Winiati Pudji Rahayu

• Faculty Member at IPB University

Food product testing laboratories play a prominent role in ensuring safety and quality of products manufactured by food industries; therefore, they shall comply with standards of ISO/IEC 17025:2017. Although a proficiency test for microbiological parameter is required to evaluate testing laboratories' performance in considering food product quality...

Red palm oil (RPO), processed without bleaching, has a higher total carotenoid content compared to commercial frying oil (CFO), at 500-700 ppm. The use of RPO in household cooking is not widespread due to its different characteristics compared to the commonly used cooking oil. This research aimed to determine the physicochemical characteristics (co...

Contamination of Aspergillus flavus (A. flavus) and aflatoxin in nutmeg remains problematic due to the poor postharvest handling practices and the climate, which is favorable for fungal growth in Indonesia. Using the natural antifungal agents can be an alternative measure to control the aflatoxin-producing fungal growth. This current study aimed to...

Reuterin is a natural antifungal agent derived from certain strains of Limosilactobacillus reuteri. Our previous study revealed that 6 mM reuterin inhibited completely the conidial germination of aflatoxigenic Aspergillus flavus. This study investigated the potential molecular mechanism of reuterin in inhibiting A. flavus conidial germination, whic...

The application of food safety culture (FSC) which is influenced by human behavior with the development of industrial technology (IT) 4.0 with less human resources provides a challenge to understand the relationship between them. The research objective was to provide quantitative data and suggestions for improving the implementation of the FSC dime...

Production and export of tropical fruits in Indonesia have an increasing trend every year. The important factor in the production and export of fruits is food safety. One of the contaminants that may be found in fruits is mycotoxin, especially patulin. Patulin mainly found in fruits such as apple, orange, grape, and pear. This study aimed to detect...

Reuterin is a secondary metabolite of the lactic acid bacteria Limosilactobacillus reuteri having broad antimicrobial activity. This study aimed to determine the antifungal activity of reuterin against aflatoxigenic Aspergillus flavus (A. flavus). The aflatoxigenic A. flavus BIO 33212 isolated from Indonesian nutmeg was used in this study. The mini...

Identification and sequencing of Salmonella require a specific primer as well as a virulence marker. Invasion gene (invA) is a gene found in all types of Salmonella, specific to Salmonella, and has a low mutation rate so that it can be used as a target gene for Salmonella identification. This study aimed to design a primer of invA gene of Salmonell...

Aflatoxin M1 (AFM1) is a mycotoxin that often contaminates milk. Like other mycotoxins, it is thermostable and potentially carcinogenic. The present work was carried out to evaluate the ability of microorganisms isolated from Indonesian kefir grains to reduce AFM1 in contaminated phosphate buffer saline (PBS). Fourteen isolates of lactic acid bacte...

Mycotoxin is one of the food safety problems that raise concern because of its negative health impact and significant economic losses. Patulin is the most common mycotoxin found in fruits and its products like apple, orange, grape, pear, etc. There are 167 species reported to produce patulin; Penicillium expansum is the major patulin producer among...

Differences between auditor perceptions often occur in the audit process of the management system, even auditors from the Food Safety Management System Certification Body (FSMS CB). The auditor as an assessor is able to influence strongly to the audit report. A scoring system is a quantitative approach in auditing to eliminate subjectivity and impa...

Salmonella spp. is Gram negative-pathogenic bacteria that usually found as a contaminant in chicken carcasses. This study was aimed to increase the sensitivity of PCR enrichment step and apply the enrichment-PCR combination to detect Salmonella in chicken carcasses. In this study were used Salmonella enterica serovar Hadar, Salmonella enterica sero...

Food safety is an important aspect of consumption behavior, especially for employees in the office. Lack of food safety knowledge and awareness can endanger the health and reduce the productivity of employee. This study aims to describe the level of food safety knowledge and attitudes of employees at food stalls as well as their preferences for lun...

Homemade foods have been reported as an important contributor to some foodborne outbreaks. This study determined the prevalence and number of Salmonella spp. on selected pathways of fried chicken preparation and cooking at the household level and investigated their antimicrobial resistance. Salmonella serovar was confirmed by polymerase chain react...

Catering industry is a food business which produces ready-to-eat food or food that will not be cooked or reheated before serving. Therefore, the safety of food produced by catering industry becomes very important. During period of 2015–2017, the food produced by catering industry was the third largest cause of food borne outbreak in Indonesia. This...

Real-time polymerase chain reaction (qPCR) technique is a suitable method for identifying animal species in processed meat because of its ability to amplify a few fragments of DNA. A specific fragment of pork (mitochondrial cytochrome b gene (cytb)) was used as a DNA ladder. This study aimed to evaluate the use of a cyt-b gene generated primer for...

Food for school children may become unsafe if its raw materials and handling processes are not well controlled. This study aimed to determine the level of compliance of the vendors with good processing practices. The method used was the development of vendor compliance criteria, observation of vendors’ behavior, and exploration of the source of the...

Pathogenic Escherichia coli (E. coli) has been implicated in a wide range of disease causing infections. It is essential to generate a method for detecting and differentiating each pathotype of E. coli which is more quickly and efficiently by using less reagent. This study aimed to evaluate a SYBR Green multiplex real-time PCR method for detecting...

Background: Besides their mutagenic, carcinogenic, and teratogenic effects, Aflatoxins (AFs) also act as the main contributor to hepatocellular carcinoma (HCC) which is known as the most common primary liver cancer worldwide. The main aim of this study was risk estimation of HCC due to exposure to aflatoxins in maize from Yogyakarta, Indonesia. Met...

This study was aimed to determine the prevalence, the level and the main contributing factors to contamination of Salmonella spp. in four selected chicken-based side dishes prepared for the school canteens. One hundred and seven samples were collected from four different food processing chains, i.e. fried chicken with precooking, fried chicken with...

Salmonella is a pathogenic bacterium that can cause serious harm to humans. Chicken carcasses have been reported contaminated by Salmonella, especially S. Typhimurium and S. Enteritidis. These two serovars are very difficult to be confirmed and distinguished using biochemical analysis, therefore a rapid method for detection and differentiation of b...

The objective of this study was to investigate the antifungal activity of kebar grass (Biophytum petersianum Klotzsch) extract (KGE) on the mycelial growth, conidiation and morphological structure of two types of aflatoxigenic fungus, which are Aspergillus flavus BCCF 0219 and A. flavus BIO 2236. They were isolated in the three types of model media...

Consumption pattern of iced beverages of the consumer in Bogor and their awareness of food safety Background: Iced beverage will be potentially contaminated by microbes if it is prepared and handled by a lack of sanitary and hygiene. The level of risk depends on exposure affected by the prevalence and concentration of microorganisms, also the cons...

Pangan dapat terkontaminasi oleh cemaran kimia karena penanganan dan pengolahan pangan yang tidak sesuai. Tujuan dari penelitian ini adalah 1) mengidentifikasi mayoritas penyebab penolakan produk perikanan Indonesia, 2) menelaah standar cemaran kimia pada produk perikanan, khususnya logam berat yang ada di Indonesia, Codex Alimentariurs Commision (...

The consumption of fresh vegetables at the stalls needs serious attention. This research aimed to estimate the exposure probability due to fresh vegetables consumption of street food consumers, to measure sanitation level of street stalls that serving fresh vegetables, and to recommend a mentoring program for the stalls at four locations in Bogor C...

Aspergillus flavus is one of the important fungus which can cause nutmeg deterioration during storage. The study aimed to investigate the effect of gamma irradiation 5 and 10 kGy on conidial germination and successive growth Aspergillus flavus strains isolated from nutmeg kernels up to 20 generations. Thirteen strains of the A. flavus were sub-cult...

Mycotoxin is widely known as one cause of foodborne disease, produced by toxigenic fungi. Any country should be aware about this high risk potency by knowing the mycotoxin, affected commodities, fungal sources, and toxicity effect to human or animal. Controlling mycotoxin could be done by physic, chemical, and biological methods. The microbial char...

A study on the effectiveness of gamma irradiation at doses of 5 and 10 kGy on fungal population, Aspergillus flavus strains, and aflatoxin B1 contamination on stored nutmeg kernels was conducted. The kernels were collected from seeds in a period of one week from the ground at North Sulawesi Province, Indonesia. Dried shelled kernels with ± 10 % moi...

The aim of this investigation was to determine the effect of various water activities (aw) on fungal population in nutmeg kernels during storage. The seed nutmegs were obtained from ripe fruits one week after they fell on the ground in North Minahasa Regency, North Sulawesi Province, Indonesia. The kernels (moisture content ± 10%) were stored 0, 15...

Fish-based snacks, such as siomay (steamed fish dumpling), otak-otak (fish-dough sticks), and pempek (fried fish-dough native to Palembang, Indonesia), are being vend around the schools in Indonesia. This study was aimed to identify critical points of the snacks' preparation line, therefore the possible hazards from physical,microbiological, and ch...

Wild mango (Spondias pinnata) leaves is a very popular herb in Southeast Sulawesi, has been used as herbal medicine at Southern and Southeastern Asia. The purpose of this study was to determine the antibacterial activity, cytotoxicity and phytochemical contents of wild mango (Spondias pinnata) leaves extracts. The extraction was performed using a c...

Unbalanced dietary consumption, lack of dietary fiber intake and low physical activity could lead to excessive nutrients resulting in obesity. This research aims to create high-fiber snack food in the form of snack bar made from pulp of tofu flour (okara), purple sweet potato flour, and cornstarch. The experimental design was a complete factorial r...

Abstrak Minuman es seperti es kelapa, es buah, es campur, jus buah, es teh dan es dengan cincau banyak ditemukan di kota Bogor dan harus terjamin keamanannya. Penelitian ini bertujuan untuk mengetahui karakteristik dan kondisi pedagang minuman es, serta tingkat kepatuhannya terhadap pelaksanaan good manufacturing practices/cara produksi pangan yan...

The aims of this work were to investigate the effects of gamma irradiation on population, viability and aflatoxin B1 production of Aspergillus flavus strains isolated from nutmeg kernels. Thirteen strains of A. flavus were isolated and cultured in potato dextrose agar. Conidia were harvested, air-dried, and irradiated 0, 5, or 10 kGy with gamma ray...

One strategy that can be taken to develop the economic potential of fruits is the logistical support that takes into account the effectiveness and efficiency commercially and pay attention to quality and food safety requirements. Practically, logistics support can be done with the application of good logistics practices (GLP). Packaging, storage, a...

The utilization of Indonesia’s fisheries potential should be supported by the development of fisheries industry, more specifically by the improvement on fisheries supply chain management. It is needed to preserve the quality and safety of fisheries product from sea to consumers’ table and the sustainable fisheries supply. The fisheries product is p...

Introduction: Ice can be contaminated by pathogenic bacteria. This study aimed to identify critical points in iced beverage production and distribution lines to examine the presence of pathogenic bacteria in a beverage and its processing environment, as well as when water and ice used as main ingredients. Methodology: The critical points were deter...

p>Kapang toksigenik Aspergillus ochraceus penghasil mikotoksin dapat menimbulkan masalah kesehatan bila mengkontaminasi bahan pangan seperti jagung dan kedelai. Pertumbuhan kapang A. ochraceus dipengaruhi oleh perubahan iklim, seperti perubahan suhu dan kelembaban. Penelitian ini bertujuan untuk mengevaluasi pengaruh perubahan suhu dan kelembaban t...

Estimates for the burden (number of cases) and cost of diarrhoeal foodborne illness (FBI) per year in Indonesia were calculated. Distinct data sources and models (derived from the World Health Organi-sation, and Indonesian sources) were used to cross-validate results from each analysis. For all cases, estimates of the burdens and costs of the foodb...

Green mussel (Perna viridis) and cockle shell (Anadara granosa) are one of many sources of animal protein which is many cultivated in Indonesia because their price is relatively affordable. This study was conducted to identify the presence of Listeria monocytogenes in 27 samples of green mussels and 3 samples of cockle shells using real-time Polyme...

The purpose of the study is to assess the effect of the use of mono probiotic culture and mixed cultures of Lactobacillus plantarum and Lactobacillus rhamnosus on the physicochemical properties and its viability during storage. The soft cheeses were made through three treatments: (1) the use of Lactobacilllus rhamnosus culture, (2) the use of Lacto...

The utilization of Indonesia’s fisheries potential should be supported by the development of fisheries industry, more specifically by the improvement on fisheries supply chain management. It is needed to preserve the quality and safety of fisheries product from sea to consumers’ table and the sustainable fisheries supply. The fisheries product is p...

The utilization of Indonesia’s fisheries potential should be supported by the development of fisheries industry, more specifically by the improvement on fisheries supply chain management. It is needed to preserve the quality and safety of fisheries product from sea to consumers’ table and the sustainable fisheries supply. The fisheries product is p...

Aims: Listeria monocytogenes is an important foodborne pathogen that can cause serious human listeriosis. The aim of this study was to optimize real-time PCR method for L. monocytogenes detection in indigenous Indonesian snack (pempek). Methodology and results: DNA isolation of L. monocytogenes was conducted by comparing three extraction methods: p...

Deterioriation of palm oil fortified with vitamin A and provitamin A could be caused by the presence of oxygen and light exposure. The purpose of this study was to determine the effect of the initial peroxide value (PV) in palm oil (1.99, 3.98, and 9.95 meq O2/kg oil) and light intensity (15000, 10000, and 5000 lux) on the rate of oxidation and the...

L. monocytogenes contamination in processed food, especially fish-based snack, may results from contaminated raw materials, underprocessed or recontamination. The aims of this study were to identify the presence of Listeria spp. especially L. monocytogenes by PCR method and biochemical methods, as well as calculate the prevalence of Listeria spp. i...

Food poisoning outbreaks frequently occur in many countries resulting in economic loss. A formula for calculation of economic loss due to food poisoning outbreaks in Indonesia was developed. Estimation of the loss was developed based on experiences in several countries. Related data were also collected from local health facilities, food industry of...

The purpose of the study is to assess the effect of the use of mono probiotic culture and mixed cultures of Lactobacillus plantarum and Lactobacillus rhamnosus on the physicochemical properties and its viability during storage. The soft cheeses were made through three treatments: (1) the use of Lactobacilllus rhamnosus culture, (2) the use of Lacto...

Food for school children holds an important role in the fulfillment nutritional intake of children at the school age, therefore it has to be safe for consumption. The research that had been conducted in Bogor to thirty five samples from twenty two food sellers in eight elementary schools in two different time periods. The analysis results showed th...

Arrowroot starch can be modified to produce resistant starch (RS), which have been observed to show physiological functions similar to dietary fiber. Acid hydrolysis and autoclaving-cooling cycling treatments can increase RS content. Arrowroot starch modification hydrolyzed by 1.1 and 2.2 N HCl for 0, 2, 4, and 6 hours, then continued with 3 cycles...

was the predominant species in producing fumonisin on agricultural products. Fumonisisn B1 (FB1) is the most abundant fumonisin in nature and the most toxic than other fumonisin. The main factors affecting the growth of and production of fumonisin are temperature and humidity. This research aimed to assess the effect of changes in temperature and h...

The aims of this study was to determine the characteristics, composition and antimicrobial activity of essential oils of local Indonesian red ginger and red galangal against four pathogenic and food spoilage bacteria, which were B.cereus ATCC 10876, E. coli ATCC 25922, S. typhimuriumATCC 14028, and P. aeruginosa ATCC 27853. Analysis of physicochemi...

Aflatoxin B1 was a secondary metabolite produced by Aspergillus flavus having negative effect on human health because of its carcinogenic. Many efforts have been done to investigate the antifungal and antiaflatoxin agent derived plant. The objective of this research was to study the activity of antifungal from kebar grass leaf extract on mycelial grow...

One strategy that can be taken to develop the economic potential of fruits is the logistical support that takes into account the effectiveness and efficiency commercially and pay attention to quality and food safety requirements. Practically, logistics support can be done with the application of good logistics practices (GLP). Packaging, storage, a...

ABSTRAK Aflatoksin B 1 merupakan metabolit sekunder yang dihasilkan oleh Aspergillus flavus yang berbahaya bagi kesehatan karena bersifat karsinogenik. Berbagai upaya telah dilakukan untuk mencari bahan antikapang dan antiaflatoksin yang berasal dari bahan alami seperti tumbuhan. Tujuan penelitian ini adalah mempelajari aktivitas ekstrak daun rumpu...

Aspergillus flavus (A. flavus) producing aflatoxin frequently contaminates crops such as soybeans. The growth of this mold on soybeans and other foodstuffs is affected by temperature and relative humidity (RH). The aim of this study was to measure the growth of A. flavus BIO 2237 and aflatoxin production at different temperatures and RH. Aspergillu...

Toxigenic fungi, Aspergillus ochraceus producing ochratoxin A can cause serious health problem if the fungi contaminate food product such as maize and soybean. A. ochraceus growth is affected by climate change including the change of temperature and relative humidity. This study was performed to evaluate the effect of temperature and relative humid...

Indonesia has more than 230 million people of diverse ethnic groupings whose dietary habits are quite variable. Given this condition, some efforts have been carried out to prepare and develop a national total diet study (TDS) in Indonesia. Since 2002, the National Agency for Drug and Food Control has initiated a number of pilot studies are summariz...

The objective of this research was to utilize the local probiotic lactic acid bacteria, isolated from beef in some traditional markets in Bogor (L. plantarum 2C12 and L. fermentum 2B4), in synbiotic yogurt making and analyzing its antidiarrheal and antioxidative capability in rat models. The rats were treated with synbiotic yogurt and enteropathoge...

Pemberdayaan Usaha Mikro, Kecil dan Menengah (UMKM), termasuk UMKM di bidang pangan, merupakan upaya strategis karena UMKM merupakan barometer perekonomian nasional yang mampu menyumbang kurang lebih 55% pendapatan per kapita. Secara khusus, selain merupakan unit-unit kegiatan ekonomi, UMKM di bidang pangan juga menghasilkan produk pangan yang memi...

The use of goat milk is limited in Indonesia due to lack of good milking practices resulted in disliked goaty smell. One of the method to eliminate this off flavor is by processing the goat milk into soft cheese. The aim of this research was to study the stability of viable starter lactic acid bacteria cultures ( Lactobacillus acidophilus FNCC-0051...

Tempeh is a traditional Indonesian food obtained from fermentation of soybeans using Rhizopus oligosporus. Tempe is one of the main protein sources in Indonesian diet. Unfortunately, fresh tempeh has short shelf life. Even though thermal process has been applied to extend its shelf life, study on the effect of sterilization dosage (F0), that can be...