Wenyi Kang

Institute of Natural Products，Henan University · Pharmaceutical

In this study, ZnO nanotubes (ZNTs) were prepared onto fluorine-doped tin oxide (FTO) glass and used as supports for MIPs arrays fabrication. Due to the imprinted cavities are always located at both inner and outer surface of ZNTs, these ZNTs supported MIPs arrays have good accessibility towards template and can be used as sensing materials for che...

To establish methods for simultaneous determination of isoquercitrin, astragalin in leaves, quercetin, and kaempferol in flowers of Lysimachia clethroides Duby, respectively, the methods were ultrasound-assisted extraction combined with RP-HPLC, and ionic liquid was used as the extraction solvent. Meanwhile, the antioxidant activity of the differen...

In this work, a selective sample cleanup procedure that combined molecular imprinting technique with solid phase extraction was developed for the simultaneous extraction of the seven nitroimidazoles (NMZs) from honey samples. The molecular imprinting polymers for NMZs were prepared through bulk polymerization method using 2-methyl-5-nitroimidazole...

Incubation systems were established to investigate the effects of quercetin, kaempferol, isoquercitrin and astragalin in Lysimachia clethroides Duby on the activities of CYP2E1 and CYP3A4 in rat liver microsomes in vitro. Probe substrates of 4-nitrophenol and testosterone as well as flavonoids at different concentrations were added to the incubatio...

To study the heating treatment of Angelica dahurica under different temperature and time conditions on the stability of coumarins and tyrosinase activity. HPLC method was used to determine the contents of imperatorin and isoimperatorin, and tyrosinase activity was assayed by measuring the oxidation rate of L-DOPA in vitro. After heated, the content...

This article summarized the chemical constituents and pharmacological activities of Lysimachia L., thus providing scientific and theoretical basis for development of this genus. Literatures of experimental studies on Lysimachia genus at home and abroad were consulted and summarized. The chemical research showed that the main compounds in Lysimachia...

Plants in Ainsliaea genus, belongs to Compositae family, are traditional Chinese medicine and widely used in folk. These plants contain various types of chemical components, and main components are sesquiterpene lactone and its glycosides. In addition, there are triterpenoids, flavonoids, steroids, phenolic acid, long chain fatty acid and volatile...

In order to established a method for simultaneous determination of isoquercitrin, astragaline, quercetin and kaempferol in Lysimachia clethroides, the ionic liquid 1-hexyl-3-methylimidazolium hexafluorophosphate ([HMIM]PF6) methanol was used as the ultrasound-assisted extraction solvent combing with RP-HPLC. A Purospher star RP-C1 column was used w...

Four kinds of ionic liquids [BMIM] Br, [BMIM] BF4, [BMIM] PF6, [HMIM] PF6 were used to analyze the content of oleanic acid and paeoniflorin in Paeonia lactiflora with ultrasonic-assisted extraction coupled with HPLC. The chromatographic column, Purospher star RP-C18 (4.6 mm x 250 mm, 5 μm), was used. Acetonitrile and water (90:10) as mobile phase w...

α-Glucosidase inhibitors can be used as a new class of antidiabetic drug. By competitively inhibiting glycosidase activity, these inhibitors help to prevent the fast breakdown of sugars and thereby control the blood sugar level. This study provides a wealth of information about α-glucosidase inhibitors isolated from medicinal plants; this knowledge...

Background: Lysimachia paridiformis Var. Stenophylla mainly contain flavonoid constituents. Flavonoids and benzoquinones are the main compounds in L. fortumei Maxim. The objective of this paper was to study the volatile compounds of leaves in L. paridiformis Var. Stenophylla, L. fortumei and L. chikungensis for the first time. Materials and metho...

Background: A number of medicinal plants and there compounds played a major role in the treatment of hepatic disorders. They were widely used for the treatment of these disorders, and oxidant stress injury was one of the liver injury mechanisms. The present study evaluated the antioxidant activity and the hepatoprotective effect of each extracts o...

Background: Herbal medicines played a major role in the treatment of hepatic disorders, and a number of medicinal plants and their compounds were widely used for the treatment of these disorders, and oxidant stress injury was one of the mechanism of liver injury. Materials and methods: Antioxidant activity of Nelumbo nucifera leaves (NU) extract...

Lycopus lucidus Turcz. var. hirtus Regel belongs to the Lamiaceae family and is one of the eight species; it is widely distributed in China [1]. Plants of the genus Lycopus are rich sources of flavonoids, coumarins, terpenoids, and tannins [2, 3]. Some of them have been successfully used in folk and official medicine. The aerial parts of L. lucidus...

The chemical constituents isolated from Desmodium species (Leguminosae) included terpenoids, flavonoids, steroids, alkaloids compounds. Modem pharmacological studies have showed that the Desmodium species have antioxidant, antibacterial, anti-inflammatory, hepatoprotective, diuretic, antipyretic, analgesic and choleretic activity. This article main...

Chelerythrine is a kind of benzo[c] phenanthridine alkaloids, with such pharmacological activities as antitumor, antibiosis and anti-inflammation, which is widely found in plant of Fumariaceae, Papaveraceae, Ranunculaceae and Rutaceae families. This article summarizes the advances in domestic and foreign studies on pharmacological effect of chelery...

To study the chemical constituents from Lysimachia circaeoides. The column chromatographic techniques were applied to isolate the constituents. EI-MS and NMR were used to identify the structures. Eight compounds were isolated and identified from the extract of L. circaeoides and their structures were elucidated as kaempferol (1), beta-daucosterol (...

Twelve compounds were isolated from Psoralea corylifolia and their structures were identified as isopsoralen (1), psoralen (2), 8-methoxypsoralen (3), psoralidin (4), corylin (5), bavachin (6), daidzein (7), corylifolinin (8), bavachinin (9), neobavaisoflavone (10), daidzin (11) and astragalin (12). The results showed that psoralidin had the activi...

To study the chemical constituents from Lysimachia paridiformis. The column chromatographic techniques were applied to isolate the chemical constituents of Lysimachia paridiformis. EI-MS and NMR were used to identify the structures. Seven compounds were isolated and identified from the extract of L. paridiformis, and their structures were elucidate...

OBJECTIVE: To study the changes in physiological indices reflecting resistance to environmental stress and volatile constituents of Lysimachia nummularia Aurea. METHODS: Different concentrations (150, 200, 250 and 300 mmol · L-1) of NaCl solution was used to treat young Lysimachia nummularia Aurea to investigate the effect of saline stress on the s...

The major volatile constituents of B. chinensis flowers were aldehydes (20.4%), ketones (27.3%), esters (15.5%), and fatty acids (15.1%). The main compounds were 6-methyl-5-hepten-2-one (14.6%), isovaleraldehyde (7.9%), methyl hexadecanoate (6.7%), and n-hexadecanoic acid (6.4%). Jian et al. have separated 12 components, and 6 compounds were identi...

EtOAc and MeOH different extracts of Pimpinella candolleana Wight et Arn. have shown the α-glucosidase inhibitory and antioxidant activities when they were assayed in vitro. Chemical constituents of both extracts were isolated by column chromatography, and identified by MS and NMR spectroscopic data. Nine compounds were isolated, including 3 sterol...

To study the validity of Schizonepeta tenuifalia pieces. The change principle for the contents of essential oil and pulegone was determined by the way of classical constant temperature acceleration experiment, and the reacting speed at 20 degrees C was calculated according to Arrhenius index law and effective time was calculated. In classical const...

To study the total content change of psoralea and isopsoralea in Psoralea corylifolia under different temperature, and predict its term of validity by initial average rate stability test in order to provide the basis for assessing its quality. The reaction rate constant K was replaced by initial velocity V. According to Arrhenius rule, storage peri...

To study the content change of chlorogenic acid in honeysuckle under different temperature, and predict its term of validity at room temperature. The content of chlorogenic acid was assayed by HPLC. The activation energy E(a) and the decomposition rate constant K were calculated by initial average rate stability tests and the prediction was carried...

To study the stability of chlorogenic acid, cynaroside and 3,5-O-discaffeoylquinc acid in Hangbaiju and Gongju and to predict their term of validity. Hangbaiju and Gongju were incubated in an environmental chamber at different temperatures and relative humidities. After the incubation, quantitative determination of chlorogenic acid, cynaroside, 3,5...

To predict the stable life for Mentha haplocalyx. The volatiles in M. haplocalyx were analyzed by head-space solid micro-extraction, coupled with GC-MS and a comprehensive evaluation of essential oil in M. haplocalyx was analyzed using the factor analysis. The prediction was carried out by initial average rate stability tests using the content of e...

To study the inhibitory activity of Aeschynanthus maculatus on alpha-glucosidase. The inhibilitory model of in vitro alpha-glucosidase was established. Active extracts of A. maculatus were isolated and identified bymultiple chromatographic methods, and their molecular structures were identifiied by spectral techniques. Seven coumpounts were isolate...

To study chemical constituents in nutshell of Trapa acornis and in vitro inhibitory activity against alpha-glucosidase. EtOAC and n-butanol extractive fractions were separated by chromatography and their structures were identified by multiple spectroscopic techniques. Nine compounds were separated, they were 4,23,24-trimethylcholest-22-en-3-ol (1),...

Different solvent extracts of Hypericum ascyron L. were evaluated by α-glucosidase inhibitory activity and DPPH, ABTS and FRAP assays, respectively, for antioxidant properties in vitro. EtOAC and MeOH extracts showed stronger inhibiting activity against α-glucosidase and rat intestinal α-glucosidase compared with acarbose as positive control. Eight...

The antioxidant capacity of Indigofera stachyodes Lindl. root (ISR) extract had been determined by using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS and ferric reducing antioxidant power (FRAP) assay, and α-glucosidase inhibitory activity using 96-microplate-based method test in vitro. Extracts...

The effect of waste batteries leaching on the seedling growth and volatile constituents in leaves of Schizonepeta tenuifolia was assayed. The different concentrations of waste batteries leaching on the seedling growth were discussed. Volatile compounds were analyzed by solid-phase micro-extraction (SPME) coupled with gas chromatography-mass spectro...

Amentoflavone (AF) isolated from Selaginella tamariscina was screened for antioxidant activities in vitro, and lowering blood lipid and hepatoprotective activity in vivo. AF had no antioxidant activity in DPPH and ABTS assay and poor reducing power in FRAP assay. Intragastric administration of AF (75 mg/kg body weight per day), ethyl acetate fracti...

was 50C, held for 1 min, then programmed to 120C at a rate of 3C min –1 and held for 2 min; it was finally programmed to 210C at a rate of 4C min –1 , and held at 210C for 10 min. As a carrier gas, helium at 1.0 mL min –1 was used. The MS detector was used in the EI mode with an ionization voltage of 70 eV. The ion source temperature was 230C. The...

the flowers of L. pentapetala and account for 80.95% of the total oils. The main compounds were 3,7-dimethyl-1,6-octadien3-ol (19.51%), followed by phenylethyl alcohol (19.08%) and 1-octen-3-ol (12.60%). Among these compounds, 3,7-dimethyl-1,6-octadien-3-ol has a strong aroma of fresh grass leaves and its fragrance is widely used in perfumes, soap,...

Antibacteria activity of compounds from Puraboeo ruescens and Lysionotus pauciflorus was assayed. Disc diffusion was used to isolate compounds in vitro and berberine was positive control. The value of IC50 was assayed by the method of liquid culture. All kinds of chromatography were used to isolate the chemical constituent and structure was identif...

OBJECTIVE: To study the chemical constituents of Lysimachia clethroides Duby. METHODS: Compounds were isolated by column chromatographic techniques and their structures were elucidated by a combination of EI-MS and NMR spectroscopy. RESULTS: Seven compounds were isolated from the ethyl acetate and methanol extracts, and identified as α-amyrin(1), β...

in P. scabiosifolia flowers, which comprised 96.21% of the total volatiles. Esters (87.40%) were dominant in the flowers. The most abundant compound is cis-3-hexenyl isovalerate (28.91%), followed by pentanoic acid phenylmethyl ester (20.79%), hexyl n-valerate (10.44%), 3-methyl-2-phenylethyl butanoic acid ester (9.80%), 3-methylbutanoic acid 3-met...

A new carbamic acid, (1,7a-dihydro-1H-inden-2(7aH)-ylidene)methylcarbamic acid (1), along with three known ones, 12-ursen-28-oic acid-3-O-β-D-glucopyranoside (2), 12-ursen-3-O-β-D-glucopyranoside (3), and 3,7,11,15-tetramethyl-2-hexadecen-1-ol (4), was isolated and identified from Dryopteris wallichiana.

Export Date: 18 October 2014

Different solvent extracts of leaves and bark of Mitragyna rotundifolia (Roxb.) Kuntze were evaluated by DPPH, ABTS, and FRAP assays, respectively, for antioxidant properties. Total phenolic and flavonoid content was determined as pyrocatechol and rutin equivalents, respectively, and correlated with antioxidant activities. More polar solvent extrac...

Two new triterpenoid saponins, cincholic acid-3-O-β-D-xylopyranoside, 28-O-β-D-glucopyranosyl ester (1), quinovic acid-28-O-β-D-glucopyranosyl ester (4), and a new phenolic glucoside, 4-[4'-O-(2', 3', 5', 6'-tetrahydroxy phenyl)-β-D-glucoside]-1-butene (2), along with five known triterpenoid saponins and one phenolic glucoside were isolated from th...

The active compounds from Forsythia suspense were screened for antioxidant activities in vitro and lowering blood lipid and lipid peroxidation in vivo. Five compounds, ursolic acid, phillygenin, (+)-pinoresinol, and quercetin, from EtOAC extract and rutin from n-BuOH extract were isolated and identified by spectroscopic methods. Phillygenin and (+)...

To study the active compounds from the rhizomes of Musa basjoo. Antioxidant and alpha-glucosidase inhibitory activity of different extracts were tested. Using bioassay-guided fractionation, the chemical constituents in EtOAC extracts were isolated by column chromatography and identified by MS and NMR spectroscopy. Five compounds were isolated and i...

The alpha-glucosidase inhibitory compounds were searched from Forsythia suspense. The active compounds were isolated by the method of bioassay-guided in vitro and column chromatographic techniques. The structures of compounds were identified by MS and NMR spectroscopy. The inhibitory kinetics of the isolated compounds were investigated. The ethyl a...

To study the chemical constituents from Aeschynanthus longicaullis. The column chromatographic techniques were applied to isolate the constituents. The spectroscopie methods were used of EI-MS and NMR to identify the structures of the separated compounds. Seven compounds were isolated from the ethyl acetate extract of A. longicaullis, whose structu...

To study the chemical constituents from Corallodiscus kingianus. The column chromatographic techniques were applied to isolate constituents. A combination of ESI-MS and NMR spectroscopy was used to identify structures. Six compounds were isolated from the acetone extract of this plant, and the structures of them have been identified as castanopsol...

To study the chemical constituents and bioactivity of Teurium pilosum. Various column chromatographic techniques were used to isolate the constituents. A combination of EI-MS, NMR spectroscopy and X-Ray were applied to identify the structures. The anti-microorganism was accomplished by disk diffusion method, the antioxidant activity was assayed by...

The genus Adiantum, belonging to the Adiantaceae family, is found in mild tropical and subtropical regions. The genus consists of 200 species widespread throughout the world, mainly in South America and about 30 species in China. A. flabellulatum is a well-known traditional Chinese medicinal herb in the south of China [1]. Phytochemical research sh...

To search alpha-glucosidase inhibitors from Rubia cordifolia. The alpha-glucosidase inhibitors were isolated by the column chromatographic techniques and the bioassay-guided method in vitro. A combination of MS and NMR spectroscopy was used to identify the chemical structures. The inhibitory kinetics of the inhibitors were also investigated. The ch...

To search alpha-glucosidase inhibitors from Luculia pinciana. The alpha-glucosidase inhibitor was isolated by the column chromatographic techniques and the bioassay-guided method in vitro. A combination of MS and NMR spectroscopy was used to identify the chemical structures. The inhibitory kinetic of the isolations was also investigated. The ethyl...

A new xanthone, 3-hydroxy-1,2,5,8-tetramethoxyxanthone, along with three known ones, 1,3,8-trihydroxy-2-methoxyxanthone, 1,3,8-trihydroxy-4-methoxyxanthone and 1,3,6-trihydroxy-2,7-dimethoxyxanthone, was isolated and identified from the roots of Securidaca inappendiculata.

To study the chemical constituents from the ethyl acetate portion of an ethanolic extractive of the leaves of Aeschynanthus mengxinensis. The column chromatographic techniques were applied to isolate constituents. A combination of IR, ESI-MS, NMR and 2D-NMR spectroscopy was used to identify structures. Four compounds were isolated from the ethyl ac...

To study the antioxidant constituents from the roots of Securidaca inappendiculata. The bioassay-guided isolation of antioxidant constituents was carried out by the column chromatographic techniques. The combination of IR, MS, NMR and 2D-NMR spectroscopics methods was used to identify their structures. Result: Two new xanthones, 1, 2, 5-trihydroxy-...

A new xanthone (1, 1,7-dihydroxy-2-methoxyxanthone), in addition to the known metabolites 1,7-dihydroxyxanthone (2), 24(R)-stigmast-7,22 (E)-dien-3α-ol (3), and 1,7-dimethoxyxanthone (4), was isolated from the roots of Securidaca inappendiculata. Compounds 1–4 were evaluated by anti-HIV assay and 1–3 showed anti-HIV-1inhibitory activity in vitro.

Objective: To analyze and compare the volatile constituents from M. biondii and M. liliflora. Method: The volatile constituents were extracted by head-space solid-phase microextraction, and analyzed by GC-MS. Result: Seventy two constituents were identified from M. biondii and M. liliflora, the content of the 25 constituents in both samples we...

The essential oil of Pyrrosia petiolosa was obtained by head-space solid micro-extraction and analyzed by GC-MS for the first time. 41 compounds were detected from the essential oil of P. Petiolosa leaves, representing 95.6%, and were characterized with a high content of 2H-1- benzopyran-2-one (17.85%), followed by 10,14- trimethyl-2-pentadecanone...

The head-space (HS) volatile compounds of the buds and flowers of Prunus persica (L.) Batsch. f. duplex Rehd were analysed by solid-phase microextraction (SPME) coupled with capillary gas chromatography/mass spectrometry (GC-MS). Typical compounds detected in P. persica (L.) Batsch. f. duplex Rehd buds and flowers were alkanes. Tetradecane (4.07%),...

To study the chemical constituents from n-BuOH portion of ethanolic extract from the stem of Luculia pinceana. The column chromatographic techniques were applied to isolate constituents. A combination of IR, FAB-MS, NMR and 2D NMR spectroscopy was used to identify structures. Seven compounds were isolated from the n-BuOH fraction and their structur...

To study the chemical constituents from the bark of Mitragyna rotundifolia. Column chromatographic techniques were applied to isolate constituents. A combination of IR, MS and NMR spectroscopy was used to identify structures of constituents. Six compounds were isolated from the n-BuOH fraction and their structures were elucidated as quinovic acid-3...

Twelve compounds were isolated from the EtOAc-soluble and ButOH-soluble portions of the EtOH extract from the bark of Mitragyna rotundifolia. These compounds were identified by their spectral data as dauricine (1), 3,4-dihydroxybezoic acid (2), beta-sitosterol (3), scopleton (4),3,4,5-trimethyoxyphenol-1-glucopyranoside (5), taraxerol (6), 4-hydrox...

A new triterpene named luculiaoic acid A (1), showing inhibitory activity of a leukaemia cell line, along with eleven known compounds, has been isolated from the ethyl acetate extract of the stems of Luculia pinciana Hook. All the structures were elucidated on the basis of NMR, MS, and IR methods. The activity to inhibit Staphylococcus aureus and C...

Two new norsesquiterpenoid glycosides named austrosides A and B, together with four known glycosides, corchoionoside B, citroside A, icariside B1 and dihydrophaseic acid 4'- O-beta- D-glucopyranoside were isolated from Evodia austrosinensis. Their structures were elucidated by 1D and 2D NMR data. Austroside A showed antibacterial activity against t...

Five compounds were isolated from Neonauclea groffithii. They were benzaldoxime (1), cinnamamide (2), 3, 4-dihydroxybenzoic acid (3), scopoletin (4), jaspiterol (5). Compound 1, 2, 5 were isolated from this genus for the first time.

Two new triterpenoid saponins, quinovic acid 3-O-6-deoxy-beta-D-glucopyranoside, 28-beta-D-glucopyranosyl ester and quinovic acid 3-O-alpha-L-rhamnopyranosyl-(4 --> 1)-beta-D-glucopyranoside, along with three known saponins, have been isolated from the EtOAc extracts of the dried stems of N. sessilifolia Merr. Structure elucidation of 1-3 was based...

Seven compounds were isolated from Neonauclea sessilifolia. They are paeonol, 3,4-dihydroxybenzoic acid, scopoletin, anthraquinnones chrysophanol, 4H-1-benzopyran-4-one,5,7-dihydroxy-2-methyl, beta-sitosterol and stigmasterol glucoside. These compounds were isolated at first time from this genus and this plant.