Witsanu Srila

Witsanu Srila
Rajamangala University of Technology · Biology

Doctor of Philosophy
Cell engineering

About

16
Publications
5,659
Reads
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200
Citations
Additional affiliations
May 2024 - present
Rajamangala University of Technology
Position
  • Lecturer
Education
July 2015 - September 2020
Suranaree University of Technology
Field of study
  • Biotechnology

Publications

Publications (16)
Article
Full-text available
Background/Objectives: Halitosis is primarily caused by the activity of oral microorganisms. In this study, we employed metagenomic sequencing and metabolomic approaches to investigate the differences in salivary microbiota and metabolite profiles between individuals with halitosis and periodontitis and healthy controls. Additionally, we expanded t...
Article
Myogenic differentiation ability made porcine muscle satellite cells (MSCs) promising candidates for cultured meat production. While in vitro, porcine primary cells lose differentiation capacity, have short lifespans, and change phenotype. For immortal porcine MSCs, human telomerase reverse transcriptase (hTERT) gene was overexpressed in parental c...
Chapter
A simple and rapid method for reproducible transient expression experiments to quickly identify the best conditions before the development of a stable CHO cell line is described, using adalimumab as a model antibody. Firstly, the signal peptide (SP) is optimized using different in silico programs, of which those having a D score of more than 0.85 w...
Article
Full-text available
The glutamine synthetase (GS)-based Chinese hamster ovary (CHO) selection system is an attractive approach to efficiently identify suitable clones in the cell line generation process for biologics manufacture, for which GS-knockout (GS-KO) CHO cell lines are commonly used. Since genome analysis indicated that there are two GS genes in CHO cells, de...
Article
Full-text available
The article “Production and applications of fluorobody from redox‑engineered Escherichia coli” was originally published Online First without open access. After publication in volume 107, issue 5-6, page 1959–1970, the author decided to opt for Open Choice and to make the article an open access publication.
Article
Full-text available
Efficient selection and production of antibody fragments in microbial systems remain to be a challenging process. To optimize microbial production of single-chain variable fragments (scFvs), we have chosen five model targets, 1) a hapten, Zearalenone (ZEN) mycotoxin, along with infectious agents 2) rabies virus, 3) Propionibacterium acnes, 4) Pseud...
Article
Full-text available
Ochratoxin A (OTA) is a mycotoxin commonly found in agricultural products and can accumulate in the blood and tissues after that consuming contaminated food. Recombinant single-chain antibody fragments (scFv) against OTA were selected from phage display libraries. After of one round of biopanning against BSA-conjugated OTA (OTA-BSA), 52 and 6 phage...
Article
Full-text available
A simple and reliable method for the detection of specific nitrogen-fixing bacteria in both free-living and bacteroid forms is essential for the development and application of biofertilizer. Traditionally, a polyclonal antibody generated from an immunized rabbit was used for detection. However, the disadvantages of using a polyclonal antibody inclu...
Article
Full-text available
Abstract Mannans of different structure and composition are renewable bioresources that can be widely found as components of lignocellulosic biomass in softwood and agricultural wastes, as non-starch reserve polysaccharides in endosperms and vacuoles of a wide variety of plants, as well as a major component of yeast cell walls. Enzymatic hydrolysis...
Article
The effects of water treated with an electromagnetic field (EMF) were investigated on two biological systems, humans and plants. Purified de-ionised water was treated by (1) boiling, (2) exposure to microwave radiation, and (3) low frequency electromagnetic oscillation molecular resonance effect technology (MRET), before being used to prepare media...
Article
Full-text available
FLAG, a short hydrophilic peptide consisting of eight amino acids (DYKDDDDK), has been widely used as a fusion tag for the purification and detection of a wide variety of recombinant proteins. One of the monoclonal antibodies against this peptide, anti-FLAG M2, recognises a FLAG peptide sequence at the N terminus, Met-N terminus, C terminus, or int...
Article
Full-text available
β-Galactosidase enzyme catalyzes not only the hydrolysis reaction but also the transgalactosylation reaction of β-D-galactopyranosidic linkage. Hence, β-galactosidase is among the most common industrial enzymes due to its various applications in dairy and food industries. More recently, thermophilic bacteria have been of interests for the commercia...
Article
Full-text available
Galactosidase is a commercial enzyme that are normally used for solving lactose consumption and utilisation. In this study, thermophilic microorganisms producing β-galactosidase were isolated from Ta Pai hot spring, Maehongson, Thailand. For screening β-galactosidase activity, all strains were plated on lactose agar containing 5-bromo-4-chloro-3-id...

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