Whitney Ng

Salmonella is a common food-associated bacterium that has substantial impact on worldwide human health and the global economy. This is the public release of 1,183 Salmonella draft genome sequences as part of the 100K Pathogen Genome Project. These isolates represent global genomic diversity in the Salmonella genus.

Next Generation Sequencing (NGS) is a process that can be used to construct DNAlibraries for large-scale sequencing projects. NGS utilizes the input of high molecular weight and intact genomicDNA(gDNA) to construct high-quality libraries. The assessment ofDNAintegrity is a key step in library construction. The Agilent 2200 TapeStation System with t...

The PacBio RS II provides for single molecular, real-time (SMRT) DNA technology to sequence genomes and detect DNA modifications. The quality control methods from gDNA input to the final library using the Agilent BioanalyzerSytem and Agilent TapeStation System were evaluated. Automated protocols of PacBio 10 kb library preparation produced librarie...

Listeria monocytogenes is a food-associated bacterium that is responsible for food-related illnesses worldwide. This is the initial public release of 306 L. monocytogenes genome sequences as part of the 100K Pathogen Genome Project. These isolates represent global genomic diversity in L. monocytogenes .

Background The PacBio RS II provides for single molecule, real-time DNA technology to sequence genomes and detect DNA modifications. The starting point for high-quality sequence production is high molecular weight genomic DNA. To automate the library preparation process, there must be high-throughput methods in place to assess the genomic DNA, to e...

Automation of the PacBio 10 kb Template Preparation using the SMRTbell Template Prep kit for the sequencing of bacterial genomes on the Agilent Automated Liquid Handling Platform is described. Four bacterial genomes of differing GC content are used to prepare libraries without bias reading for next generation sequencing. The gDNA quality assessment...

Next Generation Sequencing (NGS) requires the input of high molecular weight genomic DNA (gDNA) to construct quality libraries for large scale sequencing projects , such as the 100K Pathogen Genome Project. The assessment of DNA integrity is a critical first step in obtaining meaningful data, and intact DNA is a key element for successful library c...

Next Generation Sequencing requires the input of high molecular weight genomic DNA to construct quality libraries for whole genome bacterial sequencing. Large scale sequencing projects, such as the 100K Pathogen Genome Project, require methods to rapidly assess the quantity and quality of the input DNA using high-throughput methods that are fast an...

The initial step in Next Generation Sequencing is to construct a library from genomic DNA. To gain the optimum result, extracted DNA must be of high molecular weight with limited degradation. High-throughput sequencing projects, such as the 100K Pathogen Genome Project, require methods to rapidly assess the quantity and quality of genomic DNA extra...