Widanarni Widanarni

• Dr.
• Head of Department at IPB University

In the pangasianodon aquaculture industry at Indonesia, vaccination is considered a backup plan for treating the enteric septicemia of catfish (ESC) diseases. The varied phenotypic and genotypic features of Edwardsiella ictaluri isolates from different aquaculture locations in Indonesia can be used to discover potential vaccine formulations that of...

Forest onion potentially acts as an immunostimulant, enhancing the immune response of tilapia to pathogen infection. This study aimed to evaluate the effectiveness of forest onion simplisia on growth performance, immune response, and resistance of tilapia to Streptococcus agalactiae infection. The research was divided into in vitro and in vivo test...

Vibrio harveyi is one of the common bacteria that cause disease in vaname shrimp. The purpose of this study is to determine the efficacy of bacterial metabolites Proteus myxofaciens JB 20B as an antibiofilm agent in the prevention and treatment of V. harveyi-infected vaname shrimp (Litopenaeus vannamei). The research design included eight treatment...

The production of shrimp has been challenged by various factors such as diseases. Therefore, the health of cultured shrimp is essential for successful farming practices. The application of medicinal plants brings about numerous benefits in aquaculture including simplicity in usage, biodegradability, and minimal environmental impact over extended pe...

Probiotics are believed to contain active components that affect fish reproduction. This study aimed to evaluate the metabolite compounds contained in the probiotic Bacillus sp. NP5 and its effect on female catfish broodstock rematuration. The first step was to analyze the metabolite content of probiotic using liquid chromatography with tandem mass...

The intensive system is expected to increase production and productivity in white shrimp cultivation. However, several latent variables such as social, economic and technical aspects have little known influence on shrimp production performance and productivity. This research aimed to evaluate the path analysis model factors that influence productio...

Kualitas sumber daya manusia menjadi faktor kunci keberhasilan budidaya. Petambak yang memiliki pengetahuan dan keterampilan teknis yang baik cenderung lebih berhasil dalam mengelola tambaknya. Oleh karena itu, melalui program pelatihan dan pendampingan, diharapkan dapat memberikan pengetahuan dan keterampilan yang diperlukan untuk meningkatkan pro...

Jaundice is a disease that can affect catfish, resulting in low or unsalable selling prices. The yellow colour in catfish is associated with tissue/organ disorders, particularly bile or liver function. This study aims to compare the histopathological structure of the liver, kidney, intestine, spleen, and bile of naturally jaundiced catfish and thos...

This study aimed to evaluate the inhibition of V. parahaemolyticus infection in the whiteleg shrimp (Penaeus vannamei) reared in different volumes of biofloc system. Post-larval shrimp with an average body weight of 0.28 ± 0.01 g were reared in 15 aquariums with working volume of 33.3 L and stocking density of 3 shrimp per liter. The shrimp were re...

Probiotics have become an alternative in the aquaculture industry and are considered an environmentally friendly approach for disease control. However, the maintenance of the viability of probiotic bacterial cells during storage is quite challenging. This study aimed to investigate the potential effects of euryhaline probiotic and paraprobiotic B....

Vibrio parahaemolyticus infection is a major threat to Pacific white shrimp aquaculture, causing significant shrimp mortality. Bacteriophages (phages) provide a promising antibiotic alternative due to their specificity towards specific bacteria. This research includes essential steps isolating phages from shrimp pond water and characterizing them,...

Catfish Clarias sp. is one of the leading commodities in demand and has economic value. Low survival due to cannibalism and disease is a serious problem. To overcome these problems is application of bioflocs and probiotics Bacillus NP5. This study aimed to analyze the appropriate dose of Bacillus NP5 probiotic addition to the biofloc system to impr...

Vibrio parahaemolyticus is one of the pathogens in crustaceans that can cause mass death in vannamei shrimp farming. This study aimed to evaluate the effect of administering the commercial herbal supplement Phycurma Aquatic (PA) through feeding at different doses to prevent V. parahaemolyticus infection in vannamei shrimp. This study used a complet...

The use of probiotics has become an alternative in the aquaculture industry and is considered a new tool for disease control. However, the maintenance of viability of probiotic bacterial cells during storage is quite challenging. This study aimed to investigate the potential effects of euryhaline probiotic and paraprobiotic B. cereus BR2 on African...

The present research studies the effects of kebar grass extract on the growth and enhancement reproduction of male broodstock catfish. Employing three distinct methods of administration ethanol extract injection, the simplicia method, and traditional extraction this research assesses their impact on the test fish development and survival. The resul...

Probiotic administration has the ability to increase broodstock performance and aquaculture productivity. The effect of dietary probiotics Bacillus cereus NP5 on the reproductive performance of female Clarias gariepinus and the survival of the resulting larvae was investigated in this study. Female fish with an initial weight of 553 ± 27.41 g, were...

Immunostimulants represent the most innovative approach for combating shrimp diseases. They are molecules that effectively enhance the host's nonspecific defenses against invading microorganisms. However, methodological differences exist among immunostimulants based on the same source. Therefore, conducting a meta-analysis is essential to derive va...

This study aimed to evaluate the effectiveness of synbiotic microcapsules through feed on growth performance and immune response of Pacific white shrimp (Litopenaeus vannamei) in field testing during IMNV disease outbreaks and fluctuating weather. Four groups of shrimps (4.11 ± 0.05 g) with four replicates were fed with different doses of synbiotic...

This study aimed to evaluate and unveil the positive impact of biofloc culture on Vibrio parahaemolyticus infection of Pacific white shrimp by reducing quorum sensing (QS) and virulence gene expression and enhancing shrimp's immunity. The shrimp with an average body weight of 0.50 ± 0.09 g were reared in containers with a volume of 2.5 L, 21 units,...

The Pacific white shrimp Litopenaues vannamei is the most widely cultivated shrimp in the world, particularly in Indonesia. Vibrio parahaemolyticus is a bacterial pathogen that severely affects shrimp, causing extensive economic losses in L. vannamei culture. This study is the first report of the effects on the L. vannamei intestine after Vibrio pa...

The aim of this study was to evaluate single and multispecies probiotic applications for the prevention of Motile Aeromonads Septicaemia (MAS) disease in gourami, Osphronemus gourami. The experiment consisted of the in vitro inhibition test and the in vivo application of probiotics in gourami. The in vivo assay, consisted of five treatments and fiv...

Spiny lobster is a member of crustaceans that has high economic value. The demand for export markets in Asian, European and American countries as well as locally, is quite high with an expensive selling price. However, there are obstacles that often occur in spiny lobster rearing cultivation, namely infectious diseases that will cause high mortalit...

Biofloc technology has been shown to have a positive impact on shrimp culture by controlling pathogenic Vibrio. This study aimed to analyze the effect of biofloc on the Vibrio composition in water and shrimp gut, as well as the growth performance and microvilli of Pacific white shrimp (Penaeus vannamei). Shrimp post-larvae measuring 0.66 ± 0.02 g w...

This study aims to evaluate the effectiveness of plants and silica in Tor soro fish populations vaccinated with Aeromonas hydrophila antigen. Tor soro fingerlings with a size of 3.4 ± 0.15 cm were stocked in an aquarium measuring 50×30×30 cm with a density of 50 fish per aquarium. The study used a completely randomized design with 4 treatments, i.e...

This study aimed to evaluate the effectiveness of synbiotic microcapsules through feed on growth performance and immune response of vaname shrimp in field testing during IMNV disease outbreak and fluctuate weather. Four groups of shrimp(4.11±0.05g) were fed with different doses of synbiotic microcapsules, SIN 0(0%), SIN 0.5(0.5%), SIN 1(1%) and SIN...

Cobia is a relatively new species of marine carnivorous fish with high aquaculture potential because of its fast growth (4-6 kg in a year) and ability to adapt and reproduce in the cultivation environment. This study aims to evaluate the nutrient digestibility and enzyme activity of cobia juveniles fed pelleted feed with microbial floc meal (MF), m...

Evaluation of human resource development and institutional aspects is a process of providing information that can be used as a consideration to improve capabilities and be able to influence the implementation of community empowerment programs. The aimed of this study was to evaluate the capacity of human resources, institutions, and aquaculture mar...

Dayak onion (Eleutherine bulbosa (Mill.) Urb.) has the potential to be an immunostimulant to benefit fish health. This study aimed to evaluate the effectiveness of dietary supplementation with the powder and crude ethanol extracts of Dayak onion on the hematology and immune response parameters of Nile tilapia challenged with Aeromonas hydrophila. T...

Canarium indicum offers the potential of usage in aquaculture. This study aimed to evaluate the utilization of C. indicum on growth performance, health, and resistance of Asian seabass Lates calcalifer challenged with Vibrio alginolyticus. The study employed a completely randomized design with seven treatments, namely positive control (K+), negativ...

Aeromonas hydrophila is the main causative agent of ulcerative disease in catfish and causes considerable economic losses to Indonesian aquaculture. This study evaluates the prebiotic activity and the effect of feed supplementation of dayak onion simplicia powder (DOSP) on the immune response and survival of catfish infected with A. hydrophila. Fiv...

This study aimed to identify the fish health status, based on the total erythrocytes (cells/mm3), total leukocytes (cells/mm3), hemoglobin level (g %), hematocrit, and histopathological profiles. This study was performed on February, 2021 – January, 2022. The study was located in floating-net cage of Kelapa Dua Island, Seribu Islands, Jakarta, Indo...

Evaluation of human resource development and institutional aspects is a process of providing information that can be used as a consideration to improve capabilities and be able to influence the implementation of community empowerment programs. The aimed of this study was to evaluate the capacity of human resources, institutions, and aquaculture mar...

Cantang grouper is a hybrid grouper which is one of the main grouper species cultivated in Indonesia. This study aimed to evaluate the growth and health status of cantang grouper in floating net cages supplemented with clove powder. This study used a completely randomized design with three treatments, each with three replications. Two levels of clo...

This study aimed to evaluate the different effects of dietary microencapsulated synbiotics on the growth, intestinal histological, immune responses, and gut microbiota diversity of white shrimp ( Penaeus vannamei ). This study used a completely randomized design with five treatments and three replications. Five different feed treatments use to feed...

Highlight Research The dose, duration of immersion, and time interval of immersion of the estradiol-17β affect the rate of cannibalism and increase the seed survival of Asian redtail catfish in low treatment in this research. Over optimum treatment increased the incidence of cannibalism at Asian redtail catfish and decrease growth performance. Trea...

Vibrio alginolyticus which becomes the main cause of vibriosis disease in grouper culture and causes great economic loss in Asian aquaculture industries. This study investigated the antibacterial activity of clove extract and the effect of adding clove powder to the diet on survival cantang grouper infected with V. alginolyticus. The clove extracti...

The aim of the study is to test the effectiveness of adding various paraprobiotic doses of Bacillus sp. NP5 through feed in improving the immune responses and resistance of tilapia juvenile (Oreochromis niloticus) to infection of Streptococcus agalactiae. Bacillus sp. NP5 paraprobiotic was produced through heat-inactivation at 95°C for 1 h. This st...

Aquaculture with an intensive system can both increase production and the disease risk. The use of antibiotics to reduce pathogens has bacterial resistance consequence. Peptides have rapidly gained more attention due to their antibacterial and immunomodulatory properties important to combat pathogens. This study aimed to determine the effectiveness...

This study aimed to evaluate the application of synbiotic containing multispecies of probiotics with different cell densities in white shrimp rearing against infectious myonecrosis virus (IMNV) and Vibrio parahaemolyticus coinfection. This study used a completely randomized design with five treatments and three replications. One additional replicat...

P. piscicida 1Ub and FOS were evaluated for their potential synbiotic effects on growth, immunological responses, and disease resistance against white spot syndrome virus and V. harveyi coinfection, the major pathogen in whiteleg shrimp aquaculture. Four different supplemented diets were used to feed the experimental shrimp for 40 days: control (co...

This study aimed to evaluate the effectivity of microencapsulated synbiotic (MS), Bacillus sp. NP5 and mannan oligosaccharide (MOS) dietary in different feeding frequencies in Pacific white shrimp culture field experiment. The MS was administered as a feed supplementation to enhance the immunity for prevention against co-infection with WSSV (White...

Fructooligosaccharides (FOS) are natural feed additives with potential application in feed for Pacific white shrimp. This study aimed to evaluate the effects of prebiotic FOS supplementation on the modulation of metabolism-related gene expression and growth performance of Pacific white shrimp. A trial feed consisted of commercial feed supplemented...

Oksitetrasiklin banyak digunakan dalam manajemen terapeutik maupun preventif infeksi penyakit bakterial pada akuakultur. Konsentrasi obat yang tepat dalam tubuh penting untuk kemanjuran terapi tidak hanya ditentukan oleh dosis obat tetapi juga farmakokinetik obat yang dapat diketahui dari parameter farmakokinetiknya. Parameter farmakokinetik melipu...

This research aimed to study the pathogenesis of Aeromonas hydrophila infection through two different routes of infection in African catfish and to find out the involvement of TNF-a in response to the infection. The experimental infection model was performed by clipping the caudal fin and immersing the fish in a medium with A. hydrophila and by int...

Heat-shock protein 70 (HSP70) is a member of the Heat-shock protein family that plays an important role in cellular homeostasis against various stressors in fish. This research aimed to identify the full length of cDNA sequence HSP70 in African catfish C. gariepinus and assessed its mRNA expression under various tissues and stress conditions. The c...

Motile Aeromonad Septicemia (MAS) is caused by Aeromonas hydrophila and often attacks juvenile catfish. Probiotics could be an alternative to prevent MAS disease. This study aims to determine the dose of probiotics to improve the survival, growth performance, and immune response of juvenile catfish Pangasianodon hypophthalmus to the A. hydrophila i...

Motile Aeromonad Septicemia (MAS) is caused by Aeromonas hydrophila and often attacks juvenile catfish. Probiotics could be an alternative to prevent MAS disease. This study aims to determine the dose of probiotics to improve the survival, growth performance, and immune response of juvenile catfish Pangasianodon hypophthalmus to the A. hydrophila i...

Cannibalistic behavior of Asian redtail catfish is a quite significant obstacle in cultivation, especially in the hatchery phase. The purpose of this study was to evaluate intracohort cannibalism of Asian redtail catfish fry and the way of predation among them at high stocking densities. The rearing method used a completely randomized design with 4...

White feces disease (WFD) recently appeared in white shrimp (Penaeus vannamei) production. Researchers conducted several studies to analyze the differences in gut microbiota between healthy and diseased shrimp, especially in the case of WFD. This study was conducted to compare the diversity and relative abundance of intestinal microbial communities...

This research was conducted to find the best combination of coating material and microencapsulation method for synbiotics. This research consists of 3 chapters. Chapter 1, was conducted to obtain the best combination of 3 types of coating materials (whey protein, skim milk, and maltodextrin) with 3 doses of coating material (0%, 10%, and 20%). Chap...

The mitigation of vibriosis in shrimp culture could be accomplished by reducing the virulence of the pathogen or by increasing the immune response of the shrimp. This study aims to evaluate the application of a biofloc system in protecting Pacific white shrimp (Penaeus vannamei) from pathogenic Vibrio parahaemolyticus infection. Shrimp post-larvae...

This study aimed to compare the phenotypic and genotypic characteristics, and pathogenicity of Edwardsiella ictaluri derived from several catfish farming in Indonesia. The isolated bacteria were from West Java, East Java, South Sumatra, Jambi, Lampung, Riau and South Kalimantan. The phenotypic analysis based on conventional biochemical characterist...

The dietary supplementation of probiotics Bacillus sp. NP5 in female African catfish Clarias gariepinus (Burchell 1822) resulted in the improvement of the reproduction performance of the fish in one of our previous studies, but its effects on the male fish have not been evaluated yet. Thus, this study aimed to evaluate the effect of dietary Bacillu...

Vibriosis is a major bacterial disease in shrimp farming, which is caused by Vibrio spp. It has resulted in substantial shrimp mortality. One strategy to control this disease is to use microcapsules containing marine bacteria. This study was established to evaluate the effectiveness of such microcapsules in controlling vibriosis in white shrimp. Th...

Mycobacteriosis has been recognized as an infectious disease caused by Mycobacterium fortuitum in aquaculture. In Indonesia, mycobacteriosis outbreak has been detected in West Java, Central Java, and East Java. However, no studies have yet described the pathogenesis of mycobacteriosis in gourami. Therefore, this study was designed to detect the por...

This study aimed to evaluate the effects of banana (Musa paradisiaca) midrib extract supplementation as preventive and curative treatments of motile aeromonad septicemia (MAS) on giant gourami (Osphronemus gouramy). Disc diffusion assay was conducted to evaluate antibacterial activity of banana midrib extract against Aeromonas hydrophila. The most...

Bawang hutan Eleutherine bulbosa (Mill.) Urb.merupakan tanaman obat yang memiliki kandungan oligosakarida, berpotensi sebagai prebiotik yang mampu meningkatkan kesehatan usus udang vaname (Litopenaeus vannamei). Tujuan dari penelitian ini adalah menguji efek dari ekstrak bawang hutan dengan konsentrasi berbeda terhadap perubahan pH media pada stimu...

Shrimp farming intensification has impacted various diseases; one of these is white faeces disease (WFD). Factors that cause WFD remain unknown. The research analysed phytoplankton and bacteria abundance and community composition in intensive Pacific white shrimp farming during an outbreak of WFD. Composite sampling was conducted. Fifty litres of w...

This study aimed to evaluate the influence of microalgae addition on the physical and functionality of bioflocs and the growth and health performance of giant prawn (Macrobrachium rosenbergii). Three biofloc‐based giant prawn culture systems were compared that is biofloc system without microalgae addition as a control, biofloc system with Chlorella...

An effort to increase the production of juvenile catfish is limited by the high mortality rate, especially caused by cannibalism. The hormonal treatment has been conducted as an effort to control cannibalism. This study used completely randomized factorial design, consisted of six treatments and three replications. There were two factors examined i...

Probiotics have become necessarily for aquaculture activities due to their important role in enhancing production. Probiotics, such as bacteria B. megaterium PTB 1.4 and P. pentosaceus E2211, are well known enabling to increase the growth and feed consumption of catfish. The aim of this study was at evaluating the administration of combining B. meg...

The availability of seawater for farming is limited to certain locations, therefore, Penaeus vannamei is often cultured in places with low-salinity media. The objective of this study was to evaluate sedimentary waste nutrients, water quality and production profiles of P. vannamei reared in low salinity ponds. Data were obtained for different rearin...

This study was aimed to evaluate the effectiveness of Bacillus sp. NP5 paraprobiotic administration through commercial feed on growth performance, immune response, and resistance of Nile tilapia against Streptococcus agalactiae infection. Bacillus sp. NP5 paraprobiotic was produced through heat-inactivation at 95°C for 1 h, then performed a viabili...

The decline in production has prompted researchers in various countries to continue to study factors supporting the success of Holothuria scabra culture. This research aims to evaluate the characteristic of nutrients, size of a sand fraction of the sediment from natural habitats, and the digestive tract, as a basis to determine the grain size of sa...

Green catfish is a high economic value commodity in Riau. However, cannibalism in green catfish seeds is still an obstacle in its hatchery, and so far there has been no specific study of cannibalism in green catfish. The purpose of this research was to study the cannibalism of the green catfish seeds at different stocking densities. The research us...

The present study aimed to evaluate the growth performance, immune response, and immunity-related genes in white shrimp given the Bacillus sp. (NP5) probiotic and honey prebiotic and V. parahaemolyticus infection. This study consisted of five treatments: NP5, honey, synbiotic (honey and NP5), positive control (PC), and negative control (NC), respec...

Asian sea bass is one of economically important commodities in aquaculture. However, its culture often challenged by vibriosis infection which resulted in lower production. The study aimed to evaluate the effectiveness of the ambon banana plant powder application in feed as an effort to control the disease caused by Vibrio alginolyticus infection....

The production of catfish in aquaculture is still limited by the low supply of good quality seeds. One solution that can be done to overcome this problem is by the application of biofloc technology with microalgae addition. This study aims to evaluate the performance of digestive system, growth and robustness of the African catfish larvae maintaine...

Sebagai sebuah entitas akademik dan sebagai pemikir, DGB (Dewan Guru Besar) IPB University memformu-lasikan suatu gagasan tentang Kota Tepian Air Ramah Lingkungan yang dirajut dalam pengelolaan sum-berdaya air berkelanjutan, yang dapat diterapkan pada sejumlah kota di tanah air baik yang berada di tepi sungai, tepi danau, tepi rawa, maupun di tepi...

Shrimp pond sediment (SPS) contains a significant quantity of nutrients. The use of SPS as a source of nutrients could prevent the adverse effects of the sediment on the water's ecosystem. The objective of this study was to evaluate the performance and physiological responses of Holothuria scabra fed with SPS. Five substrate treatments were prepare...

Prebiotic administration is one method to increase growth performance, improve intestinal microbiota composition, inhibit pathogen growth and induce immunity in fishes. Honey consists of oligosaccharides as the major content that can be used as a prebiotic. This study investigated the effects of administration of dietary prebiotic from honey at dif...

This study aimed to evaluate the growth performance and intestinal microbiota composition in Pacific white shrimp after probiotic, honey prebiotic, or synbiotic treatment. Pacific white shrimp were treated for 45 days with probiotic (1% (v/w) of Bacillus sp. NP5 RfR probiotic), prebiotic (0.5% (v/w) of honey prebiotic), synbiotic (1% (v/w) of probi...

White spot disease caused by White Spot Syndrome Virus (WSSV) is the most serious viral disease and has a major impact on the decline in production of white shrimp farm. Improving the immune response through the application of prebiotic, probiotic and synbiotic is expected to be one of the environmentally friendly alternatives to prevent the diseas...

The intensification of catfish culture requires a regular and sustainable good seeds quality. Microbial-based aquaculture with probiotic application is expected to improve the reproductive performance of catfish. The aim of this study was to evaluate the reproductive performance of catfish given probiotics Bacillus sp. NP5 with different concentrat...

Motile aeromonad septicaemia (MAS) in catfish can be done by improving the immune responses through probiotic administration. Co-administering probiotics producing digestive enzymes are expected to have an impact on fish growth. This study was aimed to evaluate the effectivity of probiotics B. megaterium PTB 1.4 and P. pentosaceus E2211 to improve...

Fermentation liquid from mangrove leaves Avicennia marina contains microorganisms, nutrients, and secondary metabolites. This study aimed to identify bacteria and the compounds in fermentation liquid of mangrove leaves A. marina and measured their inhibitory capacity against pathogenic bacteria Stenotrophomonas maltophilia which causes ice-ice dise...

Raharja NI, Widanarni, Wahyudi AT. 2019. Marine sponge-associated bacteria as biocontrol agents of vibriosis on whiteleg shrimp caused by Vibrio parahaemolyticus. Biodiversitas 20: 3164-3169. Sponge-associated bacteria are commonly known as an excellent source of microbial's bioactive compounds attributed to biological properties, including antibac...

This study aimed to produce microencapsulated probiotic Pseudoalteromonas piscicida (1Ub) and evaluate it with preb­iotic mannan-oligosaccharide (MOS) through the enrichment of Artemia sp., on bacterial population, growth performances, immune responses, and disease resistance of Pacific white shrimp larvae. Microencapsulation of probiotic was done...

Ornamental fish is non consumption fish which is an important source of Indonesian foreign exchange. The objective of this study is to analyze the productivity of bronze corydoras Corydoras aeneus ornamental fish through increased stocking density with biofloc technology. The average weight of the experimental corydoras was 0.61 ‒0.72 g with 2.32‒2...

White spot disease (WSD) outbreaks due to white spot syndrome virus (WSSV) infection cause the decline of the global shrimp production. The alternative prevention method against WSSV infection can be done by the improvement of immune responses through the application of honey as a prebiotic. This study aimed to evaluate the effectiveness of the adm...

The intensive culture system of white shrimp with high stocking density leads to slow shrimp growth performance. An alternative way to increase the growth performance of white shrimp is using a prebiotic. This study was aimed to evaluate the effectiveness of honey as a prebiotic through commercial feed to improve the growth performance of white shr...

Penerapan sistem budidaya intensif seringkali dihadapkan pada penurunan performa pertumbuhan dan kejadian infeksi penyakit. Upaya untuk meningkatkan status kesehatan ikan agar dapat tumbuh dengan baik dan tahan terhadap serangan penyakit sangat diperlukan. Penelitian ini dilakukan untuk mengevaluasi pengaruh pemberian probiotik Bacillus sp. ND2 dan...

The present study was aimed to evaluate the effectiveness of administration of honey as a prebiotic in improving growth performance, immune response, and resistance of Pacific white shrimp Litopenaeus vannameito to Vibrio parahaemolyticus infection. In the present study, the bee honey added to practical diet at levels of 0.0%, 0.25%, 0.5%, 0.75%, a...

This study aimed to evaluate the immune response and the disease resistance of Pacific white shrimp (Litopenaeus vannamei) larvae administered probiotic Pseudoalteromonas piscicida 1Ub 10⁶ CFU mL⁻¹, prebiotic mannan-oligosaccharide (MOS) 12 mg L⁻¹, and synbiotic (probiotic P. piscicida 1Ub 10⁶ CFU mL⁻¹ and 12 mg L⁻¹ prebiotic MOS) through the bio-e...

The objective of this study was to elucidate the role of microbial cell-to-cell communication through quorum sensing (QS) mechanisms on bioflocs characteristics and functionality in aquaculture systems. This study comprised of four experimental stages, i.e. (1) inhibition of microbial aggregation and its effect on biofloc characteristics, (2) the e...

Objective: To analyze compounds in Eleutherine bulbosa (E. bulbosa) (Mill.) Urb. extract and to determine its antibacterial capability against Vibrio parahaemolyticus (V. parahaemolyticus). Methods: E. bulbosa bulb extract was preprared using 96% ethanol by the maceration method. Phytochemical investigation of E. bulbosa extract was analyzed using...

Information on steroid hormones and fatty acids that play roles in lobster reproduction is still very limited although the data are indispensable to seed production in hatchery. The study was designed to identify steroid hormones and fatty acids during gonadal maturation of spiny lobster (Panulirus homarus). Male spiny lobsters were treated with an...