hericium erinaceum) is an edible mushroom that contains compounds possessing a strong stimulating effect on nerve growth factor (NGF) synthesis as well as immunostimulating, cytotoxic and antibacterial activity. The aim of the thesis was to isolate low-molecular weight metabolites from a liquid culture of H. erinaceum, to deve...

Mycelium of the white-rot fungus (Hericium erinaceum (Bull.: Fr. Pers.) produces polysaccharides showing anticancer and immunostimulating activity. In our previous works, we have shown that organic selenitetriglycerides (Selol) contribute to the increase of biosynthesis of exopolysaccharides (EPS) having antioxidative properties and containing larg...

This paper describes the validation of an HPLC method for quantitative determination of erinacine A as well as optimization of the culture medium composition for its effective production in submerged culture of the edible and medicinal mushroom Hericium erinaceum. The effects of different medium components were examined by the one-factor-at-a-time...

The publication gives a review of therapeutic activities of 8 edible mushrooms. These are members of the genus: Lentinus, Grifolia, Pleurotus, Flammulina, Agaricus, Hirneola (Auricularia) and Ganoderma. Particularly discussed are the immunomodulatory and antineoplastic acitivities. These are related mainly to ß-1,3-glucans, which act on the immune...

The publication gives a review of therapeutic activities of 8 edible mushrooms. These are members of the genus: Lentinus, Grifolia, Pleurotus, Flammulina, Agaricus, Hirneola (Auricularia) and Ganoderma. Particularly discussed are the immunomodulatory and antineoplastic acitivities. These are related mainly to ß-1,3-glucans, which act on the immune...

The aim of this study was to optimize the culture medium used for the mycelial growth and production of intracellular polysaccharides (IPS) and exopolysaccharides (EPS) in a submerged culture of Hericium erinaceum. Of the various factors examined, including carbon and nitrogen sources, vitamins, mineral elements, and initial pH, those that proved t...

The effects of inorganic selenium (sodium selenite) and Selol containing selenitetriglycerides synthesized from sunflower oil on mycelial growth and selenium-containing extracellular (EPS) and intracellular (IPS) polysaccharides production were examined in shake flask cultures of Hericium erinaceum.Unlike sodium selenite which inhibited mycelial gr...

Ergosterol peroxide, the steroidal derivative with cytotoxic activity, has been isolated for the first time from the mycelium of edible and medicinal mushroom Hericiumerinaceum (lion’s mane mushroom) together with erinacine A. The new densitometric method was applied for the quantitative determination of ergosterol peroxide in n-hexane extracts of...

Preparations derived from Lentinula edodes (Berk.) Pegl. are widely used as dietary supplements, they contain compounds with immune system enhancing and chemopreventive properties. These preparations contain a blend of minerals and vitamins, including vitamins A, D, B1, B2, C, and niacin, but only scant vitamin B12. Our goal was to optimize the gro...

In recent years mushroom polysaccharides have been extensively investigated according to their unique biological activity. Among them polysaccharides from Hericium erinaceum (Hydnaceae) are well known as anti-tumor and cholesterol reducing agents as well as growth and differentiation of adrenal nerve cells stimulators. In Poland, H. erinaceum is re...

This paper describes an unusual type of diterpenoids, called cyathins, and presents their structure, biosynthesis and biological activities. These secondary metabolites have been isolated during the last 35 years from mycelia, culture broths and fruiting bodies of several species of mushrooms such as Cyathus helenae, C. striatus, Hericium erinaceum...