Wen Jun Wang

Chinese Academy of Fishery Sciences | CAFS

Pyropia kinositae, a cold-water red algal species found in East Asian marine environments, thrives on aquaculture rafts and adapts to continuous submersion. Its robust growth and suitability for offshore and northern regions make P. kinositae ideal for evolving mariculture practices in China, aligning with the industry's shift towards these areas....

Climate change is altering geographic and phylogeographic distribution of macroalgae, laying great impacts on their conservation and sustainable utilization. The potential distribution of two dominant cultured seaweeds-Neoporphyra haitanensis and Neopyropia yezoensis was predicted under present and three representative concentration pathways (RCP 2...

Neopyropia yezoensis is a typical intertidal seaweed and an indispensable species for conservation of seaweed resources. As one of the most important marine vegetables, the cultivation area of N. yezoensis has been largely increasing in the past decade and ranked the second highest among all the farmed seaweeds in China. It remains unknown whether...

Salinity is a serious threat to most land plants. Although seaweeds adapt to salty environments, intertidal species experience wide fluctuations in external salinities, including hyper- and hypo-saline stress. Bangia fuscopurpurea is an economic intertidal seaweed with a strong tolerance to hypo-salinity. Until now, the salt stress tolerance mechan...

Bangia fuscopurpurea is a widespread intertidal seaweed that is commercially cultured in China. This seaweed is frequently exposed to hyposalinity stress, but little is known about the adaptation mechanisms. Ascorbate−glutathione (AsA−GSH) cycle plays important roles in many organisms under a variety of abiotic stress, including hyposaline stress....

The multiannual delayed gametophyte of kelp species maintains continuous vegetative growth under unsuitable conditions and can restart gametogenesis under suitable conditions. However, the processes and mechanisms of this phenomenon are still poorly understood. To gain insight into the processes and mechanisms of gametogenesis in multiannual delaye...

Saccharina japonica gametophytes can survive a long period under unfavorable environmental conditions, while they also delay in growth and/or reproduction. Although the reproduction in delayed gametophyte of S. japonica was known to be strongly influenced by light intensity, light quality, and photoperiod, no previous studies have evaluated their i...

Laver is the most widely farmed seaweed with the largest culture area in China. The spatio-temporal variations in composition, diversity, and functional properties of bacteria in seawater as well as the environmental variables of seawater in a large-scale laver farm in China were studied. Both the community richness indices and Shannon index in the...

Laver is a popular food for its high nutritional value, which can change among culture areas and along with the progression of harvest. Neopyropia yezoensis and Neoporphyra haitanensis were cultured in succession in Taoluo and Muping, north China. The chemical composition of laver samples together with some ecological factors in the farms were inve...

Neopyropia yezoensis is a typical intertidal seaweed and a major mariculture crops in China. The culture area of N. yezoensis has been largely increasing in the past decade. Whether large-scale cultivation of N. yezoensis has a genetic impact on wild populations remains unknown. Here, eleven polymorphic microsatellite markers were developed and app...

The purpose of this study was to develop stable microsatellite markers and evaluate the genetic background of cultivated Sargassum fusiforme. Based on the transcriptome data obtained by high-throughput sequencing, eleven polymorphic microsatellite markers were developed using four S. fusiforme populations from China. One cultivated population was f...

Neopyropia katadae is one of the important economic seaweeds in China. A mass of free-living conchocelis is needed in the commercial cultivation of N. katadae. This study aimed to clarify the optimum growth conditions of the free-living conchocelis of N. katadae and its photosynthetic and biochemical response to different temperatures and irradianc...

Caulerpa lentillifera is an economically important macroalgae. Many parental algae are needed in the commercial cultivation of C. lentillifera, and these algae are normally transported under dehydration. This study aimed to clarify the biochemical and photophysiological responses to dehydration stress to provide a theoretical basis for C. lentillif...

Background: Caulerpa lentillifera is one of the most important economic green macroalgae in the world. Increasing demand for consumption has led to the commercial cultivation of C. lentillifera in Japan and Vietnam in recent decades. Concomitant with the increase of C. lentillifera cultivation is a rise in disease. We hypothesise that epiphytes or...

Saccharina japonica is a typical seaweed that has been domesticated and cultivated at a large scale for a long time worldwide. Compared to the wild populations, the Saccharina cultivars have significant greater high-temperature tolerance. However, the molecular mechanisms of high temperature tolerance are still unknown. This study characterized the...

Bangia fuscopurpurea is a farmed species in the ancient family Bangiales. It inhabits upper intertidal zones and suffers periodical desiccation and osmotic stress. The transcriptomic regulation under dehydration and hyposalinity was investigated. The differentially expressed genes (DEGs) accounted for 18.7% of the unigenes obtained by de novo trans...

Continuous and intensive selective breeding reduces the genetic diversity of cultivated Pyropia stock, thereby increasing its susceptibility to diseases, and hindering the sustainable development of the Pyropia industry. To develop new Pyropia germplasms with desirable agronomic traits, a new Pyropia yezoensis strain, “Huangyou No. 1” was bred from...

Germplasm degeneration causes serious problems for the Chinese Pyropia yezoensis industry. A subtidal P. yezoensis strain was introduced into aquaculture. The desiccation tolerance of this strain is compared by using “periodically drying” and “never drying” culture protocols. The thalli with periodical drying tolerated ~70% relative water loss (RWL...

Pyropia dentata is a promising species for aquaculture in South China. To develop an efficient protocol for seedling culture, the growth, development, and physiological changes of P. dentata conchocelis were investigated under different temperatures, irradiances, photoperiods, and salinity. After 25 days, conchosporangia formation at 28 °C was 5–7-...

Using transcriptome data to mine microsatellite and develop markers has growingly become prevalent. However, characterizing the possible function of microsatellite is relatively rare. In this study, we explored microsatellites in the transcriptome of the brown alga Sargassum thunbergii and characterized the frequencies, distribution, function and e...

Abstract: For clarifying the development and ecological adaptability of juvenile sporophytes of Macrocystis pyrifera, the effects of photoperiod (10 L :14 D, 12 L:12 D, 14 L:10 D, 16 L:8 D) on gametophyte clone seed-rearing of Macrocystis pyrifera were studied. And, the impact of light intensity, temperature, nutrients concentration on the growth,...

Pyropia germplasm qualified for drift system culture is highly applicable in China. The current farmed species are tolerant to abiotic stresses, but vulnerable without air drying. Pyropia katadae var. hemiphylla inhabits mid-intertidal marshes and is a candidate for drift-system farming. Here, the performance of the gametophytes under varying envir...

As a temperate-cold species, Saccharina japonica often suffers heat stress when it is transplanted to temperate and subtropical zones. Study the heat stress response and resistance mechanism of Saccharina is of great significance for understanding the acclimation to heat stress under domestication as well as for breeding new cultivars with heat str...

Sargassum thunbergii is an economically important brown alga. It has been utilized as preferred food for sea cucumber in China. The present study compared the mature S. thunbergii across multiple habitats in the northern Yellow Sea. Various materials (bamboo curtains, palmropes, vinylon ropes with epoxy resin, bricks, cement stools, and mesh belts)...

New variety breeding is very significant for Saccharina japonica cultivation industry. In this paper, we reported the breeding process, evaluation of economic traits, and commercial cultivation of a new Saccharina variety "Huangguan No. 1" (variety approval number: GS-01-006-2011). The sporophytes with best performance were screened out from variet...

Sargassum thunbergii has been studied extensively because of its particular ecology and economic value. Unfortunately, further progress has been hampered by lack of available genomic information. In this study, we sequenced and de novo assembled the transcriptome of S. thunbergii. A total of 53,490,957 clean reads were produced and were assembled i...

Saccharina japonica is a cold-temperate species; it often suffers heat stress during cultivation in temperate and subtropical zone in China. In this study we investigated the response of this alga to heat stress on transcriptomic level. A total of 947 genes (1.32 %) were identified as different expression genes (DEGs), out of which 548 and 399 gene...

Saccharina japonica is one of the most important economic seaweeds. Several aspects such as photosynthesis in Saccharina lives are affected by blue light, the predominant light spectrum in the habitat. In this study, transcriptome profiling of S. japonica by next generation sequencing technology generated 55,102 qualified transcripts and 40.5 % tra...

In the present paper, we report the development of a female-specific Random Amplified Polymorphism DNA (RAPD) marker for Sargassum thunbergii gender identification using bulked segregant analysis. Five male and five female mature individuals were selected as the samples, from which the genomic DNA were extracted and mixed to produce the male DNA po...

For clarifying the physiological and ecological adaptability of leaves of Sargassum thunbergii,the photosynthesis and respiration were investigated by using liquid-phase oxygen measurement system.The major results included two aspects:(1) The differences of maximal net photosynthetic rate(Pnmax),saturation irradiance(Isat),light compensation point(...

In January and February 2010, heavy sea ice formed along the coast of the Bohai Sea and the northern Yellow Sea, China. Intertidal organisms were subjected to serious freezing stress. In this study, we investigated the freezing tolerance of the upper intertidal economic seaweed Porphyra yezoensis. The maximum photochemical efficiency of PS II (F v/...

Saccharina japonica is a brown alga that has been commercially cultured on a large scale in China. Integrating the light condition under seawater and the adaptation of Saccharina to this condition, it is expected that blue light would be beneficial to Saccharina culture system. Consequently, the detailed effect of blue light on the key stages durin...

Growth and photosynthesis in the blade of Saccharina sporophytes are strongly stimulated by blue light. However, little is known about the effect of blue light on the early development and longitudinal photosynthesis profile of Saccharina sporophytes. In this study, S. japonica sporelings were cultured under blue or red light for 8weeks. Blue light...

Bangia fuscopurpurea (Rhodophyta) was cultivated in Putian (Fujian province, China). The characteristics of the life history concerned with cultivation were investigated and the cultivation procedure was presented. The gametophytic phase (thallus) and the sporophytic phase (conchocelis) occurred alternately in the life history of B. fuscopurpurea....

The cultivars of Gracilaria lemaneiformis in Nanao (Guangdong province), Putian (Fujian) and Lianyungang (Jiangsu) all originate from the Qingdao (Shandong) wild population. After years of cultivation via vegetative propagation in marine farms in their new habitats, these cultivars vary in economic properties and morphological characters. In order...

Fucoxanthin was extracted from the intact rhizoid of Laminaria japonica Aresch with dimethyl sulfoxide (DMSO), and then recovered from the DMSO extract by partitioning into ethyl acetate and subsequent evaporation. Some isolation conditions such as solvent volume and extraction time were screened. The quantity and quality of the extracted fucoxanth...