Widiastuti Setyaningsih

Universitas Gadjah Mada | UGM · Department of Food and Agricultural Product Technology

An analytical pressurized liquid extraction (PLE) process has been studied for the extraction of phenolic compounds from rice grains. A fractional factorial design (27−2) with a centre point was used to optimize PLE parameters such as solvent composition (EtOAc in MeOH), extraction temperature, pressure, flushing, static extraction time, solvent-pu...

An analytical ultrasound-assisted extraction (UAE) technique has been optimized and validated for the extraction of melatonin from rice grains. A Box–Behnken design in conjunction with a response surface methodology based on six factors and three levels was used to evaluate the effects of the studied factors prior to optimizing the UAE conditions....

A new microwave-assisted extraction (MAE) method has been investigated for the extraction of phenolic compounds from rice grains. The experimental conditions studied included temperature (125–175 °C), microwave power (500–1000 W), time (5–15 min), solvent (10–90% EtOAc in MeOH) and solvent-to-sample ratio (10:1 to 20:1). The extraction variables we...

A new microwave-assisted extraction (MAE) method has been developed for the extraction of melatonin from rice grains. The stability of melatonin under MAE conditions was studied in order to define the working range. The studied analytical conditions for the MAE were temperature (125−175 °C), microwave power (500−1000 W), time (5−15 min), solvent (1...

Black glutinous rice (Oryza sativa var. glutinosa) tape fermented with various yeast, mold, and bacteria is often rich in phenolics compounds and can contribute positively to health through its antioxidants activity. Despite the potential, these compounds have limited bioavailability value due to their structure, degree of glycosylation or polymeri...

Rice (Oryza sativa) contains γ-oryzanol, which consists of 4 main compounds: Cycloartenol ferulate, 2,4-methylenecycloartanyl ferulate, campesterol ferulate, and β-sitosterol ferulate, that contribute to the health benefits of rice. This research aimed to develop a rapid method to determine the 4 major γ-oryzanol compounds in 45 varieties covering...

The application of Kappaphycus alvarezii in the food system has attracted researchers due to its bioactive compounds, including tryptophan. The present study was conducted to extract tryptophan from K. alvarezii with the aid of sonication. A reduced multilevel factorial design was conducted to evaluate the effect of solvent, ultrasound power, duty-...

Civet coffee, or kopi luwak, has attracted significant attention within the coffee industry in certain regions due to its distinct flavor characteristics that arise from the digestive processes of the civet. The ability to discriminate between wild and feeding civet coffee is of major importance in upholding the industry’s established standards of...

Roselle flower is an edible flower with health-beneficial bioactive compounds. In this study, red and purple roselle calyces were dried by foam-mat drying with different hydrophilic-lipophilic balance (HLB) of commercial emulsifiers as the foaming agents, including TBM, SP, and SSL. For a comparison is the roselle calyces powder from conventional d...

Kappaphycus alvarezii is one of the most essential edible seaweeds in the global trade of marine products. The product quality will directly influence the flavor, odor, and impact on the human health of this edible seaweed. These functions are associated with volatile organic compound (VOCs) content and are controlled by seaweed cultivation sites....

Chamomile has been widely used as a functional tea due to its effects on several neurohormones, some of which are related to tryptophan. An analytical ultrasound-assisted extraction (UAE) technique was successfully optimized and validated to determine tryptophan levels in chamomile flowers. A Box-Behnken design, in conjunction with response surface...

Young coconut mesocarp (YCM) contains considerable phenolic compounds that provide high antioxidant activity. However, the YCM is identified as an underutilized by-product in food industries. A novel functional food derived from YCM, viz., cookies, was proposed through this study to take advantage of the containing antioxidants. The cookies were pr...

Psidium laruotteanum is a subshrub Myrtaceae, native to the Brazilian savanna, and distributed in Central and South America. Here, the health beneficial compound piceid (reservatrol-3-β-mono-d-glucoside) is described for the first time in the genus Psidium. The ultrasound-assisted extraction from P. laruotteanum roots was optimized by a response su...

This study aimed to determine the optimal UAE conditions for extracting anthocyanins from pigmented corn using the Box–Behnken design (BBD). Six anthocyanins were identified in the samples and were used as response variables to evaluate the effects of the following working variables: extraction solvent pH (2–7), temperature (10–70 °C), solvent comp...

Edible flowers, including roselle, contain a varied composition of phenolic compounds that may inhibit the oxidative stress mechanism. Roselle-based tisane with appealing sensory properties is commonly consumed worldwide. However, the conventional hot-brew method may ruin the stability of thermolabile phenolic compounds during the tisane preparatio...

Civet coffee is the world’s most expensive and rarest coffee bean. Indonesia was the first country to be identified as the origin of civet coffee. First, it is produced spontaneously by collecting civet feces from coffee plantations near the forest. Due to limited stock, farmers began cultivating civets to obtain safe supplies of civet coffee. Base...

Coriander (Coriandrum sativum L.) is among the most widely used medicinal and aromatic plants. It is well known for its multiple health benefits, most of which are correlated with its phenolic composition. Four phenolic compounds were identified in the extracts of aerial parts of coriander extracts, including caffeic acid, isoquercitrin, quercetin-...

A novel analytical method based on microwave-assisted extraction has been successfully optimized and validated to determine resveratrol from functional marmalade and cookies. The optimization was performed using a Box–Behnken design with three factors: solvent composition (60−100% and 10−70% methanol in water for marmalade and cookies, respectively...

Macroalgae are an essential basic ingredient for many industries from which numerous derived products with great economic value are manufactured. Each macroalga has a unique composition that might provide specific physical and chemical information that can be used as markers for authentication. Their compositions may differ depending on different f...

Nowadays, dried edible flowers have become one of the eating habits of a healthy lifestyle. The most common way to consume dried flowers is via infused water (tisane). A number of studies on dried edible flowers have reported antioxidant activities mainly due to their phenolic compounds. This work has developed a new extraction method using ultraso...

D-psicose is a rare sugar, used as an alternative to the natural sweetener that provides 70% of sucrose’s sweetness, with low-calorie absorption in the human body. Considering the health-beneficial effect and high availability of D-psicose in raisins (the world’s most consumed dried fruit), it is of interest to establish a reliable analytical metho...

Coconut (Cocos nucifera) fruit has attracted consumer interest due to its health benefits, especially the consumption of coconut water produced from 6–7-month-old coconut fruit. Consequently, by-products from young coconut fruit are also being expanded, including coconut mesocarp containing phenolic compounds. Therefore, this study aimed to provide...

Coconut is widely used as a food source in producing countries, and during consumption, the waste that is generated needs to be reduced through by-products processing to ensure environmental sustainability. This study aimed to assess the functionality of by-products (endo- and mesocarp) of coconuts at early and mature stages. The aqueous and ethano...

This research aimed to develop and validate a reversed phase-high performance liquid chromatography method to determine phenolic compounds in dried flowers extract simultaneously. The research was divided into two parts: (1) optimization of the separation condition employing a Box Behnken design, and (2) validation test including assessment for the...

Red fruit oil (RFO) is a high-value oil that contains functional compounds, mainly phenolic compounds, providing antioxidant activity. Therefore, an optimal extraction method is essential to recover the RFO and phenolic compounds simultaneously. This research aimed to optimize the ultrasound-assisted extraction (UAE) for oil from red fruit using th...

The brewing properties of coffee products are defined by the chemical composition in the bean, including sugars and polyols. Some factors, such as coffee species and roasting, may affect the level of these compounds in the bean. A new analytical microwave-assisted extraction (MAE) method has been developed to extract sugars and polyols from the cof...

Kulit koro pedang putih dapat digunakan sebagai sumber selulosa. Salah satu alternatif untuk meningkatkan aplikasi selulosa adalah dengan memodifikasi selulosa menjadi produk turunan selulosa yaitu Hydroxypropyl Methyl Cellulose (HPMC). Tujuan dari penelitian ini adalah melakukan optimasi terhadap sintesis dan karkaterisasi HPMC dari selulosa kulit...

Phenolic compounds are bioactive compounds that are naturally found in red algae. To determine the level of these compounds in the red algae, spectroscopic or chromatographic determination was applied over the liquid extracts. Therefore, a prior extraction method is needed. The presented study aimed to develop the analytical ultrasound-assisted ext...

The algae Kappaphycus alvarezii is considered an important raw material for industrial practices, producing high economic value of various derived products. However, the quality of this commodity, which can be indicated by the level of phenolic compounds, may vary due to growth factors, including cultivation sites. An analytical UV-Vis spectrophoto...

The pandemic situation experienced by the world are currently threatening food security in various regions including Indonesia. In these regions, the flow of traffic in the agricultural and fisheries sectors, from the means of production to the distribution of the harvest, is choked up due to social restrictions in various parts of the region. As a...

Jack bean (Canavalia ensiformis), Indonesian local bean, has a high content of protein and great potential either for substituting or overcoming the shortage of imported soybean in tofu production. However, an appropriate coagulant in a proper amount is needed to make tofu with acceptable characteristics. This study set out to investigate the appro...

Abstract Porang glucomannan is a polysaccharide extracted from Amorphophallus oncophylus and its degradation product is porang oligo-glucomannan (POG). It contains glucomannan, which has been used as an emulsifier, thickener, or as a nutritional supplement to counter many diseases. While it is believed to have health benefits, there are only limite...

Sidaguri (Sida rhombifolia) is one of the herbal components used in traditional medicine. The application of chemometrics in the standardization of herbal medicine is common. The objective of this study was to classify Sidaguri from different regions based on FTIR spectra with chemometrics of principal component analysis (PCA) and to correlate the...

Betacyanins have been reported to provide prominent health-promoting effects, in addition to contributing to the exotic color and pleasant palate of red dragon fruit that has been attracting universal interest for over a decade. An accurate determination of betacyanins in dragon fruit is important if we are to benefit from the nutraceutical feature...

Sweeteners have been used in food for centuries to increase both taste and appearance. However, the consumption of sweeteners, mainly sugars, has an adverse effect on human health when consumed in excessive doses for a certain period, including alteration in gut microbiota, obesity, and diabetes. Therefore, the application of non-nutritive sweetene...

Edible flowers have been widely consumed for ages until now. The attractive colors and shapes, exotic aroma, and delightful taste make edible flowers very easy to attain. Moreover, they also provide health benefits for consumers due to the unique composition and concentration of antioxidant compounds in the matrices. Knowing the bioactive compounds...

An new method for the extraction of phenolic compounds from olive oil has been proposed before the chromatographic analysis by UPLC-PDA (Ultra-Performance Liquid Chromatography-PhotoDiode Array). Recoveries for 20 individual phenolic compounds were compared using three cartridges of solid-phase extraction including C-18, Diol, and DVB (DiVinylBenze...

Most starch industries utilize maceration as the extraction method to recover starch from corn matrices; however, the production is time-consuming and often inefficient. Ultrasound-assisted extraction (UAE) appears to propose an enhancement of the extraction process. Hence, in this study, UAE has been developed to reach an optimum recovery in a sho...

Roselle (Hibiscus sabdariffa L.), as an edible flower, has long provided an array of positive effects on human health. This benefit is a result of phenolic compounds that are naturally present mainly in the calyx. Plentiful medicinal remedies and functional foods based on this flower are available worldwide, as supported by the studies of phenolic...

The growing demand for Spirulina (Arthrospira platensis) as a dietary supplement suggests that a rapid and reliable method to determine its saccharides and polyols content would be of great interest. Additionally, the impact of these microalga growing conditions may have on such content would be really useful for Spirulina producers. This manuscrip...

Porang (Amorphophallus oncophyllus) is an indigenous tuber of Indonesia that rich in glucomannan. An alternative approach to produce porang oligo-glucomannan (POG) as prebiotic from porang glucomannan (PGM) was made by enzymatic hydrolysis using β-mannanase. This study aimed to produce POG under optimal conditions by controlled enzymatic hydrolysis...

Ultrasound-assisted extraction (UAE) has been optimized to improve the current cassava starch production by conventional maceration for the extraction method. Evaluation of several extraction parameters disclosed significant effects (p < 0.05) by three studied factors (ultrasound power, x1; pulse duty-cycle, x2; and solvent to sample ratio, x3). Su...

The acid dissociation constant (pKa) is the fundamental physicochemical properties required to understand the structure and reactivity of boronic acid-based material as a sensor that identifies carbohydrates. However, there is a lack of comprehensive study on the impact of the substituent on the pKa of monosubstituted phenylboronic acid in aqueous...

Patin fish oils (PFOs) from fifteen different origins was evaluated the antioxidant activity using 2,2’-diphenyl-1-picrylhydrazil (DPPH) radical scavenging. All PFO samples were analyzed using Fourier Transform Infrared (FTIR) spectroscopy. The spectra were recorded in absorbance mode and processed statistically using multivariate chemometric techn...

Phenolics are bioactive compounds already reported in Hibiscus sabdariffa (roselle). The present study reports the development and validation of the analytical microwave-assisted extraction (MAE) method for the determination of phenolic compounds in roselle flowers to establish their positive contribution to human health. Prior to the optimization,...

Electrochemical biosensors have attracted a tremendous attention for many researchers recently due to its facile synthesis process, tunability easiness by tailoring the material properties or composition, and wide range of biological analyte types detection. To obtain an excellent electrochemical biosensor performance, a material that facilitates f...

The usage of food additives must respect the general legislation in force in the country and requires a reliable analytical method for surveillance. This research aimed to develop a high-performance liquid chromatography with diode array detection (HPLC-DAD) method for the simultaneous determination of seven food additives and caffeine in powdered...

Mangosteen, or Garcinia mangostana L., has merged as an emerging fruit to be investigated due to its active compounds, especially xanthone derivatives such as α -mangostin (AM), γ-mangostin (GM), and gartanin (GT). These compounds had been reported to exert some pharmacological activities, such as antioxidant and anti-inflammatory, therefore, the d...

The present research intends to develop a new method based on headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) for the detection and determination of patin fish oil adulterated with different percentages of palm oil, because it is a cheaper vegetable oil. Five variables that affect headspace generation (incubation time and tempera...

Authentication of Patin fish oil (MIP) is essential to prevent adulteration practice, to ensure quality, nutritional value, and product safety. The purpose of this study is to apply the FTIR spectroscopy combined with chemometrics for MIP authentication. The chemometrics method consists of principal component regression (PCR) and partial least squa...

Objective: This study aimed to evaluate the antidiabetic and antidepressant effects of banana peel flakes in streptozotocin-induced diabetic rats. Methods: Twenty-five male Wistar rats were classified into five groups with different treatments. Groups I to IV were diabetic rats model groups that consumed only standard diet, standard diet containing...

Rice, especially the pigmented varieties, contains a higher level of melatonin (N-acetyl-5- methoxytryptamine), which is used in treatment for sleeping disorders and for improving mood. It has potent antioxidant properties, anti-obesity, and anti-inflammatory, thus potential as the basis for functional food development. The purpose of the study was...

The growing interest in spicy foods leads to the global demand for spices, particularly dried chili. This study aimed to assay both aflatoxin (AFs) and ochratoxin A (OTA) contamination using an integrative method of morphological identification, molecular detection, and chromatography analysis on dried chili provided from traditional and modern mar...

Joruk is a traditional fishery product made by spontaneous fermentation process using lactic acid bacteria by salting method, followed by fermentation process. Hydrolysis of proteins with the pepsin and a combination of pepsin and trypsin enzymes by in vitro can produce bioactive peptides. The aims of this study were to investigate the change of so...

The aroma of rice essentially contributes to the quality of rice grains. For some varieties, their aroma properties really drive consumer preferences. In this paper, using a dynamic headspace solid-phase microextraction (HS-SPME) system coupled to a two-dimensional gas chromatography (GC×GC) using a time-of-flight mass spectrometric detector (TOFMS...

Both glycerolysis and the interesterification reactions take place in one reaction system. The objectives of this research were to obtain chicken stearin and to evaluate the effect of solvent to fat ratios and chicken stearin to palm stearin ratios on total mono-acylglycerol (MAG) and diacylglycerol (DAG) of product by the chemical glycerolysis-int...