William C Wilson

William C Wilson
United States Department of Agriculture | USDA · Agricultural Research Service (ARS)

PhD

About

270
Publications
20,616
Reads
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4,115
Citations
Additional affiliations
June 1986 - present
United States Department of Agriculture
Position
  • Research Microbiologist
June 1984 - June 1986
University of Nebraska Medical Center
Position
  • PostDoc Position
May 1979 - June 1984
University of Illinois, Urbana-Champaign
Position
  • PhD Student

Publications

Publications (270)
Article
Full-text available
PCR amplification technology for the detection of epizootic hemorrhagic disease virus (EHDV) ribonucleic acid in cell culture and clinical specimens was developed. With oligoribonucleotide primers selected from ge-nome segment 10 of EHDV serotype 1 (EHDV-1), which codes for two nonstructural proteins (NS3 and NS3a), the PCR-based assay resulted in...
Chapter
In 2016, the International Committee on Taxonomy of Viruses elevated the family Bunyaviridae to the order Bunyavirales to incorporate an increasing number of identified related viruses with new family classifications. The bunyaviruses are generally susceptible to drying, heat, acids, bleaches, deter gents, and most common disinfectants. The genus O...
Chapter
Viruses in the Reoviridae family infect a wide range of species, including mammals, fish and shellfish, insects, plants, protists, and fungi. Mammalian orthoreoviruses have been isolated from the respiratory and/or gastrointestinal tract of many animal species, including nonhuman primates, rodents, horses, cattle, sheep, swine, cats, and dogs. Orth...
Article
Full-text available
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for a global pandemic that has had significant impacts on human health and economies worldwide. SARS-CoV-2 is highly transmissible and the cause of coronavirus disease 2019 in humans. A wide range of animal species have also been shown to be susceptible to SARS-CoV-2 by exp...
Article
Full-text available
Rift Valley fever virus (RVFV) is a mosquito-borne, zoonotic phlebovirus-causing disease in domestic ruminants and humans in Africa, the Arabian Peninsula and some Indian Ocean islands. Outbreaks, characterized by abortion storms and a high morbidity rate in newborn animals, occur after heavy and prolonged rainfalls favouring the breeding of mosqui...
Article
Full-text available
Rift Valley fever virus (RVFV) is an arbovirus that was first reported in the Rift Valley of Kenya which causes significant disease in humans and livestock. RVFV is a tri-segmented, negative-sense RNA virus consisting of a L, M, and S segments with the M segment encoding the glycoproteins Gn and Gc. Host factors that interact with Gn are largely un...
Article
This communication summarizes the presentations given at the 1st international conference of the World Society for Virology (WSV) held virtually during 16–18 June 2021, under the theme of tackling global viral epidemics. The purpose of this biennial meeting is to foster international collaborations and address important viral epidemics in different...
Article
Full-text available
SARS-CoV-2 was first reported circulating in human populations in December 2019 and has since become a global pandemic. Recent history involving SARS-like coronavirus outbreaks have demonstrated the significant role of intermediate hosts in viral maintenance and transmission. Evidence of SARS-CoV-2 natural infection and experimental infections of a...
Preprint
Full-text available
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for a global pandemic that has had significant impacts on human health and economies worldwide. SARS-CoV-2 is highly transmissible and the cause of coronavirus disease 2019 (COVID-19) in humans. A wide range of animal species have also been shown to be susceptible to SARS-C...
Article
Full-text available
Epizootic hemorrhagic disease (EHD) is an insect-transmitted viral disease of wild and domestic ruminants. It was first described following a 1955 epizootic in North American white-tailed deer (Odocoileus virginianus), a species which is highly susceptible to the causative agent of EHD, epizootic hemorrhagic disease virus (EHDV). EHDV has been dete...
Article
Full-text available
This paper reports on validation of diagnostic performance of an indirect enzyme-linked immunosorbent assay (I-ELISA) based on a recombinant nucleocapsid protein (rNP) of the Rift Valley fever virus (RVFV) for the detection of the IgG antibody in sheep (n = 3367), goat (n = 2632), and cattle (n = 3819) sera. Validation data sets were dichotomized...
Preprint
Full-text available
SARS-CoV-2, a novel Betacoronavirus, was first reported circulating in human populations in December 2019 and has since become a global pandemic. Recent history involving SARS-like coronavirus outbreaks (SARS-CoV and MERS-CoV) have demonstrated the significant role of intermediate and reservoir hosts in viral maintenance and transmission cycles. Ev...
Article
Full-text available
Rift Valley fever virus (RVFV) is a mosquito-borne zoonotic pathogen that causes periodic outbreaks of abortion in ruminant species and hemorrhagic disease in humans in sub-Saharan Africa. These outbreaks have a significant impact on veterinary and public health. Its introduction to the Arabian Peninsula in 2003 raised concerns of further spread of...
Article
Full-text available
Bluetongue virus (BTV) is a segmented RNA virus transmitted by Culicoides midges. Climatic factors, animal movement, vector species, and viral mutation and reassortment may all play a role in the occurrence of BTV outbreaks among susceptible ruminants. We used two enzootic strains of BTV (BTV-2 and BTV-10) to explore the potential for Culicoides so...
Article
Full-text available
Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a recently emerged coronavirus that is the causative agent of the coronavirus disease 2019 (COVID-19) pandemic. COVID-19 in humans is characterized by a wide range of symptoms that range from asymptomatic to mild or severe illness including death. SARS-CoV-2 is highly contag...
Article
Full-text available
The recent impact of Ebola virus disease (EVD) on public health in Africa clearly demonstrates the need for a safe and efficacious vaccine to control outbreaks and mitigate its threat to global health. ERVEBO® is an effective recombinant Vesicular Stomatitis Virus (VSV)-vectored Ebola virus vaccine (VSV-EBOV) that was approved by the FDA and EMA in...
Article
Full-text available
SARS-CoV-2 is the causative agent of COVID-19 and responsible for the current global pandemic. We and others have previously demonstrated that cats are susceptible to SARS-CoV-2 infection and can efficiently transmit the virus to naïve cats. Here, we address whether cats previously exposed to SARS-CoV-2 can be re-infected with SARS-CoV-2. In two in...
Article
Full-text available
SARS-CoV-2 is a recently emerged, highly contagious virus and the cause of the current COVID-19 pandemic. It is a zoonotic virus, although its animal origin is not clear yet. Person-to-person transmission occurs by inhalation of infected droplets and aerosols, or by direct contact with contaminated fomites. Arthropods transmit numerous viral, paras...
Preprint
Full-text available
SARS-CoV-2 is the causative agent of COVID-19 and responsible for the current global pandemic. We and others have previously demonstrated that cats are susceptible to SARS-CoV-2 infection and can efficiently transmit the virus to naive cats. Here, we address whether cats previously exposed to SARS-CoV-2 can be re-infected with SARS-CoV-2. In two in...
Article
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the cause of Coronavirus Disease 2019 (COVID-19) and responsible for the current pandemic. Recent SARS-CoV-2 susceptibility studies in cats show that the virus can replicate in these companion animals and transmit to other cats. Here, we present an in-depth study of SARS-CoV-2 infectio...
Preprint
Full-text available
SARS-CoV-2 is a recently emerged, highly contagious virus and the cause of the current pandemic. It is a zoonotic virus, although its animal origin is not clear yet. Person-to-person transmission occurs by inhalation of infected droplets and aerosols, or by direct contact with contaminated fomites. Arthropods transmit numerous viral, parasitic, and...
Preprint
Full-text available
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the cause of Coronavirus Disease 2019 (COVID-19) and responsible for the current pandemic. Recent SARS-CoV-2 susceptibility and transmission studies in cats show that the virus can replicate in these companion animals and transmit to other cats. Here, we present an in-depth study of SA...
Article
Full-text available
African swine fever virus (ASFV) is the sole member of the family Asfarviridae, and the only known DNA arbovirus. Since its identification in Kenya in 1921, ASFV has remained endemic in Africa, maintained in a sylvatic cycle between Ornithodoros soft ticks and warthogs (Phacochoerus africanus) which do not develop clinical disease with ASFV infecti...
Article
Full-text available
Since the discovery of Rift Valley Fever virus (RVFV) in Kenya in 1930, the virus has become widespread throughout most of Africa and is characterized by sporadic outbreaks. A mosquito-borne pathogen, RVFV is poised to move beyond the African continent and the Middle East and emerge in Europe and Asia. There is a risk that RVFV could also appear in...
Article
Rift Valley fever virus (RVFV) causes severe disease in domestic ungulates (cattle, goats, and sheep) and a febrile illness in humans (with ∼1% case fatality rate). This virus has been spreading geographically, and there is concern of it spreading to Europe or the Americas. Environmental temperature can significantly affect the ability of mosquitoe...
Article
Full-text available
Epizootic hemorrhagic disease virus (EHDV) is an arthropod-transmitted RNA virus and the causative agent of epizootic hemorrhagic disease (EHD) in wild and domestic ruminants. In North America, white-tailed deer (WTD) experience the highest EHD-related morbidity and mortality, although clinical disease is reported in cattle during severe epizootics...
Article
Rift Valley fever virus (RVFV) is an arthropod‐borne, zoonotic disease endemic to sub‐Saharan Africa and the Arabian Peninsula. Outbreaks of Rift Valley fever have had up to 100% mortality rates in fetal and neonatal sheep. Upon infection of ruminant and human hosts alike, RVFV infection causes an at times severe hepatitis and pathology in many oth...
Article
Full-text available
First appearing in 2011 in Northern Europe, Schmallenberg virus (SBV), an Orthobunyavirus of the Simbu serogroup, is associated with clinical disease mainly in ruminants such as cattle, sheep and goats. The clinical signs are characterized by abortion and congenital deformities in newborns. The virus is transmitted by Culicoides midges of the Obsol...
Article
Full-text available
The Schmallenberg virus (SBV) is an orthobunyavirus that causes abortions, stillbirths, and congenital defects in pregnant sheep and cattle. Inactivated or live attenuated vaccines have been developed in endemic countries, but there is still interest in the development of SBV vaccines that would allow Differentiating Infected from Vaccinated Animal...
Article
The increasing risk of Rift Valley fever virus (RVFV) as a global veterinary and public health threat demands the development of safe and accurate diagnostic tests. The aim of this study was to assess the suitability of a baculovirus expression system to produce recombinant RVFV nucleoprotein (N) for use as sero-diagnostic antigen in an indirect en...
Article
First described in 1955 in New Jersey, epizootic hemorrhagic disease (EHD) causes a severe clinical disease in wild and domestic ruminants worldwide. EHD outbreaks occur in deer populations each year from summer to late autumn. The etiological agent is EHD virus (EHDV) which is a double‐stranded segmented icosahedral RNA virus. EHDV utilizes point...
Article
The full-genome sequence of bluetongue virus serotype 3 (BTV-3) USA2016/LA CC16-564, isolated from a white-tailed deer in East Feliciana Parish, Louisiana, is reported here. Nine genomic segments of this virus have 99% identity with a 2013 BTV-3 isolate from Florida, while segment 10 has 97% identity with 2003 BTV-5 and 2006 BTV-2 isolates from Flo...
Article
Full-text available
Epizootic hemorrhagic disease viruses (EHDVs) are arboviral pathogens of white-tailed deer and other wild and domestic ruminants in North America. Transmitted by various species of Culicoides, EHDVs circulate wherever competent vectors and susceptible ruminant host populations co-exist. The impact of variation in the level and duration of EHDV vire...
Article
Rift Valley fever virus (RVFV) is an important mosquito-borne pathogen with devastating impacts on agriculture and public health. With outbreaks being reported beyond the continent of Africa to the Middle East, there is great concern that RVFV will continue to spread to non-endemic areas such as the Americas and Europe. There is a need for safe and...
Article
Full-text available
Following a summer of severe drought and abnormally high temperatures, a major outbreak of EHDV occurred during 2012 in the USA. Although EHDV-1, -2 and -6 were isolated, EHDV-2 was the predominant virus serotype detected during the outbreak. In addition to large losses of white-tailed deer, the Midwest and northern Plains saw a significant amount...
Article
Rift Valley fever virus (RVFV) is the causative agent of Rift Valley fever (RVF) that affects both livestock and humans. There are neither fully licensed RVF vaccines available for human or animal use, nor effective antiviral drugs approved for human use in the U.S. To identify antiviral compounds effective for RVF, we developed and employed a cell...
Article
Full-text available
Rift Valley fever phlebovirus (RVFV) is a mosquito-transmitted pathogen endemic to sub-Saharan Africa and the Arabian Peninsula. RVFV is a threat to both animal and human health and has costly economic consequences mainly related to livestock production and trade. Competent hosts and vectors for RVFV are widespread, existing outside of endemic coun...
Article
Bluetongue (BT) and epizootic hemorrhagic disease (EHD) are globally re-emerging diseases of domestic and wild ruminants, respectively caused by BT virus (BTV) and EHD virus. Both viruses are transmitted by hematophagous midges; however, newly recognized BTV serotypes may be transmitted horizontally without requirement for any biological vector. Th...
Article
Full-text available
Rift Valley fever virus, a zoonotic arbovirus, poses major health threats to livestock and humans if introduced into the United States. White-tailed deer, which are abundant throughout the country, might be sentinel animals for arboviruses. We determined the susceptibility of these deer to this virus and provide evidence for a potentially major epi...
Article
Full-text available
Schmallenberg virus (SBV) is an orthobunyavirus in the Simbu serogroup that emerged in Germany in late 2011 and was mostly associated with a mild transient disease of sheep and cattle. SBV is transmitted by biting midges (Culicoides species) and causes abortions, stillbirths, and congenital defects in naïve pregnant ruminants. Two separate studies...
Article
Full-text available
A robust immunoassay based on surface enhanced Raman scattering (SERS) has been developed to simultaneously detect trace quantities of multiple pathogenic antigens from West Nile virus, Rift Valley Fever virus and Yersinia pestis in fetal bovine serum. Antigens were detected by capture with silica-encapsulated nanotags and magnetic nanoparticles co...
Article
Full-text available
Rift Valley Fever virus (RVFV) is a mosquito-borne zoonotic virus that infects ruminants including cattle, sheep, goats, camels and buffalo. Multiplexing diagnostic assays that can simultaneously detect antibodies against multiple RVFV antigens offer a high throughput test for disease surveillance and vaccine evaluations. We describe the improvemen...
Article
Full-text available
Rift Valley fever phlebovirus (RVFV) causes high rates of abortions and fetal malformations in ruminants, and hemorrhagic fever, encephalitis, or blindness in humans. Viral transmission occurs via mosquito vectors in endemic areas, which necessitates regular vaccination of susceptible livestock animals to prevent the RVF outbreaks. Although ZH501 s...
Data
Virulence analysis of wild-type (wt) Rift Valley fever phlebovirus (RVFV) strains in mice. (A) Actual viral doses of wt RVFV strains; ZH501, Kenya 199800523, Kenya 90058, Saudi Arabia 200010911, OS1, OS7, SA75, Entebbe, or SA51. Percentages to the target dose (1x103 PFU) are also shown. (B–K) Clinical signs of disease observed in each mouse are sho...
Data
Sequence changes of wild-type Rift Valley fever phlebovirus (wt RVFV) and recombinant RVFV (rRVFV) stocks in comparison to reference sequences. (DOCX)
Data
Clinical signs of disease observed in mice infected with rZH501, rSA51, or rZinga strains. Clinical signs of disease observed in each mouse are shown in each group: (A) rZH501, 1x100 PFU, (B) rZH501, 1x101 PFU, (C) rZH501, 1x102 PFU, (D) rSA51, 1x100 PFU, (E) rSA51, 1x101 PFU, (F) rSA51, 1x102 PFU, (G) rZinga, 1x100 PFU, (H) rZinga, 1x101 PFU, and...
Data
Histopathological changes in livers of mice infected with pathogenic Rift Valley fever phlebovirus (RVFV) strains at 3 or 4 days post infection. Livers of mice intraperitoneally infected with 1x103 PFU of wt OS1 (A–C), wt Saudi Arabia 20010911 (D–F), rZinga (G–I), wt Kenya 199800523 (J–L), wt ZH501 (M–O), or wt SA75 (P–R) were histopathologically a...
Data
Northern blot analysis of recombinant Rift Valley fever phlebovirus stocks. Total RNA was extracted from Vero cells infected with each stock virus (Vero P1 of rescued P0 virus or Vero P2 of plaque clones) at 24 hours post infection. Northern blot used RNA probes specific to antiviral-sense (+) or viral-sense (-) L, M-, or S-segment RNA. (A) recombi...
Data
Northern blot analysis of wild-type (wt) Rift Valley fever phlebovirus stocks. Total RNA was extracted from Vero cells infected with each stock virus (parental stock, Vero P1 of parental stock, or Vero P2 of plaque clones) at 24 hours post infection. Northern blot used RNA probes specific to antiviral-sense (+) or viral-sense (-) L, M-, or S-segmen...
Data
Histopathological changes in spleens of mice infected with pathogenic Rift Valley fever phlebovirus (RVFV) strains at 3 or 4 days post infection. Spleens of mice intraperitoneally infected with 1x103 PFU of wt OS1 (A–C), wt Saudi Arabia 20010911 (D–F), rZinga (G–I), wt Kenya 199800523 (J–L), wt ZH501 (M–O), or wt SA75 (P–R) were histopathologically...
Data
Cross-neutralization of Rift Valley fever phlebovirus strains by sheep or cattle sera vaccinated with MP-12 or rMP12-ΔNSm21/384. Amino acid sequences of Gn (A) or Gc (B) proteins were compared among RVFV strains MP-12 (GenBank accession DQ380208), ZH501 (DQ380200), Kenya 9800523 (DQ380196), Saudi Arabia 200010911 (DQ380197), Zinga (DQ380217), OS1 (...
Article
Full-text available
Since 1999, 11 serotypes of bluetongue virus (BTV) similar to Central American or Caribbean strains have been isolated in the southeastern United States, predominantly in Florida. The majority of the incursive serotypes have remained restricted to the southeastern US. In recent years, BTV serotype 3 (BTV-3) has been isolated in areas increasingly d...
Article
A comparison of two geographicallly distinct viruses in the order Bunyavirales that are zoonotic and known to cause congenital abnormalities in ruminant livestock was performed. One of these viruses, Cache Valley fever virus, is found in the Americas and is primarily associated with disease in sheep. The other, Rift Valley fever virus, is found in...
Article
Full-text available
Rift Valley Fever (RVF) is a mosquito-borne zoonotic disease that presents a substantial threat to human and public health. It is caused by Rift Valley fever phlebovirus (RVFV), which belongs to the genus Phlebovirus and the family Phenuiviridae within the order Bunyavirales. The wide distribution of competent vectors in non-endemic areas coupled w...
Poster
Rift Valley Fever Virus (RVFV) is a mosquito-borne zoonotic pathogen endemic in parts of Africa and the Arabian Peninsula. RVFV causes acute febrile disease with mass abortion and death in ruminants and occasional encephalitis and death in humans. In the US, RVFV is a BSL-3Ag Select Agent and a foreign animal disease, requiring special containment...
Article
Full-text available
The complete genome sequence, including the untranslated regions, of two Rift Valley fever virus (RVFV) strains isolated from mosquitoes that were collected from disease outbreaks in Saudi Arabia (2001) and Kenya (2006 to 2007) were sequenced using next-generation sequencing technology.
Article
Rift Valley fever virus (RVFV), a Category A pathogen and select agent, is the causative agent of Rift Valley fever. To date, no fully licensed vaccine is available in the U.S. for human or animal use and effective antiviral drugs have not been identified. The RVFV MP12 strain is conditionally licensed for use for veterinary purposes in the U.S. wh...