Weiji Wang

• PhD
• Chinese Academy of Fishery Sciences

The resource of Fenneropenaeus chinensis has declined sharply due to excessive fishing intensity, ecological changes and diseases. In order to supplement the fishing yield and restore resources of F. chinensis, the relevant authorities have carried out the activities of stock enhancement and releasing. It can increase biomass and recover resources....

Analysis of environmental DNA (eDNA) has been successfully used to detect organisms in various aquatic environments. Although eDNA has been shown to persist for long periods of time in environmental samples, the efficacy of eDNA/eRNA based tools is highly dependent on degradation of the molecule, which subsequently has a great impact on the timelin...

The Yellow River estuary is an important feeding, reproduction, and development ground for a variety of marine economic species, and understanding the changes in species composition and abundance around the Yellow River estuary is of great significance. In this study, a trawl survey and seawater eDNA survey were carried out simultaneously around th...

Understanding inbreeding depressions (IBDs), the effect on the phenotypic performance of inbreeding, is of major importance for evolution and conservation genetics. Inbreeding depressions in aquatic animals were well documented in a domestic or captive population, while there is less evidence of inbreeding depression in natural populations. Chinese...

Fenneropenaeus chinensis is an important economic species in the north of China, and plays an important role in both marine fishing and aquaculture. Long-term overfishing has led to the rapid decline of wild F. chinensis resources. The traditional trawl survey could not meet the demands of the F. chinensis resource survey. In this study, environmen...

Ascites disease, caused by Edwardsiella tarda, is one of the most destructive diseases in turbot (Scophthalmus maximus). Genetic improvement of host germplasm aiming at resistance to E. tarda is the fundamental way to control this disease. However, genetic parameters for this trait in turbot are so far completely unknown, which are necessary for de...

Environmental DNA (eDNA) technology has been successfully applied to detect organisms in various aquatic ecosystems. However, eDNA has been proven to exist for a long time in environmental samples. The timeliness of eDNA detection results largely depends on the rate of molecular degradation. Environmental RNA (eRNA) is considered an excellent compl...

An introduced turbot population was used to establish families and to estimate genetic parameters of the offspring. However, there is a lack of pedigree information, and common environmental effects can be introduced when each full-sib family is raised in a single tank. Therefore, in the genetic evaluation, SSRs (simple sequence repeats) were used...

Cryptocaryonosis, caused by ciliate parasite Cryptocaryon irritans, is one of the diseases that cause large economic losses in turbot (Scophthalmus maximus). Genetic improvement of disease resistance represents one strategy for controlling infectious diseases in farmed fish. However, knowledge of whether genetic variation exists for C. irritans res...

This study provides new insights for the hatchery released Chinese shrimp (Fenneropenaeus chinensis), including proportion, dynamic migration route, after they were released into nature for stock enhancement using a new strategy quite different than ever. Chinese shrimp were sampled at 22 survey stations during two investigation voyages acrossing 7...

Turbot is an important marine aquaculture species with great economic value. Marker-assisted selection is an attractive breeding technique to improve economic traits in turbot. The objective of this study was 1)to validate SNPs associated with juvenile turbot body weight (BW)on a population scale and 2)to evaluate the performance of a marker-assist...

Genetic parameters and response to selection were estimated for harvest body weight in turbot. The data consisted of 10 952 individuals of 508 full-sib families from three generations (G0, G1, and G2). The heritability estimates for G0, G1, and G2 were 0.11±0.08, 0.18±0.09, and 0.17±0.07, respectively. Over three generations, the heritability estim...

Turbot is an economically relevant fish, and simultaneously at the risk of inbreeding depression in China. Microsatellite DNA markers were used to evaluate inbreeding level of a random turbot breeding population and further investigate its effects on growth traits. DNA based inbreeding coefficients (FDNA) were ranged from 0 to 0.4124. Average inbre...

Eight microsatellite markers were used to analyze genetic diversity, level of inbreeding, and effective population size of spawner and recaptured populations of Chinese shrimp (Fenneropenaeus chinensis) during stock enhancement in the Bohai Bay in 2013. A total of 254 and 238 alleles were identified in the spawner and recaptured populations, respec...

Ten microsatellite markers were used to analyze the levels of genetic diversity and inbreeding in a hatchery release population of Rhopilema esculentum Kishinouye (Scyphozoa: Rhizostomatidae). A total of 85 alleles were detected in 600 individuals. Within-population levels of observed (H o) and expected (H e) heterozygosity ranged from 0.152 to 0.8...

Estimates of heritability and genotype-by-environment (G × E) interactions for three common traits, such as harvest weight (HW), body length (BL), and condition factor (K) were estimated for harvest-size turbot in an industrial farming system (IFS) and a similar system maintained at a lower temperature (IFSLT). The only difference between the two e...

The mechanisms underlying sexual reproduction and sex ratio determination remains unclear in turbot, a flatfish of great commercial value. And there is limited information in the turbot database regarding genes related to the reproductive system. Here, we conducted high-throughput transcriptome profiling of turbot gonad tissues to better understand...

Bayesian and restricted maximum likelihood (REML) approaches were used to estimate the genetic parameters in a cultured turbot Scophthalmus maximus stock. The data set consisted of harvest body weight from 2 462 progenies (17 months old) from 28 families that were produced through artificial insemination using 39 parent fish. An animal model was ap...

This paper describes the development of a high density consensus genetic linkage map of a turbot (Scophthalmus maximus L.) family composed of 149 mapping individuals using Single Nucleotide Polymorphisms (SNP) developed using the restriction-site associated DNA (RAD) sequencing technique with the restriction enzyme, PstI. A total of 6,647 SNPs were...

The objectives of this present research were to assess the heritability of growth traits under low temperature conditions in turbot (Scophthalmus maximus L.), and to analyze the correlation between body weight (BW) and body length (BL). There were 536 individuals from 25 full- and half-sib families involved in this study. During the entire 90-day p...

In this study, a data set of 1140 progenies from 19 families that were composed of 13 half-sib and six full-sib families was used in the estimation of the genetic parameters of weight for juvenile turbot at 100 days post hatch. Sixty progenies were randomly selected from each family, and 20 of these individuals were genotyped at 12 microsatellite l...

We analyzed the genetic variation across six life periods that composed 3232 samples of a natural population of the Chinese shrimp Fenneropenaeus chinensis in Bohai Bay using 4 SSR (Simple Sequence Repeats) markers. Including the observed number of alleles (N a ), eight genetic variation parameters were introduced to assess the genetic diversity an...

Twenty-six half-sib groups (53 full-sib families) of turbot, Scophthalmus maximus Linnaeus, were obtained by artificial insemination. We measured growth in the offspring (40–50 individuals/family) and subjected them to a thermal tolerance challenge over a period of 34 d. There was no significant difference in daily mortality (range: 0.580%–1.391%)...

Seven microsatellite markers were used to evaluate the genetic diversity and differentiation of seven stocks of Litopenaeus vannamei, which were introduced from Central and South America to China. All seven microsatellite loci were polymorphic, with polymorphism information content (PIC) values ranging from 0.593 to 0.952. Totally 92 alleles were i...

We calculated the recapture rates for Chinese shrimp Fenneropenaeus chinensis following hatchery release in Jiaozhou Bay and Bohai Bay in 2012 based on parentage identification using simple sequence repeat (SSR) markers. The results and process were as follows: In total, 3 × 105 postlarval Chinese shrimp (inner marker), comprising six full-sib fami...

In the present study, a DNA sequence encoding a small heat shock protein gene (FcHsp21) in the Chinese shrimp, Fenneropenaeus chinensis, was cloned, and its expression was analyzed after white spot syndrome virus (WSSV) infection. The FcHsp21 gene contained an open reading frame (ORF) of 555 bp in length, encoding a 184 amino acid protein with a th...

Based on the parentage identification results using 4 polymorphic short sequence repeats (SSRs) with both parents known, we assessed the accuracy of parentage identification for a single known parent using the same 4 SSRs and 4 other additional SSRs for 2507 Chinese shrimp Fenneropenaeus chinensis recaptured from Jiaozhou Bay in a 'molecular marker...

A C-type lectin-like protein was cloned and characterized from the Chinese shrimp Fenneropenaeus chinensis, and named as FcCTL. The results indicated that the full length cDNA of 859 bp had an open reading frame encoded a polypeptide of 220 amino acids with one carbohydrate-recognition domain, six conserved Cys and one key motif EPGD. The theoretic...

In this study, totally 54 selected polymorphic SSR loci of Chinese shrimp (Fenneropenaeus chinensis), in addition with the previous linkage map of AFLP and RAPD markers, were used in consolidated linkage maps that composed of SSR, AFLP and RAPD markers of female and male construction, respectively. The female linkage map contained 236 segregating m...

Two cultured populations of "Huanghai No.1" variety and "Jikang 98" strain and four wild populations(the south coast of the Korean Peninsula population,Rushan Bay population,Qingdao coast population and Haizhou Bay population) of F.chinensis were selected as basic population via unbalanced nested breeding design in 2005.The multi-traits composite s...

This study introduced a kind of new SNP genotyping strategy in genetic analysis of Chinese shrimp (Fenneropenaeus chinensis). Twenty SNP tetra-primer ARMA-PCR primer sets were validated and used to investigate the genetic structure of six selected families of marine shrimp F. chinensis. The effective number of alleles ranged from 1.127 to 1.993, wi...

A microsatellite enrichment libraries construction colony hybridization strategy was used for large-scale isolation of microsatellites from the whole genome of the turbot (Scophthalmus maximus L.). Using 14 different probes, paired-end sequencing of 952 clones resulted in 753 positive and individual clones. Using Vector NTI Suite 8.0 and tandem rep...

Four populations of the shrimp, Penaeus chinensis, selected for White Spot Syndrome Virus resistance in breeding programs were examined at eight polymorphic microsatellite loci to assess the levels of allelic diversity and heterozygosity, as well as differentiation among the populations. A total of 71 different alleles were found over all the loci....

The β-1,3-D: -glucan binding protein (βGBP), one kind of the pattern recognition proteins (PRPs), was cloned and characterized from the Chinese Shrimp Fenneropenaeus chinensis, and named as FcβGBP-HDL. The results indicated that the full length cDNA of 6713 bp had an open reading frame encoded a polypeptide of 2139 amino acids with two glucanase-li...

Genetic linkage maps of turbot, Scophthalmus maximus L., were constructed using a “double pseudo-testcross” strategy with 158 microsatellite markers. The parents and F1 progeny (100 individuals) were used as segregating populations. We identified 21 and 30 genetic linkage groups in the male and female maps, respectively. The male linkage map was co...

Fenneropaeneus chinensis is an important species in marine fishery resources and aquaculture in China. A genetic linkage map is essential for improving the efficiency of its breeding by marker-assisted selection and identifying commercially important genes. Linkage maps of F. chinensis were constructed with an F2 mapping population (110 progenies)...

An assay was developed for the detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) based on real-time quantitative polymerase chain reaction (PCR). A pair of primers and a TaqMan probe were designed that are specific for the recognition of a conservative region in the IHHNV genome. The IHHNV real-time PCR assay had a detecti...